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Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

CARLOS ABRAHAM GUERRERO RUIZ (2019, [Artículo])

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Impact of manures and fertilizers on yield and soil properties in a rice-wheat cropping system

Alison Laing Akbar Hossain (2023, [Artículo])

The use of chemical fertilizers under a rice-wheat cropping system (RWCS) has led to the emergence of micronutrient deficiency and decreased crop productivity. Thus, the experiment was conducted with the aim that the use of organic amendments would sustain productivity and improve the soil nutrient status under RWCS. A three-year experiment was conducted with different organic manures i.e. no manure (M0), farmyard manure@15 t ha-1 (M1), poultry manure@6 t ha-1(M2), press mud@15 t ha-1(M3), rice straw compost@6 t ha-1(M4) along with different levels of the recommended dose of fertilizer (RDF) i.e. 0% (F1), 75% (F2 and 100% (F3 in a split-plot design with three replications and plot size of 6 m x 1.2 m. Laboratory-based analysis of different soil as well as plant parameters was done using standard methodologies. The use of manures considerably improved the crop yield, macronutrients viz. nitrogen, phosphorus, potassium and micronutrients such as zinc, iron, manganese and copper, uptake in both the crops because of nutrient release from decomposed organic matter. Additionally, the increase in fertilizer dose increased these parameters. The system productivity was maximum recorded under F3M1 (13,052 kg ha-1) and results were statistically identical with F3M2 and F3M3. The significant upsurge of macro and micro-nutrients in soil and its correlation with yield outcomes was also observed through the combined use of manures as well as fertilizers. This study concluded that the use of 100% RDF integrated with organic manures, particularly farmyard manure would be a beneficial resource for increased crop yield, soil nutrient status and system productivity in RWCS in different regions of India.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ORGANIC FERTILIZERS YIELDS SOIL PROPERTIES RICE WHEAT CROPPING SYSTEMS

Evaluación de un reactor biológico empacado con cubos de poliuretano para la remoción de materia orgánica y nutrientes

TANIA GUTIERREZ MACIAS Petia Mijaylova Nacheva (2017, [Conferencia])

El objetivo de este trabajo fue evaluar el uso de cubos de poliuretano como medio de soporte de la biopelícula en un reactor aerobio el cual operó de manera continua y flujo descendente durante un periodo de 90 días. Se aplicó una carga orgánica de 1.41 gDQO·m-3·d-1 con un tiempo de residencia hidráulica (TRH) de 4 h y se dio seguimiento al proceso determinando los siguientes parámetros: Demanda Química de Oxigeno (DQO), solidos suspendidos totales (SST) y solidos suspendidos volátiles (SSV), nitrógeno total (NT), nitrógeno amoniacal (N-NH4) y fósforo total (PT). Como resultado se observó que los microorganismos presentes en el agua residual, colonizaron el medio de soporte (cubos de poliuretano) en los primeros 30 días de operación del sistema, alcanzando una remoción de DQO de 50 %. En el transcurso del tiempo la remoción de DQO aumentó hasta lograr remociones máximas de 84 %. Las remociones obtenidas de SST y SSV fueron mayores de 83 %. La remoción de nutrientes registró valores de 50% NT, 95 % de N- NH4 y 29 % PT.

Reactores biológicos Materia orgánica INGENIERÍA Y TECNOLOGÍA

Precompoteo de residuos orgánicos y su efecto en la dinámica poblacional de Einsenia foetida

CARLOS MANUEL ACOSTA DURAN OFELIA SOLIS PEREZ OSCAR GABRIEL VILLEGAS TORRES Lina Cardoso (2013, [Artículo])

El compostaje y el vermicompostaje son técnicas que se utilizan para transformar los residuos sólidos orgánicos en abonos orgánicos (composta y vermicomposta, respectivamente) cuyas características físicas, químicas y biológicas inciden directamente en el mejoramiento del suelo y en el crecimiento de las plantas; sin embargo, durante el proceso, se liberan desechos que agreden al ambiente. El sustrato utilizado para alimentar a las lombrices debe pasar por un periodo previo de compostaje, conocido como precompostaje. El vermicompostaje produce un material en el que la mayoría de los nutrimentos se encuentran en mayor disponibilidad para la planta, comparados con el material resultante de un proceso de compostaje convencional. El precompostaje involucra un mayor tiempo y gasto de insumos, lo que puede incrementar el costo de la vermicomposta, por lo que es necesario establecer el tiempo necesario de precompostaje para que los residuos orgánicos puedan emplearse como sustrato en la producción de la lombriz Eisenia spp.

Residuos orgánicos Precompostaje Vermicomposta INGENIERÍA Y TECNOLOGÍA