Novel mutant alleles reveal a role of the extra-large G protein in rice grain filling, panicle architecture, plant growth, and disease resistance

Akshaya Biswal Daisuke Urano (2022, [Artículo])

Heterotrimeric G Proteins Extra-Large G Proteins Cas9 OsXLG CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CRISPR RICE PROTEINS PLANT GROWTH DISEASE RESISTANCE

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Performance evaluation and identification of highland quality protein maize hybrids in Ethiopia

Adefris Teklewold (2022, [Artículo])

Quality Protein Conventional Maize CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE PROTEIN QUALITY CROSS-BREEDING HYBRIDS

Standard heterosis estimation for quality protein maize hybrids over best released and commercialized hybrids

Adefris Teklewold (2022, [Artículo])

Grain Yield Quality Protein Maize CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CROSS-BREEDING INBRED LINES HETEROSIS PROTEIN QUALITY HYBRIDS

Comparison of sequencing-based and array-based genotyping platforms for genomic prediction of maize hybrid performance

XUECAI ZHANG Zefeng Yang Yang Xu (2022, [Artículo])

Genotyping by Sequencing Genomic Selection SNP Array Marker Density CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MARKER-ASSISTED SELECTION MAIZE SINGLE NUCLEOTIDE POLYMORPHISM HYBRIDS

Genotyping by sequencing advancements in barley

Naeela Qureshi Mohammad Pourkheirandish (2022, [Artículo])

Genotyping by Sequencing Target Enrichment CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA BARLEY GENOTYPING RESTRICTION ENZYMES SINGLE NUCLEOTIDE POLYMORPHISM

Balancing quality with quantity: a case study of UK bread wheat

Nick Fradgley Keith Gardner Stéphanie M. Swarbreck Alison Bentley (2023, [Artículo])

Grain Protein Content Environmental Sustainability End-Use Quality Modern Bread Baking Methods CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GRAIN PROTEIN CONTENT HISTORY QUALITY WHEAT YIELDS

Multi-omic characterization of the maize GPI synthesis mutant gwt1 with defects in kernel development

Sarah Hearne zhiyuan fu (2023, [Artículo])

Maize Endosperm Development Membrane Proteomics Glycosyl-Phosphatidyl-Inositol Membrane Anchored Proteins CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE ENDOSPERM MEMBRANES PROTEOMICS TRANSCRIPTOMICS

Determining factors of durum wheat bread loaf volume and alveograph characteristics under optimal, drought and heat stress conditions

Maryke Labuschagne Carlos Guzman Jose Crossa Angeline van Biljon (2023, [Artículo])

Loaf Volume Durum Wheat Flour Protein Content CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ALVEOGRAPHS HARD WHEAT HEAT STRESS DROUGHT STRESS

Coronin is a component of the endocytic collar of hyphae of neurospora crassa and is necessary for normal growth and morphogenesis

RAMON OSVALDO ECHAURI ESPINOSA (2012, [Artículo])

Coronin plays a major role in the organization and dynamics of actin in yeast. To investigate the role of coronin in a filamentous fungus (Neurospora crassa), we examined its subcellular localization using fluorescent proteins and the phenotypic consequences of coronin gene (crn-1) deletion in hyphal morphogenesis, Spitzenkörper behavior and endocytosis. Coronin-GFP was localized in patches, forming a subapical collar near the hyphal apex; significantly, it was absent from the apex. The subapical patches of coronin colocalized with fimbrin, Arp2/3 complex, and actin, altogether comprising the endocytic collar. Deletion of crn-1 resulted in reduced hyphal growth rates, distorted hyphal morphology, uneven wall thickness, and delayed establishment of polarity during germination; it also affected growth directionality and increased branching. The Spitzenkörper of Δcrn-1 mutant was unstable; it appeared and disappeared intermittently giving rise to periods of hyphoid-like and isotropic growth respectively. Uptake of FM4-64 in Δcrn-1 mutant indicated a partial disruption in endocytosis. These observations underscore coronin as an important component of F-actin remodeling in N. crassa. Although coronin is not essential in this fungus, its deletion influenced negatively the operation of the actin cytoskeleton involved in the orderly deployment of the apical growth apparatus, thus preventing normal hyphal growth and morphogenesis. © 2012 Echauri-Espinosa et al.

actin related protein 2-3 complex, F actin, fimbrin protein, fluorescent dye, fungal protein, fungal protein coronin, green fluorescent protein, unclassified drug, actin binding protein, coronin proteins, fungal protein, article, cell polarity, contr BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA