Propuesta de mejora para la integración del equipo de salud en el código azul en áreas críticas adulto

DANIEL GARCIA NAVARRETE (2023, [Otro, Trabajo terminal, especialidad])

La presente investigación se realizará en un hospital de segundo nivel de atención de la ciudad de Cuernavaca, el código azul es un sistema de alarma que existe en la mayoría de los hospitales, está conformado por un equipo multidisciplinario de salud que se encuentra capacitado y es convocado de manera inmediata con funciones previamente asignadas para poder intervenir de forma oportuna, eficaz y con calidad a pacientes en paro cardiopulmonar. Este trabajo de investigación propone la integración del equipo de salud en código azul.

Se menciona como fue la historia de la formación de los grupos de reanimación cardiopulmonar y la organización del código azul; fue el primer modelo de estos sistemas y ha demostrado un impacto positivo en la morbimortalidad de los pacientes, la conexión de la teórica Patricia Benner de principiante a experto, se describe como se vive un código azul en el área hospitalaria, la necesidad de estructurar tarjetas que identifique que rol tiene cada uno de los integrantes del código azul, el personal de salud tiene que tener capacidad de reconocer la emergencia del evento, realizando oportunamente las intervenciones del código azul. Metodología adecuada para esta investigación: se decidió un estudio de tipo cuantitativo, longitudinal, observacional, cuasiexperimental y de intervención educativa, será dirigido a todo profesional de salud; área médica, enfermería, camilleros, residentes, pasantes y estudiantes de ambas profesiones de los diferentes turnos que laboran en las áreas críticas del hospital. Se pretende aplicar dos instrumentos de evaluación diagnóstica y de habilidades previo a la capacitación curso- taller con la finalidad de fortalecer los conocimientos y habilidades y posteriormente aplicar los mismos instrumentos de evaluación final. Se implementará un pizarrón en cada servicio, donde describa el rol de la actividad que tendrá cada integrante que conforme el equipo de código azul, así mismo se proporcionará el manual de procedimientos de código azul, guía de práctica clínica impresa que se ubicaran en un lugar estratégico para su consulta y gafetes que describa la función que le corresponde a cada uno de los miembros.

This research will be carried out in a second level care hospital in the city of Cuernavaca. The code blue is an alarm system that exists in most hospitals; it is made up of a multidisciplinary health team that is trained and immediately summoned with previously assigned functions in order to intervene in a timely, effective and quality manner in patients in cardiopulmonary arrest. This research work proposes the integration of the health team in code blue.

It mentions the history of the formation of cardiopulmonary resuscitation groups and the organization of the code blue; it was the first model of these systems and has shown a positive impact on patient morbidity and mortality, the connection of the theorist Patricia Benner from beginner to expert, it describes how a code blue is experienced in the hospital area, the need to structure cards that identify the role of each of the members of the code blue, the health personnel must have the ability to recognize the emergency event, performing timely interventions of the code blue. Appropriate methodology for this research: a quantitative, longitudinal, observational, quasi-experimental, quasi-experimental and educational intervention study was decided, and it will be directed to all health professionals; medical area, nursing, orderlies, residents, interns and students of both professions of the different shifts that work in the critical areas of the hospital. It is intended to apply two diagnostic and skills evaluation instruments prior to the training course-workshop in order to strengthen knowledge and skills and then apply the same instruments for the final evaluation. A blackboard will be implemented in each service, describing the role of the activity that each member of the code blue team will have, as well as the code blue procedure manual, printed clinical practice guide that will be placed in a strategic place for consultation and badges that describe the function that corresponds to each of the members.

MEDICINA Y CIENCIAS DE LA SALUD CIENCIAS MÉDICAS código azul, integración, equipos de respuesta rápida, personal de salud, paro cardiopulmonar. code blue, integration, rapid response teams, health personnel, cardiopulmonary arrest.

Recent advances on research of native prawn Macrobrachium americanum (Decapoda: Palaemonidae) with aquaculture and conservation purposes

Yuneisy Milagro Agüero Fernández MIRIAM VICTORIA MARTIN MANZO MARITZA LOURDES SOBERANES YEPIZ Marcelo Ulises García Guerrero Yuniel Méndez Martínez Luis Hector Hernandez Hernandez Laura S López Greco Edilmar Cortés Jacinto (2022, [Artículo])

"Where good management practices on aquaculture are mandatory. The economic and ecological importance of prawns of the Genus Macrobrachium cause an impact at a global level involving economic, academic and social aspects. Macrobrachium americanum appears as one of the genus species with high nutritional value and an economic demand in the national and international markets, as well as a vital income for fisherman and producers of this species. For researchers, it is a challenge to find solutions to culture and propose conservation measures for M. americanum with emphasis on development, nutrition and reproduction. Although there are scientific studies supporting the economic importance of this species, our knowledge about its cultivation, reproduction and conservation is limited. This paper summarizes the latest studies made in cooperation with M. americanum in research lead by the Centro de Investigaciones Biológicas del Noroeste, Mexico. After several years of continuous research, it is considered that those efforts have produced useful information for the sustainable exploitation, conservation and basic management practices of this species."

Freshwater ecosystem, growth rate, native prawn species, conservation, sustainable fishing BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) ECOLOGÍA ANIMAL ECOLOGÍA ANIMAL

Effect of Nitrogen, Phosphorus and Potassium on regional organic substrates in Agave salmiana production in Huichapan, Hidalgo, Mexico

EMILIO RAYMUNDO MORALES MALDONADO MONICA GUTIERREZ ROJAS RAMON JAIME HOLGUIN PEÑA Daniel Ruiz-Juárez Jorge Luis Vega Chávez Ana Cristina Reyes Godoy (2022, [Artículo])

"Mexico has 159 species of Agave spp. In the agri-food industry stand out are Agave tequilana, A. angustifolia, and A. salmiana. A limitation to producing maguey seedlings is the low availability of organic substrates that favor plant adaptation in the field. The objective was to evaluate the effect of nitrogen (N), phosphorus (P) and potassium (K) found in substrates in response to vegetative and root growth of A. salmiana in agricultural areas of Huichapan, Hidalgo, Mexico. The treatment consisted of earthworm humus (EH) and leaf compost (LC) substrates with materials from the region with different percentages of EH (100, 75, 50%), LC (5, 10%), and sand (20, 40%). The treatments were applied with 14 random replications in two phases in seeds and 40-day-seedlings. The variables evaluated were NPK amount and pH in substrates. The physiological variables measured were plant height, leaf number, stem diameter, root length, and volume. Significant differences (P≤0.05) were observed in seedling physiology due to the effect of the treatment. The best agronomic responses (plant growth and root length/weight) of Agave seedlings were T5 (75% earthworm humus + 20% sand + 5% leaf-soil) and T6 (50% earthworm humus + 40% sand + 10% leaf-soil); in both treatments, the NPK percentages were different from the control (Haplic Phaeozem soil) group. The final concentration of NPK in T6 was N = 0.04%, P = 398.13 mg Kg-1 and K = 11.88 meq 100g-1 . The results infer that NPK availability in soil and progressive acidification (initial pH = 8.6, final pH = 7.4) of the substrate can favorably influence the plant response. The interactions between NPK availability in the substrate and their use for a better response in maguey seedling adaptability open up new lines of research on the productive systems in the región of Huichapan, Hidalgo, Mexico."

maguey, nutritional quality, plant physiology, productive soils, seedling BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) FERTILIDAD DEL SUELO FERTILIDAD DEL SUELO

Indicadores de estrés y desempeño biológico de juveniles de lobina rayada (Morone saxatilis) en agua marina: efecto de la densidad de cultivo y color del tanque

Stress indicators and biological performance of striped bass (Morone saxatilis) juvenile in seawater: effect stock density and tank color

DENISE ENCARNACION MAYORAL NORIEGA (2023, [Tesis de maestría])

Existen factores ambientales de naturaleza física, química o biológica que afectan directamente la alimentación de los peces. Por ejemplo, las altas densidades de cultivo pueden generar estrés en los organismos, resultando en la merma del consumo de alimento y por ende su crecimiento. Sin embargo, algunas especies toleran altas densidades formando un grupo o cardumen que les confiere un mejor desempeño. Otro factor que afecta el comportamiento de algunas especies es la coloración de los tanques de cultivo; demostrando la necesidad de utilizar tanques con un entorno físico adecuado para la especie a cultivar. Actualmente, M. saxatilis se produce en tanques en laboratorio y se engorda en jaulas marinas en Baja California; así que existe un gran interés por mejorar su crecimiento y eficiencia alimenticia. Por ello, el objetivo de esta investigación fue evaluar tres densidades de cultivo (alta: 6.2 kg·m-3, media: 3.1 kg·m-3 y baja: 1.5 kg·m-3) y dos colores de tanque (azul celeste y negro) en el crecimiento, supervivencia, utilización del alimento, índices corporales y concentración de cortisol y glucosa de juveniles de lobina rayada en agua marina para su mantenimiento en laboratorio durante nueve semanas. Los resultados demostraron que los organismos obtuvieron el mayor crecimiento en peso en la densidad media, indistintamente del color del tanque. Asimismo, la densidad afectó significativamente los índices hepatosomático y liposomático, ya que disminuyeron conforme aumentó la densidad. El color del tanque influyó de manera importante en el consumo de alimento (azules: 37.4 ± 1.9 g·pez-1; negros: 35.9 ± 2.0 g·pez-1). La interacción de los factores afectó la TCE, TCA y TEP. Las concentraciones de cortisol (81.8 ± 22.5 ng·ml-1) y glucosa (83.2 ± 11.4 mg·100 ml-1) fueron similares en todos los tratamientos y relativamente bajas para la especie. En conclusión, se logró elucidar bajo qué condiciones de cultivo en laboratorio los organismos lograron superar los factores de estrés sin comprometer su crecimiento y supervivencia, reflejándose en un mejor desempeño biológico. Se recomienda utilizar tanques azules o negros con densidades iniciales de 4.2 kg·m-3 o 233 peces·m-3 para el cultivo de juveniles de M. saxatilis en agua salada bajo condiciones de laboratorio.

Environmental factors of a physical, chemical or biological nature such as high stocking densities can directly affect fish feeding and generate stress in organisms, resulting in a decrease in food intake and therefore growth. However, some species tolerate high densities, forming a group or shoal resulting in better overall performance. Another factor that influences the behavior of some species is the color of the culture tank; warranting the need to use tanks with a physical environment suitable for the species to be cultivated. Currently, striped bass are produced in tank-based hatcheries to be socked in sea cages for growout in Baja California. Thus, there is interest in improving their growth rate and feed efficiency under laboratory conditions. Therefore, the objective of this research was to evaluate the effect of three stocking densities (high: 6.2 kg m-3, medium: 3.1 kg m-3 and low: 1.5 kg m-3) and two tank colors (light blue and black) on the biological performance (growth, survival, food utilization), somatic indices and fish stress using the concentration of cortisol and glucose in blood serum as response variables of juvenile striped bass cultured in seawater for nine weeks. The results showed that the organisms had greater growth in terms of final weight when they were cultured at the medium stocking density, regardless of tank color. Likewise, density had a significant effect on hepatosomatic and liposomatic indices, with a tendency to decrease as density increased. The color of the tank significantly influenced the food consumption (blues: 37.4 ± 1.9 g·fish-1; blacks: 35.9 ± 2.0 g·fish-1). The interaction of the factors influenced TCE, TCA and TEP. The concentrations of cortisol (81.8 ± 22.5 ng·ml-1) and glucose (83.2 ± 11.4 mg·100 ml-1) were similar in all treatments indicating low fish stress. In conclusion, it was possible to elucidate under which laboratory culture conditions the organisms were able to overcome the stress factors without compromising their growth and survival; reflected in better biological performance. A stocking density of 4.2 kg m-3 o 233 fish·m-3 for M. saxatilis juvenile reared in saltwater under laboratory culture conditions is recommended.

lobina rayada, densidad cultivo, color tanque, estrés, crecimiento striped bass, stocking density, tank color, stress, growth CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE PISCICULTURA PISCICULTURA

Gene expression in primary hemocyte culture of the Pacific white shrimp Penaeus vannamei infected with different white spot syndrome virus (WSSV) strains

Delia Patricia Parrilla Taylor REGINA ELIZONDO GONZALEZ Jesús Neftalí Gutiérrez Rivera SILVIA ALEJANDRA GARCIA GASCA NORBERTO VIBANCO PEREZ MA. DE JESUS DURAN AVELAR RICARDO VAZQUEZ JUAREZ (2022, [Artículo])

"Five previously analyzed white spot syndrome virus (WSSV) strains from northwest Mexico,differing in their genome architecture as well as in virulence, were selected (high virulence JP and LG strains;moderate virulence GVE and DIV strains; and low virulence LC10 strain) to evaluate pathogenesis response in vitro. Expression of phagocytosis-activating protein PAP, manganese superoxide dismutase MnSOD and peroxiredoxin PRX, and two genes of immediate-early expression (IE1 and WSSV304) were measured by qPCR in a primary hemocyte cell culture from Penaeus vannamei at 1, 3, 6, 12, and 24 h post-infection (hpi). PAP expression was significantly higher at 1 and 3 hpi, and JP and LC10 strains induced the highest expression. The response of MnSOD was high at 1 hpi, and a significant increase in PRX expression was detected at 3 hpi, probably due to the occurrence of an oxidative burst; expression levels of MnSOD and PRX were significantly higher at 1 and 3 hpi, respectively, induced by the LG strain (high virulence), suggesting an acute response. In general, expression of most immune-related - genes decreased after the initial hours of infection. Expression levels of IE1 and WSSV304 were exceptionally high at 1 hpi in almost all five WSSV analyzed strains,confirming their efficient mechanism for replication and viral fitness. The results of this study do not show an accurate link between the genome size and WSSV virulence of the strains, albeit the strain with the smallest genome showed the highest virulence. All strains induced an early immune response in heterogeneous ways."

Penaeus vannamei, virulence, gene expression, viral fitness, viral pathogenesis, immune response BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) PATOLOGÍA ANIMAL PATOLOGÍA ANIMAL

PROPUESTA DE MANEJO DE LA PESQUERÍA EMERGENTE DE MERLUZA DEL PACÍFICO NORTE Merluccius productus EN EL GOLFO DE ULLOA, BAJA CALIFORNIA SUR

PABLO MORA ZAMACONA (2023, [Tesis de doctorado])

"La merluza del Pacífico Merluccius productus se distribuye desde Alaska hasta el sur de México, con una población particular descrita al sur de la península de Baja California conocida como merluza enana, de la que poco se sabe. Dicha población actualmente no es pescada comercialmente, por lo que representa un recurso con potencial de explotación. Las pesquerías potenciales y emergentes crean desafíos y oportunidades para los administradores pesqueros que necesitan tomar decisiones sobre cómo gestionar de forma sostenible una pesquería previa a su inicio. Este trabajo propone y analiza posibles medidas alternativas de gestión pesquera para esta potencial pesquería, bajo incertidumbre biológica y de mercado. Debido al desconocimiento de diversos aspectos de la biología del recurso primeramente se analizó el crecimiento individual del stock y posteriormente se realizó un análisis bioeconómico de la potencial pesquería.

Para el análisis del crecimiento individual se utilizó un total de 240 otolitos y datos de longitud patrón de 932 especímenes muestreados de mayo a diciembre de 2015. La formación anual de incrementos en los otolitos se verificó mediante la relación de incremento marginal y el análisis de borde. La edad se estimó a partir de recuentos de anillos en secciones sagitales de otolitos y el crecimiento se analizó mediante un enfoque de inferencia multimodelo. Se consideraron los modelos de crecimiento de von Bertalanffy (VBGM), VBGM generalizado, Gompertz, Logístico y Johnson, los cuales se ajustaron a conjuntos de datos de talla a la edad observados y retrocalculados agrupados por sexo. Según el criterio de información de Akaike, el VBGM generalizado fue el más apropiado para las hembras (L∞= 31.36 cm, k= 0.15 cm año-1), mientras que el VBGM proporcionó el mejor ajuste para los machos (L∞= 25.35 cm, k= 0.28 cm año-1).

El análisis bioeconómico de alternativas de manejo para una potencial pesquería de merluza enana indicó que una nueva pesquería de dicho stock podría ser biológicamente sostenible y económicamente rentable, bajo un conjunto de estrategias de gestión y reglas de control. Se recomienda un acceso limitado con bajo esfuerzo pesquero ya que resulta más redituable por barco y biológicamente precautorio, considerando la alta incertidumbre asociada con la explotación de una población virgen..."

"The Pacific hake Merluccius productus is distributed from Alaska to southern Mexico, with a particular population described south of the Baja California peninsula known as dwarf hake, about which little is known. This population is not currently commercially fished; hence it represents a resource with potential for exploitation. Potential and emerging fisheries create challenges and opportunities for fishery managers who need to make decisions about how to sustainably manage a fishery prior to its initiation. This work proposes and analyzes possible alternative fishery management measures for this potential fishery, under biological and market uncertainty. Due to the lack of knowledge of various aspects of the biology of the resource, the individual growth of the stock was first analyzed and subsequently a bioeconomic analysis of the potential fishery was carried out.

A total of 240 otoliths and standard-length data from 932 specimens sampled from May to December 2015 were used for the analysis of individual growth. The annual formation of otolith increments was verified using the marginal increment analysis and the edge analysis. Age was estimated from annuli counts in sagittal sections of otoliths and growth was analyzed using a multi-model inference approach. The von Bertalanffy growth models (VBGM), generalized VBGM, Gompertz, Logistic and Johnson growth models were considered, which were fitted to observed and back calculated length-at-age data sets grouped by sex. According to Akaike's information criterion, the generalized VBGM was the most appropriate for females (L∞= 31.36 cm, k= 0.15 cm year-1), while the VBGM provided the best fit for males (L∞= 25.35 cm, k= 0.28 cm year-1).

The bioeconomic analysis of management alternatives for a potential dwarf hake fishery indicated that a new fishery for said stock could be biologically sustainable and economically profitable, under a set of management strategies and control rules. Limited access with low fishing effort is recommended as it is more profitable by boat and biologically precautionary, considering the high uncertainty associated with the exploitation of an unfished stock. Despite the combination of high fishing costs and low prices, the fishery could still be profitable in the long term, although there is risk of overexploitation if high fishing effort is allowed..."

Merluza enana, stock no explotado, crecimiento individual, análisis bioeconómico, pesquería potencial Dwarf hake, unexploited stock, individual growth, bioeconomic analysis, potential fishery CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE REGLAMENTACIÓN Y CONTROL REGLAMENTACIÓN Y CONTROL

Growth Promoting Activity of Annona muricata L. Leaf Extracts on Lactobacillus casei

NIMCY NOEMI MEZA GUTIERREZ Paola Magallón Servín ROSENDO BALOIS MORALES IZA FERNANDA PEREZ RAMIREZ GRACIELA GUADALUPE LOPEZ GUZMAN GUILLERMO BERUMEN VARELA Pedro Ulises Bautista Rosales (2022, [Artículo])

"Soursop leaves are a source of phytochemical compounds, such as phenolic acids, flavonoids, hydrolyzable tannins, and acetogenins. These compounds can have several types of biological activities. Lactic acid bacteria can uptake phenolic compounds present in plants or fruits. The aim of the present work was to investigate the in vitro effect of hexane, acetone, methanolic, and aqueous extracts of soursop leaves (Annona muricata L.) on the growth, motility, and biofilm formation of Lactobacillus casei, and to determine compounds related to growth. The minimum concentration promoting growth, motility (swimming, swarming, and twitching), and biofilm-forming capacity (crystal violet) were evaluated. The results showed the growth-promoting capacity of acetone and aqueous extracts at low doses 25–50 mg/L, and an inhibition in the four extracts at higher doses of 100 mg/L. The L. casei growth is related to ellagic acid, quercetin rhamnoside, kaempferol dihexoside, quercetin hexoside, secoisolariciresinol, and kaempferol hexoside-rhamnoside. Hexane extract increased the three types of motility, while aqueous maintained swimming and twitching motility similar to control. The four extracts inhibited the biofilm formation capacity."

prebiotic, Lactobacillus, polyphenol, probiotic, growth promoters, soursop leaves CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS AGROQUÍMICA REGULADORES DEL CRECIMIENTO DE LAS PLANTAS REGULADORES DEL CRECIMIENTO DE LAS PLANTAS

Isolation and characterization of endophytic bacteria associated with roots of jojoba (Simmondsia chinensis (Link) Schneid)

RICARDO VAZQUEZ JUAREZ TANIA ZENTENO SAVIN ENRIQUE MORALES BOJORQUEZ Elvia Pérez Rosales Lilia Alcaráz Meléndez María Esther Puente Eduardo Quiroz Guzmán (2017, [Artículo])

"In this communication, the diversity and beneficial characteristics of endophytic bacteria have been studied in Simmondsia chinensis that has industrial importance because of the quality of its seed oil. Endophytes were isolated (N = 101) from roots of the jojoba plants collected, of which eight were identified by partial sequencing of the 16S rDNA gene. The isolated bacteria were Bacillus sp., Methylobacterium aminovorans, Oceanobacillus kimchi, Rhodococcus pyridinivorans and Streptomyces sp. All isolates had at least one positive feature, characterizing them as potential plant growth promoting bacteria. In this study, R. pyridinivorans and O. kimchi are reported as plant growth promoters."

Endophytic bacteria, plant growth promoters, Simmondsia chinensis, seed oil BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA BACTERIOLOGÍA BACTERIOLOGÍA

Movements of scalloped hammerhead sharks (Sphyrna lewini) at Cocos Island, Costa Rica and between oceanic islands in the Eastern Tropical Pacific

Elena Nalesso (2019, [Artículo])

Many species of sharks form aggregations around oceanic islands, yet their levels of residency and their site specificity around these islands may vary. In some cases, the waters around oceanic islands have been designated as marine protected areas, yet the conservation value for threatened shark species will depend greatly on how much time they spend within these protected waters. Eighty-four scalloped hammerhead sharks (Sphyrna lewini Griffith & Smith), were tagged with acoustic transmitters at Cocos Island between 2005–2013. The average residence index, expressed as a proportion of days present in our receiver array at the island over the entire monitoring period, was 0.52±0.31, implying that overall the sharks are strongly associated with the island. Residency was significantly greater at Alcyone, a shallow seamount located 3.6 km offshore from the main island, than at the other sites. Timing of presence at the receiver locations was mostly during daytime hours. Although only a single individual from Cocos was detected on a region-wide array, nine hammerheads tagged at Galapagos and Malpelo travelled to Cocos. The hammerheads tagged at Cocos were more resident than those visiting from elsewhere, suggesting that the Galapagos and Malpelo populations may use Cocos as a navigational waypoint or stopover during seasonal migrations to the coastal Central and South America. Our study demonstrates the importance of oceanic islands for this species, and shows that they may form a network of hotspots in the Eastern Tropical Pacific. © 2019 Nalesso et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

article, Cocos Island, human, monitoring, nonhuman, resident, shark, South America, animal, Costa Rica, environmental protection, island (geological), movement (physiology), physiology, season, shark, Animals, Conservation of Natural Resources, Costa CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Phylogenetic relationships of Pseudo-nitzschia subpacifica (Bacillariophyceae) from the Mexican Pacific, and its production of domoic acid in culture

Sonia Quijano (2020, [Artículo])

Pseudo-nitzschia is a cosmopolitan genus, some species of which can produce domoic acid (DA), a neurotoxin responsible for the Amnesic Shellfish Poisoning (ASP). In this study, we identified P. subpacifica for the first time in Todos Santos Bay and Manzanillo Bay, in the Mexican Pacific using SEM and molecular methods. Isolates from Todos Santos Bay were cultivated under conditions of phosphate sufficiency and deficiency at 16°C and 22°C to evaluate the production of DA. This toxin was detected in the particulate (DAp) and dissolved (DAd) fractions of the cultures during the exponential and stationary phases of growth of the cultures. The highest DA concentration was detected during the exponential phase grown in cells maintained in P-deficient medium at 16°C (1.14 ± 0.08 ng mL-1 DAd and 4.71 ± 1.11 × 10−5 ng cell-1 of DAp). In P-sufficient cultures DA was higher in cells maintained at 16°C (0.25 ± 0.05 ng mL-1 DAd and 9.41 ± 1.23 × 10−7 ng cell-1 of DAp) than in cells cultured at 22°C. Therefore, we confirm that P. subpacifica can produce DA, especially under P-limited conditions that could be associated with extraordinary oceanographic events such as the 2013–2016 "Blob" in the northeastern Pacific Ocean. This event altered local oceanographic conditions and possibly generated the presence of potential harmful species in areas with economic importance on the Mexican Pacific coast. © 2020 Quijano-Scheggia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

domoic acid, domoic acid, kainic acid, Article, cell growth, controlled study, diatom, Mexico, morphology, nonhuman, Pacific Ocean, phylogeny, plant cell, plant growth, Pseudo nitzschia, toxin analysis, cell culture technique, classification, diatom, CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA