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Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Corrección de defectos óseos en el área de Ingeniería tisular

Correction of bone defects by tissue Engineering

ROSA ALICIA SAUCEDO ACUÑA MONICA GALICIA GARCIA JUDITH VIRGINIA RIOS ARANA SIMON YOBANNY REYES LOPEZ (2012, [Artículo])

Hoy en día, los defectos óseos representan uno de los casos de mayor impacto en la salud debido a la frecuencia con que éstos ocurren a causa de traumatismos, fracturas, enfermedades congénitas o degenerativas. En la actualidad, los implantes de tejido óseo de gran volumen se encuentran severamente restringidos a causa de las limitaciones de difusión en la interacción con el ambiente del huésped para los nutrientes, intercambio gaseoso y eliminación de desechos. Es por ello que la corrección de los defectos óseos ha cobrado gran importancia en el área de Ingeniería tisular buscando mejorar las estrategias clínicas para su tratamiento. El propósito de esta revisión es proporcionar un panorama general del desarrollo de andamios para la regeneración de tejido óseo, mostrando los avances logrados en los ensayos in vitro e in vivo en la última década

Currently, bone defects cases represent a major impact on health due to how often they occur because of trauma, fractures, congenital or degenerative diseases. Now, bone implants to large volume are severely restricted because of the diffusion limitations in the interaction

with the environment of the host for nutrients, gas exchange and waste disposal. That is why the correction of bone defects has become very important in the field of tissue engineering looking to improve clinical strategies for treatment. The purpose of this review is to provide an overview of the development of scaffolds for bone tissue regeneration, showing the progress made in the in vitro and in vivo in recent decades.

MEDICINA Y CIENCIAS DE LA SALUD Ingeniería tisular regeneración ósea Andamio Tissue engineering Bone regeneration Scaffolds

MÉTODOS ÓPTICOS PARA LA DETECCIÓN DEL FENÓMENO FOTOACÚSTICO Y SU APLICACIÓN EN LA RECONSTRUCCIÓN DE IMÁGENES

Yanier Ojeda (2023, [Tesis de doctorado])

"El efecto fotoacústico es un fenómeno físico aplicado en la imagenología biomédica. Desarrollar métodos innovadores para la detección de señales ultrasónicas inducidas mediante la absorción de pulsos láser es determinante para ampliar el rango de operación de las pruebas fotoacústicas. En este trabajo, motivados por su sensibilidad y ancho de banda, se exploraron un conjunto de métodos ópticos para la detección de ultrasonido. Se evaluó un método de campo completo basado en un sistema Schlieren que permitió capturar una instantánea del campo de presiones que se propagó desde la muestra hacia el medio circundante."

Fotoacústica Schlieren Ultrasonido Tomografía Imágenes Óptica CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA FÍSICA ÓPTICA OPTICA FÍSICA OPTICA FÍSICA

Operaciones crud en datos semiestructurados.

SEVERINO FELICIANO MORALES Edgardo Solis Carmona José Luis Hernández-Hernández Mario Hernández Hernández Valentin Alvarez Hilario (2019, [Artículo])

La estructura de los datos puede evolucionar frecuentemente, sin necesidad de que haya cambios en la estructura

de un esquema. La principal característica de este tipo de datos, es el hecho de que no depende de una estructura de un esquema explicito pero existe implícitamente entre los datos o el código. Se puede saber qué tipo de datos se necesitan para obtener un análisis completo de una problemática, para ofrecer soluciones, a pesar de ser schemaless. Los tipos de datos se pueden identificar como Estructurados (Bases de Datos Relacionales, Data Warehouses), Semiestructurados (JSON, XML) y No Estructurados (Texto, Audio y Video), pero lo más que han tomado mayor relevancia son los dos primeros, ya que hacen más fácil el tratamiento de los datos. En este artículo se pretenden abordar los datos semiestructurados y las operaciones fundamentales para los datos en una base de datos NoSQL, específicamente MongoDB.

Datos Semiestructurados Bases de Datos NoSQL Schemaless MongoDB INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS PROCESOS TECNOLÓGICOS

Socio-technological responses to COVID-19 in Mexico: Spin-offs, repositories and 3D printing

Max Matus (2023, [Artículo, Artículo])

The objective of the article is to analyze two sociotechnical initiatives, one citizen and the other governmental, that were configured in Mexico as a response to the shortage and hoarding of medical devices in the context of the COVID-19 pandemic. The citizen response analyzed is the coronavirus_maker_mx movement, while the governmental initiative under scrutiny are the Gätsy and Ehecátl 4T ventilators developed under the coordination of the National Council on Science and Technology. The methodology used involved online tracking of coronavirus_maker groups, open interviews with community members, and review of second-hand sources in the case of the government initiative. By unraveling the networks of actors that were behind the design and production or printing of devices to address COVID-19, we conclude that the cases analyzed represent different models of technological development that invite us to think about some alternatives for the promotion of "sovereign" technology in Mexico.

Impresión 3D manufactura aditiva makers ciencia abierta CIENCIAS SOCIALES CIENCIAS SOCIALES 3D printing additive manufacture open science

Economic valuation of climate induced losses to aquaculture for evaluating climate information services in Bangladesh

Peerzadi Rumana Hossain T.S Amjath-Babu Timothy Joseph Krupnik (2023, [Artículo])

Very little research has focused on climate impacts on aquaculture and the potential of climate information services (CIS) for aquaculture to support sustainable development goals 2030 (SDGs)1. This study represents an effort to bridge this gap by conducting a first ex-ante economic evaluation of CIS for aquaculture in Bangladesh by semi-automating the extraction of data on climate-induced fish losses during 2011 to 2021 from popular online newspaper articles and corroborating them with available government and satellite datasets. During this period, Bangladesh faced an estimated loss of around 140 million USD for hatcheries, open water fish and shrimp. When validated with a year of country-wide official data on climate-induced economic losses to aquaculture, the damage reported from these media sources is approximately 10 percent of actual losses. Given this rule of thumb, the potential economic value of aquacultural CIS could be up to USD14 million a year, if 10 percent of the damage can be offset by appropriate services through a range of multi-sector efforts to establish and extend these services to farmers at scale.

Climate Information Services Newspaper Scraping CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA LOSSES AQUACULTURE CLIMATE SERVICES SUSTAINABLE DEVELOPMENT GOALS