Evaluación de un derivado de la Adenosina sobre la metástasis ósea inducida por el cáncer de mama

Evaluation of an Adenosine derivative on breast cancer induced bone metastasesEvaluation of an Adenosine derivative on breast cancer induced bone metastases

Citlalli Oyuki Mendoza Chacón (2024, [Tesis de maestría])

El cáncer de mama (CM) es considerado como el tipo de cáncer con mayor incidencia y la principal causa de muerte en mujeres en el mundo. Cuando el cáncer está avanzado y ha migrado a otros órganos y específicamente a los huesos, se conoce como metástasis ósea. Los tratamientos actuales ofrecen a los pacientes un panorama favorable cuando el cáncer está en estadio temprano; sin embargo, aún no existe una terapia que cure la metástasis ósea. La adenosina y sus derivados han demostrado su potencial en la regulación del crecimiento tumoral y la supervivencia de las células cancerosas. Por lo tanto, este trabajo tuvo como objetivo evaluar el potencial anticancerígeno de un derivado de la adenosina (IFC 305) sobre células de cáncer de mama 4T1 in vitro mediante ensayos de proliferación, migración, ciclo celular y apoptosis; así mismo, su efecto en un modelo in vivo de metástasis ósea. Los datos demuestran que el fármaco IFC 305 disminuye la proliferación y migración de la línea celular 4T1 dependiendo de la concentración utilizada. Por otro lado, no induce apoptosis a las 24 horas de tratamiento, pero sí un arresto del ciclo celular en la fase G0/G1. Sin embargo, el tratamiento con IFC 305 de ratones con metástasis ósea, no tuvo efecto en la disminución de las lesiones osteolíticas. Estudios futuros son necesarios para optimizar la dosis y tratamiento necesario para el modelo in vivo. En conclusión, nuestro trabajo demostró el potencial anticancerígeno del IFC 305 en ensayos in vitro, a través de la inhibición del crecimiento celular y la migración de las células cancerígenas, y sienta las bases para futuros estudios in vivo que demuestren su potencial terapéutico para el tratamiento de la metástasis ósea causada por el cáncer de mama.

Breast cancer is the type of cancer with the highest incidence and the leading cause of death in women due to this condition. When cancer cells migrate to other organs, especially bones, it is known as bone metastasis. Although the prognosis for some patients may be favorable due to early detection and treatment, there is still no therapy to cure bone metastasis by breast cancer. Adenosine and its derivatives have demonstrated their relevance in controlling proliferation, immune response, and apoptosis in different types of cancer. In this project, the anticancer potential of the drug IFC 305, a derivative of adenosine, on bone metastasis induced by breast cancer was evaluated both in vitro and in vivo. To evaluate the effect of IFC 305 on cell proliferation and migration of the 4T1 murine breast cancer cell line, the MTT assay and wound healing assay were used, respectively. The drug inhibited the proliferation and migration of 4T1 cells in a dose-dependent manner. To understand the underlying mechanism, apoptosis and the cell cycle were analyzed by flow cytometry. Results showed that after 24 hours of exposure to the drug, the effect on apoptosis is minimal. However, it was found that 4T1 cells were arrested in the G0/G1 phase of the cell cycle. However, IFC 305 treatment of mice with bone metastasis did not reduce osteolytic lesions. Future studies are necessary to optimize the dose and treatment necessary for the in vivo model. In conclusion, our work demonstrates the anticancer potential of IFC 305 on in vitro assays, through the inhibition of cell growth and migration of cancer cells. These studies seed the bases for future in vivo studies that demonstrate the IFC 305 therapeutic potential for the treatment of bone metastasis caused by breast cancer.

cáncer de mama, tratamientos, derivado de adenosina, metástasis Breast cancer, treatments, adenosine derivate, metastasis BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA CELULAR OTRAS OTRAS

Diseño de una red de dispositivos inalámbricos para monitorear la calidad del aire en interiores

FRIDA JENNY DE LA ROSA ANDRADE (2023, [Tesis de maestría])

No cabe duda de que la contaminación ambiental siempre a existido, y es una consecuencia

producida por las diferentes actividades que el ser humano ha realizado a lo largo de su

desarrollo y evolución tecnológica, por lo que conlleva a muchas repercusiones en la

integridad física del ambiente. Las actividades de desarrollo, como la construcción, el

transporte y la fabricación, no solo agotan los recursos naturales, sino que también producen

una gran cantidad de desechos que conducen a la contaminación del aire, el agua, el suelo y

los océanos, dando pie a los problemas más preocupantes del ser humano, tal es el caso del

calentamiento global, las lluvias acidas. Hoy en día la contaminación del aire se considera

un tema de gran importancia puesto que, es uno de los principales problemas en las zonas

más urbanizadas del mundo, y se encuentra presente tanto en los países desarrollados como

en los no desarrollados, por ello surge la necesidad de conocer que tan contaminado se

encuentra el aire que se respira.

El estándar de calidad del aire es una táctica para establecer las condiciones del aire desde un

grado de pureza, hasta una calidad critica perjudicial para el deterioro de la salud humana,

haciendo referencia a la cantidad de contaminación presente en el aire, definiéndola ya sea

de alta calidad con un nivel bajo de contaminación o una mala calidad con un nivel elevado

de concentración de contaminación en el aire.

En este proyecto se diseñará una Red de dispositivos inalámbricos para el análisis y

monitoreo de la calidad del aire en interiores, con la finalidad de fungir como un medidor de

contaminación que le permita a las personas darse cuenta de que tan contaminados están sus

hogares. Por tal motivo en el desarrollo de este proyecto se hará uso de un microcontrolador

Arduino IDE, un Node-red y un phpMyadmin para el análisis y monotoreo constante de

diversos gases como CO, CO2, metano, nitrógeno y O2. Cabe mencionar que este escrito se

ha dividido en 4 capítulos en los cuales se explica detalladamente todos y cada uno de los

procesos llevado a cabo para la elaboración y el desarrollo de este proyecto.

There is no doubt that environmental pollution has always existed, and is a consequence

produced by the different activities that human beings have carried out throughout their

development and technological evolution, which leads to many repercussions on the physical

integrity of the environment. Development activities such as construction, transportation and

manufacturing not only deplete natural resources but also produce a large amount of waste

leading to pollution of air, water, soil and oceans, giving rise to to the most worrying problems

of human beings, such as global warming and acid rain. Nowadays, air pollution is considered

an issue of great importance since it is one of the main problems in the most urbanized areas

of the world, and is present in both developed and undeveloped countries, which is why the

need to know how contaminated the air you breathe is.

The air quality standard is a tactic to establish air conditions from a degree of purity to a

critical quality harmful to the deterioration of human health, referring to the amount of

pollution present in the air, defining it either high quality with a low level of contamination

or poor quality with a high level of concentration of contamination in the air.

In this project, a network of wireless devices will be designed for the analysis and monitoring

of indoor air quality, with the purpose of serving as a pollution meter that allows people to

realize how polluted their homes are. For this reason, in the development of this project, an

Arduino IDE microcontroller, a Node-red and a phpMyadmin will be used for the analysis

and constant monitoring of various gases such as CO, CO2, methane, nitrogen and O2. It is

worth mentioning that this writing has been divided into 4 chapters in which each and every

one of the processes carried out for the preparation and development of this project is

explained in detail.

BIOLOGÍA Y QUÍMICA QUÍMICA Red de dispositivos inalámbricos, análisis monitoreo, calidad, riesgo, contaminación. Network of wireless devices, monitoring analysis, quality, risk, contamination.

Fitorremediación asistida a través de bacterias, para suelos contaminados con metales pesados y plaguicidas

TANIA GÓMEZ MORALES (2023, [Otro, Trabajo terminal, especialidad])

El suelo es un recurso no renovable que nos proporciona diferentes beneficios, incluidos los servicios ecosistémicos. Sin embargo, actividades antropogénicas como la minería y la agricultura lo han contaminado. Las prácticas mineras y agrícolas liberan contaminantes como plaguicidas y metales pesados al suelo como contaminantes individuales o mezclas, generando importantes efectos adversos para el ambiente y la salud humana. Debido a esta problemática, se han desarrollado diferentes estrategias de remediación para sitios con presencia de estos co-contaminantes. Una de ellas es la fitorremediación asistida por bacterias, una técnica basada en la asociación de plantas y bacterias, donde ambos organismos se benefician y mejoran la eficiencia de la remediación.

Soil is a non-renewable resource that provides us with different benefits, including ecosystem services. However, anthropogenic activities such as mining and agriculture have contaminated it. Minery and agriculture practices release contaminants such as pesticides and heavy metals into the soil as individual pollutants or mixtures, generating important adverse environmental and human health effects. Due to this problem, different remediation strategies have been developed for sites with the presence of these co-contaminants; one of them is bacterial-assisted phytoremediation, a technique based on the association of plants and bacteria, where both organisms benefit and improve remediation efficiency.

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA suelo, fitorremediación, bacterias, plaguicidas, metales pesados, co-contaminados. soil, phytoremediation, bacteria, pesticides, heavy metals, co-contaminated

PROPUESTA DE MANEJO DE LA PESQUERÍA EMERGENTE DE MERLUZA DEL PACÍFICO NORTE Merluccius productus EN EL GOLFO DE ULLOA, BAJA CALIFORNIA SUR

PABLO MORA ZAMACONA (2023, [Tesis de doctorado])

"La merluza del Pacífico Merluccius productus se distribuye desde Alaska hasta el sur de México, con una población particular descrita al sur de la península de Baja California conocida como merluza enana, de la que poco se sabe. Dicha población actualmente no es pescada comercialmente, por lo que representa un recurso con potencial de explotación. Las pesquerías potenciales y emergentes crean desafíos y oportunidades para los administradores pesqueros que necesitan tomar decisiones sobre cómo gestionar de forma sostenible una pesquería previa a su inicio. Este trabajo propone y analiza posibles medidas alternativas de gestión pesquera para esta potencial pesquería, bajo incertidumbre biológica y de mercado. Debido al desconocimiento de diversos aspectos de la biología del recurso primeramente se analizó el crecimiento individual del stock y posteriormente se realizó un análisis bioeconómico de la potencial pesquería.

Para el análisis del crecimiento individual se utilizó un total de 240 otolitos y datos de longitud patrón de 932 especímenes muestreados de mayo a diciembre de 2015. La formación anual de incrementos en los otolitos se verificó mediante la relación de incremento marginal y el análisis de borde. La edad se estimó a partir de recuentos de anillos en secciones sagitales de otolitos y el crecimiento se analizó mediante un enfoque de inferencia multimodelo. Se consideraron los modelos de crecimiento de von Bertalanffy (VBGM), VBGM generalizado, Gompertz, Logístico y Johnson, los cuales se ajustaron a conjuntos de datos de talla a la edad observados y retrocalculados agrupados por sexo. Según el criterio de información de Akaike, el VBGM generalizado fue el más apropiado para las hembras (L∞= 31.36 cm, k= 0.15 cm año-1), mientras que el VBGM proporcionó el mejor ajuste para los machos (L∞= 25.35 cm, k= 0.28 cm año-1).

El análisis bioeconómico de alternativas de manejo para una potencial pesquería de merluza enana indicó que una nueva pesquería de dicho stock podría ser biológicamente sostenible y económicamente rentable, bajo un conjunto de estrategias de gestión y reglas de control. Se recomienda un acceso limitado con bajo esfuerzo pesquero ya que resulta más redituable por barco y biológicamente precautorio, considerando la alta incertidumbre asociada con la explotación de una población virgen..."

"The Pacific hake Merluccius productus is distributed from Alaska to southern Mexico, with a particular population described south of the Baja California peninsula known as dwarf hake, about which little is known. This population is not currently commercially fished; hence it represents a resource with potential for exploitation. Potential and emerging fisheries create challenges and opportunities for fishery managers who need to make decisions about how to sustainably manage a fishery prior to its initiation. This work proposes and analyzes possible alternative fishery management measures for this potential fishery, under biological and market uncertainty. Due to the lack of knowledge of various aspects of the biology of the resource, the individual growth of the stock was first analyzed and subsequently a bioeconomic analysis of the potential fishery was carried out.

A total of 240 otoliths and standard-length data from 932 specimens sampled from May to December 2015 were used for the analysis of individual growth. The annual formation of otolith increments was verified using the marginal increment analysis and the edge analysis. Age was estimated from annuli counts in sagittal sections of otoliths and growth was analyzed using a multi-model inference approach. The von Bertalanffy growth models (VBGM), generalized VBGM, Gompertz, Logistic and Johnson growth models were considered, which were fitted to observed and back calculated length-at-age data sets grouped by sex. According to Akaike's information criterion, the generalized VBGM was the most appropriate for females (L∞= 31.36 cm, k= 0.15 cm year-1), while the VBGM provided the best fit for males (L∞= 25.35 cm, k= 0.28 cm year-1).

The bioeconomic analysis of management alternatives for a potential dwarf hake fishery indicated that a new fishery for said stock could be biologically sustainable and economically profitable, under a set of management strategies and control rules. Limited access with low fishing effort is recommended as it is more profitable by boat and biologically precautionary, considering the high uncertainty associated with the exploitation of an unfished stock. Despite the combination of high fishing costs and low prices, the fishery could still be profitable in the long term, although there is risk of overexploitation if high fishing effort is allowed..."

Merluza enana, stock no explotado, crecimiento individual, análisis bioeconómico, pesquería potencial Dwarf hake, unexploited stock, individual growth, bioeconomic analysis, potential fishery CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE REGLAMENTACIÓN Y CONTROL REGLAMENTACIÓN Y CONTROL

Diferenciación gonadal e identificación del período lábil para el control del sexo en la lobina rayada, Morone saxatilis

Gonadal differentiation and identification of the labile period for sex control in striped bass, Morone saxatilis

Estefanía de los Ángeles Murillo Núñez (2024, [Tesis de maestría])

La lobina rayada (Morone saxatilis) es un pez teleósteo perciforme anádromo que pertenece a la familia Moronidae. Esta especie se destaca por sus notables cualidades para la acuicultura. La empresa Pacífico Aquaculture, S. A. P. I. de C. V., agregó a sus cultivos a la lobina rayada, puesto que es de gran valor comercial y tiene una gran demanda en el mercado local, nacional e internacional. Aunque existe un creciente interés en intensificar el cultivo de la lobina rayada con el fin de mejorar las prácticas productivas, hasta el momento se han llevado a cabo relativamente pocos estudios que describan su proceso de gonadogénesis o que identifiquen el período crítico en el ciclo reproductivo de esta especie. Dado su parentesco con la especie Dicentrarchus labrax, en este estudio, utilizamos esta última como modelo para el diseño de experimentos y la descripción del desarrollo gonadal de la lobina rayada. En este trabajo, se presenta el proceso de diferenciación de las gónadas de la lobina rayada durante su etapa larval y juvenil, que abarca desde 0 hasta 300 días después de la eclosión (dde), utilizando técnicas histológicas. A partir de 32 dde (2.03 cm LT) se desarrolló el primordio gonadal indiferenciado con células germinales. La diferenciación ovárica se determinó con la formación de la cavidad ovárica a los 52 dde (4.97 cm LT). Los primeros ovocitos primarios se desarrollaron a los 102 dde (11.99 cm LT). La diferenciación testicular se identificó a los 140 dde (13.75 cm) con la presencia y proliferación de espermatogonias. El periodo lábil de la lobina rayada es entre los 32 y 52 dde. M. saxatilis es más precoz que D. labrax. Este estudio representa el primer intento sistemático y detallado de describir el proceso de diferenciación de la lobina rayada. Los hallazgos de este trabajo serán de gran utilidad al proporcionar información crucial para el desarrollo de estrategias de manipulación reproductiva con el fin de mejorar los rendimientos biológicos de esta especie en el contexto acuícola.

The striped bass (Morone saxatilis) is an anadromous teleost perciform fish belonging to the Moronidae family. This species stands out for its remarkable qualities in aquaculture. Pacífico Aquaculture, S. A. P. I. de C. V., has incorporated striped bass into its aquaculture operations due to its significant commercial value and high demand in the local, national, and international markets. Although there is a growing interest in intensifying striped bass farming to enhance production practices, relatively few studios have been conducted to describe its gonadogenesis process or identify the critical period in the reproductive cycle of this species. Given its relationship with the Dicentrarchus labrax species, in this study, we used the latter as a model for experimental design and the description of striped bass gonadal development. This work presents the process of gonad differentiation in striped bass during its larval and juvenile stages, spanning from 0 to 300 days post hatching (dph), using histological techniques. The undifferentiated gonadal primordium with germ cells developed starting from 32 dph (2.03 cm TL). Ovarian differentiation was determined with the formation of the ovarian cavity at 52 dph (4.97 cm TL). The first primary oocytes developed at 102 dph (11.99 cm TL). Testicular differentiation was identified at 140 dph (13.75 cm) with the presence and proliferation of spermatogonia. The critical period for striped bass is between 32 and 52 dph. M. saxatilis differentiates earlier than D. labrax. This study represents the first systematic and detailed attempt to describe the striped bass differentiation process. The findings of this work will be of great utility by providing crucial information for the development of reproductive manipulation strategies aimed at enhancing the biological yields of this species in the aquacultural context.

Morone saxatilis, diferenciación sexual, análisis histológico, período lábil, gonadogénesis Morone saxatilis, sex differentiation, histological analysis, labile period, gonadogenesis CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE PISCICULTURA PISCICULTURA

Variability of Microcirculation Detected by Blood Pulsation Imaging

Alexei Kamshilin (2013, [Artículo])

The non-invasive assessment of blood flow is invaluable for the diagnostic and monitoring treatment of numerous vascular and neurological diseases. We developed a non-invasive and non-contact method of blood pulsation imaging capable of visualizing and monitoring of the two-dimensional distribution of two key parameters of peripheral blood flow: the blood pulsation amplitude and blood pulsation phase. The method is based on the photoplethysmographic imaging in the reflection mode. In contrast with previous imaging systems we use new algorithm for data processing which allows two dimensional mapping of blood pulsations in large object's areas after every cardiac cycle. In our study we carried out the occlusion test of the arm and found (i) the extensive variability of 2D-distribution of blood pulsation amplitude from one cardiac cycle to another, and (ii) existence of the adjacent spots to which the blood is asynchronously supplied. These observations show that the method can be used for studying of the multicomponent regulation of peripheral blood circulation. The proposed technique is technologically simple and cost-effective, which makes it applicable for monitoring the peripheral microcirculation in clinical settings for example, in diagnostics or testing the efficiency of new medicines. © 2013 Kamshilin et al.

algorithm, article, blood analysis, heart cycle, human, human experiment, image analysis, imaging system, male, microcirculation, peripheral circulation, photoelectric plethysmography, process development, Algorithms, Diagnostic Imaging, Diagnostic T CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA MATEMÁTICAS ANÁLISIS NUMÉRICO ANÁLISIS NUMÉRICO

Estudios de genética en poblaciones de abulón y sus aplicaciones en ordenamiento pesquero

RICARDO PEREZ ENRIQUEZ NOE DIAZ VILORIA JOSE LUIS GUTIERREZ GONZALEZ ALEJANDRA ARCINIEGA DE LOS SANTOS ADRIANA MAX AGUILAR Pedro Cruz Hernández Fernando Aranceta Garza (2016, [Artículo])

"Se presenta la integración de más de 10 años de investigación científica en genética de las poblaciones de abulón en México realizada en el CIBNOR. Esta investigación muestra cómo se pueden aplicar los marcadores genéticos tanto en estudios de genética poblacional como en identificación forense con la finalidad de contribuir al conocimiento aplicado para manejo de la pesquería. Se desarrollaron marcadores genéticos tipo microsatélites de ADN enfocados tanto al abulón azul Haliotis fulgens como amarillo Haliotis corrugata para diferenciación de poblaciones y análisis de parentesco. Un análisis de estructura genética de las poblaciones silvestres de ambas especies de abulón mostró homogeneidad genética en la costa del Pacífico en la región centro-sur de la Península de Baja California, México, pero con diferenciación genética en localidades distantes debido a un flujo genético limitado producto del aislamiento reproductivo. Por ello, no existen elementos que den soporte a un manejo pesquero delimitado por bancos en ambas especies. De manera particular, el abulón amarillo mostró una menor de diversidad genética que el azul, posiblemente debido a una mayor explotación pesquera histórica. Los resultados obtenidos en pruebas de parentesco han indicado que la retención larvaria en bancos específicos es reducida, por lo que ni la agregación de reproductores ni la liberación de larvas han mostrado ser estrategias eficientes para favorecer el incremento de reclutas en bancos definidos. Un análisis de perfiles genéticos con el gen de la lisina permitió la identificación de las especies de abulón que se capturan y enlatan en México. El análisis comparativo de perfi les genéticos, basado en el gen nuclear 18S de abulón y otros moluscos, detectó producto enlatado conteniendo especies de moluscos comercializadas falsamente como abulón, lo que puede constituirse como una herramienta forense en futuras disputas legales. Este tipo de aplicación es potencialmente utilizable con otros productos comestibles en los cuales se sospecha de prácticas fraudulentas, ya sea por captura o comercialización ilegal o por sustitución de contenidos en productos procesados."

"This is an integrative work of more than 10 years of research in population genetics of abalone in Mexico performed at CIBNOR. It shows how molecular tools have the potential to support abalone fisheries management through population genetics and forensic analyses. Microsatellite DNA markers were developed on blue (green for its name in English) Haliotis fulgens and yellow abalone (pink for its name in English) H. corrugata to be used for genetic differentiation on populations and for parentage analysis. The analysis of genetic structure on wild populations of both species revealed genetic homogeneity in the Pacific coast of the central region of the Baja California Peninsula, Mexico, with genetic differentiation on distant localities due to a limited gene flow as a result of reproduction isolation. From this result we suggest that no evidences were found supporting the management of the fishery based on individual abalone beds. Pink abalone shows lower genetic diversity than green abalone, possibly due to higher historical fishery exploitation. The parentage analysis suggested that larval retention within beds is reduced, indicating that neither broodstockaggregation nor the release of abalone larvae for stock enhancement are efficient strategies to increase recruitment in specific beds.

An analysis of the genetic profiles with the lysine gene allowed the identification of abalone species captured and processed in Mexico. The comparative analysis, based on the 18S gene, among abalone and other mollusks, detected canned product containing mollusks that are commercialized allegedly as abalone or ‘abalone type’, which could constitute a forensic tool in future legal disputes. This type of application can also be used with other edible products in which fraudulent practices are suspected either because of illegal catch or commercialization or substitution in processed products."

Análisis forense, diversidad genética, gen 18S, genética de poblaciones, marcadores genéticos, retención larvaria Forensic analysis, genetic diversity, 18S gene, genetic markers, population genetics, larval retention BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA DE POBLACIONES GENÉTICA DE POBLACIONES

Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis

ELIANA VALENCIA LOZANO LISSET HERRERA ISIDRON Osiel Salvador Recoder-Meléndez Aarón Barraza Celis JOSE LUIS CABRERA PONCE (2022, [Artículo])

"Potato microtuber (MT) development through in vitro techniques are ideal propagules for producing high quality potato plants. MT formation is influenced by several factors, i.e., photoperiod, sucrose, hormones, and osmotic stress. We have previously developed a protocol of MT induction in medium with sucrose (8% w/v), gelrite (6g/L), and 2iP as cytokinin under darkness. To understand the molecular mechanisms involved, we performed a transcriptome-wide analysis. Here we show that 1715 up- and 1624 down-regulated genes were involved in this biological process. Through the protein–protein interaction (PPI) network analyses performed in the STRING database (v11.5), we found 299 genes tightly associated in 14 clusters. Two major clusters of up-regulated proteins fundamental for life growth and development were found: 29 ribosomal proteins (RPs) interacting with 6 PEBP family members and 117 cell cycle (CC) proteins. The PPI network of up-regulated transcription factors (TFs) revealed that at least six TFs–MYB43, TSF, bZIP27, bZIP43, HAT4 and WOX9–may be involved during MTs development. The PPI network of down-regulated genes revealed a cluster of 83 proteins involved in light and photosynthesis, 110 in response to hormone, 74 in hormone mediate signaling pathway and 22 related to aging."

transcriptome-wide analysis, microtubers, potato, Solanum tuberosum, darkness, cell cycle, ribosomal proteins, PEBP family genes, cytokinin BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Dual function of EDTA with silver nanoparticles for root canal treatment–A novel modification

JUAN MANUEL MARTINEZ ANDRADE (2018, [Artículo])

The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ?-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 ?g/ ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT. © 2018 Martinez-Andrade et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

edetic acid, silver nanoparticle, edetic acid, metal nanoparticle, silver, antibacterial activity, antibiotic sensitivity, antifungal activity, antimicrobial activity, Article, atomic absorption spectrometry, atomic force microscopy, biofilm, bovine, BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA

Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

CARLOS ABRAHAM GUERRERO RUIZ (2019, [Artículo])

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA