Nitrogen fertilizer application alters the root endophyte bacterial microbiome in maize plants, but not in the stem or rhizosphere soil

Alejandra Miranda Carrazco Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Plant-associated microorganisms that affect plant development, their composition, and their functionality are determined by the host, soil conditions, and agricultural practices. How agricultural practices affect the rhizosphere microbiome has been well studied, but less is known about how they might affect plant endophytes. In this study, the metagenomic DNA from the rhizosphere and endophyte communities of root and stem of maize plants was extracted and sequenced with the “diversity arrays technology sequencing,” while the bacterial community and functionality (organized by subsystems from general to specific functions) were investigated in crops cultivated with or without tillage and with or without N fertilizer application. Tillage had a small significant effect on the bacterial community in the rhizosphere, but N fertilizer had a highly significant effect on the roots, but not on the rhizosphere or stem. The relative abundance of many bacterial species was significantly different in the roots and stem of fertilized maize plants, but not in the unfertilized ones. The abundance of N cycle genes was affected by N fertilization application, most accentuated in the roots. How these changes in bacterial composition and N genes composition might affect plant development or crop yields has still to be unraveled.

Bacterial Community Structure DArT-Seq Bacterial Community Functionality Genes Involved in N Cycling CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURAL PRACTICES MAIZE RHIZOSPHERE STEMS NITROGEN FERTILIZERS

Analyzing antifragility among smallholder farmers in Bihar, India: An assessment of farmers' vulnerability and the strengths of positive deviants

Roos Adelhart Toorop Santiago Lopez-Ridaura ML JAT Deepak Bijarniya Jeroen Groot (2023, [Artículo])

Farmers around the world are increasingly vulnerable: climate variability is identified as the primary stressor, but unfavorable biophysical circumstances and disturbances in the socioeconomic domain (labor dynamics and price volatility) also affect farm management and production. To deal with these disturbances, adaptations are recognized as essential. Antifragility acknowledges that adaptations and volatility are inherent characteristics of complex systems and abandons the idea of returning to the pre-disturbance system state. Instead, antifragility recognizes that disturbances can trigger reorganization, enabling selection and removal of weaker system features and allowing the system to evolve toward a better state. In this study, we assessed the vulnerability of different types of smallholder farms in Bihar, India, and explored the scope for more antifragile farming systems that can 'bounce back better' after disturbances. Accumulation of stocks, creation of optionality (i.e., having multiple options for innovation) and strengthening of farmer autonomy were identified as criteria for antifragility. We had focus group discussions with in total 92 farmers and found that most expressed themselves to be vulnerable: they experienced challenges but had limited adaptive capacity to change their situation. They mostly made short-term decisions to cope with or mitigate urgent challenges but did not engage in strategic planning driven by longer-term objectives. Instead, they waited for governmental support to improve their livelihoods. Despite being confronted with similar challenges, four positive deviant farmers showed to be more antifragile: their diverse farming systems were abundant in stocks and optionality, and the farmers were distinguished in terms of their autonomy, competence, and connectedness to peers, the community, and markets. To support antifragility among regular farmers, adaptations at policy level may be required, for example, by shifting from a top-down toward a bottom-up adaptation and innovation regime where initiative and cooperation are encouraged. With a more autonomous orientation, farmers' intrinsic motivation is expected to increase, enabling transitions at the farm level. In this way, connected systems can be developed which are socioeconomically and biophysically adaptive. When practices, knowledge, and skills are continuously developed, an antifragile system with ample stocks and optionality may evolve over time.

Autonomy Adaptive Capacity Smallholder Farmers CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA POLICIES SMALLHOLDERS AGRICULTURAL PRACTICES

Pathways from information to the adoption of conservation agriculture practices in Malawi and Tanzania

Paswel Marenya Dil Bahadur Rahut (2023, [Artículo])

To reduce agriculture's carbon, land and water footprint, the diffusion of conservation farming methods is one commonly cited proposition. Yet the process of translating available information on new conservation farming methods into farmers' practices is often a black box in many studies. This understanding is critical to inform strategies for scaling these complex, knowledge-intensive, but necessary practices for improving agriculture's resource and climate balance sheet. By implementing a series of mediation analysis using data from 700 households in Malawi and 930 households in Tanzania, this study examines how an improved understanding of conservation agriculture (CA) principles is an important mediator in the pathway from extension contact to the adoption of two of the CA practices examined. For the adoption of conservation tillage, the share of the mediated treatment effect was in the 31.5–34.4% range, while it was 31.6–46.9% for the adoption of soil cover (mulching). Our results suggest that unless learning from external sources strongly correlates with improved farmers' technical understanding of new farming practices, private learning by doing must be a critical adjunct to other avenues of learning. Beyond the basic promotional goals, improving farmers' technical know-how needs to be the centerpiece of holistic efforts in support of conservation farming and similar knowledge-intensive practices necessary for agriculture's sustinability goals.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CONSERVATION AGRICULTURE SMALLHOLDERS AGRICULTURAL PRACTICES FIELD EXPERIMENTATION

Tackling Maize Lethal Necrosis (MLN) in eastern Africa through effective phytosanitary approaches

Suresh L.M. Yoseph Beyene Dan Makumbi Manje Gowda Prasanna Boddupalli (2023, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE NECROSIS DISEASE MANAGEMENT PLANT HEALTH GENE EDITING GERMPLASM

Mulch application as the overarching factor explaining increase in soil organic carbon stocks under conservation agriculture in two 8-year-old experiments in Zimbabwe

Regis Chikowo Christian Thierfelder Marc Corbeels (2024, [Artículo])

Conservation agriculture (CA), combining reduced or no tillage, permanent soil cover, and improved rotations, is often promoted as a climate-smart practice. However, our understanding of the impact of CA and its respective three principles on top- and subsoil organic carbon stocks in the low-input cropping systems of sub-Saharan Africa is rather limited. This study was conducted at two long-term experimental sites established in Zimbabwe in 2013. The soil types were abruptic Lixisols at Domboshava Training Centre (DTC) and xanthic Ferralsol at the University of Zimbabwe farm (UZF). The following six treatments, which were replicated four times, were investigated: conventional tillage (CT), conventional tillage with rotation (CTR), no tillage (NT), no tillage with mulch (NTM), no tillage with rotation (NTR), and no tillage with mulch and rotation (NTMR). Maize (Zea mays L.) was the main crop, and treatments with rotation included cowpea (Vigna unguiculata L. Walp.). The soil organic carbon (SOC) concentration and soil bulk density were determined for samples taken from depths of 0–5, 5–10, 10–15, 15–20, 20–30, 30–40, 40–50, 50–75 and 75–100 cm. Cumulative organic inputs to the soil were also estimated for all treatments. SOC stocks at equivalent soil mass were significantly (p<0.05) higher in the NTM, NTR and NTMR treatments compared with the NT and CT treatments in the top 5 cm and top 10 cm layers at UZF, while SOC stocks were only significantly higher in the NTM and NTMR treatments compared with the NT and CT treatments in the top 5 cm at DTC. NT alone had a slightly negative impact on the top SOC stocks. Cumulative SOC stocks were not significantly different between treatments when considering the whole 100 cm soil profile. Our results show the overarching role of crop residue mulching in CA cropping systems with respect to enhancing SOC stocks but also that this effect is limited to the topsoil. The highest cumulative organic carbon inputs to the soil were observed in NTM treatments at the two sites, and this could probably explain the positive effect on SOC stocks. Moreover, our results show that the combination of at least two CA principles including mulch is required to increase SOC stocks in these low-nitrogen-input cropping systems.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SOIL ORGANIC CARBON CONSERVATION AGRICULTURE EXPERIMENTATION CROP MANAGEMENT

Conservation agriculture practices towards regenerative agriculture

ML JAT (2021, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CONSERVATION AGRICULTURE AGRICULTURAL PRACTICES REGENERATION

MLN disease diagnostics, MLN disease-free seed production and MLN disease management

Suresh L.M. (2022, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA DISEASES DISEASE MANAGEMENT SEED PRODUCTION MAIZE NECROSIS YIELD LOSSES ECONOMIC IMPACT SURVEILLANCE SYSTEMS TRAINING

Genotype by Environment Interaction (GEI) of maize varieties resistant to maize lethal necrosis for Mid Altutides of Rwanda

Claver Ngaboyisonga Suresh L.M. (2022, [Artículo])

Additive Main Effect and Multiplicative Interaction Area Under Disease Progress Curve Maize Lethal Necrosis Variety x Environment Interaction CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE CHLOROTIC MOTTLE VIRUS RESISTANCE VARIETIES INOCULATION DISEASE MANAGEMENT SUGARCANE MOSAIC VIRUS

Soil analysis and integrated nutrient management

Isaiah Nyagumbo (2021, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SOIL ANALYSIS NUTRIENT MANAGEMENT SOIL FERTILITY

Unpacking the intra-household decision-making process among wheat growers in Bihar, India

Hom Nath Gartaula (2022, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT CROP MANAGEMENT SMALLHOLDERS GENDER HOUSEHOLDS