Author: Jose Francisco Robles González

Isolation and identification of NAD-independent Avibacterium paragallinarum (serovar C-1) from growing layer hens with infectious coryza

EDGARDO SORIANO VARGAS Jesús Cabriales Jiménez JOSUE SANCHEZ MORALES VLADIMIR MORALES ERASTO Jose Francisco Robles González ALEJANDRA GARCIA SANCHEZ (2013)

The isolation of V-factor (NAD)-independent, serovar C-1 of Avibacterium paragallinarum from growing layer hens with infec- tious coryza, is reported for the first time. Av. paragallinarum serovar C-1 isolates were PCR confirmed and shared a unique genetic profile. Isolation of Av. paragallinarum NAD-independent biovar, serotyping and genotyping of isolates at the avian diagnostic laboratories are discussed.

Article

veterinaria avibacterium paragallinarum serovar c-1 v-factor mexico CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells

SANDRA MARICRUZ LOPEZ HEYDECK Marcos Cajero Juárez ROGELIO ALEJANDRO ALONSO MORALES JOSE SIMON MARTINEZ CASTAÑEDA Jose Francisco Robles González ALBERTO BARBABOSA PLIEGO JUAN CARLOS VAZQUEZ CHAGOYAN (2009)

Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment. Lipofection is a transfection method that requires lower amounts of cells and DNA than electroporation, and has proven to be effi cient in a large number of cell lines. It has been shown that after lipofection, mouse embryonic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater effi ciency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported. The objective of this work was to fi nd out if lipofection can be used as effi ciently as electroporation for regular gene targeting protocols. This context compares gene targeting effi ciency between these techniques in mouse embryonic stem cells E14TG2a, using a gene replacement type vector. No differences were found in gene targeting effi ciency between groups; however, lipofection was three times more effi cient than electroporation in transfection effi ciency, which makes lipofection a less expensive alternative method to produce gene targeting in mouse embryonic stem cells.

Article

veterinaria gene targeting homologous recombination mouse embryonic stem cells transfection electroporation lipofection CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA