Research for development approaches in mixed crop-livestock systems of the Ethiopian highlands

Million Gebreyes James Hammond Lulseged Tamene Getachew Agegnehu Rabe Yahaya Anthony Whitbread (2023)

This study presents processes and success stories that emerged from Africa RISING's Research for Development project in the Ethiopian Highlands. The project has tested a combination of participatory tools at multiple levels, with systems thinking and concern for sustainable and diversified livelihoods. Bottom-up approaches guided the selection of technological interventions that could address the priority farming system challenges of the communities, leading to higher uptake levels and increased impact. Joint learning, appropriate technology selection, and the creation of an enabling environment such as the formation of farmer research groups, the establishment of innovation platforms, and capacity development for institutional and technical innovations were key to this study. The study concludes by identifying key lessons that focus more on matching innovations to community needs and geographies, systems orientation/integration of innovations, stepwise approaches to enhance the adoption of innovations, documenting farmers' capacity to modify innovations, building successful partnerships, and facilitating wider scaling of innovations for future implementation of agricultural research for development projects.

Article

Action Research Systems Thinking CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA INNOVATION PARTNERSHIPS SCALING UP INTEGRATED CROP-LIVESTOCK SYSTEMS

Scaling up Biofortification through Seed Policy Reforms

arun joshi (2022)

Conference object

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SCALING UP BIOFORTIFICATION AGRICULTURAL POLICIES REFORMS SEED

The Scaling Scan: Una herramienta práctica para determinar oportunidades y desafíos para escalar innovaciones de manera sostenible

Lennart Woltering (2023)

Book

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA INNOVATION ADOPTION SOCIAL INCLUSION ENVIRONMENTAL FACTORS SCALING UP

Sorghum value chain analysis in semi-arid Zimbabwe

Abbyssinia Mushunje Munyaradzi Junia Mutenje Charles Pfukwa (2019)

Article

Small Scale Farmers Extension Networks CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRO-INDUSTRIAL SECTOR MARKETING MARGINS SORGHUM VALUE CHAINS

Implementing the nature's contributions framework: A case study based on farm typologies in small-scale agroecosystems from the Mexico highlands

Laura Alicia Rodríguez-Bustos natalia palacios rojas (2023)

Article

Small-Scale Agriculture MasAgro CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE POLICIES AGROECOSYSTEMS SMALL SCALE FARMING

The search for cryptic L-Rhamnosyltransferases on the Sporothrix schenckii genome

Hector M. Mora-Montes Karina García-Gutiérrez Laura Cristina García Carnero Nancy Lozoya-Perez Jorge Humberto Ramírez Prado (2022)

The fungal cell wall is an attractive structure to look for new antifungal drug targets and for understanding the host-fungus interaction. Sporothrix schenckii is one of the main causative agents of both human and animal sporotrichosis and currently is the species most studied of the Sporothrix genus. The cell wall of this organism has been previously analyzed, and rhamnoconjugates are signature molecules found on the surface of both mycelia and yeast-like cells. Similar to other reactions where sugars are covalently linked to other sugars, lipids, or proteins, the rhamnosylation process in this organism is expected to involve glycosyltransferases with the ability to transfer rhamnose from a sugar donor to the acceptor molecule, i.e., rhamnosyltransferases. However, no obvious rhamnosyltransferase has thus far been identified within the S. schenckii proteome or genome. Here, using a Hidden Markov Model profile strategy, we found within the S. schenckii genome five putative genes encoding for rhamnosyltransferases. Expression analyses indicated that only two of them, named RHT1 and RHT2, were significantly expressed in yeast-like cells and during interaction with the host. These two genes were heterologously expressed in Escherichia coli, and the purified recombinant proteins showed rhamnosyltransferase activity, dependent on the presence of UDP-rhamnose as a sugar donor. To the best of our knowledge, this is the first report about rhamnosyltransferases in S. schenckii. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Article

FUNGAL CELL-WALL GLYCANS RHAMNOCONJUGATES RHAMNOSYLTRANSFERASE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) ECOLOGÍA VEGETAL ECOLOGÍA VEGETAL

Pertinencia cultural del enfoque de graduación en mujeres en extrema pobreza en México 2019-2021

Benito Salvatierra Laura Itzel Ramírez Ramos Austreberta Nazar_Beutelspacher BARBARA CAROLINA LINARES BRAVO (2023)

La presente obra expone los resultados de la evaluación de cinco indicadores de pertinencia cultural (expectativas, experiencias, estrategias, valores y decisiones) del proyecto “Empoderamiento de Mujeres y Jóvenes a través del Enfoque de Graduación en Inclusión Financiera en México”, instrumentado en Chiapas y Yucatán por la ONG internacional Trickle Up con el apoyo de cuatro organizaciones sociales locales. Este libro cobra relevancia en un contexto hasta este momento, caracterizado por 50 años de trayectoria de políticas públicas y programas sociales que no han logrado disminuir las desigualdades sociales y económicas que afectan principalmente a la población rural e indígena del país, por lo que pone en manos del lector un marco teórico conceptual, metodológico y de postulados sobre la importancia de la pertinencia cultural y la necesidad de integrarla a los proyectos y programas, ya sean de carácter público, social o privada, como una forma de garantizar su apropiación y continuidad por la población local. En ese sentido, el Enfoque de Graduación se presenta como una alternativa viable y factible para combatir la pobreza general y pobreza extrema, en la medida en que su diseño, como la instrumentación de cada uno de sus componentes (focalización, alfabetización financiera, acompañamiento, capacitación, transferencia de activos, protección social y apoyo al consumo) se realice tomando en cuenta las buenas prácticas, los aprendizajes y desde luego, considere reajustes que atiendan las barreras y recomendaciones derivadas de esta evaluación integral. Finalmente, podrán encontrar de forma detallada el proceso de construcción de variables e indicadores, la metodología (cuantitativa y cualitativa) utilizada por el equipo evaluador de ECOSUR, por lo que este libro puede ser una referencia metodológica y estadística para futuras evaluaciones de proyectos que buscan una incidencia socioeconómica en el bienestar de la población.

Book

Organización Trickle Up Las Américas Organizaciones femeninas Pertinencia cultural Mujeres indígenas Programas de financiamiento Empoderamiento Pobreza Organizaciones no gubernamentales Indicadores económicos Yucatán (México) Chiapas (México) CIENCIAS SOCIALES SOCIOLOGÍA CAMBIO Y DESARROLLO SOCIAL DESARROLLO SOCIO-ECONÓMICO DESARROLLO SOCIO-ECONÓMICO

Nitrogen use efficiency and genotype-by-environment interaction in durum wheat genotypes under varying nitrogen supply

Tesfaye Geleta Aga Bekele Abeyo (2024)

Article

Straw Nitrogen Uptake Nitrogen Harvest Index CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA NITROGEN-USE EFFICIENCY STRAW GENOTYPE ENVIRONMENT INTERACTION HARD WHEAT

In vitro Digestibility of Yarrowia lipolytica Yeast and Growth Performance in Whiteleg Shrimp Litopenaeus vannamei

ANA RUTH ALVAREZ SANCHEZ CLAUDIO HUMBERTO MEJIA RUIZ Héctor Gerardo Nolasco Soria Alberto Peña Rodríguez (2018)

"Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding and nutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and high content of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y. lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed onwhiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced optical density of a yeast suspension. Digestion was –0.00236 ± 0.00010 OD U min–1 for intact cells and –0.00325 ± 0.00010 OD U min–1 for lysed cells. Release of reducing sugars in intact cells (5.3940 ± 0.1713 μmol h–1), and lysed cells (0.8396 ± 0.2251 μmol h–1) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control. Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged. Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp."

Article

Marine yeast, cell digestibility, cell viability, turbidimetry, reduced sugars BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOQUÍMICA BIOQUÍMICA DE ALIMENTOS BIOQUÍMICA DE ALIMENTOS

Screening of xylose utilizing and high lipid producing yeast strains as a potential candidate for industrial application

Linnea Qvirist RICARDO VAZQUEZ JUAREZ Thomas Andlid (2022)

"Background: Sustainable production of oil for food, feed, fuels and other lipid-based chemicals is essential to meet the demand of the increasing human population. Consequently, novel and sustainable resources such as lignocel- lulosic hydrolysates and processes involving these must be explored. In this paper we screened for naturally-occurring xylose utilizing oleaginous yeasts as cell factories for lipid production, since pentose sugar catabolism plays a major role in efcient utilization of lignocellulosic feedstocks. Glycerol utilization, which is also benefcial in yeast-based oil production as glycerol is a common by-product of biodiesel production, was investigated as well. Natural yeast isolates were studied for lipid accumulation on a variety of substrates, and the highest lipid accumulating strains were further investigated in shake fask cultivations and fermenter studies on xylose and hydrolysate. Results: By collecting leaves from exotic plants in greenhouses and selective cultivation on xylose, a high frequency of oleaginous yeasts was obtained (>40%). Diferent cultivation conditions lead to diferences in fatty acid contents and compositions, resulting in a set of strains that can be used to select candidate production strains for diferent purposes. In this study, the most prominent strains were identifed as Pseudozyma hubeiensis BOT-O and Rhodosporidium toruloides BOT-A2. The fatty acid levels per cell dry weight after cultivation in a nitrogen limited medium with either glucose, xylose or glycerol as carbon source, respectively, were 46.8, 43.2 and 38.9% for P. hubeiensis BOT-O, and 40.4, 27.3 and 42.1% for BOT-A2. Furthermore, BOT-A2 accumulated 45.1% fatty acids per cell dry weight in a natural plant hydrolysate, and P. hubeiensis BOT-O showed simultaneous glucose and xylose consumption with similar growth rates on both carbon sources. The fatty acid analysis demonstrated both long chain and poly-unsaturated fatty acids, depending on strain and medium. Conclusions: We found various natural yeast isolates with high lipid production capabilities and the ability to grow not only on glucose, but also xylose, glycerol and natural plant hydrolysate. R. toruloides BOT-A2 and P. hubeiensis BOT-O specifcally showed great potential as production strains with high levels of storage lipids and comparable growth to that on glucose on various other substrates, especially compared to currently used lipid production strains..."

Article

Microbial lipids, Oleaginous yeast, Lignocellulose, Pseudozyma hubeiensis, Rhodosporidium toruloides BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA MICROBIOLOGÍA