Author: Manuel González Ronquillo

Chemical Composition and In Vitro Gas Production from Different Varieties of Native and Hybrid Maize Silage with the Addition of Acetic Acid or Enzymes

JOSE ANTONIO RUIZ PEREZ ADRIANA MARCELA MORENO ACOSTA Abdelfattah Zeidan Mohamed Salem OCTAVIO ALONSO CASTELAN ORTEGA Manuel González Ronquillo (2013)

The aim of this study was to evaluate and compare the chemical composition and in vitro gas production of corn white local native (WLN) corn yellow local native (YLN) and the hybrids H-51EA and CL080001 as silage, preserved by three treatments, control (CTR), the addition of acetic acid (AAC) or enzymes (ENZ). Samples were prepared in microsilos and analysed in 4x3 factorial design with three replicates of each one. The dry matter content (g/kg) was higher (P<0.01) for CLO80001 and lower for YLN (222 vs 176); organic matter (OM) content was higher (P<0.01) CLO80001 compared with the natives. Regarding treatments, OM in ENZ were higher (P<0.01) with respect to AAC and CTR; crude protein (CP) content differs by variety and treatments, WLN variety was higher (P<0.01) and the lowest CP was for CLO80001. CTR and AAC were higher in CP (P<0.01) than ENZ treatment. Neutral detergent fiber and acid detergent fiber content was higher (P<0.01) for WLN than YLN and the hybridsThe highest gas production (ml gas/g DM) (P<0.01) was for hybrids compared with local corn natives. There were no differences (P>0.05) for rate c and lag time between varieties. Dry matter disappeared (DMd) was higher (P<0.01) for CLO8001 and WLN than H51EA. ME (MJ/kg DM) was higher (P<0.01) for CLO80001 < H51EA < YLN < WLN. ME were higher (P<0.01) in ENZ and AAC than CTR. The WLN variety proves to be the best option for feeding cattle, as it turned out better than the rest of the varieties tested. Addition of corn silage with acetic acid or enzymes increased NDF digestibility and ME availability.

Article

Enzymes Acetic acid Chemical composition In vitro gas production Silage CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

PRODUCCIÓN DE FORRAJE, COMPOSICIÓN QUÍMICA Y PRODUCCIÓN DE GAS IN VITRO DE MAÍCES HÍBRIDOS AMARILLOS CULTIVADOS EN MÉXICO

Producción de forraje, composición química y producción de gas in vitro de maíces híbridos amarillos cultivados en México

LIZBETH ESMERALDA ROBLES JIMENEZ JOSE ANTONIO RUIZ PEREZ ANDRES MORALES OSORIO María de Guadalupe Gutiérrez Martínez Manuel González Ronquillo (2017)

El maíz es el forraje más importante en la alimentación del ganado, debido a su mayor contenido de energía, sin embargo, se caracteriza por su amplia gama de variedades y la posibilidad de generar una gran cantidad de productos finales. El objetivo del presente estudio fue evaluar y comparar el rendimiento, composición química y producción de gas in vitro tanto en fresco como henificado de un maíz criollo local amarillo y seis variedades de maíces híbridos amarillos (HIT13, CML460, PIONER, COBRE, CD MO80001 y CLO80902). El rendimiento en fresco y en seco no mostró diferencias entre tratamientos (P 0.05), para la composición química (g kg - 1 MS) se tuvieron diferencias (P 0.05) para el contenido de proteína por m étodo de conservación vario de 59.87 a 59.61 g kg - 1 MS , por metodo, FND de 591 a 686 g kg - 1 MS por variedad y por método de 619 a 639 g kg - 1 MS , FAD de 298 a 345 g kg - 1 MS por variedad y por método de 317 a 340 g kg - 1 MS ; LAD de 58 a 41 g kg - 1 MS por variedad y por método de 41 a 57 g kg - 1 MS respectivamente , en la producción de gas in vitro acumulada (ml gas /g MS) no se observaron diferencias (P>0.05) para la producció n total de gas entre variedades y método de conservación . Se concluye que de acuerdo con los resultados obtenidos, las varie dades estudiadas presentan los mismos rendimientos tanto en heno como en fresco. El rendimiento, la producción de gas in vitro y la producción de gas relativa no se vieron afectados por el método de conservación, ni por las variedades empleadas.

Article

Agrociencias Maíz hibrido rendimiento composición química producción de gas in vitro Agrociencias Maíz hibrido rendimiento composición química producción de gas in vitro CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Influence of Salix babylonica and Leucaena leucocephala leaf extracts on ruminal fermentation characteristics, urinary purine derivative excretion and microbial protein synthesis of lambs

PABLO MEJIA HERNANDEZ Abdelfattah Zeidan Mohamed Salem SECUNDINO LOPEZ PUENTE Xuezhao Sun ROLANDO ROJO RUBIO LUIS MIGUEL CAMACHO DIAZ MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR Manuel González Ronquillo (2014)

Extract of L. leucocephala or S. babylonica leaves was prepared as described before in Salem et al. (2011a). Briefly, leaves of each tree species were collected randomly from several young and mature trees during summer. Leaves (1.25 kg) were fresh chopped into 1 to 2 cm lengths and immediately extracted in 10 l of a solvent mixture containing 1 l of methanol, 1 l of ethanol and 8 l of distilled water. Extraction was at 25–301 C for 48–72 h in 20 l closed jars, followed by incubation at 391 C for 1 h, and then filtered. Filtrates were collected and stored at 41 C for further use. Plant extracts were weekly prepared. The 1:1 mixture extract was also prepared weekly by mixing the SB and LL extracts (0.5:0.5, vol:vol).

Sixteen growing Katahdin Pelibuey lambs (2470.3 kg body weight) were used to study the effects of oral administration of extracts of Salix babylonica (SB) and Leucaena leucocephala (LL) leaves on ruminal pH, total and individual volatile fatty acids (VFA) and NH3-N concentrations, as well as ruminal protozoal counts, urinary purine derivatives (PD) excretion and estimated microbial protein synthesis. Lambs were fed a total mixed ration (TMR) containing 219 and 141 g kg 1 of CP and NDF, respectively. Four treatments (4 lambs/treatment) were Control (no extract added), SB (SB extract at 30 ml d 1), LL (LL extract at 30 ml d 1) and SBLL (mixture of SB at 15 ml d 1þLL at 15 ml d 1). Extracts were administered orally to each lamb before the morning meal daily. Measurements were on days 22 (P1), 43 (P2) and 63 (P3) of the experiment. Ruminal pH increased (P¼0.014) with SBLL extract and decreased with SB. The ratio of acetate to propionate was higher (P¼0.042) with SBLL compared to the control. Ruminal NH3-N concentration was not affected by extract administration (P¼0.309) or experimental period (P¼0.087). Protozoal counts were not affected (P¼0.489) by extract addition but decreased (Po0.001) in P3 compared with P1 and P2. Uric acid concentration increased (Po0.001) with individual extracts of SB and LL compared with the control or SBLL. Excretion of other purine derivatives (allantoin, xanthine, and hypoxanthine), creatinine and microbial protein synthesis were not changed (P40.05) by extracts administration. Overall, oral administration of S. babylonica and L. leucocephala extracts, or their 1:1 mixture, to lambs for 63 d had only minor effects on ruminal fermentation, urinary purine derivative excretion and microbial protein synthesis.

Article

Plant extracts Rumen fermentation Purine derivatives Protozoa counts Lambs CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Effect of xylanase, cellulase and natural maguey extract on the chemical composition of corn silage and in vitro rumen gas production.

ANDRES GONZALEZ HUERTA Delfina de Jesús Pérez López RODOLFO SERRATO CUEVAS Abdelfattah Zeidan Mohamed Salem LIZBETH ESMERALDA ROBLES JIMENEZ Manuel González Ronquillo JOSÉ RAMÓN PASCUAL FRANCO MARTÍNEZ (2020)

articulo

This study considered the application of two exogenous enzymes, xylanase (XYL) and cellulase (CELL), and maguey extract (ME), (applying 1 ml per kg as fresh matter) and a control (without additive) in four maize silage varieties (San Diego, Cacahuacintle, P-1832 and Victoria), to investigate their effect on the chemical composition (CC), gas production (GP) and in vitro ruminal fermentation. The GP was measured at 0, 3, 6, 9, 12, 24, 36, 48, 72 and 96 hours of incubation. Dry matter disappearance (DMD), organic matter disappearance (OMD), metabolizable energy (ME), and short chain fatty acids (SCFAs) were determined after 96 h of incubation. Data were analyzed using a completely randomized design with a 4×4 factorial arrangement with three replications. The CC showed a significant effect (P<0.05) for varieties with the exception of organic matter (OM), and the inclusion of additives increased the dry matter (DM) and crude protein (CP). A significant effect (P<0.01) was observed for the varieties in the GP parameters and ruminal fermentation. The addition of XYL, CEL and ME promoted dry matter degradation and increased energy availability, with increased in vitro gas production.

Article

maize silage BIOLOGÍA Y QUÍMICA

Effects of Exogenous Enzymes and Salix babylonica L. Extract on Cellular Immune Response and its Correlation with Average Daily Weight Gain in Growing Lambs

Miguel Ángel Rivero Navarro Abdelfattah Zeidan Mohamed Salem Manuel González Ronquillo MARIA ANDREA CERRILLO SOTO LUIS MIGUEL CAMACHO DIAZ HANY.M. GADO CLAUDIA GIOVANNA PEÑUELAS RIVAS (2013)

The aim of this study was to determine the effects of exogenous enzyme (EZ) and Salix babylonica L. (SB) extract on cellular immune response, and its correlation with average daily weight gain (ADG). Twenty Suffolk lambs, 6-8 months of age and average live weight of 24±0.3 kg, were used in a trial which lasted 60 days. The lambs were distributed into 4 groups of 5 lambs each and housed in individual 1.5x1.5 m cages in a completely randomized design. The treatments were: (i) Control; lambs consuming basal diet (BD) only; (ii) EZ; lambs consuming BD plus 10g of EZ (ZADO®); (iii) SB; lambs consuming BD plus 30 mL of SB, and (iv) EZSB; lambs consuming BD plus 10g EZ and 30 mL of SB. Blood samples were collected on days 0, 15, 30, 45 and 60 and analysed for helper T lymphocytes, cytotoxic T limphocytes, granulocytes and monocytes by flow cytometry. Treatments had no effect on parameters measured, but day of sampling had linear and cubic effects on helper T lymphocytes, granulocytes and monocytes (P<0.01) and cubic effects on cytotoxic T lymphocytes (P<0.01). The results suggest that EZ and SB have immunosuppressant effects in the first 15 days, after this effect were immunosuppressive on cytotoxic T lymphocytes and granulocytes, for monocytes the effect was immunostimulant. No there were correlation between ADG and cellular immune response in this experiment.

Article

Exogenous enzyme Salix babylonica L. extract, Immune response Performance Lambs CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Effects of exogenous enzymes on in vitro gas production kinetics and ruminal fermentation of four fibrous feeds

MONA MOHAMED MOHAMED YASSEEN ELGHANDOUR Abdelfattah Zeidan Mohamed Salem Manuel González Ronquillo JOSE LUIS BORQUEZ GASTELUM HANY.M. GADO NICHOLAS E. ODONGO CLAUDIA GIOVANNA PEÑUELAS RIVAS (2012)

This study was conducted to investigate effects of increasing doses: 0 (control), 6 (low), 12 (medium) and 24 (high) mg/g DM of ZADO® enzyme preparation mixture (ENZ) on in vitro gas production (GP) and some ruminal fermentation parameters of the fibrous feeds Saccharum officinarum (leaves), Andropogon gayanus (leaves), Pennisetum purpureum (leaves) and Sorghum vulgare (straw). Rumen liquor was obtained from two Brown Swiss cows fitted with permanent rumen cannulae fed a total mixed ration of a 500:500 commercial concentrate and alfalfa hay ad libitum. The GP was recorded at 2, 4, 6, 8, 10, 12, 24, 48, 72 and 96 h of incubation. After 96 h, the incubation was stopped and the pH of the mixture was determined and filtrate used to determine dry matter degradability (DMD), partitioning factor (PF96), gas yield (GY24), in vitro organic matter digestibility (OMD), metabolizable energy (ME), short chain fatty acids (SCFA), and microbial crude protien production (MCP). In general, the crude protein (CP) content of the fibrous feeds was low and ranged from 23 g/kg DM (S. officinarum) to 44 (A. gayanus). The fibre contents (i.e., NDFom and ADFom) were highest (P<0.05) in S. officinarum. Increasing ENZ dose linearly increased (P<0.05) GP of all fibrous feeds and had a quadratically increased (P<0.05) asymptotic gas production in P. purpureum and S. vulgare and rate of gas production in S. officinarum and S. vulgare. Addition of ENZ also quadratically increased (P<0.05) GP at all incubation times in S. officinarum and S. vulgare, and A. gayanus, but only at 72 h in A. gayanus. The parameters of ruminal fermentation of OMD, ME, GY24 and SCFA linearly increased (P<0.05) and MCP linearly decreased (P<0.05) with the ENZ addition. Addition of enzyme affected ruminal fermentation of our feeds differently, mainly dependent on their fibre content, although dosage of enzyme was also important as impacts generally increased at higher dosages of ENZ.

Article

Fibrous feeds Gas production Enzymes Ruminal fermentation CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Effects of Calcium Soaps from Palm, Canola and Safflower Oils on Dry Matter Intake, Nutrient Digestibility, Milk Production, and Milk Composition in Dairy Goats

EINAR VARGAS-BELLO-PEREZ LIZBETH ESMERALDA ROBLES JIMENEZ Rafael Ayala Hernández JOSE ROMERO BERNAL NAZARIO PESCADOR SALAS OCTAVIO ALONSO CASTELAN ORTEGA Manuel González Ronquillo (2020)

Articulo estudio en cabras suplementadas con jabones de calcio

This study determined the e ect of protected dietary oils on dry matter intake (DMI), digestibility and milk production in dairy goats. Nine Saanen goats were used in a 3 3 Latin square design with three periods of 25 days. A basal diet based on barley hay and corn silage was supplemented with 2.7% DM of calcium soaps of either palm (PO), canola (CO) or sa ower (SO) oils. Data for dry matter intake, nutrient digestibility and milk production was analyzed using the general linear model (GLM) procedure of SAS. Gas production data was analyzed using the procedure of non-linear regression analysis (PROC NLIN) from SAS. Nutrient intakes were not a ected by treatments. However, compared with CO, the digestibility of dry matter (653 vs. 552 and 588 g/kg), organic matter (663 vs. 559 and 606 g/kg) and neutral detergent fiber (616 vs. 460 and 510 g/kg) were lowered (p < 0.001) by SO and PO. Compared with CO, in vitro gas production increased (p < 0.001) in PO and SO (174 vs. 201 and 206 mL gas/g incubated DM). Compared with PO and CO, milk production increased (p < 0.001) with SO (0.88 and 0.95 vs. 1.10 kg/d, respectively). With regard to PO and SO, CO decreased fat (34 and 35 vs. 32 g/d) and protein (35 and 38 vs. 30 g/d) in milk. In conclusion, compared to the traditional use of calcium soaps manufactured from PO, protected SO resulted in increased milk yield without negative e ects on digestibility and nutrient intake.

This research was funded by Universidad Autónoma del Estado de México (Project ID UAEMex 3060-2011 and UAEMex4974/2020).

Article

palm oil Calcium soaps goats saffower oil; CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Effect of exogenous enzymes and Salix babylonica extract or their combination on haematological parameters in growing lambs

NALLELY RIVERO PEREZ Abdelfattah Zeidan Mohamed Salem HANY.M. GADO Manuel González Ronquillo ALBERTO BARBABOSA PLIEGO CLAUDIA GIOVANNA PEÑUELAS RIVAS NICHOLAS E. ODONGO (2012)

The aim of this study was to compare the use of exogenous enzyme preparations (EZ) and/or Salix babylonica extract (SB) or their combination as feed additives on some haematological parameters in growing lambs. Twenty Suffolk lambs of 6 to 8-months-old with 24±0.3 kg body weight were used in the study. Lambs were divided into 4 groups of 5 animals each in a completely randomized design and the treatments were: 1.control: fed a basal diet of concentrate (30%) and maize silage (70%); 2. EZ: fed the basal diet plus 10 g of enzyme; 3. SB: fed the basal diet plus 30 ml of S. babylonica extract, and 4. EZSB: fed the basal diet plus 10 g enzyme and 30 ml of S. babylonica extract. Lambs were housed in individual cages and the experiment was conducted for 60 days. The SB was given orally while the EZ was mixed with a small amount of the concentrate and maize silage and was offered ad libitum. Blood samples were collected from each animal on days 0, 15, 30, 45 and 60 of experiment and analysed for haematological parameters. The treatments of EZ, SB or EZSB did not affect any of the measured blood parameters. Day of sampling modified concentrations of red blood cells (P=0.001; linear effect), haematocrit (P=0.01; quadratic effect), haemoglobin (P=0.01; linear effect), mean corpuscular volume (P=0.01; linear effect), monocytes (P=0.004; quadratic effect) and plasma protein (P=0.0002; linear effect). It could be concluded that Salix babylonica extract, exogenous enzymes and their combination as feed additives had not a negative effects on the blood parameters measured and therefore on the health of the lambs.

Article

Salix babylonica extract exogenous enzymes haemotological parameters lambs CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Physico-chemical, sensory and texture properties of an Aged Mexican Manchego-style cheese produced from hair sheep milk

JESUS ALBERTO MEZO SOLIS VICTOR MANUEL MOO HUCHIN ADRIANA SANCHEZ ZARATE Manuel González Ronquillo RACIEL JAVIER ESTRADA LEON LUIS RODRIGO IBAÑEZ ALARCON Paula Toro-Mujica ALFONSO JUVENTINO CHAY CANUL EINAR VARGAS-BELLO-PEREZ (2020)

Articulo publicado

The objective of this study was to evaluate the physicochemical and texture changes of the Manchego-style cheese produced from hair sheep (Pelibuey) throughout 180 days of ripening, as well as consumer’s acceptance. Cheese pH was constant from 1 to 180 days of ripening. Moisture, water activity, fat, elasticity and hardness decreased from day 1 to day 180, while protein, trichloroacetic acid-soluble N and free amino acid increased. Cheese lightness decreased as ripening time increased, while elasticity and hardness decreased. Principal Component Analysis was useful in discriminating cheeses according to their physicochemical composition and that allowed cheeses to be classified in two groups according to their ripening time and this resulted in those with less than 60 days and those with more than 90 days of ripening. Compared with cheeses ripened at 1 and 90 days, aged cheeses at 180 days reduced scores for appearance, color, odor, taste, texture and overall acceptance. Overall, Manchego-style cheeses from hair sheep had the usual ripened-cheese physicochemical changes.

The authors are grateful for the assistance of engineer Walter Lanz Villegas, who granted access to the facilities of the Centro de Integracion Ovina del Sureste (CIOS). We also thank D. Arcos-Alvarez, E. Bautista-Diaz, R. Espinosa-Mendoza and R. I. Narváez-Ballesteros for their technical assistance. The first author is grateful for the research grant provided by the National Council of Science and Technology of Mexico (CONACYT) for his postgraduate studies at the Universidad Juárez Autónoma de Tabasco, Mexico.

Article

sheep cheese BIOLOGÍA Y QUÍMICA

Production Performance, Nutrient Digestibility, and Milk Composition of Dairy Ewes Supplemented with Crushed Sunflower Seeds and Sunflower Seed Silage in Corn Silage-Based Diets

Eduardo Cardoso Gutiérrez Alondra Cristel Narváez López LIZBETH ESMERALDA ROBLES JIMENEZ ANDRES MORALES OSORIO María de Guadalupe Gutiérrez Martínez Esko Leskinen Marcello Mele EINAR VARGAS-BELLO-PEREZ Manuel González Ronquillo (2020)

Se evaluo la adicion de semillas de girasol, ensiladas y sin ensilar, en ovejas lecheras

This study determined production performance, nutrient digestibility, and milk composition of dairy ewes supplemented with crushed sunflower seeds (Helianthus annuus) and sunflower seed silage in corn silage-based diets. Six ewes were grouped in a double 3 × 3 Latin square design with three periods of 21 days. All treatments were based on ad libitum corn silage. Control diet was based on alfalfa hay (333 g/kg DM), sorghum grain (253 g/kg DM), triticale grain (200 g/kg DM), soybean meal (167 g /kg DM), and vitamin and mineral premix (47 g/kg DM). Sunflower seeds (SF) and sunflower seed silage (SFS) treatments consisted of alfalfa hay (333 g/kg DM), sorghum grain (267 g/kg DM), triticale grain (100 g/kg DM), soybean meal (167 g /kg DM), SF or SFS (87 g/kg DM) and vitamin and mineral premix (47 g/kg DM). Compared to control, SF and SFS increased intake and digestibility of fiber components, such as neutral detergent fiber (NDF) and acid detergent fiber (ADF). Body weight, nitrogen balance, milk yield, milk fat yield, milk protein yield, lactose yield and milk urea N were similar between treatments. Overall, results demonstrated that crushed sunflower seeds and ensiled seeds do not change significantly productive parameters of dairy sheep.

UAEM 4974/2020CIB

Article

ovino sunflower dairy digestibilidad CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA