Author: LUIS MIGUEL VAZQUEZ GARCIA

Fibras vegetales y las artesanías en el Estado de México

LUIS MIGUEL VAZQUEZ GARCIA GUADALUPE MUNGUIA LINO (2015)

El uso de las plantas es una práctica que siempre se ha desarrollado a la par de los grupos humanos. El propósito de este libro es difundir las principales artesanías elaboradas con fibras vegetales obtenidas de los diferentes tipos de vegetación existentes en el Estado de México; da testimonio de la elaboración de morrales de izote (Yucca treculeana), cestos de carrizo (Arundo donax) y otate (Otatea acuminata), las canastas de romerillo (Dalea lutea), las artesanías de vara de perlilla (Symphoricarpos microphyllus), el manejo del tule y junco (Typha dominguensis and Juncus arcticus), al igual que la planta alpina conocida como zacatón (Muhlenbergia macroura), el ocoxal u hoja de pino (Pinus spp.) y la elaboración de diferentes artículos derivados de la palma (Brahea dulcis). Todasestas artesanías son producto del trabajo de las comunidades rurales como una actividad complementaria de la agricultura. El escrito es una reflexión sobre la elaboración de artesanías y un análisis de la integración de esta actividad dentro de la vida cultural heredada de un pasado que tiende a ser devorada por la acelerada vida actual.

Book

Etnobotánica tradición artesanal vegetación del Estado de México CIENCIAS SOCIALES

Comparative study of the discriminating capacity of dna markers and their effectiveness in establishing genetic relationships in the genus tigridia

JESUS IGNACIO REYES DIAZ AMAURY MARTIN ARZATE FERNANDEZ JOSE LUIS PIÑA ESCUTIA LUIS MIGUEL VAZQUEZ GARCIA (2015)

Tigridia Jussieu is an endemic genus to Mexico and taxonomically difficult with limited information about its genetic variability. A diversity assessment conducted using different DNA markers as an inter simple sequence repeat (ISSR) and random amplified polymorphic DNA (RAPD) markers will be helpful in the establishment of a broad- based description for improved germplasm curation and the identification of germplasm for genome mapping and breeding of these species. Thus, the objective of this study was to characterize 15 wild species of Tigridia by using RAPD and ISSR molecular markers. This study was carried out in the laboratory of Plant Molecular Biology at the Faculty of Agricultural Sciences of the Universidad Autónoma del Estado de México between August and November of 2011. In this assay, 13 RAPD primers of 10, 15 and 20 b, and five ISSR primers of the anchored type (ASSR) of 17 b were used to assess the level of genetic variation among 15 wild species of Tigridia . With both markers there were 163 amplified bands of which 150 (92.02 %) were polymorphic. The RAPD primers of 10 b generated 12 specific bands with a polymorphism of 95.12 %, for 15 b primers those values were five and 82.93 %, and for 20 b primers eight and 94.59 %, respectively. The RAPD pooled primers presented a polymorphism of 90.76 %, the genetic distance (G D ) among the species ranged from 0.16 (between T. illecebrosa and T. huajuapanensis ) to 0.57 (between T. multiflora and T. augusta ). The ISSR primers showed more polymorphism(95.45 %) than RAPD primers. With ASSR primers the highest genetic association (G D = 0.89) was observed between T. mexicana ssp. mexicana and T. durangense , whereas the least related were T. vanhouttei spp. vanhouttei and T. multiflora (G D = 0.14). This study shows that 10 base random primers and 17 base anchored primers were more efficient to detect polymorphism and genetic differentiation among Tigridia species.

Article

Agrociencias Iridaceae RAPD ASSR molecular characterization CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

JOSE LUIS PIÑA ESCUTIA LUIS MIGUEL VAZQUEZ GARCIA AMAURY MARTIN ARZATE FERNANDEZ (2010)

Tigridia pavonia (L.f.) DC. is one of the important phytogenetic resources of México. This species is used as ornamental, food and medicinal purposes. Despite its ornamental and economic potential, there is little information about the genetic variability. In this study, randomly amplified polymorphic DNA (RAPD) primers of 10, 15 and 20 bases were used to assess the level of genetic variation among nine botanical varieties of Tigridia pavoniacollected in three localities within State of México. The total number fragments, polymorphic fragments, percentage of polymorphism and resolving power were greater for 15 base (55, 52, 94.5 and 5, respectively) and 20 base (47, 45, 95.7 and 3.8, respectively), in comparison with those obtained from 10 base primers (44, 41, 93.1 and 3.6, respectively).Resultsshowed the major effectiveness of 15 and 20 bases RAPD primers in the genetic differentiation of varieties as compared to 10 bases RAPD primers. The dendrograms based on un-weighted pair group method arithmetic average (UPGMA) analysis of the 10, 15, 20 and the pooled (10, 15 and 20) bases RAPD data were consistent in the clustering varieties, grouping them in two main clusters.

Article

Biología Genetic resources RAPD Tigridia pavonia variety discrimination BIOLOGÍA Y QUÍMICA

In vitro regeneration and genetic fidelity of Tigridia pavonia (L.f.) DC.

JOSE LUIS PIÑA ESCUTIA LUIS MIGUEL VAZQUEZ GARCIA AMAURY MARTIN ARZATE FERNANDEZ (2010)

Plants of Tigridia pavonia (L.f.) DC were regenerated from twin-scaling explants cultured on Murashige and Skoog and N6 basal medium. The highest formation of shoots per responding explant was obtained on N6 medium supplemented with 4.5 µM 2,4-dichlorophenoxy acetic acid in combination with 2.2 µM benzylaminopurine. Shoots rooted readily on N6 basal medium supplemented with 1 g l-1 activated charcoal and 2.6 µM naphtalenacetic acid. The rooted shoots achieved 100% survival. Inter Simple Sequence Repeat analysis was carried out to check for possible genetic alterations in plants obtained after two consecutive subcultures. The results revealed that the recovered plants did not exhibit any type of polymorphism.

Article

Biología Genetic integrity micropropagation oceloxóchitl BIOLOGÍA Y QUÍMICA

Biocontrol in vitro e in vivo de Fusarium oxysporum Schlecht. f. sp. dianthi (Prill. y Delacr.) Snyder y Hans. Con hongos antagonistas nativos de la zona florícola de Villa Guerrero, Estado de México

Jesús Gaudencio Aquino Martínez LUIS MIGUEL VAZQUEZ GARCIA BASILIO GABRIEL REYES REYES (2008)

Se obtuvieron 45 aislamientos de Fusarium oxysporum f. sp. dianthi y 45 de hongos antagonistas (T01- T45): Trichoderma spp. (35) y Gliocladium spp. (10), de plantaciones comerciales de clavel en Villa Guerrero, México. La actividad antagónica de las cepas se evaluó in vitro, comparada con dos cepas comerciales: T. harzianum "PHC T-22" (T46), T. lignorum "Biotrol" (T47), y una cepa nativa de T. harzianum (T48). En invernadero, se valoró la actividad enzimática de los mejores aislamientos. Las cepas nativas T20, T22, T23, T24, T25, y comercial T47 de T. lignorum presentaron diferencias altamente significativas para: invasión de colonia del patógeno, crecimiento radial del antagonista y diferencia de crecimiento radial in vitro. La actividad celulolítica de las cepas T25 y T47 fue significativamente superior en suelo sin desinfección; T47 también la presentó en suelo desinfectado. Las cepas nativas son una alternativa para controlar la "dormilona" del clavel por F. oxysporum f. sp. dianthi, fomentar el biocontrol de patógenos y reducir la aplicación de plaguicidas en la zona florícola.

Article

Agrociencias Trichoderma lignorum T harzianum hiperparasitismo CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA