Author: Abdelfattah DABABAT

Activity profiling of barley vacuolar processing enzymes provides new insights into the plant and cyst nematode interaction

Abdelfattah DABABAT (2020)

Vacuolar processing enzymes (VPEs) play an important role during regular growth and development and defence responses. Despite substantial attempts to understand the molecular basis of plant–cyst nematode interaction, the mechanism of VPEs functioning during this interaction remains unknown. The second‐stage Heterodera filipjevi juvenile penetrates host roots and induces the formation of a permanent feeding site called a syncytium. To investigate whether infection with H. filipjevi alters plant host VPEs, the studies were performed in Hordeum vulgare roots and leaves on the day of inoculation and at 7, 14 and 21 days post‐inoculation (dpi). Implementing molecular, biochemical and microscopic methods we identified reasons for modulation of barley VPE activity during interaction with H. filipjevi. Heterodera filipjevi parasitism caused a general decrease of VPE activity in infected roots, but live imaging of VPEs showed that their activity is up‐regulated in syncytia at 7 and 14 dpi and down‐regulated at 21 dpi. These findings were accompanied by tissue‐specific VPE gene expression patterns. Expression of the barley cystatin HvCPI‐4 gene was stimulated in leaves but diminished in roots upon infestation. External application of cyclotides that can be produced naturally by VPEs elicits in pre‐parasitic juveniles vesiculation of their body, enhanced formation of granules, induction of exploratory behaviour (stylet thrusts and head movements), production of reactive oxygen species (ROS) and final death by methuosis. Taken together, down‐regulation of VPE activity through nematode effectors promotes the nematode invasion rates and leads to avoidance of the induction of the plant proteolytic response and death of the invading juveniles.

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HORDEUM VULGARE HETERODERA PLANT NEMATODES ENZYMES

Occurrence of the root-knot nematode species in vegetable crops in Souss region of Morocco

Abdelfattah DABABAT fouad mokrini (2018)

Root-knot nematodes ‘Meloidogyne spp’ are the most destructive group of plant parasitic nematodes causeing serious losses in vegetables crops and this damages worsened when crops grown under greenhouses conditions. In this sutdy, the distribution and characterization of root-knot nematode species collected from the Souss region of Morocco where vegetables crops intensively cultivated were determined by using both morphological and molecular tools. Out of the 110 samples collected from different greenhouses 91 (81.7%) were found to be infested with root-knot nematodes. Thirty-seven populations of root-knot nematodes were morphologically identified based on perineal patterns as well as molecularlly using species-specific primers. The obtained results indicated that Meloidogyne javanica and M. incognita were identified in 86.4% and 13.5% of the total populations, respectively. The lowest incidence of root-knot nematodes (64%) was found in Toussous, whereas the highest frequencies of 100% and 90% were detected in Taddart and Biogra, respectively. As the majority of the samples have been infested with Meloidogyne species; this indicates that there is an urgent need to provide farmers with a proper control strategy.

Article

Plant nematodes Vegetable crops Meloidogyne Occurrence Perineal Patterns Root-Knot Nematode Vegetables AGRICULTURAL SCIENCES AND BIOTECHNOLOGY PUCCINIA SORGHI GENOMES MAIZE CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Effect of seed gall nematode, Anguina tritici, on grain yield of bread wheat cultivar under field conditions

Abdelfattah DABABAT ?rfan Öztürk (2019)

The effects of Anguina tritici on wheat yields and other agronomic components were investigated at Thrace Agricultural Research Institute, Turkey, during the 2015-2016 growing seasons. In the experiment, three different gall densities were used to determine the yield loss caused by the wheat gall nematodes on Selimiye bread wheat variety. The results showed that inoculation of seed gall nematodes at 10%, 20%, and 40% infected seed resulted in yield losses of 9.7, 21.5, and 27%, respectively, when compared to the non-inoculated control. The 1,000 kernel weight did not decrease by the 3 inoculum levels (P>0.05).

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ANGUINA TRITICI PEST RESISTANCE TRITICUM AESTIVUM WHEAT NEMATODA YIELD LOSSES

Identification of Heterodera latipons using PCR with sequence characterised amplified region (SCAR) primers

MUSTAFA IMREN Abdelfattah DABABAT (2019)

The Mediterranaen cereal cyst nematode, Heterodera latipons, causing serious yield losses in protected cultivation cereal cropping areas in Turkey. An accurate and reliable identification is primarily needed to establish effective, sustainable and environmentally safe control measures to any cyst nematode species. In this study, twenty-five populations of Heterodera latipons collected from cereal areas in East-Mediterranean and South-East Anatolian regions were identified using species-specific primers (SCAR). The SCAR primer pair, H-latac F and H-latact R, defined species-specific primers were used to identify Heterodera populations. In this study stated that H-latac F and H-latact R primers for identifying of H. latipons can be efficient tools to identify the Turkish Mediterranean cereal cyst nematode populations. Moreover, the results indicated that H. latipons was the prominent cereal cyst nematode species in the East Mediterranean and South East Anatolian regions of Turkey. This clarified approach may supply a quick basis for identification of H. latipons populations for their future management and facilitate checking its distribution in the agricultural areas.

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HETERODERA IDENTIFICATION

Proceedings of the Sixth International Cereal Nematodes Symposium

Abdelfattah DABABAT fouad mokrini (2017)

The Sixth International Cereal Nematodes Symposium, held in Agadir, Morocco during 11-15 September 2017, is an update to the 1st International Cereal Cyst Nematodes Initiative Workshop held in 2009 in Antalya, Turkey. The 6 th International Cereal Nematodes Symposium involved more than 80 scientists from wheat and barley producing regions in 16 countries throughout Asia, Australia, Europe, North Africa and North America. Cereal nematodes are microscopic parasites that invade roots of wheat, barley, oats and other small grain cereals. The most important of these plant-parasitic nematodes occur in the genera Heterodera (cyst nematodes) and Pratylenchus (root-lesion nematodes). Forty five abstracts in this proceeding cover: the history and status of cereal nematodes globally and regionally; research on morphological, genetic and ecological diversity; development and deployment of host resistance including development and applications of molecular technologies; and investigations into other strategies for reducing the magnitude of economic damage caused by cereal nematodes. Special emphasis is given to opportunities to develop and deploy integrations of sustainable management practices. The papers provide valuable insights into the impacts of cereal nematodes and endeavors to provide sustainable management options for farmers. The impact of cereal nematodes in reducing crop yields and the efficiency of cropping systems ranges from severe in resource-limited cropping systems to minor in cropping systems where it is possible to integrate a broader range of rotation crops and resource-intensive inputs. Unacceptable levels of economic loss continue to occur in many countries. International collaboration such as occurred in this workshop is required to ensure that appropriate genetic resources and technologies are developed, communicated and deployed.

Conference proceedings

Wheat Cereals Nematoda CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA