Efecto del polvo de romero (Rosmarinus officinalis) en el crecimiento, actividad enzimática y composición proximal de juveniles de lobina rayada (Morone saxatilis)

Effect of rosemary powder (Rosmarinus officinalis) on growth, enzyme activity, and proximal composition of juvenile stripped bass (Morone saxatilis)

Erick Adolfo Barrios García (2023, [Tesis de maestría])

La acuicultura intensiva ha experimentado un rápido crecimiento en las últimas décadas. Sin embargo, la intensificación de los cultivos acuícolas ha ocasionado problemas como el estrés en los organismos, desarrollo de enfermedades y un menor crecimiento. Se ha explorado el uso de plantas aromáticas como el romero (Rosmarinus officinalis) por su efecto como antibiótico, antiestresante y estimulante de la secreción de enzimas digestivas y el crecimiento. El objetivo de este estudio fue evaluar la adición de polvo de romero en la dieta de la lobina rayada (Morone saxatilis) y determinar su efecto en la supervivencia, crecimiento, actividad enzimática (tripsina, quimiotripsina, lipasa, amilasa y proteasas alcalinas totales -PAT-) y composición proximal. Se realizó un bioensayo durante 70 días en un sistema de recirculación de agua de mar donde se probaron cuatro dietas experimentales con diferentes cantidades de polvo de romero: control (TC), 2.5 (T2.5), 5 (T5) y 10 (T10) g kg-1 de dieta. Cada tratamiento se realizó por triplicado con 20 peces por réplica con un peso inicial de 19.59 ± 2.43 g. Las lobinas se alimentaron tres veces al día al 4% de su biomasa. Al final del bioensayo, el peso final de los peces en T5 y T10 fue significativamente menor en comparación con el TC. No se observaron diferencias significativas en parámetros de crecimiento y eficiencia alimenticia. El índice hepatosomático en el T10 fue significativamente mayor en relación con TC. En el intestino, la actividad de la tripsina fue significativamente menor en el T10 y las PAT en T2.5 y T10. En ciegos pilóricos, la actividad de la tripsina fue significativamente menor en el T10, la lipasa en T2.5, T5 y T10 y las PAT en T2.5 y T10. La inclusión de polvo de romero en la dieta de lobina en concentraciones de 5 y 10 g kg-1 disminuye el crecimiento y la actividad de tripsina, PAT y lipasa en intestino y ciegos pilóricos. Se requiere investigar la composición química del romero y su efecto como inhibidor de enzimas digestivas y modulador de la microbiota intestinal, además de incluir otros indicadores para conocer de forma integrada el desempeño fisiológico de la especie.

Intensive aquaculture has experienced a very rapid growth in recent years. However, the intensification of aquaculture has led to issues such as stress in organisms, the development of diseases, and reduced growth. The use of aromatic plants, such as rosemary (Rosmarinus officinalis), has been explored due to its antibiotic, anti-stress, and digestive enzyme secretion-stimulating effects, as well as its growth-promoting properties. The objective of this study was to evaluate the addition of rosemary powder to the diet of striped bass (Morone saxatilis) and determine its effect on survival, growth, enzyme activity (trypsin, chymotrypsin, lipase, amylase, and total alkaline proteases -PAT-), and proximate composition. A 70-day bioassay was conducted in a recirculating seawater system, testing four experimental diets with varying amounts of rosemary powder: control (TC), 2.5 (T2.5), 5 (T5), and 10 (T10) g kg-1 of diet. Each treatment was performed in triplicate with 20 fish per replicate, with an initial weight of 19.59 ± 2.43 g. The striped bass were fed three times a day at 4% of their biomass. At the end of the bioassay, the final weight of fish in T5 and T10 was significantly lower compared to TC. There were no significant differences in growth parameters and feed efficiency. The hepatosomatic index in T10 was significantly higher compared to TC. In the intestine, trypsin activity was significantly lower in T10, and PAT in T2.5 and T10. In the pyloric caeca, trypsin activity was significantly lower in T10, lipase in T2.5, T5, and T10, and PAT in T2.5 and T10. The inclusion of rosemary powder in striped bass diets at concentrations of 5 and 10 g kg-1 reduces growth and the activity of trypsin, PAT, and lipase in the intestine and pyloric caeca. Further research is imperative to delve into the intricate chemical composition of rosemary, explore its role as a potent digestive enzyme inhibitor, and its potential as a modulator of the intestinal microbiota. This endeavor should also encompass the incorporation of various additional indicators, aiming for a holistic grasp of the species' physiological performance.

lobina rayada, romero, crecimiento, enzimas digestivas, composición proximal striped bass, rosemary, growth, digestive enzymes, proximal composition CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA ACUICULTURA MARINA OCEANOGRAFÍA ACUICULTURA MARINA

Presencia de Perkinsus marinus en el ambiente y en los moluscos Anadara tuberculosa, Mytella strigata y Crassostrea corteziensis de la costa de Nayarit

Presence of Perkinsus marinus in the environment and in the mollusks Anadara tuberculosa, Mytella strigata and Crassostrea corteziensis from the coast of Nayarit

Naoki Abraham Kawamoto Camacho (2023, [Tesis de maestría])

El parásito Perkinsus marinus cuyo ciclo de vida le permite subsistir en el ambiente y cumplir su ciclo dentro del hospedero es el agente causal de la perkinsosis, letal para Crasssotrea virginica y enlistado por la Organización Mundial de Salud Animal (OMSA). El comercio de ostiones entre el Golfo de México y el Pacífico mexicano ha generado preocupaciones sanitarias al introducir estos moluscos en comunidades costeras del Pacífico. P. marinus formaría parte de la microbiota ambiental y estar en los moluscos bivalvos de estos ambientes. En el presente estudio se emplearon las técnicas PCR, qPCR, secuenciación de amplicones y de próxima generación, así como análisis de microbioma para determinar la presencia de P. marinus en el ambiente y en los moluscos Anadara tuberculosa, Mytella strigata y Crassostrea corteziensis que coexisten en el estero Pozo Chino, Nayarit. Además, se analizaron muestras embebidas en parafina de Tabasco, Veracruz (C. virginica), Nayarit y Sinaloa (C. corteziensis) para realizar comparaciones genotípicas. La prevalencia de infección mediante PCR fue de 86% en ostiones de la zona baja y media del estero, y de 66% en la zona alta, mientras que para M. strigata fue de 2.2% por qPCR. No se detectó a P. marinus en el ambiente ni en A. tuberculosa. La identidad del parásito fue de 86.1 al 100% mediante secuenciación dirigida. Los primers que amplifican productos de 130 pb fueron más eficaces. La no detección del parásito en el entorno se debe a su baja presencia, consecuencia de la baja salinidad, ausencia de infecciones graves y la falta de eventos inusuales de mortalidad, evitando la liberación masiva del parásito en el ambiente. Además, la baja detección en M. strigata y ausencia en A. tuberculosa puede relacionarse con su susceptibilidad. El análisis del microbioma eucariótico mostró a P. marinus como parte de la microbiota en C. corteziensis. En el ambiente los grupos más abundantes fueron copépodos, moluscos, poliquetos y diatomeas. Se requiere profundizar en su estudio en el ambiente y hospederos a lo largo del año para dimensionar mejor el riesgo sanitario que se enfrenta en una zona donde se ha establecido el parásito.

The parasite Perkinsus marinus, whose life cycle allows it to survive in the environment and complete its cycle within the host, is the causative agent of perkinsosis, lethal to Crassostrea virginica, and listed by the World Organisation for Animal Health (WOAH). The trade of oysters between the Gulf of Mexico and the Mexican Pacific coast has raised sanitary concerns by introducing these mollusks into coastal communities on the Pacific. In areas where detection has occurred, P. marinus would be part of the environmental microbiota (plankton/sediments) and in coexisting bivalve mollusks. In this study, PCR, Real-Time PCR (qPCR), amplicon sequencing and next-generation sequencing techniques, as well as microbiome analysis, were employed to determine the presence of P. marinus in the environment (plankton and sediments) and in the mollusks Anadara tuberculosa, Mytella strigata, and Crassostrea corteziensis coexisting in the Pozo Chino estuary, Nayarit. Paraffin-embedded samples from Tabasco, Veracruz (C. virginica), Nayarit, and Sinaloa (C. corteziensis) were also analyzed for genotypic comparisons. The infection prevalence through PCR was 86% in oysters from the lower and middle zones of the estuary and 66% in the upper zone, while for M. strigata, it was 2.2% by RT-PCR. P. marinus was not detected in the environment or in A. tuberculosa. Directed sequencing revealed a parasite identity of 86.1 to 100%. Primers amplifying 130 bp products were more effective. The lack of detection of the parasite in the environment is due to its low presence, resulting from low salinity, the absence of records of severe infections, and the lack of unusual mortality events, preventing massive release of the parasite into the environment. Additionally, the isolated detection in M. strigata and absence in A. tuberculosa may be related to their susceptibility. Analysis of eukaryotic microbiomes identified P. marinus as part of the microbiota in C. corteziensis. In the environment, the most abundant groups were copepods, mollusks, polychaetes, and diatoms. Further studies in the environment and hosts throughout the year are required to better understand the health risks in an area where the parasite has become established.

Perkinsus marinus, Anadara tuberculosa, Mytella strigata, Crassostrea, protista, Pozo Chino, susceptibilidad Perkinsus marinus, Anadara tuberculosa, Mytella strigata, Crassostrea, protist, Pozo Chino, susceptibility CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA ACUICULTURA MARINA OCEANOGRAFÍA ACUICULTURA MARINA

Los Sistemas de Información Geográfica y la vulnerabilidad social.

SALVADOR VILLERIAS SALINAS (2022, [Capítulo de libro])

En el espacio geográfico concurren diversos procesos naturales y sociales: geológicos, hidrológicos, tecnológicos y provocados por el hombre, que pueden provocar cierto riesgo en la población. En este contexto, la forma de evaluarlo se relaciona con el estudio de la amenaza y la vulnerabilidad. Todo esto se encuentra inmerso en complejos procesos (Hilhorst y Bankoff, 2004) que se insertan en la intersección de la relación entre la sociedad y la naturaleza.

CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO GEOGRAFÍA OTRAS

Evaluación de la calidad del agua mediante el uso de δ15N en materia orgánica particulada de cenotes de zonas agropecuarias en el estado de Yucatán

JOCELYNE DAYANNA FRIAS HERNANDEZ (2023, [Tesis de maestría])

CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO HIDROLOGÍA AGUAS SUBTERRÁNEAS AGUAS SUBTERRÁNEAS

Offshore wind energy climate projection using UPSCALE climate data under the RCP8.5 emission scenario

MARKUS SEBASTIAN GROSS (2016, [Artículo])

In previous work, the authors demonstrated how data from climate simulations can be utilized to estimate regional wind power densities. In particular, it was shown that the quality of wind power densities, estimated from the UPSCALE global dataset in offshore regions of Mexico, compared well with regional high resolution studies. Additionally, a link between surface temperature and moist air density in the estimates was presented. UPSCALE is an acronym for UK on PRACE (the Partnership for Advanced Computing in Europe)-weather-resolving Simulations of Climate for globAL Environmental risk. The UPSCALE experiment was performed in 2012 by NCAS (National Centre for Atmospheric Science)- Climate, at the University of Reading and the UK Met Office Hadley Centre. The study included a 25.6-year, five-member ensemble simulation of the HadGEM3 global atmosphere, at 25km resolution for present climate conditions. The initial conditions for the ensemble runs were taken from consecutive days of a test configuration. In the present paper, the emphasis is placed on the single climate run for a potential future climate scenario in the UPSCALE experiment dataset, using the Representation Concentrations Pathways (RCP) 8.5 climate change scenario. Firstly, some tests were performed to ensure that the results using only one instantiation of the current climate dataset are as robust as possible within the constraints of the available data. In order to achieve this, an artificial time series over a longer sampling period was created. Then, it was shown that these longer time series provided almost the same results than the short ones, thus leading to the argument that the short time series is sufficient to capture the climate. Finally, with the confidence that one instantiation is sufficient, the future climate dataset was analysed to provide, for the first time, a projection of future changes in wind power resources using the UPSCALE dataset. It is hoped that this, in turn, will provide some guidance for wind power developers and policy makers to prepare and adapt for climate change impacts on wind energy production. Although offshore locations around Mexico were used as a case study, the dataset is global and hence the methodology presented can be readily applied at any desired location. © Copyright 2016 Gross, Magar. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reprod

atmosphere, climate change, Europe, Mexico, sampling, time series analysis, university, weather, wind power, climate, risk, theoretical model, wind, Climate, Models, Theoretical, Risk, Wind CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Transición de leña a gas licuado a presión (GLP) en el sur de México, oportunidad para la mitigación del cambio climático en la región menos desarrollada del país

Transition from biomass to LP gas in southern Mexico, an opportunity for climate change mitigation in the least developed region in the country

Elio Guarionex Lagunes Díaz María Eugenia González Rosende Alfredo Ortega Rubio (2015, [Artículo])

"En los estados del sur de México, entre un 25% y un 55% de los hogares dependen de la leña para cocinar, lo cual trae consecuencias en el ambiente, el desarrollo y la salud. No obstante, el conocimiento de estas consecuencias y la migración hacia combustibles modernos ha permanecido relegada de las políticas de desarrollo. En este trabajo, partiendo de una descripción del panorama de uso de leña en el país y su importancia como fuente de energía, se presenta una aproximación para estimar ahorros en emisiones de CO2 logrables por la transición a gas licuado a presión (GLP), los cuales pueden alcanzar 3.14 Mt CO2e, 26% menos que el escenario base. Se finaliza con una discusión de la transición hacia combustibles modernos, las barreras que la impiden y los logros y fallos de la distribución de estufas ahorradoras de leña, la principal iniciativa gubernamental para aliviar el consumo de leña en el país."

"Between 25% and 55% of households in southern Mexico depend on biomass for cooking, which carries serious consequences on the environment, development and health. In spite of the knowledge of these consequences, transition from biomass to modern fuels has remained outside energy and development policies. In the present work, after describing the panorama of fuelwood use in the country and its importance as an energy source, an approach is presented for estimating CO2 savings achievable by transition to pressurized liquefied gas (LP). These savings can reach 3.14 Mt CO2e, 26% less than the baseline scenario. At the end we discuss on the transition to modern fuels in Mexico, the barriers that hinder it and the achievements and failures of the distribution of fuelwood saving cookstoves, as the only and most important governmental initiative to alleviate biomass use, comparing it with other priorities in the government's agenda."

Transición energética, cambio climático, política energética. Energy transition, climate change, energy policy. CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO METEOROLOGÍA CONTAMINACIÓN ATMOSFÉRICA CONTAMINACIÓN ATMOSFÉRICA

High quality diet improves lipid metabolic profile and breeding performance in the blue-footed booby, a long-lived seabird

ERICK GONZALEZ MEDINA (2018, [Artículo])

Understanding the role of diet in the physiological condition of adults during reproduction and hence its effect on reproductive performance is fundamental to understand reproductive strategies in long-lived animals. In birds, little is known about the influence of the quality of food consumed at the beginning of the reproductive period and its short-term effects on reproductive performance. To assess the role of diet in the physiological condition of female blue-footed booby, Sula nebouxii (BFBO), during reproduction we evaluated whether individual differences in diet (assessed by using δ13C and δ15N values of whole blood from female birds and muscle tissue of the principal prey species) prior to egg laying and during incubation influenced their lipid metabolic profile (measured as triglyceride levels and C:N ratio) and their reproductive performance (defined by laying date, clutch size and hatching success). Females with higher δ15N values in their blood during the courtship and incubation periods had a higher lipid metabolic profile, earlier laying date, greater clutch size (2–3 eggs) and higher hatching success. Females that laid earlier and more eggs (2–3 eggs) consumed more Pacific anchoveta (Cetengraulis mysticetus) and Pacific thread herring (Opisthonema libertate) than did other females. These two prey species also had high amounts of lipids (C:N ratio) and caloric content (Kcal/g fresh weight). The quality of food consumed by females at the beginning of reproduction affected their physiological condition, as well as their short-term reproductive performance. Our work emphasizes the importance of determining the influence of food quality during reproduction to understand the reproductive decisions and consequences in long-lived animals. © 2018 González-Medina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

nitrogen 15, triacylglycerol, lipid, animal experiment, Article, breeding, carbon nitrogen ratio, clutch size, controlled study, courtship, diet, egg laying, female, food intake, hatching, lipid metabolism, muscle tissue, nonhuman, prey, reproduction CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Conjuntos de dinoflagelados del Cretácico Inferior de Colombia

Assemblages of dinoflagellate from the Lower Cretaceous of Colombia

Maribel Reyes Tixteco (2023, [Tesis de maestría])

Los dinoflagelados se usan como indicadores en investigaciones ecológicas y son uno de los principales causantes de toxicidad en florecimientos algales nocivos (FANs). Estas microalgas producen quistes resistentes y fosilizables, los cuales son muy útiles como indicadores bioestratigráficos y paleoecológicos. Los dinoflagelados del Cretácico han sido ampliamente estudiados en estratos de latitudes medias y altas, pero los conjuntos tropicales casi no han sido estudiados. Para ayudar a remediar esta falta de conocimiento, se estudiaron cuantitativamente y cualitativamente el contenido de dinoflagelados de 50 muestras de núcleos de los pozos ANH-CR-MONTECARLO-1X y ANH-CON-06-ST-S, localizados en Colombia. La edad de la sedimentación en ambos pozos se interpreta como Albiano, aunque hay un poco de incertidumbre en la edad de inicio. Los taxones heterotróficos tienen menor riqueza de especies, pero presentan mayor abundancia, mientras que los autótrofos tienen mayor riqueza de especies pero menor abundancia. En el pozo ANH-CR-MONTECARLO-1X se identificaron 20 géneros y 24 especies, con altos valores de concentración total de 1,441.00 qst / gr sed, en la muestra IIES-PALY-6015 por la presencia de Subtilisphaera y 1,036.88 en la muestra IIES-PALY-6021 dominada por Muderongia. Dichas muestras son correlacionables con dos transgresiones marinas, del Albiano tardío (104.4 Ma) y el Albiano temprano (111 Ma) respectivamente. Además, indican que los ensamblajes de quistes en este pozo, están dominados por formas Peridinoideas y Ceratioideas. En el pozo ANH-CON-06-ST-S se encontraron 16 géneros y 15 especies, con un valor máximo de 3,539.62 qst / gr sed en la muestra IIES-PALY-5995 por el alto contenido de Subtilisphaera, y correlacionable con la transgresión marina del Albiano tardío (104.4 Ma). Los ensamblajes de quistes en este pozo, están dominados por las formas Peridinoideas. La litología y el contenido de palinomorfos de los pozos indican un ambiente nerítico interno (NI) a transicional (T), con mayor aporte de terrígenos en el pozo ANH-CON-06-ST-S. Se propone un clima cálido para el Cretácico Inferior por la alta diversidad de taxones autótrofos característicos de aguas cálidas y estratificadas.

Dinoflagellates produce resistant and fossilizable cysts, which are useful as biostratigraphic and paleoecological indicators. These microalgae are used as indicators in ecological studies and are one of the main causes of toxicity in harmful algal blooms (HABs). Cretaceous dinoflagellates have been extensively studied in mid- and high-latitude strata, but tropical assemblages have hardly been studied. To help remedy this lack of knowledge, the dinoflagellate content of 50 core samples from the ANH-CR-MONTECARLO-1X and ANH-CON-06-ST-S wells, located in Colombia, were quantitatively and qualitatively studied. The age of sedimentation in both wells is interpreted as Albian, although there is some uncertainty in the beginning of the depositional age. Heterotrophic taxa have lower species richness but higher abundance, while autotrophs have higher species richness but lower abundance. In the ANH-CR-MONTECARLO-1X well, 20 genera and 24 species were identified, with high total concentration values of 1,441.00 cst / gr sed, in the IIES-PALY-6015 sample, due to the presence of Subtilisphaera and 1,036.88 in the IIES sample -PALY-6021, dominated by Muderongia. These samples are correlable with two marine transgressions, from the late Albian (104.4 Ma) and early Albian (111 Ma) respectively. Furthermore, they indicate that the cyst assemblages in this well are dominated by hetrotrophic forms. In the ANH-CON-06-ST-S well, 16 genera and 15 species were found, with a maximum value of 3,539.62 qst / gr sed in the IIES-PALY-5995 sample due to the high content of Subtilisphaera, and correlated with the transgression marina from the late Albian (104.4 Ma). The cyst assemblages in this well are also dominated by the heterotrophic forms. The lithology and palynomorph content of the wells indicate an internal neritic (NI) to transitional (T) environment, with a higher terrigenous influx in the ANH-CON-06-ST-S well. A warm climate is proposed for the Lower Cretaceous due to the high diversity of autotrophic taxa characteristic of warm and stratified waters.

Dinoflagelados, Cretácico Inferior, Bioestratigrafía, Paleoambientes, Paleoecología Dinoflagellates, Lower Cretaceous, Biostratigraphy, Paleoenvironment, Paleoecology CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO GEOLOGÍA GEOLOGÍA REGIONAL GEOLOGÍA REGIONAL

Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture

Sonia Quijano (2020, [Artículo])

Pseudo-nitzschia is a cosmopolitan genus, some species of which can produce domoic acid (DA), a neurotoxin responsible for the Amnesic Shellfish Poisoning (ASP). In this study, we identified P. subpacifica for the first time in Todos Santos Bay and Manzanillo Bay, in the Mexican Pacific using SEM and molecular methods. Isolates from Todos Santos Bay were cultivated under conditions of phosphate sufficiency and deficiency at 16°C and 22°C to evaluate the production of DA. This toxin was detected in the particulate (DAp) and dissolved (DAd) fractions of the cultures during the exponential and stationary phases of growth of the cultures. The highest DA concentration was detected during the exponential phase grown in cells maintained in P-deficient medium at 16°C (1.14 ± 0.08 ng mL-1 DAd and 4.71 ± 1.11 × 10−5 ng cell-1 of DAp). In P-sufficient cultures DA was higher in cells maintained at 16°C (0.25 ± 0.05 ng mL-1 DAd and 9.41 ± 1.23 × 10−7 ng cell-1 of DAp) than in cells cultured at 22°C. Therefore, we confirm that P. subpacifica can produce DA, especially under P-limited conditions that could be associated with extraordinary oceanographic events such as the 2013–2016 "Blob" in the northeastern Pacific Ocean. This event altered local oceanographic conditions and possibly generated the presence of potential harmful species in areas with economic importance on the Mexican Pacific coast. © 2020 Quijano-Scheggia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

domoic acid, domoic acid, kainic acid, Article, cell growth, controlled study, diatom, Mexico, morphology, nonhuman, Pacific Ocean, phylogeny, plant cell, plant growth, Pseudo nitzschia, toxin analysis, cell culture technique, classification, diatom, CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Protein retention assessment of four levels of poultry by-product substitution of fishmeal in rainbow trout (Oncorhynchus mykiss) diets using stable isotopes of nitrogen (δ15N) as natural tracers

DANIEL BADILLO ZAPATA (2014, [Artículo])

This is second part from an experiment where the nitrogen retention of poultry by-product meal (PBM) compared to fishmeal (FM) was evaluated using traditional indices. Here a quantitative method using stable isotope ratios of nitrogen (δ15N values) as natural tracers of nitrogen incorporation into fish biomass is assessed. Juvenile rainbow trout (Oncorhynchus mykiss) were fed for 80 days on isotopically distinct diets in which 0, 33, 66 and 100% of FM as main protein source was replaced by PBM. The diets were isonitrogenous, isolipidic and similar in gross energy content. Fish in all treatments reached isotopic equilibrium by the end of the experiment. Two-source isotope mixing models that incorporated the isotopic composition of FM and PBM as well as that of formulated feeds, empirically derived trophic discrimination factors and the isotopic composition of fish that had reached isotopic equilibrium to the diets were used to obtain a quantitative estimate of the retention of each source of nitrogen. Fish fed the diets with 33 and 66% replacement of FM by PBM retained poultry by-product meal roughly in proportion to its level of inclusion in the diets, whereas no differences were detected in the protein efficiency ratio. Coupled with the similar biomass gain of fishes fed the different diets, our results support the inclusion of PBM as replacement for fishmeal in aquaculture feeds. A re-feeding experiment in which all fish were fed a diet of 100% FM for 28 days indicated isotopic turnover occurred very fast, providing further support for the potential of isotopic ratios as tracers of the retention of specific protein sources into fish tissues. Stable isotope analysis is a useful tool for studies that seek to obtain quantitative estimates of the retention of different protein sources. © 2014 Badillo et al.

nitrogen 15, nitrogen, protein intake, animal behavior, animal experiment, animal food, animal tissue, aquaculture, Article, biomass, controlled study, energy metabolism, food composition, juvenile animal, nonhuman, poultry by product meal, protein a CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA