Author: GLORIA MARIA MOLINA SALINAS

Further mulinane and azorellane diterpenoids isolated from Mulinum crassifolium and Azorella compacta

LUIS MANUEL PEÑA RODRIGUEZ GLORIA MARIA MOLINA SALINAS (2014)

The new mulinane diterpenoids 1 and 2 were isolated from the EtOAc extract of Mulinum crassifolium, while the rearranged mulinane 5, which was isolated for the first time from a natural source, was isolated from Azorella compacta. Compounds 1-2 were prepared by semi-synthesis thorough acetylation of the diterpene 17-acetoxymulinic acid (3). A mechanism of reaction was proposed, while the structures of the new compounds were elucidated on the basis of comprehensive spectroscopic analysis and computational methods.

Article

MULINANES AZORELLANES NEW DITERPENOIDS CHILEAN NATIVE PLANTS BIOLOGÍA Y QUÍMICA

Flourensia cernua: Hexane extracts a very active mycobactericidal fraction from an inactive leaf decoction against pansensitive and panresistant Mycobacterium tuberculosis

GLORIA MARIA MOLINA SALINAS Luis Manuel Peña Rodríguez BENITO DAVID MATA CARDENAS Fabiola Escalante Erosa DIMNA SILVIA GONZALEZ HERNANDEZ VICTOR MANUEL TORRES DE LA CRUZ HERMINIA GUADALUPE MARTINEZ RODRIGUEZ Salvador Said_Fernández (2011)

The efficacy of decoction in extracting mycobactericidal compounds from Flourensia cernua (Hojasé) leaves and fractionation with solvents having ascending polarity was compared with that of (i) ethanol extraction by still maceration, extraction with a Soxhlet device, shake-assisted maceration, or ultrasound-assisted maceration, followed by fractionation with n-hexane, ethyl acetate, and n-butanol; (ii) sequential extraction with n-hexane, ethyl acetate, and n-butanol, by still maceration, using a Soxhlet device, shake-assisted maceration, or ultrasound-assisted maceration. The in vitro mycobactericidal activity of each preparation was measured against drug-sensitive (SMtb) and drug-resistant (RMtb) Mycobacterium tuberculosis strains. The results of which were expressed as absolute mycobactericidal activity (AMA). These data were normalized to the ΣAMA of the decoction fraction set. Although decoction was inactive, the anti-RMtb normalized ΣAMA (NAMA) of its fractions was comparable with the anti-RMtb NAMA of the still maceration extracts and significantly higher than the anti-SMtb and anti-RMtb NAMAs of every other ethanol extract and serial extract and fraction. Hexane extracted, from decoction, material having 55.17% and 92.62% of antituberculosis activity against SMtb and RMtb, respectively. Although the mycobactericidal activity of decoction is undetectable; its efficacy in extracting F. cernua active metabolites against M. tuberculosis is substantially greater than almost all pharmacognostic methods.

Article

ACETIC ACID ETHYL ESTER ALCOHOL BUTANOL FLOURENSIA CERNUA DECOCTION HEXANE SOLVENT UNCLASSIFIED DRUG BIOLOGÍA Y QUÍMICA QUÍMICA BIOQUÍMICA ALCALOIDES ALCALOIDES