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Cytotoxicity of the β- carboline alkaloids harmine and harmaline in human cell assays in vitro01.

JUDITH JIMENEZ GUZMAN LETICIA RIVERON NEGRETE FIKRAT ABDULLAEV JAFAROVA JESUS JAVIER ESPINOSA AGUIRRE ROSARIO RODRIGUEZ ARNAIZ (2008)

β-Carboline alkaloids are natural products widely distributed in plants and also found in alcoholic beverages, well-cooked foods and tobacco smoke. Various authors have reported genotoxic activities of several carboline in prokaryotic and eukaryotic cells that have been attributed to their abilities to intercalate into DNA. But studies on the genotoxic and on the cytotoxic potencies in human cells in vitro are not found in the literature. In the present study the toxicities of one full aromatic β-carboline alkaloid (harmine) and one dihydro-β-carboline alkaloid (harmaline) were evaluated by means of two in vitro human cell assays: the cytochalasin-B blocked micronucleus (CBMN) assay and the viability/colony formation assay with four different human cultured non-transformed (CCD18Lu) and transformed (HeLa, C33A and SW480) cells. Neither alkaloid was able to induce micronuclei levels above that of control levels in a wide range of doses tested; although, harmine at the highest concentrations assayed induced apoptotic as well as necrotic cells. Harmine produced a good viability of all cell lines assayed (control and tumor) while harmaline significantly reduced the viability of transformed and non-transformed cell lines in a dose-dependent manner. Harmine displayed a dose-dependent inhibitory effect on cell proliferation against all human carcinoma cells, but the SW480 transformed cell line showed a higher sensitivity. These results suggested that harmine was identified as a useful inhibitor of tumor development. © 2008 Elsevier GmbH. All rights reserved.

Article

MEDICINA Y CIENCIAS DE LA SALUD Muerte celular - Efectos de drogas Línea celular tumoral Proliferación de células - Efectos de drogas Estimulantes del sistema nervioso central - Toxicidad Harmalina - Toxicidad Cell death - Drug effects Cell line, tumor Cell proliferation - Drug effects Central nervous system stimulants - Toxicity Harmaline - Toxicity Harmina - Toxicidad Harmine - Toxicity Β-carbolina alcaloides Citocalasina B bloqueado ensayo de micronúcleos (CBMN) Viabilidad y ensayo de formación de colonia de células β-Carboline alkaloids Harmaline Cytochalasin-B blocked micronucleus (CBMN) assay Viability and cell colony formation assay

Metabolic activation of herbicide products by Vicia faba detected in human peripheral lymphocytes using alkaline single cell gel electrophoresis.

MARIA ELENA CALDERON SEGURA BERTHA MOLINA ALVAREZ (2007)

Ametryn and metribuzin S-triazines derivatives and EPTC thiocarbamate are herbicides used extensively in Mexican agriculture, for

example in crops such as corn, sugar cane, tomato, wheat, and beans. The present study evaluated the DNA damage and cytotoxic effects

of three herbicides after metabolism by Vicia faba roots in human peripheral lymphocytes using akaline single cell gel electrophoresis.

Three parameters were scored as indicators of DNA damage: tail length, percentage of cells with DNA damage (with comet), and level

DNA damage. The lymphocytes were treated for 2 h with 0.5–5.0 lg/ml ametryn or metribuzin and 1.5–10 lg/ml EPTC. Lymphocytes

also were coincubated for 2 h with 20 ll V. faba roots extracts that had been treated for 4 h with 50–500 mg/l of the two triazines or with

the thiocarbamate herbicide or with ethanol (3600 mg/l), as positive control. The lymphocytes treated with three pesticides without in vivo

metabolic activation by V. faba root did not show significant differences in the mean values bewteen genotoxic parameters compared with

negative control. But when human cells were exposed to three herbicides after they had been metabolized the frequency of cell comet, tail

length and level DNA damage all increased. At highest concentrations of the three herbicides produced severe DNA damage compared

with S10 fraction and negative control. The linear regression analysis of the tail length values of three herbicides indicated that there was

genotoxic effect concentration-response relationship with ametryn and ametribuzin but no EPTC. The ethanol induced major increase

DNA damage compared with S10 fraction and the three pesticides. There were not effects in cell viability with treatment EPTC and metribuzin

wherther or not it had been metabolized. High concentrations of ametryn alone and after it had been metabolized decreased cell viability

compared with the negative control. The results demonstrated that the three herbicides needed to be activated by the V. faba root

metabolism to produce DNA damage in human peripheral lymphocyte. The alkaline comet technique is a rapid and sensitive assay, to

quickly evaluate DNA damage the metabolic activation of herbicide products by V. faba root in human cells in vitro.

Article

MEDICINA Y CIENCIAS DE LA SALUD Supervivencia celular - Efectos de drogas Herbicidas - Metabolismo Herbicidas - Toxicidad Linfocitos - Efectos de drogasas Linfocitos - Fisiología Mutágenos - Metabolismo Mutágenos - Toxicidad Extractos vegetales - Farmacología Raíces de plantas - Metabolismo Tiocarbamatos - Toxicidad Triazinas - Metabolismo Triazinas - Toxicidad Vicia faba - Metabolismo Cell Survival - Drug effects Herbicides - Metabolism Herbicides - Toxicity Triazines - Metabolism Triazines - Toxicity Lymphocytes drug effects Lymphocytes - Physiology Mutagens - Metabolism Mutagens - Toxicity Plant extracts - Pharmacology Plant roots - Metabolism Thiocarbamates - Toxicity Vicia faba - Metabolism Ametryn Metribuzin EPTC herbicides Human peripheral lymphocytes In vivo vicia faba metabolic activation Comet tail length DNA damaged cells

Anabolic androgens restore mating after sexual satiety in male rats.

BRYAN VICTOR PHILLIPS FARFAN MONICA DEL CARMEN ROMANO TORRES JOSE ALONSO FERNANDEZ GUASTI (2008)

Los receptores androgénicos y los receptores de estrógenos participan de manera importante en el control neuroendocrino del comportamiento de apareamiento masculino. La saciedad sexual es la inhibición a largo plazo de la conducta masculina de apareamiento después de repetidas eyaculaciones y está asociada a cambios tanto en el receptor de andrógenos como en la expresión de receptores alfa de estrógenos. La expresión de los receptores de andrógenos está regulada por la administración sistémica crónica de andrógenos anabólicos, 5α-dihidrotestosterona o benzoato de estradiol. Este estudio se realizó para investigar el efecto de estos tratamientos sobre el desarrollo y recuperación de la saciedad sexual; Además, también se incluyeron tratamientos de flutamida o tamoxifeno, solos o junto con andrógenos anabólicos. Se inhibió el tratamiento crónico de 15 días con 5-alfa-dihidrotestosterona (5 mg / kg) o tamoxifeno (15 mg / kg), mientras que el tratamiento con benzoato de estradiol (5 mg / kg) facilitó el comportamiento de apareamiento durante el desarrollo de la saciedad sexual. La proporción de animales que eyacularon 48 h después de la saciedad sexual aumentó después de 17 días de tratamiento con una mezcla de andrógenos anabólicos que contenían 2 mg / kg de propionato de testosterona, 2 mg / kg de decanoato de nandrolona y 1 mg / kg de boldenona undecylenate. Este efecto sólo fue bloqueado por la administración combinada de flutamida más tamoxifeno. Los datos sugieren que los metabolitos de andrógenos anabólicos se sinergizan para restaurar el comportamiento de apareamiento después de la saciedad sexual.

Androgen receptors and estrogen receptors importantly participate in the neuroendocrine control of masculine mating behavior. Sexual satiety is the long term inhibition of masculine mating behavior after repeated ejaculations and is associated to changes in both androgen receptor and estrogen receptor-alpha expression. Androgen receptor expression is up-regulated by systemic chronic administration of anabolic androgens, 5alpha-dihydrotestosterone or estradiol benzoate. This study was carried out to investigate the effect of these treatments on sexual satiety development and recovery; additionally flutamide or tamoxifen treatments -- alone or together with anabolic androgens -- were also included. Chronic 15-day treatment with 5alpha-dihydrotestosterone (5 mg/kg) or tamoxifen (15 mg/kg) inhibited, whereas estradiol benzoate treatment (5 mg/kg) facilitated, mating behavior during sexual satiety development. The proportion of animals that ejaculated 48 h after sexual satiety was increased after 17-day treatment with a mixture of anabolic androgens containing 2 mg/kg testosterone propionate, 2 mg/kg nandrolone decanoate and 1 mg/kg boldenone undecylenate. This effect was only blocked by the combined administration of flutamide plus tamoxifen. The data suggest that anabolic androgens metabolites synergize to restore mating behavior after sexual satiety.

Article

MEDICINA Y CIENCIAS DE LA SALUD Anabolizantes - Farmacología Andrógenos - Farmacología Eyaculación - Efecto de drogas Ratas wistar Conducta sexual animal - Efectos de drogas Anabolic agents - Pharmacology Androgens - Pharmacology Ejaculation - Drug effects Sexual behavior, animal - Drug effects Andrógenos anabólicos Estradiol 5α - Dihidrotestosterona Flutamida Tamoxifeno Receptor de andrógenos Receptor de estrógenos Saciedad sexual Anabolic androgens Estradiol 5α - Dihydrotestosterone Flutamide Tamoxifen Androgen receptor Estrogen receptor Sexual satiety

Disulfide Bridges in the Mesophilic Triosephosphate Isomerase from Giardia lamblia Are Related to Oligomerization and Activity

HORACIO REYES VIVAS ADELAIDA DIAZ VILCHIS JORGE PEON PERALTA GUILLERMO MENDOZA HERNANDEZ JOSE IGNACIO DE LA MORA DE LA MORA SERGIO ENRIQUEZ FLORES JULIO LENIN DOMINGUEZ RAMIREZ GABRIEL LOPEZ VELAZQUEZ (2007)

Triosephosphate isomerase from the mesophile Giardia lamblia (GlTIM) is the only known TIM with natural disulfide bridges. We previously found that oxidized and reduced thiol states of GlTIM are involved in the interconversion between native dimers and higher oligomeric species, and in the regulation of enzymatic activity. Here, we found that trophozoites and cysts have different oligomeric species of GlTIM and complexes of GlTIM with other proteins. Our data indicate that the internal milieu of G. lamblia is favorable for the formation of disulfide bonds. Enzyme mutants of the three most solvent exposed Cys of GlTIM (C202A, C222A, and C228A) were prepared to ascertain their contribution to oligomerization and activity. The data show that the establishment of a disulfide bridge between two C202 of two dimeric GlTIMs accounts for multimerization. In addition, we found that the establishment of an intramonomeric disulfide bond between C222 and C228 abolishes catalysis. Multimerization and inactivation are both reversed by reducing conditions. The 3D structure of the C202A GlTIM was solved at 2.1 Å resolution, showing that the environment of the C202 is prone to hydrophobic interactions. Molecular dynamics of an in silico model of GlTIM when the intramonomeric disulfide bond is formed, showed that S216 is displaced 4.6 Å from its original position, causing loss of hydrogen bonds with residues of the active-site loop. This suggests that this change perturb the conformational state that aligns the catalytic center with the substrate, inducing enzyme inactivation. © 2006 Elsevier Ltd. All rights reserved.

Article

MEDICINA Y CIENCIAS DE LA SALUD Dimerización Disulfuros - Metabolismo Giardia lamblia - Efectos de drogas Giardia lamblia - Enzimología Proteínas mutantes - Química Proteínas mutantes - Metabolismo Oocistos -Citología Oocistos - Efectos de drogas Oocistos - Enzimología Estructura cuaternaria de proteína - Efectos de drogas Estructura secundaria de proteína - Efectos de drogas Estructura secundaria de proteína - Química Estructura secundaria de proteína - Metabolismo Triosa-Fosfato isomerasa - Química Triosa-Fosfato isomerasa -Metabolismo Trofozoítos - Citología Trofozoítos - Efectos de drogas Trofozoítos -Enzimología Dimerization Disulfides - Metabolism Giardia lamblia - Drug effects Giardia lamblia - Enzymology Mutant proteins - Chemistry Mutant proteins - Metabolism Oocysts - Cytology Oocysts - Drug effects Oocysts - Enzymology Protein structure, quaternary - Drug effects Protein structure, secondary - Drug effects Protein subunits-Chemistry metabolism Protein transport - Drug effects chemistry Triose-Phosphate isomerase - Metabolism Trophozoites - Cytology Trophozoites- Drug effects Trophozoites- Enzymology Triosephosphate Disulfide bonds Glycolysis Dinamic molecular

La citotoxicidad inducida por Casiopeína IIgly a las células HeLa agota los niveles de glutatión reducido y es prevenida por el sulfóxido de dimetilo.

Casiopeína IIgly induced cytotoxicity to HeLa cells depletes the levels of reduced glutathione and is prevented by dimethyl sulfoxide.

FRANCISCO RADAMES ALEMON MEDINA JOSE LUIS MUÑOZ SANCHEZ LENA RUIZ AZUARA MARIA ISABEL GRACIA MORA (2008)

The newly synthesized copper coordination compound Casiopeína IIgly (Cas IIgly) is a promising alternative drug in the treatment of cancer, since it has shown cytotoxicity and genotoxicity in different tumour models. Given its enhanced effects after ascorbic acid-mediated copper reduction, Cas IIgly's activity is thought to be related to oxidative damage. In the present work, oxidized Cas IIgly failed to induce cytosolic oxidative damage in HeLa cells (only 0.9% of the cell population), and in 2.3% of the treated cells when previously reduced, as evaluated through the oxidation of dihydrorhodamine 123 (DHR 123). However, it showed cytotoxicity, since HeLa cells treated with 10-80 microg/mL Cas IIgly proliferated only at 30% of their normal rate, and at 15% when treated with reduced Cas IIgly. This cytotoxicity is strongly abolished in the presence of the hydroxyl scavenger dimethyl sulfoxide. The decrease, from 3994 to 530 nanograms of reduced glutathione (GSH) per million cells after treatment with 80 microg/mL Casiopeína IIgly, indicates that this drug causes the expenditure of this naturally occurring antioxidant. These results altogether suggest that, albeit Cas IIgly induced cytotoxicity is not related to cytosolic DHR 123 oxidation, it may be related to oxidative damage.

Article

MEDICINA Y CIENCIAS DE LA SALUD Antineoplásicos Antineoplásicos - Toxicidad Antioxidantes - Metabolismo Muerte celular - Efecto de drogas Supervivencia celular Citosol - Efectos de drogas Citosol - Ultrastructura Dimetilsulfóxido - Farmacología Colorantes fluorescentes Glutation - Metabolismo Antineoplastic agents - Antagonists & inhibitors Antineoplastic agents - Toxicity Antioxidants - Metabolism Cell death - Drug effects Cell survival - Drug effects Cytosol - Drug effects Cytosol - Ultrastructure Dimethyl sulfoxide - Pharmacology Fluorescent dyes Glutathione - Metabolism LIgly de la casiopeína Daño oxidativo Dihydrorhodamine 123 (DHR 123) Glutatión reducido (GSH) Casiopeína IIgly Oxidative damage Dihydrorhodamine 123 (DHR 123) Reduced glutathione (GSH)

Gonadectomy and progesterone treatment induce protection in murine cysticercosis.

JOSE ANTONIO VARGAS VILLAVICENCIO CARLOS LARRALDE RANGEL JORGE MORALES MONTOR (2006)

The effects of progesterone on castrated mice of both sexes infected with Taenia crassiceps cysticerci were studied. Gonadectomy and treatment with progesterone before infection decreased parasite loads by 100% compared with intact uninfected mice. mRNA levels of IFN gamma and IL-2 (typically associated to Th1-like profiles) were markedly decreased in infected gonadectomized (Gx) mice, whereas progesterone treatment of infected Gx mice did not affected its expression. mRNA levels of IL-4, and IL-10 (typically associated with Th2-like profiles) were reduced by gonadectomy, whereas restitution with progesterone did not affected this pattern in infected Gx progesterone-treated mice. Infection markedly induced expression of progesterone receptor isoform A in splenocytes of Gx mice (5-fold), whereas isoform B had no changes.

Progesterone metabolism to dehydroepiandrosterone (DHEA) in Gx animals was increased 3-fold only in infected progesterone-treated uninfecteds of both sexes, but was not detectable in infected Gx progesterone-treated mice. Conversely, DHEA levels increased 100-fold in infected Gx progesterone-treated mice. However, androgen receptor expression in splenocytes of male mice showed a reduction by gonadectomy, and by infection, whereas in females AR expression showed no changes in the different mouse groups. These results suggest that progesterone, through its metabolism to DHEA, negatively affects the establishment, growth, and reproduction of Taenia crassiceps, by a mechanism that does not implicate a classic genomic pathway involving a nuclear androgen receptor.

Article

MEDICINA Y CIENCIAS DE LA SALUD Adyuvantes inmunológicos - Metabolismo Adyuvantes inmunológicos - Farmacologia Cisticercosis - Parasitología Cisticercosis - Efectos de drogas Deshidroepiandrosterona - Metabolismo Deshidroepiandrosterona - Farmacología Ratones consanguíneos BALB C - Ratones Orquiectomía Progesterona - Administración & dosificación Progesterona - Metabolismo Progesterona - Uso terapéutico Taenia - Efectos de drogas Taenia - Crecimiento & desarrollo Resultado del tratamiento Adjuvants, immunologic - Metabolism Adjuvants, immunologic - Pharmacology Cysticercosis - Immunology Cysticercosis - Parasitology Cysticercus - Drug effects Dehydroepiandrosterone- Metabolism Dehydroepiandrosterone - Pharmacology Mice, inbred BALB C Orchiectomy Ovariectomy Progesterone - Administration & dosage Progesterone - Metabolism Progesterone - Therapeutic use Progestins - Administration & dosage Progestins - Metabolism Progestins - Therapeutic use Taenia - Drug effects Taenia - Growth & development Treatment outcome Cisticercosis Inmunoendocrinos Metabolismo Progesterona Cysticercosis Immunoendocrine Progesterone Taenia

Use of in vitro assays to assess the potential antigenotoxic and cytotoxic effects of saffron. (Crocus sativus L.)

FIKRAT ABDULLAEV JAFAROVA LETICIA RIVERON NEGRETE HERIBERTO CABALLERO ORTEGA JUAN MANUEL HERNANDEZ LOPEZ ELEAZAR ISRAEL PEREZ LOPEZ ROGELIO GREGORIO PEREDA MIRANDA JESUS JAVIER ESPINOSA AGUIRRE (2003)

Saffron is harvested from the dried, dark red stigmas of Crocus sativus L. flowers. It is used as a spice for flavoring and coloring food and as a perfume. It is often used for treating several diseases. We assessed the antimutagenic, comutagenic and cytotoxic effects of saffron and its main ingredients using the Ames/Salmonella test system, two well known mutagens (BP, 2AA), the in vitro colony formation assay and four different cultured human normal (CCD-18Lu) and malignant (HeLa, A-204 and HepG2) cells. When only using the TA98 strain in the Ames/Salmonella test system, saffron showed non-mutagenic, as well as non-antimutagenic activity against BP-induced mutagenicity, and demonstrated a dose-dependent co-mutagenic effect on 2-AA-induced mutagenicity. The saffron component responsible for this unusual comutagenic effect was safranal. In the in vitro colony formation test system, saffron displayed a dose-dependent inhibitory effect only against human malignant cells. All isolated carotenoid ingredients of saffron demonstrated cytotoxic activity against in vitro tumor cells. Saffron crocin derivatives possessed a stronger inhibitory effect on tumor cell colony formation. Overall, these results suggest that saffron itself, as well as its carotenoid components might be used as potential cancer chemopreventive agents. © 2003 Elsevier Ltd. All rights reserved.

Article

MEDICINA Y CIENCIAS DE LA SALUD Antimutagénicos - Toxicidad Supervivencia celular - Efectos de drogas Crocus - Toxicidad Pruebas de mutagenicidad - Métodos Extractos vegetales - Toxicidad Salmonella typhimurium - Genética Células madre - Efectos de drogas Antimutagenic agents - Toxicity Cell Survival - Drug effects Crocus - Toxicity Mutagenicity tests - Methods Plant Extracts - Toxicity Salmonella typhimurium - Genetics Stem Cells - Drug effects Azafrán Crocus sativus Citotoxicidad Mutagenicidad Co-mutagenicidad Saffron Crocus sativus Cytotoxicity Mutagenicity Co-mutagenicity

Effect of dichlorvos on hepatic and pancreatic glucokinase activity and gene expression, and on insulin mRNA levels.

JOSE GUILLERMO ROMERO NAVARRO TERESITA GUADALUPE LOPEZ ACEVES ALBERTO ROJAS OCHOA MARIA CRISTINA REGINA FERNANDEZ MEJIA (2006)

Several studies have shown that organophosphate pesticides affect carbohydrate metabolism and produce hyperglycemia. It has been reported that exposure to the organophosphate pesticide dichlorvos affects glucose homeostasis and decreases liver glycogen content. Glucokinase (EC 2.7.1.1) is a tissue-specific enzyme expressed in liver and in pancreatic beta cells that plays a crucial role in glycogen synthesis and glucose homeostasis. In the present study we analyzed the effect of one or three days of dichlorvos administration [20 mg/kg body weight] on the activity and mRNA levels of hepatic and pancreatic glucokinase as well as on insulin mRNA abundance in the rat. We found that the pesticide affects pancreatic and hepatic glucokinase activity and expression differently. In the liver the pesticide decreased the enzyme activity; on the contrary glucokinase mRNA levels were increased. In contrast, pancreatic glucokinase activity as well as mRNA levels were not affected by the treatment. Insulin mRNA levels were not modified by dichlorvos administration. Our results suggest that the decreased activity of hepatic glucokinase may account for the adverse effects of dichlorvos on glucose metabolism. © 2005 Elsevier Inc. All rights reserved.

Article

MEDICINA Y CIENCIAS DE LA SALUD Diclorvos - Farmacología - Ratas Regulación de la expresión génica - Genética - Ratas Enzimológica -Efectos de drogas - Ratas Glucoquinasa - Metabolismo - Ratas Insecticidas - Farmacología - Ratas Insulina - Biosíntesis - Ratas Dosificación letal mediana - Ratas Hígado - Efectos de drogas - Ratas Hígado - Enzimología - Ratas Páncreas - Efectos de drogas - Ratas Páncreas - Enzimología - Ratas ARN Mensajero - Biosíntesis - Ratas Ratas wistar Dichlorvos - Pharmacology - Rats Gene expression regulation - Genetics - Rats Enzymologic -Drug effects - rats Glucokinase - Metabolism - Rats Insecticides - Pharmacology - Rats Insulin - Biosynthesis - Rats Lethal dose 50 - Rats Liver - Drug effects - Rats Liver - Enzymology - rats Pancreas - Drug effects - Rats Pancreas - Enzymology - Rats RNA, messenger -Biosynthesis - Rats Rats, wistar Organofosforados Plaguicidas Diclorvos Glucoquinasa Insulina - Ratas Organophosphate Pesticides Dichlorvos Glucokinase Insulin - rats

SIRT2 induces the checkpoint kinase BubR1 to increase lifespan

Brian J. North Michael Rosenberg Karthik Jeganathan Angela Hafner SHADAY MICHAN AGUIRRE Jing Dai Darren Baker Yana Cen Lindsay Wu Anthony Sauve Jan van Deursen Anthony Rosenzweig David Sinclair (2014)

Los ratones que sobreexpresan el gen mitótico de quinasa de punto de control BubR1 viven más tiempo, mientras que los ratones hipomórficos para BubR1 (BubR1 H / H ) viven más cortos y muestran signos de envejecimiento acelerado. A medida que los ratones de tipo salvaje envejecen, los niveles de BubR1 disminuyen en muchos tejidos, un proceso que se propone para subyacer el envejecimiento normal y las enfermedades relacionadas con la edad. Comprender por qué BubR1 disminuye con la edad y cómo retrasar este proceso es, por lo tanto, de considerable interés. Las sirtuinas (SIRT1‐7) son una familia de desacetilasas dependientes de NAD + que pueden retrasar las enfermedades relacionadas con la edad. Aquí, mostramos que la pérdida de los niveles de BubR1 con la edad se debe a una disminución en NAD + y la capacidad de SIRT2 para mantener la lisina-668 de BubR1 en un estado desacetilado, que es contrarrestado por la acetiltransferasa CBP. La sobreexpresión de SIRT2 o el tratamiento de ratones con el precursor de NAD + mononucleótido de nicotinamida (NMN) aumenta la abundancia de BubR1 in vivo . La sobreexpresión de SIRT2 en animales BubR1 H / H aumenta la esperanza de vida media, con un mayor efecto en ratones machos. Juntos, estos datos indican que se justifica una mayor exploración del potencial de SIRT2 y NAD + para retrasar las enfermedades del envejecimiento en los mamíferos.

Article

BIOLOGÍA Y QUÍMICA MEDICINA Y CIENCIAS DE LA SALUD Biología celular Sirtuinas Ratas Lisina Envejecimiento Proteína Kinasa Longevidad Cell biology Sirtuins Rats Lysine Aging Protein kinases Longevity

Efecto del jugo de granada (Punica Granatum) sobre patrones conductuales de ingesta en ratas con diabetes inducida

Effect of pomegranate juice (Punica Granatum) on behavioral patterns of intake in rats with induced diabetes

ELIA HERMINIA VALDES MIRAMONTES CARMEN ALEJANDRINA VIRGEN CARRILLO ALMA GABRIELA MARTINEZ MORENO JESSICA ELIZABETH PINEDA LOZANO VERONICA FONSECA BUSTOS (2019)

Actualmente crece la evidencia sobre el tratamiento alternativo de la diabetes mellitus a través de alimentos funcionales como el jugo granada. La mayoría de los estudios se dirigen al efecto fisiológico del jugo cuando es suministrado forzadamente a ratas diabéticas. El objetivo de esta investigación fue evaluar el efecto de la exposición a libre acceso al jugo de granada sobre el consumo de líquidos, consumo de alimento y peso corporal en ratas diabéticas. Dos grupos de seis ratas sanas y seis hiperglucémicas, inducidas con 60 mg/kg de estreptozotocina intraperitoneal, se expusieron durante 21 días a jugo de granada, agua y alimento. Los resultados mostraron diferencias significativas (p< 0.05) en el consumo de líquidos y el peso corporal en ambos grupos. No se encontraron diferencias significativas en el consumo de alimento (p> 0.05). La exposición a libre acceso al jugo de granada evidenció su aceptación y generó diferencias conductuales en la ingesta

Evidence on the alternative treatment of diabetes mellitus is now growing through functional foods such as pomegranate juice. Most studies focus on the physiological effect of juice when administered by gavage to diabetic rats. The objective was to evaluate the effect of free access to pomegranate juice exposure on the consumption of liquids, food intake, and body weight in diabetic rats. Two groups of six healthy rats and six hyperglycemic rats, induced with 60 mg/kg of intraperitoneal streptozotocin, were exposed to pomegranate juice, water, and food for 21 days. The results showed significant differences (p < 0.05) in fluid consumption and body weight in both groups. There were no significant differences in food intake (p > 0.05). The free access exposure to pomegranate juice showed its acceptance and generated behavioral differences in the intake

Article

MEDICINA Y CIENCIAS DE LA SALUD Granada Diabetes mellitus Ratas Pomegranate Diabetes mellitus Rats