Author: JORGE LUIS GONZALEZ ESCOBAR

Microbiome characterization of the escamolera ants unveils microorganisms with high biotechnological potential

JORGE LUIS GONZALEZ ESCOBAR (2018)

"Insects have adapted to several ecosystems through the versatility of their feeding habits, wherein their intestinal microbiota has played a key role in the biological processes canalization. Liometopum apiculatum ant is widely distributed in arid and semi-arid ecosystems where a relative shortage of food can be appreciated, compared to tropical ecosystems. L. apiculatum has achieved an ecological equilibrium for surviving notwithstanding environmental factors, establishing diverse symbiotic interactions with other insects and plants. However, the symbiotic relationship with microorganisms is still unknown. Therefore, the aim of this research was to characterize the microbial symbionts associated with L. apiculatum larvae and the reproductive adult ants using metagenomics, culturomics, and microscopic approaches. The most abundant Phylum identified by sequencing in the larvae was Firmicutes while in adult ants was Proteobacteria. Nonetheless, in both stage a large population of nitrogen-fixing bacteria was observed. On the other hand, the culturomics approach confirmed the existence of 19 bacteria previously identified by sequencing, which accompanied by functional characterization, revealed an important role of gut bacteria in the complex polysaccharides breakdown and releasing nutrients stored in plant-derived materials. Moreover, bacteriocytes with intracellular bacteria were detected, indicating a nutritional support through this system to ant’s metabolism. This finding highlights the important contribution of the microbial symbionts in the feeding and surviving of ants. On the other hand, the metabolic versatility of microbial symbionts showed a promising source of enzymes for potential biotechnological applications."

"Los insectos se han adaptado a diversos ecosistemas a través de la versatilidad de sus hábitos de alimentación y su microbiota intestinal juega un papel clave en la canalización de los procesos biológicos contra las perturbaciones ambientales. La hormiga Liometopum apiculatum habita en ecosistemas áridos y semiáridos caracterizados por una escasez relativa de alimentos, en comparación con los ecosistemas tropicales. No obstante, esta hormiga ha logrado un equilibrio ecológico para sobrevivir, estableciendo diversas interacciones simbióticas con otros insectos y plantas. Sin embargo, la relación simbiótica con microrganismos aún se desconoce. Por lo tanto, el objetivo de esta investigación fue caracterizar los simbiontes microbianos asociados tanto a las larvas de L. apiculatum como a las hormigas reproductoras adultas usando herramientas como la metagenómica, culturómica y microscopia. El Phylum más abundante identificado por secuenciación en las larvas fue Firmicutes, mientras que en las hormigas adultas fue Proteobacteria. En ambas etapas se observó una gran población de bacterias fijadoras de nitrógeno. Por otro lado, el enfoque culturómico confirmó la existencia de 19 bacterias previamente identificadas por secuenciación, que junto con la caracterización funcional revelaron un papel importante de las bacterias intestinales en la descomposición de polisacáridos complejos y la liberación de nutrientes almacenados en materiales derivados de plantas. Además, se detectaron bacteriocitos con bacterias intracelulares, indicando un soporte nutricional a través de este sistema para el metabolismo de las hormigas. Estos resultados destacan la importante contribución de los simbiontes microbianos en la alimentación y la supervivencia de las hormigas en su ecosistema, y además, exponen una fuente prometedora de enzimas para posibles aplicaciones biotecnológicas."

Doctoral thesis

MALDI-biotyping 16S rRNA gene Bacteriocytes Lignocellulolytic enzymes BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR

Modulation of caecal microbiome in obese mice associated with administration of amaranth or soybean protein isolates

DIANA OLGUIN CALDERON JORGE LUIS GONZALEZ ESCOBAR ROSALVA RIOS VILLA ELENA DIBILDOX ALVARADO Antonio de León Rodríguez Ana Paulina Barba de la Rosa (2019)

"Obesity is defined as abnormal or excessive body fat accumulation that may have negative effects on health. Healthy diet induces a balance of gut microbiota, helping in turn to combat this metabolic disorder. Amaranth is well known because of its beneficial properties on health. but its effects on microbiota profile are still unknown. The aim of this study was to analyse the changes of gut microbiota in diet-induced obese mice due to amaranth protein consumption and to compare them with the changes due to soybean protein intake. Male C57BL/6 mice were fed for 8 weeks with regular (RD) or high-fat (HF) diet, without or with complementation with amaranth or soybean protein isolates. Morphological changes in caecum ultrathin sections were measured after hematoxylin/eosin staining. Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. Caecal Short Chain Fatty Acids (SCEs) were quantified by gas chromatography. The consumption of soybean protein induced the ectopic deposition of fat in the whole intestine while amaranth proteins increased caecal crypt depth and calceiform cells number sustaining its beneficial effect on health. The count of Ruminococcacea family bacteria was increased in mice fed with HF diet, but amaranth proteins intake reduced its abundance. In turn, Lachnospiraceae bacteria abundance decreased in mice fed the Control-HF and amaranth HF diets. but increased in mice fed the soybean diets. In mice fed the soybean diets. amaranth induced the abundance of Prevotellaceae, an acetate-producing bacteria. Study results indicate that the modulation of caecal microbiota could be one of the mechanisms by which amaranth exerts its beneficial effects on health."

Article

Amaranth Caecal microbiome Obesity Protein intake Short-chain fatty acids BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) NUTRICIÓN NUTRICIÓN

Thermoluminescent response of C-modified Al2O3 thin films deposited by parallel laser ablation plasmas

JORGE EDUARDO VALDES GARCES LUIS ESCOBAR ALARCON PEDRO RAMON GONZALEZ MARTINEZ Dora Alicia Solís Casados JAVIER SAUL ROMERO MARQUEZ FERNANDO GONZALEZ ZAVALA EMMANUEL HARO PONIATOWSKI (2017)

Aluminium oxide thin films modified with different amounts of carbon were prepared using a parallel laser ablation plasmas configuration. The effect of the amount of carbon incorporated in the films on their compositional, morphological, structural, and thermoluminescent properties was studied. The results showed that films with different C content, from 11 to 33 at. %, were obtained. The structural characterization revealed the growth of an amorphous material. Surface morphology of the obtained thin films showed smooth surfaces. The films were exposed to UV and gamma radiation (Co-60) in order to study their thermoluminescence response. The results tend to indicate that carbon incorporation into the alumina favours the increase of a high temperature TL peak.

CONACyT CB 240998

Article

thin films laser ablation thermoluminiscence BIOLOGÍA Y QUÍMICA

The proteome map of the escamolera ant (Liometopum apiculatum Mayr) larvae reveals immunogenic proteins and several hexamerin proteoforms

José Ángel Huerta Ocampo María Soledad García Muñoz AIDA JIMENA VELARDE SALCEDO Eric Edmundo Hernández Domínguez JORGE LUIS GONZALEZ ESCOBAR Alberto Barrera Pacheco ALICIA GRAJALES LAGUNES Ana Paulina Barba de la Rosa (2018)

"The larvae of escamolera ant (Liometopum apiculatum Mayr) have been considered a delicacy since Pre-Hispanic times. The increased demand for this stew has led to massive collection of ant nests. Yet biological aspects of L. apiculatum larvae remain unknown, and mapping the proteome of this species is important for understanding its biological characteristics. Two-dimensional gel electrophoresis (2-DE) followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was used to characterize the larvae proteome profile. From 380 protein spots analyzed, 174 were identified by LC-MS/MS and homology search against the Hymenoptera subset of the NCBInr protein database using the Mascot search engine. Peptide de novo sequencing and homology-based alignment allowed the identification of 36 additional protein spots. Identified proteins were classified by cellular location, molecular function, and biological process according to the Gene Ontology annotation. Immunity- and defense-related proteins were identified including PPIases, FK506, PEBP, and chitinases. Several hexamerin proteoforms were identified and the cDNA of the most abundant protein detected in the 2-DE map was isolated and characterized. L. apiculatum hexamerin (LaHEX, GeneBank accession no. MH256667) contains an open reading frame of 2199?bp encoding a polypeptide of 733 amino acid residues with a calculated molecular mass of 82.41?kDa. LaHEX protein is more similar to HEX110 than HEX70 from Apis mellifera. Down-regulation of LaHEX was observed throughout ant development. This work represents the first proteome map as well as the first hexamerin characterized from L. apiculatum larvae."

Article

Escamoles Peptide de novo sequencing Gene ontology LC-MS/MS qRT-PCR Two-dimensional gel electrophoresis BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR

Bienestar y familia, una mirada desde la psicología positiva

ADRIANA GOMEZ BERNARDINO AIDA MERCADO MAYA BEATRIZ GONZALEZ COLIN ELIAS ALFONSO GONGORA CORONADO ERIKA ROBLES ESTRADA Eugenio Saavedra Guajardo HANS OUDHOF VAN BARNEVELD IRIS IRENE VASQUEZ VELAZQUEZ JORGE ALVAREZ MIRANDA JOSE LUIS VALDEZ MEDINA KARLA EDITH HERNANDEZ VILCHIS KORINNA GONZALEZ VAZQUEZ LOURDES GABRIELA VILLAFAÑA MONTIEL MARCELA BEATRIZ GONZALEZ FUENTES María Dolores Martínez Garduño MARIA GUADALUPE MATEO CARREÑO MARIO ULISES MAYA MARTINEZ MARITZA CASILLAS ENSASTIGA Marta Gil_Lacruz MARTHA ADELINA TORRES MUÑOZ MICAELA OLIVOS RUBIO MINERVA THALIA JUNO VANEGAS FARFANO MONICA TERESA GONZALEZ RAMIREZ NORMA IVONNE GONZALEZ ARRATIA LOPEZ FUENTES Patricia Andrade Palos SERGIO GONZALEZ ESCOBAR YESSICA PAOLA AGUILAR MONTES DE OCA (2015)

Este libro está dirigido a estudiantes y profesionales en psicología y áreas afines, como enfermería, trabajo social o educación, y a los interesados en personalidad positiva. Cada capítulo se presenta de manera sencilla y con una estructura didáctica, pero sin perder rigor científico y calidad de la revisión, con el fin de facilitar el acceso a la información sobre bienestar individual, familiar y social de una forma accesible para adentrarnos al estudio de temas de psicología positiva. Dadas las temáticas, se consideró pertinente dividir este libro en dos secciones: I. Psicología positiva y bienestar. II. Bienestar individual y familiar.

Universidad Autónoma del Estado de México y Ediciones y Gráficos Eón, S.A. de C.V.

Book

bienestar familia psicología positivo sociedad fortalezas humanas HUMANIDADES Y CIENCIAS DE LA CONDUCTA