Author: HECTOR MANUEL MORA MONTES

Análisis de la virulencia de Sporothrix schenckii y Sporothrix brasiliensis en el organismo modelo Galleria mellonella.

DIANA MARCELA CLAVIJO GIRALDO HECTOR MANUEL MORA MONTES (2016)

La esporotricosis es una micosis subcutánea causada por un grupo de hongos patógenos filogenéticamente relacionado, conocido como el complejo Sporothrix schenckii. Hasta ahora, los estudios sobre la virulencia de estos organismos se han realizado usando modelos de infección subcutánea en ratones. En este trabajo, se llevó a cabo la estandarización de un protocolo de infección usandolarvas de G. mellonellaconcélulas deS. schenckiistricto sensu y S. brasiliensis. Para este propósito, las larvas se inocularon con las tres morfologías de este hongo (conidios, micelio y levaduras) en tres concentraciones diferentes (1x105, 1x106y 1x107células/μl) y a diferentes temperaturas. La mortalidad se monitorizó durante 15 días,que es el tiempo medio para tener una tasa de mortalidad del 100% en cada condición. El análisis estadístico reveló que las células de levadura fue lamejor morfología parallevar a cabo los análisis de virulencia, en una concentración de 1x105células/μL. Para confirmar la utilidad de este protocolo, se analizó la virulencia de nueve aislados clínicos de Sporothrix, encontrando que los datos de la virulencia en los insectos tienen una correlación del 100% con los generados en el modelo de ratón. Por lo tanto, G. mellonellaes un modelo alternativo para estudiar la virulencia delos miembros del complejoS. schenckii

Sporotrichosis is a subcutaneous mycosis caused by a group of pathogenic fungi phylogenetically related, known as Sporothrix schenckii complex. Until now, studies on the virulence of these organisms have been conducted using subcutaneous infection models in mice. In this work, we carried out the standardization of a protocol of infection of G. mellonella larvae with S. schenckii sensu stricto and S. brasiliensis cells. For this purpose, larvae were inoculated with the three morphologies of this fungus (conidia, mycelium and yeasts) in three different concentrations (1x105, 1x106 and 1x107 cells/μL) and at different temperatures for insect maintenance. Mortality was monitored during 15 days, the average time havea 100% mortality rate in each condition. Statistical analysis revealed that yeast cells was the best morphology to carry out further virulence analyzes, in a concentration of 1x105 cells/μL. To confirm the usefulness of this protocol, the virulence of nine clinical isolates of Sporothrix was analyzed, finding that the data of virulence in the insects have a 100% correlation with those generated in the mouse model. Therefore, G. mellonella is an alternative model to study the virulence of S. schenckii complex members.

Article

BIOLOGÍA Y QUÍMICA Sporothrix schenckii S. brasiliensis Galleria mellonella Virulecia.

2D-immunoblotting analysis of Sporothrix schenckii cell wall.

Estela Ruiz Baca Hector Manuel Mora Montes Everardo Lopez Romero Concepcion Toriello Najera Virgilio Mojica Marin Norma Urtiz Estrada (2011)

We utilized two-dimensional gel electrophoresis and immunoblotting (2D-immunoblotting) with anti-Sporothrix schenckii antibodies to identify antigenic proteins in cell wall preparations obtained from the mycelial and yeast-like morphologies of the fungus. Results showed that a 70-kDa glycoprotein (Gp70) was the major antigen detected in the cell wall of both morphologies and that a 60-kDa glycoprotein was present only in yeast-like cells. In addition to the Gp70, the wall from filament cells showed four proteins with molecular weights of 48, 55, 66 and 67 kDa, some of which exhibited several isoforms. To our knowledge, this is the first 2D-immunoblotting analysis of the S. schenckii cell wall.

Article

Micología Sporothrix schenckii Pared celular Glicoproteínas BIOLOGÍA Y QUÍMICA

Generation of Sporothrix schenckii mutants expressing the green fluorescent protein suitable for the study of host-fungus interactions

NANCY EDITH LOZOYA PEREZ J Sergio Casas Flores JOSE ASCENCION MARTINEZ ALVAREZ Luz Adriana López Ramírez Leila Maria Lópes Bezerra Bernardo Franco HECTOR MANUEL MORA MONTES (2018)

"Sporotrichosis is an infection caused by members of the Sporothrix genus, and among them, Sporothrix schenckii is one of the etiological agents. Both, the disease and the causative agent have gained interest in the recent years, because of the report of epidemic outbreaks, and the description of the disease transmission from animals to human beings. Despite the relevance of S. schenckii in the clinical field, there are basic aspects of its biology poorly explored. So far, Agrobacterium tumefaciens-mediated transformation has been reported as an alternative for genetic manipulation of this fungal pathogen. Here, we report the optimization of the transformation method and used this to generate insertional mutants that express the green fluorescent protein in S. schenckii. We obtained five mutant strains that showed mitotic stability and expression of the reporter gene. The strains displayed normal cell wall composition, and a similar ability to interact ex vivo with human monocytes and monocyte-derived macrophages. Moreover, the virulence in larvae of Galleria mellonella was similar to that obtained with the wild-type control strains. These data indicate that these fluorescent mutants with normal ability to interact with the host could be used in bioimaging to track the host-Sporothrix interaction in vivo."

Article

Agrobacterium tumefaciens Bioimaging Cell wall Immune sensing Transformation Virulence BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) MICOLOGÍA (SETAS) MICOLOGÍA (SETAS)

Silencing of OCH1 unveils the role of Sporothrix schenckii N-linked glycans during the host-fungus interaction

NANCY EDITH LOZOYA PEREZ J Sergio Casas Flores José Roberto Fogaça de Almeida JOSE ASCENCION MARTINEZ ALVAREZ Luz Adriana López Ramírez Grasielle Jannuzzi JOSE ELIAS TRUJILLO ESQUIVEL EINE ESTRADA MATA sandro almeida Bernardo Franco Leila Maria Lópes Bezerra HECTOR MANUEL MORA MONTES (2019)

"Background: Sporothrix schenckii is a neglected fungal pathogen for the human being and other mammals. In several fungal systems, Och1 is a Golgi alpha 1,6-mannosyltransferase with a key function in the synthesis of N-linked glycans; which are important elements during the host-fungus interplay. The role of OCH1 in fungal virulence seems to be species-specific, being an essential component for Candida albicans virulence and dispensable during the interaction of Aspergillus fumigatus with the host. Methods: Here, we silenced S. schenckii OCH1 and characterized the phenotype of the mutant strains. Results: The mutant strains did not show defects in the cell or colony morphology, the growth rate or the ability to undergo dimorphism; but the cell wall changed in both composition and exposure of inner components at the surface. When interacting with human monocytes, the silenced strains had a reduced ability to stimulate TNF alpha and IL-6 but stimulated higher levels of IL-10. The interaction with hitman macrophages was also altered, with reduced numbers of silenced cells phagocytosed. These strains showed virulence attenuation in both Galleria mellonella and in the mouse model of sporotrichosis. Nonetheless, the cytokine levels in infected organs did not vary significantly when compared with the wild-type strain. Conclusion: Our data demonstrate that OCH1 silencing affects different aspects of the S. schenckii-host interaction."

Article

Cell wall Protein glycosylation Sporotrichosis Gene silencing Host-fungus interplay Mannosyltransferase MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS MEDICINA INTERNA ENFERMEDADES INFECCIOSAS ENFERMEDADES INFECCIOSAS

Patrimonio biocultural. Experiencias integradoras

JOSE ALEJANDRO VELAZQUEZ MONTES ALEJO JAVIER LUNGO RODRIGUEZ ALICIA YANETH VASQUEZ GONZALEZ ANGEL ROLANDO ENDARA AGRAMONT CLARITA RODRIGUEZ SOTO CLOTILDE LEBRETON CONSUELO MONTALVO MARQUEZ CRISTINA GONZALEZ QUINTERO DAVID IGLESIAS PIÑA ELEUTERIO ARCOS LORETO FERMIN CARREÑO MELENDEZ FRANCISCO HERRERA TAPIA GEORGINA MARIA ARREDONDO AYALA Héctor Javier Favila Cisneros J. SANTOS HERNANDEZ ZEPEDA JESUS SALES COLIN JOSE ALFREDO CASTELLANOS SUAREZ J. ISABEL JUAN PEREZ JOSE MANUEL PEREZ SANCHEZ LAURA REYES MONTES MARCO ULISES ARCOS SANCHEZ MARIA CRISTINA CHAVEZ MEJIA MIGUEL ANGEL ESQUIVEL PEREZ NOE ANTONIO AGUIRRE GONZALEZ OCTAVIO MONROY VILCHIS PABLO PEREZ AKAKI RAFAEL DE JESUS HUACUZ ELIAS RAFAEL DIAZ LOYOLA ROCIO DEL CARMEN SERRANO BARQUIN RODRIGO MARCIAL JIMENEZ SERGIO MOCTEZUMA PEREZ VICTOR MANUEL MORA TORRES (2017)

El libro engloba diferentes perspectivas en torno al patrimonio biocultural de diferentes regiones de México, desde un contexto histórico, hasta las problemáticas político-administrativas a las que se enfrentan estás regiones. Algunos capítulos reflejan diferentes estrategias que han seguido las comunidades para rescatar ese patrimonio biocultural y avanzar hacia la sustentabilidad.

El libro fue resultado del 1er Congreso Internacional Desarrollo Sustentable: Enfoques, Aplicaciones y Perspectivas. “Ambiente, Economía, Sociedad, Territorio y Educación”. Celebrado en Toluca, Estado de México.De forma particular, el cuerpo académico sobre sustentabilidad, territorio y educación, llevo a cabo una recopilación de investigaciones en diferentes líneas de trabajo entre ellas las referentes al patrimonio biocultural y la sustentabilidad. En los trabajos aceptados se tienen experiencias que integran un sin número de aspectos que relacionan al ambiente con el patrimonio.La estructura del documento se divide en ocho capítulos y en cada uno de desarrolla la experiencia integradora del investigador.

Book

sustentabilidad desarrollo comunitario agroecología diversidad cultural CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA