Isolation and characterization of endophytic bacteria associated with roots of jojoba (Simmondsia chinensis (Link) Schneid)

RICARDO VAZQUEZ JUAREZ TANIA ZENTENO SAVIN ENRIQUE MORALES BOJORQUEZ Elvia Pérez Rosales Lilia Alcaráz Meléndez María Esther Puente Eduardo Quiroz Guzmán (2017, [Artículo])

"In this communication, the diversity and beneficial characteristics of endophytic bacteria have been studied in Simmondsia chinensis that has industrial importance because of the quality of its seed oil. Endophytes were isolated (N = 101) from roots of the jojoba plants collected, of which eight were identified by partial sequencing of the 16S rDNA gene. The isolated bacteria were Bacillus sp., Methylobacterium aminovorans, Oceanobacillus kimchi, Rhodococcus pyridinivorans and Streptomyces sp. All isolates had at least one positive feature, characterizing them as potential plant growth promoting bacteria. In this study, R. pyridinivorans and O. kimchi are reported as plant growth promoters."

Endophytic bacteria, plant growth promoters, Simmondsia chinensis, seed oil BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA BACTERIOLOGÍA BACTERIOLOGÍA

Fitorremediación asistida a través de bacterias, para suelos contaminados con metales pesados y plaguicidas

TANIA GÓMEZ MORALES (2023, [Otro, Trabajo terminal, especialidad])

El suelo es un recurso no renovable que nos proporciona diferentes beneficios, incluidos los servicios ecosistémicos. Sin embargo, actividades antropogénicas como la minería y la agricultura lo han contaminado. Las prácticas mineras y agrícolas liberan contaminantes como plaguicidas y metales pesados al suelo como contaminantes individuales o mezclas, generando importantes efectos adversos para el ambiente y la salud humana. Debido a esta problemática, se han desarrollado diferentes estrategias de remediación para sitios con presencia de estos co-contaminantes. Una de ellas es la fitorremediación asistida por bacterias, una técnica basada en la asociación de plantas y bacterias, donde ambos organismos se benefician y mejoran la eficiencia de la remediación.

Soil is a non-renewable resource that provides us with different benefits, including ecosystem services. However, anthropogenic activities such as mining and agriculture have contaminated it. Minery and agriculture practices release contaminants such as pesticides and heavy metals into the soil as individual pollutants or mixtures, generating important adverse environmental and human health effects. Due to this problem, different remediation strategies have been developed for sites with the presence of these co-contaminants; one of them is bacterial-assisted phytoremediation, a technique based on the association of plants and bacteria, where both organisms benefit and improve remediation efficiency.

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA suelo, fitorremediación, bacterias, plaguicidas, metales pesados, co-contaminados. soil, phytoremediation, bacteria, pesticides, heavy metals, co-contaminated

Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

CARLOS ABRAHAM GUERRERO RUIZ (2019, [Artículo])

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Análisis isotópico en plumas del Charrán mínimo (Sternula antillarum) para inferir su ecología trófica

Isotope analysis in feathers of the Least Tern (Sternula antillarum) to infer its trophic ecology

Andehui Danay Morales Flores (2024, [Tesis de maestría])

El análisis isotópico de plumas primarias utilizando las razones isotópicas δ13C y δ15N permitió estimar la amplitud y superposición del nicho trófico de los conjuntos de las colonias reproductivas de adultos y volantones del charrán mínimo. Se infirió la amplitud del nicho por medio del área de la elipse estándar estimada por métodos Bayesianos (SEAB), se encontró que existen diferencias entre el nicho trófico de adultos y volantones, pues los adultos tienen valores de la media SEAB de 16.3‰2 hasta 28.4‰2 y los volantones de 44.8‰2 hasta 75.5‰2, esto podría ser debido a diferencias en cuanto a las presas seleccionadas y la ubicación geográfica de los adultos durante la muda de las primarias. Por otro lado, la superposición del nicho indicó la similitud entre los adultos de diferentes colonias, por lo cual, se consideró que los adultos de algunas colonias podrían compartir un sitio de invernada o bien la temporalidad en la muda de las primarias. Además, se cumplió con el objetivo de caracterizar la variabilidad de las firmas isotópicas de carbono y nitrógeno durante el crecimiento secuencial de las plumas primarias por medio de los modelos aditivos generalizados y se observó la variabilidad entre las primarias utilizando las anomalías respecto a la media local de cada colonia, lo cual permitió diferenciar estrategias de alimentación específicas para ciertos conjuntos y la variabilidad en la dieta. La búsqueda bibliográfica de los sitios potenciales de migración en invierno más los mapas de gradientes isotópicos de δ13C permitió determinar que el Océano Pacífico Oriental Tropical es la región geográfica relacionada con la distribución δ13C en plumas primarias del charrán mínimo y es el sitio más probable de invernada.

The isotopic analysis of primary feathers using the isotopic ratios of δ13C and δ15N allowed the estimation of the breadth and overlap of the trophic niche of adult and fledgling least terns. Niche breadth was inferred through the standard ellipse area (SEAB) estimated by Bayesian methods. We found differences between the trophic niche of adults and fledglings, as adults presented mean SEAB values from 16.3‰2 to 28.4‰2 and fledglings from 44.8‰2 to 75.5‰2. This could be due to differences in the prey selected and the geographical location of the adults during the molt of the primaries. The overlap of these values among adults indicates similarity in prey selection and location between the adults of different colonies. Therefore, it was considered that the adults of some colonies could share a wintering site during the period of molt of the primary feathers. We characterized the variability of carbon and nitrogen isotopic signatures during the sequential growth of primary feathers through generalized additive models and the variability between primaries using the anomalies of these signatures and the local mean for each colony. This allowed us to differentiate specific feeding strategies of individual least terns and the variability in their diet. The bibliographic search for potential migration and wintering sites found published maps of δ13C isotopic gradients in the Tropical Eastern Pacific Ocean that correspond to the δ13C distribution in primary feathers of the least tern, indicating a potential wintering area for this species.

isótopos, carbono, nitrógeno, ave marina, ecología trófica isotopes, seabird, nitrogen, carbon, trophic ecology BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) ECOLOGÍA VEGETAL ECOLOGÍA VEGETAL

Vibrissa growth rate in California sea lions based on environmental and isotopic oscillations

MARTHA PATRICIA ROSAS HERNANDEZ (2018, [Artículo])

Pinniped vibrissae provide information on changes in diet at seasonal and annual scales; however, species-specific growth patterns must first be determined in order to interpret these data. In this study, a simple linear model was used to estimate the growth rate of vibrissae from adult female California sea lions (Zalophus californianus) from San Esteban Island in the Gulf of California, Mexico. The δ15N and δ13C values do not display a marked oscillatory pattern that would permit direct determination of the time period contained in each vibrissa; thus, time (age) was calculated in two ways: 1) based on the correlation between the observed number of peaks (Fourier series) in the δ15N profile and the length of each vibrissa, and 2) through direct comparison with the observed number of peaks in the δ15N profile. Cross-correlation confirmed that the two peaks in the δ15N profile reflected the two peaks in the chlorophyll-a concentration recorded annually around the island. The mean growth rate obtained from the correlation was 0.08 ± 0.01 mm d-1, while that calculated based on the observed number of peaks was 0.10 ± 0.05 mm d-1. Both are consistent with the rates reported for adult females of other otariid species (0.07 to 0.11 mm d-1). Vibrissa growth rates vary by individual, age, sex, and species; moreover, small differences in the growth rate can result in significant differences over the time periods represented by the isotopic signal. Thus, it is important to assess this parameter on a species-by-species basis. © 2018 Rosas-Hernández et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

carbon, nitrogen, animal, California, chemistry, diet, female, island (geological), Mexico, Otariidae, physiology, Animals, California, Carbon Isotopes, Diet, Female, Islands, Mexico, Nitrogen Isotopes, Sea Lions BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA INMUNOLOGÍA INMUNOLOGÍA

Spatial Effects of Urban Transport on Air Pollution in Metropolitan Municipalities of Mexico

GERZAIN AVILES POLANCO Marco Antonio Almendárez Hernández Luis Felipe Beltrán Morales Alfredo Ortega Rubio (2022, [Artículo])

"The objective of this work was to estimate the local effects and spatial spillover effects of the number of vehicles, use of urban public transport, and population density on nitrogen oxide emissions for 405 metropolitan municipalities in Mexico in 2016. To this end, a Spatial Durbin Model was estimated. We found positive direct effects of the number of vehicles and population density and negative direct effects of the use of urban public transport. The number of vehicles in circulation had negative spillover effects on the nitrogen oxide emissions of neighboring municipalities. These results indicate that the design of public policy programs aimed at reducing air pollution in Mexico should be based on coordination across metropolitan municipalities."

private car transportation, air pollution, nitrogen oxide emissions, spatial spillover effects, metropolitan municipalities CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO METEOROLOGÍA CONTAMINACIÓN ATMOSFÉRICA CONTAMINACIÓN ATMOSFÉRICA

Trophic ecology of Mexican Pacific harbor seal colonies using carbon and nitrogen stable isotopes

MARICELA JUAREZ RODRIGUEZ (2020, [Artículo])

There is limited information that provides a comprehensive understanding of the trophic ecology of Mexican Pacific harbor seal (Phoca vitulina richardii) colonies. While scat analysis has been used to determine the diet of some colonies, the integrative characterization of its feeding habits on broader temporal and spatial scales remains limited. We examined potential feeding grounds, trophic niche width, and overlap, and inferred the degree of dietary specialization using stable carbon and nitrogen isotope ratios (δ13C and δ15N) in this subspecies. We analyzed δ13C and δ15N on fur samples from pups collected at five sites along the western coast of the Baja California Peninsula, Mexico. Fur of natal coat of Pacific harbor seal pups begins to grow during the seventh month in utero until the last stage of gestation. Therefore pup fur is a good proxy for the mother's feeding habits in winter (∼December to March), based on the timing of gestation for the subspecies in this region. Our results indicated that the δ13C and δ15N values differed significantly among sampling sites, with the highest mean δ15N value occurring at the southernmost site, reflecting a well-characterized north to south latitudinal 15N-enrichment in the food web. The tendency identified in δ13C values, in which the northern colonies showed the most enriched values, suggests nearshore and benthic-demersal feeding habits. A low variance in δ13C and δ15N values for each colony (<1‰) and relatively small standard ellipse areas suggest a specialized foraging behavior in adult female Pacific harbor seals in Mexican waters. © 2020 Juárez-Rodríguez et al.

carbon, delta carbon 13, delta nitrogen 15, isotope, nitrogen, unclassified drug, carbon, nitrogen, Article, correlational study, feeding behavior, latitude, Mexico, nonhuman, organism colony, Pinnipedia, population abundance, species richness, troph BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) BIOLOGÍA ANIMAL (ZOOLOGÍA)

Vibrissa growth rate in California sea lions based on environmental and isotopic oscillations

MARTHA PATRICIA ROSAS HERNANDEZ (2018, [Artículo])

Pinniped vibrissae provide information on changes in diet at seasonal and annual scales; however, species-specific growth patterns must first be determined in order to interpret these data. In this study, a simple linear model was used to estimate the growth rate of vibrissae from adult female California sea lions (Zalophus californianus) from San Esteban Island in the Gulf of California, Mexico. The δ15N and δ13C values do not display a marked oscillatory pattern that would permit direct determination of the time period contained in each vibrissa; thus, time (age) was calculated in two ways: 1) based on the correlation between the observed number of peaks (Fourier series) in the δ15N profile and the length of each vibrissa, and 2) through direct comparison with the observed number of peaks in the δ15N profile. Cross-correlation confirmed that the two peaks in the δ15N profile reflected the two peaks in the chlorophyll-a concentration recorded annually around the island. The mean growth rate obtained from the correlation was 0.08 ± 0.01 mm d-1, while that calculated based on the observed number of peaks was 0.10 ± 0.05 mm d-1. Both are consistent with the rates reported for adult females of other otariid species (0.07 to 0.11 mm d-1). Vibrissa growth rates vary by individual, age, sex, and species; moreover, small differences in the growth rate can result in significant differences over the time periods represented by the isotopic signal. Thus, it is important to assess this parameter on a species-by-species basis. © 2018 Rosas-Hernández et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

carbon, nitrogen, animal, California, chemistry, diet, female, island (geological), Mexico, Otariidae, physiology, Animals, California, Carbon Isotopes, Diet, Female, Islands, Mexico, Nitrogen Isotopes, Sea Lions CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA

Genetic analysis of Vibrio parahaemolyticus O3:K6 strains that have been isolated in Mexico since 1998

CARLOS ABRAHAM GUERRERO RUIZ (2017, [Artículo])

Vibrio parahaemolyticus is an important human pathogen that has been isolated worldwide from clinical cases, most of which have been associated with seafood consumption. Environmental and clinical toxigenic strains of V. parahaemolyticus that were isolated in Mexico from 1998 to 2012, including those from the only outbreak that has been reported in this country, were characterized genetically to assess the presence of the O3:K6 pandemic clone, and their genetic relationship to strains that are related to the pandemic clonal complex (CC3). Pathogenic tdh+ and tdh+/trh+ strains were analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Also, the entire genome of a Mexican O3:K6 strain was sequenced. Most of the strains were tdh/ORF8-positive and corresponded to the O3:K6 serotype. By PFGE and MLST, there was very close genetic relationship between ORF8/O3:K6 strains, and very high genetic diversities from non-pandemic strains. The genetic relationship is very close among O3:K6 strains that were isolated in Mexico and sequences that were available for strains in the CC3, based on the PubMLST database. The whole-genome sequence of CICESE-170 strain had high similarity with that of the reference RIMD 2210633 strain, and harbored 7 pathogenicity islands, including the 4 that denote O3:K6 pandemic strains. These results indicate that pandemic strains that have been isolated in Mexico show very close genetic relationship among them and with those isolated worldwide. © 2017 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial strain, biofouling, controlled study, Crassostrea, food intake, gene sequence, genetic analysis, genetic variability, Japan, Mexican, Mexico, molecular phylogeny, nonhuman, pandemic, pathogenicity island, sea food, serotyping, toxi BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

High quality diet improves lipid metabolic profile and breeding performance in the blue-footed booby, a long-lived seabird

ERICK GONZALEZ MEDINA (2018, [Artículo])

Understanding the role of diet in the physiological condition of adults during reproduction and hence its effect on reproductive performance is fundamental to understand reproductive strategies in long-lived animals. In birds, little is known about the influence of the quality of food consumed at the beginning of the reproductive period and its short-term effects on reproductive performance. To assess the role of diet in the physiological condition of female blue-footed booby, Sula nebouxii (BFBO), during reproduction we evaluated whether individual differences in diet (assessed by using δ13C and δ15N values of whole blood from female birds and muscle tissue of the principal prey species) prior to egg laying and during incubation influenced their lipid metabolic profile (measured as triglyceride levels and C:N ratio) and their reproductive performance (defined by laying date, clutch size and hatching success). Females with higher δ15N values in their blood during the courtship and incubation periods had a higher lipid metabolic profile, earlier laying date, greater clutch size (2–3 eggs) and higher hatching success. Females that laid earlier and more eggs (2–3 eggs) consumed more Pacific anchoveta (Cetengraulis mysticetus) and Pacific thread herring (Opisthonema libertate) than did other females. These two prey species also had high amounts of lipids (C:N ratio) and caloric content (Kcal/g fresh weight). The quality of food consumed by females at the beginning of reproduction affected their physiological condition, as well as their short-term reproductive performance. Our work emphasizes the importance of determining the influence of food quality during reproduction to understand the reproductive decisions and consequences in long-lived animals. © 2018 González-Medina et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

nitrogen 15, triacylglycerol, lipid, animal experiment, Article, breeding, carbon nitrogen ratio, clutch size, controlled study, courtship, diet, egg laying, female, food intake, hatching, lipid metabolism, muscle tissue, nonhuman, prey, reproduction CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA