Nitrogen fertilizer application alters the root endophyte bacterial microbiome in maize plants, but not in the stem or rhizosphere soil

Alejandra Miranda Carrazco Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Plant-associated microorganisms that affect plant development, their composition, and their functionality are determined by the host, soil conditions, and agricultural practices. How agricultural practices affect the rhizosphere microbiome has been well studied, but less is known about how they might affect plant endophytes. In this study, the metagenomic DNA from the rhizosphere and endophyte communities of root and stem of maize plants was extracted and sequenced with the “diversity arrays technology sequencing,” while the bacterial community and functionality (organized by subsystems from general to specific functions) were investigated in crops cultivated with or without tillage and with or without N fertilizer application. Tillage had a small significant effect on the bacterial community in the rhizosphere, but N fertilizer had a highly significant effect on the roots, but not on the rhizosphere or stem. The relative abundance of many bacterial species was significantly different in the roots and stem of fertilized maize plants, but not in the unfertilized ones. The abundance of N cycle genes was affected by N fertilization application, most accentuated in the roots. How these changes in bacterial composition and N genes composition might affect plant development or crop yields has still to be unraveled.

Bacterial Community Structure DArT-Seq Bacterial Community Functionality Genes Involved in N Cycling CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURAL PRACTICES MAIZE RHIZOSPHERE STEMS NITROGEN FERTILIZERS

Thermal and mechanical properties of PLA-based multiscale cellulosic biocomposites

MIGUEL ANGEL RUZ CRUZ Pedro Jesús Herrera Franco Emmanuel Alejandro Flores Johnson MARIA VERONICA MORENO CHULIM LUCIANO MIGUEL GALERA MANZANO Alex Valadez González (2022, [Artículo])

In this work polylactic acid (PLA) based multiscale cellulosic biocomposites were prepared with the aim to evaluate the effect of the incorporation of cellulose nanocrystals (CNCs) on the PLA biocomposites reinforced with cellulose microfibers (MFCs). For this, PLA composite materials reinforced with both MFCs and with a combination of MFCs and CNCs were prepared, while keeping the content of cellulosic reinforcements constant. The thermal and mechanical properties of these multiscale PLA biocomposites were characterized by thermogravimetry (TGA), differential scanning calorimetry (DSC), flexural mechanical and, dynamic mechanical (DMA) tests. Likewise, they were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The results show that the replacement of MFCs by CNCs in the 1–5% range appreciably modifies the thermal and mechanical properties of multiscale compounds. For example, they increase the thermal stability of the materials, modify the PLA crystallization process and play the role of adhesion promoters since the mechanical properties in flexure increase in the order of 40% and the storage modulus increases in the order of 35% at room temperature. Also, the addition of CNCs increases the relaxation temperature of the material from 50 to 60 °C, thereby expanding the temperature range for its use. © 2022 The Author(s)

MULTISCALE BIOCOMPOSITES CELLULOSE MICROFIBER CELLULOSE NANOCRYSTALS HIERARCHICAL STRUCTURE PROPERTIES INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE MATERIALES PROPIEDADES DE LOS MATERIALES PROPIEDADES DE LOS MATERIALES

Influence of conservation agriculture-based production systems on bacterial diversity and soil quality in rice-wheat-greengram cropping system in eastern Indo-Gangetic Plains of India

Anup Das virender kumar Peter Craufurd Andrew Mcdonald Sonam Sherpa (2023, [Artículo])

Introduction: Conservation agriculture (CA) is gaining attention in the South Asia as an environmentally benign and sustainable food production system. The knowledge of the soil bacterial community composition along with other soil properties is essential for evaluating the CA-based management practices for achieving the soil environment sustainability and climate resilience in the rice-wheat-greengram system. The long-term effects of CA-based tillage-cum-crop establishment (TCE) methods on earthworm population, soil parameters as well as microbial diversity have not been well studied. Methods: Seven treatments (or scenarios) were laid down with the various tillage (wet, dry, or zero-tillage), establishment method (direct-or drill-seeding or transplantation) and residue management practices (mixed with the soil or kept on the soil surface). The soil samples were collected after 7 years of experimentation and analyzed for the soil quality and bacterial diversity to examine the effect of tillage-cum-crop establishment methods. Results and Discussion: Earthworm population (3.6 times), soil organic carbon (11.94%), macro (NPK) (14.50–23.57%) and micronutrients (Mn, and Cu) (13.25 and 29.57%) contents were appreciably higher under CA-based TCE methods than tillage-intensive farming practices. Significantly higher number of OTUs (1,192 ± 50) and Chao1 (1415.65 ± 14.34) values were observed in partial CA-based production system (p ≤ 0.05). Forty-two (42) bacterial phyla were identified across the scenarios, and Proteobacteria, Actinobacteria, and Firmicutes were the most dominant in all the scenarios. The CA-based scenarios harbor a high abundance of Proteobacteria (2–13%), whereas the conventional tillage-based scenarios were dominated by the bacterial phyla Acidobacteria and Chloroflexi and found statistically differed among the scenarios (p ≤ 0.05). Composition of the major phyla, i.e., Proteobacteria, Actinobacteria, and Firmicutes were associated differently with either CA or farmers-based tillage management practices. Overall, the present study indicates the importance of CA-based tillage-cum-crop establishment methods in shaping the bacterial diversity, earthworms population, soil organic carbon, and plant nutrient availability, which are crucial for sustainable agricultural production and resilience in agro-ecosystem.

Metagenomics Bacterial Diversity Rice-Wheat-Greengram CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CONSERVATION AGRICULTURE DNA SEQUENCES EARTHWORMS METAGENOMICS SOIL QUALITY AGROECOSYSTEMS

Climate-smart agricultural practices influence the fungal communities and soil properties under major agri-food systems

madhu choudhary ML JAT Parbodh Chander Sharma (2022, [Artículo])

Fungal communities in agricultural soils are assumed to be affected by climate, weather, and anthropogenic activities, and magnitude of their effect depends on the agricultural activities. Therefore, a study was conducted to investigate the impact of the portfolio of management practices on fungal communities and soil physical–chemical properties. The study comprised different climate-smart agriculture (CSA)-based management scenarios (Sc) established on the principles of conservation agriculture (CA), namely, ScI is conventional tillage-based rice–wheat rotation, ScII is partial CA-based rice–wheat–mungbean, ScIII is partial CSA-based rice–wheat–mungbean, ScIV is partial CSA-based maize–wheat–mungbean, and ScV and ScVI are CSA-based scenarios and similar to ScIII and ScIV, respectively, except for fertigation method. All the scenarios were flood irrigated except the ScV and ScVI where water and nitrogen were given through subsurface drip irrigation. Soils of these scenarios were collected from 0 to 15 cm depth and analyzed by Illumina paired-end sequencing of Internal Transcribed Spacer regions (ITS1 and ITS2) for the study of fungal community composition. Analysis of 5 million processed sequences showed a higher Shannon diversity index of 1.47 times and a Simpson index of 1.12 times in maize-based CSA scenarios (ScIV and ScVI) compared with rice-based CSA scenarios (ScIII and ScV). Seven phyla were present in all the scenarios, where Ascomycota was the most abundant phyla and it was followed by Basidiomycota and Zygomycota. Ascomycota was found more abundant in rice-based CSA scenarios as compared to maize-based CSA scenarios. Soil organic carbon and nitrogen were found to be 1.62 and 1.25 times higher in CSA scenarios compared with other scenarios. Bulk density was found highest in farmers' practice (Sc1); however, mean weight diameter and water-stable aggregates were found lowest in ScI. Soil physical, chemical, and biological properties were found better under CSA-based practices, which also increased the wheat grain yield by 12.5% and system yield by 18.8%. These results indicate that bundling/layering of smart agricultural practices over farmers' practices has tremendous effects on soil properties, and hence play an important role in sustaining soil quality/health.

Agriculture Management Fungal Community Diversity Indices Climate-Smart Agricultural Practices CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURE TILLAGE CLIMATE-SMART AGRICULTURE SOIL ORGANIC CARBON

Bacterial communities in the rhizosphere at different growth stages of maize cultivated in soil under conventional and conservation agricultural practices

Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Farmers in Mexico till soil intensively, remove crop residues for fodder and grow maize often in monoculture. Conservation agriculture (CA), including minimal tillage, crop residue retention and crop diversification, is proposed as a more sustainable alternative. In this study, we determined the effect of agricultural practices and the developing maize rhizosphere on soil bacterial communities. Bulk and maize (Zea mays L.) rhizosphere soil under conventional practices (CP) and CA were sampled during the vegetative, flowering and grain filling stage, and 16S rRNA metabarcoding was used to assess bacterial diversity and community structure. The functional diversity was inferred from the bacterial taxa using PICRUSt. Conservation agriculture positively affected taxonomic and functional diversity compared to CP. The agricultural practice was the most important factor in defining the structure of bacterial communities, even more so than rhizosphere and plant growth stage. The rhizosphere enriched fast growing copiotrophic bacteria, such as Rhizobiales, Sphingomonadales, Xanthomonadales, and Burkholderiales, while in the bulk soil of CP other copiotrophs were enriched, e.g., Halomonas and Bacillus. The bacterial community in the maize bulk soil resembled each other more than in the rhizosphere of CA and CP. The bacterial community structure, and taxonomic and functional diversity in the maize rhizosphere changed with maize development and the differences between the bulk soil and the rhizosphere were more accentuated when the plant aged. Although agricultural practices did not alter the effect of the rhizosphere on the soil bacterial communities in the flowering and grain filling stage, they did in the vegetative stage.

Community Assembly Functional Diversity Intensive Agricultural Practices Plant Microbiome CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SUSTAINABLE AGRICULTURE TILLAGE SOIL BACTERIA MAIZE

Farmers’ perspectives as determinants for adoption of conservation agriculture practices in Indo-Gangetic Plains of India

Ajay Kumar Mishra ML JAT (2022, [Artículo])

Understanding the farmer's perspective has traditionally been critical to influencing the adoption and out-scaling of CA-based climate-resilient practices. The objective of this study was to investigate the biophysical, socio-economic, and technical constraints in the adoption of CA by farmers in the Western- and Eastern-IGP, i.e., Karnal, Haryana, and Samastipur, Bihar, respectively. A pre-tested structured questionnaire was administered to 50 households practicing CA in Western- and Eastern-IGP. Smallholder farmers (<2 ha of landholding) in Karnal are 10% and Samastipur 66%. About 46% and 8% of households test soil periodically in Karnal and Samastipur, respectively. Results of PCA suggest economic profitability and soil health as core components from the farmer's motivational perspective in Karnal and Samastipur, respectively. Promotion and scaling up of CA technologies should be targeted per site-specific requirements, emphasizing biophysical resource availability, socio-economic constraints, and future impacts of such technology.

Smallholder Farmers Agents of Change Technology Diffusion Climate-Smart Practices CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SMALLHOLDERS SOCIAL STRUCTURE IRRIGATION MANAGEMENT TECHNOLOGY CLIMATE-SMART AGRICULTURE CONSERVATION AGRICULTURE

Exploring the genetic diversity and population structure of wheat landrace population conserved at ICARDA genebank

Muhammad Massub Tehseen Fatma Aykut Tonk Ahmed Amri Carolina Sansaloni Ezgi Kurtulus Muhammad Salman Mubarik Kumarse Nazari (2022, [Artículo])

Wheat Landraces Genetic Diversity SNP Markers Analysis of Molecular Variance AMOVA CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA BREEDING DISCRIMINANT ANALYSIS GENETIC VARIATION GENETIC DISTANCE GENETIC IMPROVEMENT GENETIC MARKERS HEXAPLOIDY LANDRACES POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM TRITICUM AESTIVUM WHEAT

Autotrophic denitrification of nitrate rich wastewater in fluidized bed reactors using pyrite and elemental sulfur as electron donors

Maria Federica Carboni Simon Mills SONIA LORENA ARRIAGA GARCIA Gavin Collins Umer Zeeshan Ijaz Piet Nicolaas Luc Lens (2022, [Artículo])

"This study compared denitrification performances and microbial communities in fluidized bed reactors (FBRs) carrying out autotrophic denitrification using elemental sulfur (S0) and pyrite (FeS2) as electron donors. The reactors were operated for 220 days with nitrate loading rates varying between 23 and 200 mg N-NO-3 /Lmiddotd and HRT between 48 and 4 h. The highest denitrification rates achieved were 142.2 and 184.4 mg NNO-3 /Lmiddotd in pyrite and sulfur FBRs, respectively. Pyrite-driven denitrification produced less SO2- 4 and no buffer addition was needed to regulate the pH. The sulfur FBR needed instead CaCO3 to maintain the pH neutral and consequentially more sludge was produced (CaSO4 precipitation). The active community of pyrite-based systems was investigated and Azospira sp., Ferruginibacter sp., Rhodococcus sp. and Pseudomonas sp. were the predominant genera, while Thiobacillus sp. and Sulfurovum sp. dominated the active community in the sulfur FBR. However, Thiobacillus sp. became more dominant when operating at elevated nitrogen loading rate. Patterns of diversity and microbial community assembly were assessed and revealed three distinct stages of microbial community succession which corresponded with the operation of a period of high influent nitrate concentration (135 mg N-NO-3 /L). It is proposed that a high degree of functional redundancy in the initial microbial communities may have helped both reactors to respond better to such high influent nitrate concentration."

Pyrite Elemental sulfur Fluidized bed rector Nitrogen removal 16S rRNA Community assembly CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Genetic diversity and population structure of African sorghum (sorghum bicolor L. moench) accessions assessed through single nucleotide polymorphisms markers

Hussein Shimelis Baloua Nébié Chris Ojiewo Abhishek Rathore (2023, [Artículo])

Heterotic Grouping Breeding Population Development Marker-Assisted Cultivar Development CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA POPULATION STRUCTURE GENE FLOW SINGLE NUCLEOTIDE POLYMORPHISMS SORGHUM BICOLOR BREEDING PROGRAMMES

Population genetic structure of the maize weevil, Sitophilus zeamais, in southern Mexico

Michael Jones Martha Willcox (2023, [Artículo])

Maize Weevil Genetic Structure CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURAL WORKERS FILTRATION GENE FLOW MAIZE SINGLE NUCLEOTIDE POLYMORPHISM SITOPHILUS ZEAMAIS CURCULIONIDAE