Repositorio Nacional

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Multimodal deep learning methods enhance genomic prediction of wheat breeding

Carolina Rivera-Amado Francisco Pinto Francisco Javier Pinera-Chavez David González-Diéguez Matthew Paul Reynolds Paulino Pérez-Rodríguez Huihui Li Osval Antonio Montesinos-Lopez Jose Crossa (2023, [Artículo])

Conventional Methods Genomic Prediction Accuracy Deep Learning Novel Methods CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT BREEDING MACHINE LEARNING METHODS MARKER-ASSISTED SELECTION

Satellite imagery for high-throughput phenotyping in breeding plots

Francisco Pinto Mainassara Zaman-Allah Matthew Paul Reynolds Urs Schulthess (2023, [Artículo])

Optimized Soil Adjusted Vegetation Index CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HIGH-THROUGHPUT PHENOTYPING SATELLITES WHEAT MAIZE BREEDING NORMALIZED DIFFERENCE VEGETATION INDEX

Establishment of heterotic groups for hybrid wheat breeding

Yunbi Xu (2022, [Artículo])

Genomic Prediction CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CLIMATE CHANGE CROPS FORECASTING PLANTS COMBINING ABILITY HETEROSIS HETEROTIC GROUPS MALE INFERTILITY PLANT HEIGHT WHEAT

CIMMYT seed systems interventions

lessons to Bangladesh

AbduRahman Issa (2023, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SEED SYSTEMS VALUE CHAINS POLICIES HYBRIDS MAIZE WHEAT

Impact of manures and fertilizers on yield and soil properties in a rice-wheat cropping system

Alison Laing Akbar Hossain (2023, [Artículo])

The use of chemical fertilizers under a rice-wheat cropping system (RWCS) has led to the emergence of micronutrient deficiency and decreased crop productivity. Thus, the experiment was conducted with the aim that the use of organic amendments would sustain productivity and improve the soil nutrient status under RWCS. A three-year experiment was conducted with different organic manures i.e. no manure (M0), farmyard manure@15 t ha-1 (M1), poultry manure@6 t ha-1(M2), press mud@15 t ha-1(M3), rice straw compost@6 t ha-1(M4) along with different levels of the recommended dose of fertilizer (RDF) i.e. 0% (F1), 75% (F2 and 100% (F3 in a split-plot design with three replications and plot size of 6 m x 1.2 m. Laboratory-based analysis of different soil as well as plant parameters was done using standard methodologies. The use of manures considerably improved the crop yield, macronutrients viz. nitrogen, phosphorus, potassium and micronutrients such as zinc, iron, manganese and copper, uptake in both the crops because of nutrient release from decomposed organic matter. Additionally, the increase in fertilizer dose increased these parameters. The system productivity was maximum recorded under F3M1 (13,052 kg ha-1) and results were statistically identical with F3M2 and F3M3. The significant upsurge of macro and micro-nutrients in soil and its correlation with yield outcomes was also observed through the combined use of manures as well as fertilizers. This study concluded that the use of 100% RDF integrated with organic manures, particularly farmyard manure would be a beneficial resource for increased crop yield, soil nutrient status and system productivity in RWCS in different regions of India.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ORGANIC FERTILIZERS YIELDS SOIL PROPERTIES RICE WHEAT CROPPING SYSTEMS

QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22

Ming Li Shuanghe Cao xianchun xia Zhonghu He Yong Zhang (2023, [Artículo])

KASP Markers CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA PREHARVEST SPROUTING GERMINATION INDEX QUANTITATIVE TRAIT LOCI WHEAT

Fighting food, fertilizer, and the climate crisis in Africa through targeted nitrogen management

Tek Sapkota Sieglinde Snapp (2022, [Objeto de congreso])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA FOOD INSECURITY NITROGEN FERTILIZERS RICE WHEAT MAIZE NUTRIENT MANAGEMENT