Low nitrogen narrows down phenotypic diversity in durum wheat

Tesfaye Geleta Aga Bekele Abeyo (2023, [Artículo])

Clusters Durum Wheat Nitrogen Efficiency CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HARD WHEAT GENETIC DIVERGENCE NORMALIZED DIFFERENCE VEGETATION INDEX NITROGEN PRINCIPAL COMPONENT ANALYSIS

CLÚSTERES LUMINISCENTES DE PLATA CONFINADOS EN ZEOLITAS PARA BIOSENSADO

Cecilia García-Guzmán (2021, [Tesis de maestría])

"Se propone un sistema de biosensado basado en la detección de anticuerpos IgG marcados con fluoresceína-5-isotiocianato (FITC) mediante emisión de clústeres de plata sintetizados dentro de zeolitas (FAUX Ag6) impregnadas con diferentes aminas: polietilenimina (PEI) ramificada, tetraetilenepentamina (TEPA) y dietilenetriamina (DETA). Se caracterizaron y compararon estas partículas funcionalizadas y se bioconjugaron con anticuerpos anti IgG. Finalmente se evaluó la detección de anticuerpos IgG-FITC en concentraciones menores a 10 ng/mL. Demostrando así, como prueba de concepto, un sistema de biosensado innovador, sensible y específico que permite la detección del analito en pocos minutos."

Zeolitas Biosensado Clústeres luminiscentes INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA BIOQUÍMICA INGENIERÍA BIOQUÍMICA INGENIERÍA BIOQUÍMICA

Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

CARLOS ABRAHAM GUERRERO RUIZ (2019, [Artículo])

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA