Author: RODOLFO HERNANDEZ GUTIERREZ

BIOMARCADOR Y ANTÍGENO ASOCIADO A TUMOR: ALFA 1-ANTITRIPSINA PARA EL DIAGNÓSTICO E INMUNODIAGNÓSTICO DE CÁNCER DE MAMA EN ETAPAS TEMPRANAS

RODOLFO HERNANDEZ GUTIERREZ (2014)

La presente invención se refiere a un método para el diagnóstico in vitro de cáncer de mama en etapas I y II, el cual presenta una sensibilidad de 96% y una especificidad de 90% y cuyo método se caracteriza porque se detectan, en una muestra de suero, autoanticuerpos contra un solo antígeno asociado a tumor (AAT) que es la proteína A1AT.

Patent

MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS OTRAS ESPECIALIDADES MÉDICAS

Péptido recombinante de la porina de 42-kDa, uso como antígeno vacunal y en la detección de anticuerpos contra Histophilus somni

RODOLFO HERNANDEZ GUTIERREZ Abel Gutiérrez Ortega (2020)

En la presente invención se solicita un método para producir una respuesta inmune contra Histophilus somni en un animal mediante la administración de un péptido recombinante que corresponde a una porina de 42 kDa con una secuencia de los aminoácidos 129 al 339. También se reclama una formulación de una vacuna contra Histophilus somni que comprende un vehículo farmacéuticamente aceptable y un péptido recombinante que corresponde a una porina de 42 kDa con una secuencia de aminoaácidos del 129 al 339.

Patent

BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Identification of immunodominant proteins from Mannheimia haemolytica and Histophilus somni by an immunoproteomic approach

RODOLFO HERNANDEZ GUTIERREZ ANGEL HILARIO ALVAREZ HERRERA ABEL GUTIERREZ ORTEGA (2015)

"Mannheimia haemolytica and Histophilus somni are frequently isolated from diseased cattle with bovine respiratory disease (BRD).

They compromise animal lung function and the immune responses generated are not sufficient to limit infection. Identification of specific immunogenic antigens for vaccine development represents a great challenge. Immunogenic proteins were identified by immunoproteomic approach with sera from cattle immunized with a commercial cellular vaccine of M. haemolytica and H. somni.

Proteins of M. haemolytica were identified as solute ABC transporter, iron-binding protein, and hypothetical protein of capsular

biosynthesis. Histophilus somni proteins correspond to porin, amino acid ABC transporter, hypothetical outer membrane protein,

cysteine synthase, and outer membrane protein P6. Although these antigens share strong similarities with other proteins from animal pathogens, the ABC system proteins have been associated with virulence and these proteins could be considered as potential vaccine candidates for BRD."

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Optimización de la Asignación del Personal Docente con Base en una Evaluación Integral

RODOLFO ESPINDOLA HEREDIA ENOC GUTIERREZ PALLARES JESUS EDMUNDO LOPEZ HERNANDEZ MARCO ANTONIO PEREZ MENDEZ (2016)

Resumen: El desempeño del personal docente en la UAP Izcalli, actualmente se mide en conformidad con tres criterios importantes: 1) La apreciación del profesor realizada por los estudiantes, 2) La evaluación de la clase metodológica y 3) Visita a clase por personal del área académica durante el período. Esta información aunque útil, no es utilizada para la asignación de la carga laboral del docente en el período siguiente. En el presente trabajo, presentamos un modelo de programación lineal donde proponemos integrar toda la información disponible sobre el desempeño docente, de tal forma, que la asignación de profesores considere todas los aspectos; tanto de evaluación cumplimiento, así como sus restricciones. Esta propuesta de asignación, garantizará la optimización del personal docente y al mismo tiempo se reflejará en un mayor aprovechamiento del estudiante. Abstract: The competence of teachers in the UAP Izcalli currently mesuared according to three major criteria: 1)Teacher appreciation by nstudents, 2)Evaluation of methodological class and ·3) visit to class by the academic are during the period. This information, While useful, But is not used for allocating the workload of teachers in the next period. In this paper, we present a linear programming model which we propose to integrate all available information on teacher performance, so that the allocation of teachers considers all aspects; Therefore evaluation, compliance and restrictions. This proposal will ensure the optimization allocation of teachers and at the same time will be reflected in highter student achievement.

Book part

Educación Universitaria Investigación Operacional Administración Docente CIENCIAS SOCIALES

Immune response to a potyvirus with exposed amino groups available for chemical conjugation

CARLOS ALBERTO MANUEL CABRERA ANA LAURA MARQUEZ AGUIRRE RODOLFO HERNANDEZ GUTIERREZ Pablo César Ortiz Lazareno Gabriela Chávez Calvillo MAURICIO CARRILLO TRIPP LAURA SILVA ROSALES Abel Gutiérrez Ortega (2012)

"Background:

The amino terminus of the tobacco etch virus (TEV) capsid protein is located on the external surface of infectious TEV particles, as proposed by previous studies and an in silico model. The epsilon amino groups on the exposed lysine residues are available for chemical conjugation to any given protein, and can thus act as antigen carriers. The availability of amino groups on the surfaces of TEV particles was determined and the immune response to TEV evaluated.

Results:

Using a biotin-tagged molecule that reacts specifically with amino groups, we found that the TEV capsid protein has amino groups on its surface available for coupling to other molecules via crosslinkers. Intraperitoneal TEV was administered to female BALB/c mice, and both their humoral and cellular responses measured. Different IgG isotypes, particularly IgG2a, directed against TEV were induced. In a cell proliferation assay, only spleen cells from vaccinated mice that were stimulated in vitro with TEV showed significant proliferation of CD3+/CD4+ and CD3+/CD8+ subpopulations and secreted significant amounts of interferon g.

Conclusions:

TEV has surface amino groups that are available for chemical coupling. TEV induces both humoral and cellular responses when administered alone intraperitoneally to mice. Therefore, TEV should be evaluated as a vaccine adjuvant when chemically coupled to antigens of choice.

Keywords: Tobacco etch virus, capsid protein, amino groups, chemical conjugation, immune response."

Article

BIOLOGÍA Y QUÍMICA BIOLOGÍA Y QUÍMICA

Self-assembly of hexahistidine-tagged tobacco etch virus capsid protein into microfilaments that induce IgG2-specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein

CARLOS ALBERTO MANUEL CABRERA ALBA ADRIANA VALLEJO CARDONA EDUARDO PADILLA CAMBEROS RODOLFO HERNANDEZ GUTIERREZ SARA ELISA HERRERA RODRIGUEZ ABEL GUTIERREZ ORTEGA (2016)

"Abstract

Background: Assembly of recombinant capsid proteins into virus-like particles (VLPs) still represents an interesting

challenge in virus-based nanotechnologies. The structure of VLPs has gained importance for the development and

design of new adjuvants and antigen carriers. The potential of Tobacco etch virus capsid protein (TEV CP) as adjuvant

has not been evaluated to date.

Findings: Two constructs for TEV CP expression in Escherichia coli were generated: a wild-type version (TEV-CP) and a

C-terminal hexahistidine (His)-tagged version (His-TEV-CP). Although both versions were expressed in the soluble

fraction of E. coli lysates, only His-TEV-CP self-assembled into micrometric flexuous filamentous VLPs. In addition,

the His-tag enabled high yields and facilitated purification of TEV VLPs. These TEV VLPs elicited broader IgG2-

specific antibody response against a novel porcine reproductive and respiratory syndrome virus (PRRSV) protein

when compared to the potent IgG1 response induced by the protein alone.

Conclusions: His-TEV CP was purified by immobilized metal affinity chromatography and assembled into VLPs,

some of them reaching 2-μm length. TEV VLPs administered along with PRRSV chimeric protein changed the

IgG2/IgG1 ratio against the chimeric protein, suggesting that TEV CP can modulate the immune response against

a soluble antigen".

Article

virus del grabado del tabaco, proteína de la cápside, partículas pseudovíricas, etiqueta de hexahistidinas, adyuvante, proteína quimérica BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR

Immune response to a potyvirus with exposed amino groups available for chemical conjugation

CARLOS ALBERTO MANUEL CABRERA ANA LAURA MARQUEZ AGUIRRE RODOLFO HERNANDEZ GUTIERREZ PABLO CESAR ORTIZ LAZARENO GABRIELA CHAVEZ CALVILLO MAURICIO CARRILLO TRIPP LAURA SILVA ROSALES ABEL GUTIERREZ ORTEGA (2017)

"Background: The amino terminus of the tobacco etch virus (TEV) capsid protein is located on the external surface of infectious TEV particles, as proposed by previous studies and an in silico model. The epsilon amino groups on the exposed lysine residues are available for chemical conjugation to any given protein, and can thus act as antigen carriers. The availability of amino groups on the surfaces of TEV particles was determined and the immune response to TEV evaluated.

Results: Using a biotin-tagged molecule that reacts specifically with amino groups, we found that the TEV capsid protein has amino groups on its surface available for coupling to other molecules via crosslinkers. Intraperitoneal TEV was administered to female BALB/c mice, and both their humoral and cellular responses measured. Different IgG isotypes, particularly IgG2a, directed against TEV were induced. In a cell proliferation assay, only spleen cells from vaccinated mice that were stimulated in vitro with TEV showed significant proliferation of CD3+/CD4+ and CD3+/CD8+ subpopulations and secreted significant amounts of interferon g.

Conclusions: TEV has surface amino groups that are available for chemical coupling. TEV induces both humoral and cellular responses when administered alone intraperitoneally to mice. Therefore, TEV should be evaluated as a vaccine adjuvant when chemically coupled to antigens of choice".

Article

BIOLOGÍA Y QUÍMICA

Probiotics and Prebiotics as a Therapeutic Strategy to Improve Memory in a Model of Middle-Aged Rats.

Alejandra Romo Araiza Gabriela Gutierrez Salmean Emilio Jorge Gonzalez Galvan Melissa Guadalupe Hernandez Frausto Gabriel Herrera Lopez Hector Manuel Romo Parra Rodolfo Garcia Contreras Ana Maria Fernandez Presas Ricardo Jasso Chavez Cesar Borlongan (2018)

Aging is associated with morphological, physiological and metabolic changes, leading to multiorgan degenerative pathologies, such as cognitive function decline. It has been suggested that memory loss also involves a decrease in neurotrophic factors, including brain-derived neurotrophic factor (BDNF). In recent years, microbiota has been proposed as an essential player in brain development, as it is believed to activate BDNF secretion through butyrate production. Thus, microbiota modulation by supplementation with probiotics and prebiotics may impact cognitive decline. This study aimed to evaluate the effects of probiotics and prebiotics supplementation on the memory of middle-aged rats. Sprague-Dawley male rats were randomized in four groups (n = 13 per group): control (water), probiotic (E. faecium), prebiotic (agave inulin), symbiotic (E. faecium + inulin), which were administered for 5 weeks by oral gavage. Spatial and associative memory was analyzed using the Morris Water Maze (MWM) and Pavlovian autoshaping tests, respectively. Hippocampus was obtained to analyze cytokines [interleukin (IL-1?) and tumor necrosis factor (TNF-?)], BDNF and ?-aminobutyric acid (GABA) by enzyme-linked immunosorbent assay (ELISA). Butyrate concentrations were also evaluated in feces. The symbiotic group showed a significantly better performance in MWM (p < 0.01), but not in Pavlovian autoshaping test. It also showed significantly lower concentrations of pro-inflammatory cytokines (p < 0.01) and the reduction in IL-1? correlated with a better performance of the symbiotic group in MWM (p < 0.05). Symbiotic group also showed the highest BDNF and butyrate levels (p < 0.0001). Finally, we compared the electrophysiological response of control (n = 8) and symbiotic (n = 8) groups. Passive properties of CA1 pyramidal cells (PCs) exhibited changes in response to the symbiotic treatment. Likewise, this group showed an increase in the N-methyl-D-aspartate receptor (NMDA)/AMPA ratio and exhibited robust long-term potentiation (LTP; p < 0.01). Integrated results suggest that symbiotics could improve age-related impaired memory.

Article

Memoria asociativa Butirato Memoria espacial Probióticos Prebióticos MEDICINA Y CIENCIAS DE LA SALUD

Functional properties of Ditaxis heterantha proteins

MARIA EUGENIA JARAMILLO FLORES RODOLFO HERNANDEZ GUTIERREZ JUAN CARLOS MATEOS DIAZ HUGO ESPINOSA ANDREWS Ana Paulina Barba de la Rosa JOSE OCTAVIO RODILES LOPEZ SOCORRO JOSEFINA VILLANUEVA RODRIGUEZ EUGENIA DEL CARMEN LUGO CERVANTES (2013)

"Ditaxis heteranthais a plant of the Euphorbiaceae family that grows in semiaridregions of Mexico. It produces yellow pigmented seeds that are used for color-ing of foods. The seeds contain about 20% of proteins. Proteins ofD. heteran-thawere extracted and fractionated on the basis of solubility. Three mainprotein fractions were obtained: glutelins, 488+-0.5; albumins, 229+-2; andtotal globulins, 160+-1 g/kg. The amino acid profile was evaluated for eachfraction and protein isolated, where the protein isolate contains essential aminoacids such as Val, Phe, Tyr, and Leu. A calorimetric study showed that globu-lins and glutelins have a high denaturing temperature between 100 and 106°C,while albumins showed a denaturing temperature at 76°C. The protein isolateand its fractions exhibited functional properties: the isolated protein demon-strated good oil-holding capacity of 40.7 g/kg. Foam capacity (FC) and foamstability (FS) were observed principally in glutelins and globulins where FCmaximum was 330% and the FS was 28 min. The emulsifying capacity wasobserved in the same fractions of glutelins and globulins, followed by albumins.However, the glutelin fraction in particular was the only fraction that exhibitedemulsifying stability at pH 5, 6, and 7. Gelling capacity was observed in albu-mins and globulins. This study indicated that protein isolated fromD. heteran-thacould be used in food formulations due to its essential amino acid profile.Glutelin could be used as an emulsifying additive. Additionally, glutelin andglobulin were stable at temperatures above 100°C; this is an important factor infood industry, principally in heat processes".

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Agave bagasse biorefinery: processing and perspectives

Rodolfo Palomo Briones Irma López Gutiérrez FABIOLA ISLAS LUGO KAREN LIZETH GALINDO HERNANDEZ Dendera Munguía Aguilar JACK ANDRES RINCON PEREZ MIGUEL ANGEL CORTES CARMONA Felipe Alatriste Mondragón Elías Razo Flores (2018)

"Agave bagasse (AB) is the main solid waste generated by the tequila industry in Mexico, which is an environmental concern due to its considerable volume of production (377,000 Ton in 2016). AB is a lignocellulosic biomass that has been considered as a potential feedstock for different industrial uses in the framework of lignocellulosic biorefinery concept. The lignocellulosic biomass is a complex structure constituted by cellulose, hemicellulose and lignin. Therefore, for a complete waste revalorization, different processing steps would be required. In this work, the scientific advances toward the AB biorefinery composed by three sequential stages: pretreatment, treatment and biofuels production were reviewed. Moreover, the by-products of the process could also be recovered and used for the synthesis of value-added products. This integrative approach of AB in the conceptualized biorefinery generates positive impacts on environment as well as on local and regional economies."

Article

Biorefinery Agave bagasse Lignocellulosic biomass By-products Biofuels INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS CIENCIAS TECNOLÓGICAS