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The input reduction principle of agroecology is wrong when it comes to mineral fertilizer use in sub-Saharan Africa

Gatien Falconnier Marc Corbeels Frédéric Baudron Antoine Couëdel leonard rusinamhodzi bernard vanlauwe Ken Giller (2023, [Artículo])

Can farmers in sub-Saharan Africa (SSA) boost crop yields and improve food availability without using more mineral fertilizer? This question has been at the center of lively debates among the civil society, policy-makers, and in academic editorials. Proponents of the “yes” answer have put forward the “input reduction” principle of agroecology, i.e. by relying on agrobiodiversity, recycling and better efficiency, agroecological practices such as the use of legumes and manure can increase crop productivity without the need for more mineral fertilizer. We reviewed decades of scientific literature on nutrient balances in SSA, biological nitrogen fixation of tropical legumes, manure production and use in smallholder farming systems, and the environmental impact of mineral fertilizer. Our analyses show that more mineral fertilizer is needed in SSA for five reasons: (i) the starting point in SSA is that agricultural production is “agroecological” by default, that is, very low mineral fertilizer use, widespread mixed crop-livestock systems and large crop diversity including legumes, but leading to poor soil fertility as a result of widespread soil nutrient mining, (ii) the nitrogen needs of crops cannot be adequately met solely through biological nitrogen fixation by legumes and recycling of animal manure, (iii) other nutrients like phosphorus and potassium need to be replaced continuously, (iv) mineral fertilizers, if used appropriately, cause little harm to the environment, and (v) reducing the use of mineral fertilizers would hamper productivity gains and contribute indirectly to agricultural expansion and to deforestation. Yet, the agroecological principles directly related to soil fertility—recycling, efficiency, diversity—remain key in improving soil health and nutrient-use efficiency, and are critical to sustaining crop productivity in the long run. We argue for a nuanced position that acknowledges the critical need for more mineral fertilizers in SSA, in combination with the use of agroecological practices and adequate policy support.

Manure Crop Yields Smallholder Farming Systems Environmental Hazards CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA BIOLOGICAL NITROGEN FIXATION LEGUMES NUTRIENT BALANCE SOIL FERTILITY AGROECOLOGY YIELD INCREASES LITERATURE REVIEWS

Closing the yield gap of soybean (Glycine max (L.) Merril) in Southern Africa: a case of Malawi, Zambia, and Mozambique

Siyabusa Mkuhlani Isaiah Nyagumbo (2023, [Artículo])

Introduction: Smallholder farmers in Sub-Saharan Africa (SSA) are increasingly producing soybean for food, feed, cash, and soil fertility improvement. Yet, the difference between the smallholder farmers’ yield and either the attainable in research fields or the potential from crop models is wide. Reasons for the yield gap include low to nonapplication of appropriate fertilizers and inoculants, late planting, low plant populations, recycling seeds, etc. Methods: Here, we reviewed the literature on the yield gap and the technologies for narrowing it and modelled yields through the right sowing dates and suitable high-yielding varieties in APSIM. Results and Discussion: Results highlighted that between 2010 and 2020 in SSA, soybean production increased; however, it was through an expansion in the cropped area rather than a yield increase per hectare. Also, the actual smallholder farmers’ yield was 3.8, 2.2, and 2.3 times lower than the attainable yield in Malawi, Zambia, and Mozambique, respectively. Through inoculants, soybean yield increased by 23.8%. Coupling this with either 40 kg ha−1 of P or 60 kg ha−1 of K boosted the yields by 89.1% and 26.0%, respectively. Overall, application of 21–30 kg ha-1 of P to soybean in SSA could increase yields by about 48.2%. Furthermore, sowing at the right time increased soybean yield by 300%. Although these technologies enhance soybean yields, they are not fully embraced by smallholder farmers. Hence, refining and bundling them in a digital advisory tool will enhance the availability of the correct information to smallholder farmers at the right time and improve soybean yields per unit area.

Decision Support Tools Digital Tools Site-Specific Recommendations CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA DECISION SUPPORT SYSTEMS LEGUMES YIELDS SOYBEANS

Microbiological Analysis of the Air in a Popular Fish Processing and Marketing Area

Angélica Sinaí Quintanilla Martínez Lizet Aguirre Güitrón Luis Daniel Espinosa Chaurand MAYRA DIAZ RAMIREZ ALEJANDRO DE JESUS CORTES SANCHEZ (2022, [Artículo])

"Fish are marketed as a food and consumed worldwide. During the production of food, contamination by microorganisms is possible through the air, soil, water, surfaces, food handlers, etc. The air does not have a natural microbial composition, but it is a vehicle for the transmission of microorganisms of economic and health interest because they are associated with food spoilage and human diseases. The objective of this study was the microbiological analysis of the air in an area popular for the processing and marketing of fish products in the city of Tepic Nayarit. Using the passive or sedimentation method to collect microorganisms present in the air, the proportion of aerobic mesophile bacteria, coliform bacteria, fungi and yeast was determined at different locations in the fish processing and marketing area for four weeks. The results indicated that the aerobic mesophiles had the highest counts among all the microbial groups analyzed at the twelve different sampling points during the four weeks of the study; their numbers ranged from 2.44 to 2.95 log CFU/m3/h, followed by molds with counts from 1.44 to 2.75 log CFU/m3/h, yeasts with counts from 0.7 to 2.01 log CFU/m3/h and coliforms with counts that ranged from 0.7 to 1.68 log CFU/m3/h. We determined the proportion of the viable microbiological population present in the air at the different sampling points of the study area; several of these sampling points presented values above those recommended by various agencies around the world. Knowledge of the biological hazards transported through the air is important to establish and reduce the risk to the health of occupants and the contamination pathways of processed and marketed fishery products that may be associated with spoilage and foodborne diseases."

food safety, food quality, air pollution, airborne biohazard, environmental monitoring INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE LOS ALIMENTOS HIGIENE DE LOS ALIMENTOS HIGIENE DE LOS ALIMENTOS

How a holobiome perspective could promote intensification, biosecurity and eco-efficiency in the shrimp aquaculture industry

Eric Daniel Gutiérrez Pérez RICARDO VAZQUEZ JUAREZ FRANCISCO JAVIER MAGALLON BARAJAS MIGUEL ANGEL MARTINEZ MERCADO GRISEL ALEJANDRA ESCOBAR ZEPEDA Paola Magallón Servín (2022, [Artículo])

"The aquaculture industry faces many challenges regarding the intensification of shrimp rearing systems. One of these challenges is the release of excessive amounts of nitrogen and phosphorus into coastal areas, causing disruption in nutrient cycling and microbial equilibrium, which are important for coastal productivity. Biosecurity within the shrimp rearing systems can also be compromised by disruption to the nutrient fluxes, and as consequence the microbiome of the system. In certain conditions, these changes could lead to the blooming of potentially pathogenic bacteria. These changes in the external microbiome of the system and the constant fluctuations of nutrients can affect the intestinal microbiome of shrimp, which is involved in the growth and development of the host, affecting nutrient absorption, regulating metabolic processes, synthesising vitamins, modulating the immune response and preventing growth of pathogenic bacteria. It has been suggested that specific changes in the intestinal microbiome of Litopenaeus vannamei may be an avenue through which to overcome some of the problems that this industry faces, in terms of health, growth and waste. Recent research, however, has focussed mainly on changes in the intestinal microbiome. Researchers have overlooked the relevance of other aspects of the system, such as the microbiome from the benthic biofilms; zooplankton, plankton and bacterioplankton; and other sources of microorganisms that can directly affect the microbial status of the intestinal and epiphytic communities, especially in rearing systems that are based on intensification and microbial maturation processes, such as a biofloc system. It is therefore necessary to place holobiome studies into context, including the ‘holobiome of the aquaculture system’ (microbiomes that make up the culture system and their interactions) and not only the intestinal microbiome. Thus, we describe factors that affect the shrimp microbiome, the methodology of study, from sampling to bioinformatic workflows, and introduce the concept of the ‘holobiome of the aquaculture system’ and how this enables us to promote the intensification, biosafety and eco-efficiency of shrimp farming. The holobiome perspective implies a greater investment of resources and time for research, but it will accelerate the development of technology that will benefit the development and sustainability of the aquaculture industry."

litopenaeus vannamei, microbiome, intensification, biofloc, holobiome of aquaculture systems CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PRODUCCIÓN ANIMAL NUTRICIÓN NUTRICIÓN

Genetic analysis of Vibrio parahaemolyticus O3:K6 strains that have been isolated in Mexico since 1998

CARLOS ABRAHAM GUERRERO RUIZ (2017, [Artículo])

Vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. Environmental and clinical toxigenic strains of V. parahaemolyticus that were isolated in Mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the O3:K6 pandemic clone, and their genetic relationship to strains that are related to the pandemic clonal complex (CC3). Pathogenic tdh+ and tdh+/trh+ strains were analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Also, the entire genome of a Mexican O3:K6 strain was sequenced. Most of the strains were tdh/ORF8-positive and corresponded to the O3:K6 serotype. By PFGE and MLST, there was very close genetic relationship between ORF8/O3:K6 strains, and very high genetic diversities from non-pandemic strains. The genetic relationship is very close among O3:K6 strains that were isolated in Mexico and sequences that were available for strains in the CC3, based on the PubMLST database. The whole-genome sequence of CICESE-170 strain had high similarity with that of the reference RIMD 2210633 strain, and harbored 7 pathogenicity islands, including the 4 that denote O3:K6 pandemic strains. These results indicate that pandemic strains that have been isolated in Mexico show very close genetic relationship among them and with those isolated worldwide. © 2017 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial strain, biofouling, controlled study, Crassostrea, food intake, gene sequence, genetic analysis, genetic variability, Japan, Mexican, Mexico, molecular phylogeny, nonhuman, pandemic, pathogenicity island, sea food, serotyping, toxi BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Usando la descomposición de un grafo Halin para el diseño de algoritmos autoestabilizantes

Using Halin graph decomposition for the design of self-stabilizing algorithm

Daniel Uriel Orozco Lomelí (2023, [Tesis de maestría])

Sea G = (V, E) un grafo no dirigido. El problema de encontrar un conjunto independiente fuerte en G, es identificar un conjunto S ⊆ V , tal que dados dos vértices arbitrarios de S, éstos estén separados entre sí por el menos tres aristas. Encontrar un conjunto S de tamaño máximo pertenece a la clase NP-Difícil. Por otro lado, el problema de encontrar un conjunto dominante total en G es identificar un conjunto D ⊆ V , tal que cualquier vértice en V tenga al menos un vecino que pertenezca a D. Encontrar un conjunto D de tamaño mínimo también pertenece a la clase NP-Difícil. En este trabajo de tesis se diseñaron dos algoritmos, uno que resuelve el problema de encontrar un conjunto independiente fuerte maximal y otro que resuelve el problema de encontrar un conjunto dominante total minimal. Estos dos problemas son menos restrictivos que las versiones de optimización descritas al principio de este texto y se sabe que pertenecen a la clase P. Los algoritmos diseñados corren en un sistema distribuido, son autoestabilizantes, son tolerantes a fallas transitorias y funcionan para grafos Halin. Los grafos Halin pertenecen a la clase de grafos 2-outerplanares y tienen la propiedad de que se pueden partir en dos subgrafos muy conocidos, un árbol y un ciclo. Los algoritmos propuestos aprovechan la propiedad anterior para disminuir la complejidad de los mismos. Hasta donde tenemos conocimiento, los algoritmos propuestos, que corren en tiempo lineal en el número de vértices, son los algoritmos más rápidos existentes para los problemas del conjunto independiente fuerte maximal y el conjunto dominante total minimal.

Let G = (V, E) be an undirected graph. The problem of finding a strong stable set in G, is to identify a set S ⊆ V , such that given two arbitrary vertices of S, they are separated from each other by at least three edges. Finding a set S of maximum size belongs to the class NP-Hard. On the other hand, the problem of finding a total dominanting set in G is to identify a set D ⊆ V , such that any vertex in V has at least one neighbor belonging to D. Finding a set D of minimum size also belongs to the class NP-Hard. In this thesis work, two algorithms were designed, one that solves the problem of finding a maximal strong stable set and one that solves the problem of finding a minimal total dominanting set. These two problems are less restrictive than the optimization versions described at the beginning of this text and are known to belong to the P class. The designed algorithms run on a distributed system, are self-stabilizing, are transient fault tolerant, and work for Halin graphs. Halin graphs belong to the 2-outerplanar class of graphs and have the property that they can be split into two well-known subgraphs, a tree and a cycle. The proposed algorithms take advantage of the above property to decrease the complexity of the algorithms. To the best of our knowledge, the proposed algorithms, which run in linear time in the number of vertices, are the fastest existing algorithms for the maximal strong stable set and minimal total dominating set problems.

Grafo Halin, Sistemas Distribuidos, Autoestabilización, Conjunto Independiente Fuerte, Conjunto Dominante Total Halin Graph, Distributed Systems, Self-stabilizing, Strong Stable Set, Total Dominating Set INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE LOS ORDENADORES LENGUAJES ALGORÍTMICOS LENGUAJES ALGORÍTMICOS

Evaluación de la cinética de liberación de compuestos hidrofílicos y lipofílicos a partir de nanopartículas híbridas polímero-lípido

Evaluation of the release kinetics of hydrophilic and lipophilic compounds from polymer-lipid hybrid nanoparticles

Juan Pablo Carmona Almazán (2023, [Tesis de maestría])

En el tratamiento de enfermedades, la administración de dosis múltiples es una estrategia común para mantener la concentración de los fármacos dentro de un margen terapéutico. Sin embargo, la adherencia de los pacientes a este tipo de tratamiento puede ser un desafío, llevando a una administración irregular de dosis. Una alternativa utilizada para abordar este reto son las nanopartículas híbridas polímero/lípido(NPPLs), las cuales, con menos administraciones, tienen el potencial de alcanzar la dosis necesaria en el tratamiento, posibilitando entonces el incremento del apego al tratamiento. En nuestro proyecto, se llevó a cabo la síntesis de nanopartículas de ácido poli láctico-co-glicólico (PLGA) recubiertas de lecitina de soya, por medio de técnicas de nanoprecipitación y autoensamblaje. Además, integramos estas nanopartículas en una matriz polimérica a base de aerogeles de gelatina de manera que estuvieran distribuidas de manera homogénea y concentrada. Nuestro enfoque central radica en entender la cinética de liberación de un compuesto hidrofílico (ácido gálico) y uno lipofílico (quercetina) a partir de este sistema. Logramos sintetizar nanopartículas con un diámetro hidrodinámico de 100 ± 15 nm, 153 ± 33 y149±21 nm, en el caso de las nanopartículas vacías y cargadas con ácido gálico y cargadas con quercetina, respectivamente. La eficiencia de encapsulación del ácido gálico fue del 90 ± 5 % y de la quercetina fue del 70 ± 10 %. Los resultados que obtuvimos muestran que el ácido gálico sigue una cinética del modelo de Korsmeyer-Peppas, con un valor de n = 1.01 y la quercetina una cinética de primer orden. Dado que los compuestos encapsulados tuvieron una liberación más lenta con respecto a los compuestos libres en los aerogeles de gelatina, nuestro trabajo indica que el encapsulamiento en NPPLs de un compuesto bioactivo, independientemente de su naturaleza química, puede ayudar a retrasar su liberación y reducir el número de dosis administradas, en consecuencia, esto pudiera contribuir a incrementar el apego de un paciente al tratamiento.

In the treatment of diseases, the administration of multiple doses is a common strategy to maintain drug concentrations within a therapeutic range. However, patient adherence to this type of treatment can be challenging, resulting in irregular dosing. An alternative approach used to address this challenge involves polymer/lipid hybrid nanoparticles (NPPLs), which have the potential to achieve the necessary drug dose with fewer administrations, thereby increasing treatment adherence. In our project, we synthesized poly(lactic-co-glycolic acid) (PLGA) nanoparticles coated with soy lecithin using nanoprecipitation and self-assembly techniques. These nanoparticles were then integrated into a polymer matrix based on gelatin aerogels to ensure homogeneous and concentrated distribution. Our main focus was to understand the release kinetics of a hydrophilic compound (gallic acid) and a lipophilic one (quercetin) from this system. We successfully synthesized nanoparticles with a hydrodynamic diameter of 100 ± 15 nm, 153 ± 33 nm, and 149 ± 21 nm for empty nanoparticles, gallic acid-loaded, and quercetin-loaded nanoparticles, respectively. The encapsulation efficiency was 90 ± 5 % for gallic acid and 70 ± 10 % for quercetin. The results we obtained indicate that gallic acid follows Korsmeyer-Peppas kinetics with a value of n = 1.01, while quercetin exhibits first-order kinetics. Since the encapsulated compounds showed slower release compared to free compounds in gelatin aerogels, our work suggests that encapsulation in NPPLs with a bioactive compound, regardless of its chemical nature, can help delay its release and reduce the number of doses administered. Consequently, this could contribute to improve patient treatment adherence.

nanopartículas híbridas, cinética de liberación, sistemas poliméricos, PLGA/lecitina, compuestos hidrofílicos y lipofílicos hybrid nanoparticles, release kinetics, polymeric systems, PLGA/lecithin, hydrophilic and lipophilic compounds INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS INGENIERÍA Y TECNOLOGÍA QUÍMICAS ANÁLISIS DE POLÍMEROS ANÁLISIS DE POLÍMEROS

Protein retention assessment of four levels of poultry by-product substitution of fishmeal in rainbow trout (Oncorhynchus mykiss) diets using stable isotopes of nitrogen (δ15N) as natural tracers

DANIEL BADILLO ZAPATA (2014, [Artículo])

This is second part from an experiment where the nitrogen retention of poultry by-product meal (PBM) compared to fishmeal (FM) was evaluated using traditional indices. Here a quantitative method using stable isotope ratios of nitrogen (δ15N values) as natural tracers of nitrogen incorporation into fish biomass is assessed. Juvenile rainbow trout (Oncorhynchus mykiss) were fed for 80 days on isotopically distinct diets in which 0, 33, 66 and 100% of FM as main protein source was replaced by PBM. The diets were isonitrogenous, isolipidic and similar in gross energy content. Fish in all treatments reached isotopic equilibrium by the end of the experiment. Two-source isotope mixing models that incorporated the isotopic composition of FM and PBM as well as that of formulated feeds, empirically derived trophic discrimination factors and the isotopic composition of fish that had reached isotopic equilibrium to the diets were used to obtain a quantitative estimate of the retention of each source of nitrogen. Fish fed the diets with 33 and 66% replacement of FM by PBM retained poultry by-product meal roughly in proportion to its level of inclusion in the diets, whereas no differences were detected in the protein efficiency ratio. Coupled with the similar biomass gain of fishes fed the different diets, our results support the inclusion of PBM as replacement for fishmeal in aquaculture feeds. A re-feeding experiment in which all fish were fed a diet of 100% FM for 28 days indicated isotopic turnover occurred very fast, providing further support for the potential of isotopic ratios as tracers of the retention of specific protein sources into fish tissues. Stable isotope analysis is a useful tool for studies that seek to obtain quantitative estimates of the retention of different protein sources. © 2014 Badillo et al.

nitrogen 15, nitrogen, protein intake, animal behavior, animal experiment, animal food, animal tissue, aquaculture, Article, biomass, controlled study, energy metabolism, food composition, juvenile animal, nonhuman, poultry by product meal, protein a CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA