Potential of Omics to control diseases and pests in the Coconut tree

MIGUEL ALONSO TZEC SIMA Jean Wildort Félix María Inés Granados Alegría Mónica Aparicio Ortiz Dilery Juarez Monroy Damian Mayo Sarai Vivas-Lopez Rufino Gómez-Tah Blondy Beatriz Canto Canché Maxim Berezovski Ignacio Rodrigo Islas Flores (2022, [Artículo])

The coconut palm (Cocos nucifera L.) is a common crop in pantropical areas facing various challenges, one of them being the control of diseases and pests. Diseases such as bud rot caused by Phytophthora palmivora, lethal yellowing caused by phytoplasmas of the types 16SrIV-A, 16SrIV-D or 16SrIV-E, among others, and pests like the coconut palm weevil, Rhynchophorus vulneratus (Coleoptera: Curculionidae), and the horned beetle, Oryctes rhinocerus (Coleoptera: Scarabaeidae), are controlled by applying pesticides, pheromones and cultural control. These practices do not guarantee eradication since some causal agents have become resistant or are imbedded in infected tissues making them difficult to eradicate. This review condenses the current genomics, transcriptomics, proteomics and metabolomics studies which are being conducted with the aim of understanding the pathosystems associated with the coconut palm, highlighting the findings generated by omics studies that may become future targets for the control of diseases and pests in the coconut crop. © 2022 by the authors.

COCOS NUCIFERA L. OMICS PESTS INSECTS DISEASES PATHOGENS BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

Análisis de la expresión de genes involucrados en la conversión en planta de embriones somáticos de Capsicum chinense Jacq.

Gema Pijeira Fernández (2020, [Tesis de maestría])

BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR DE PLANTAS BIOLOGÍA MOLECULAR DE PLANTAS

The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Angel Alpuche-Solis CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022, [Artículo])

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

The new proletariat of the north at Ciudad Juarez: Current trends.

Nolberto Tlacaelel Acosta Pérez (2022, [Artículo, Artículo])

The arrival of the nowadays renamed Manufacturing, Maquiladora and Export Service Industry brought within the formation of a new labor subject in the region: the new proletariat of the north. The proposal of the present article is to identify and describe the specific traits of this working class sub-fraction, by a reevaluation of its meaning, the most relevant socioeconomic indicators which serve to understand it, the political organization chapters that it has undertaken in the past years, and its most characteristic cultural attributes.

new proletariat of the north maquiladora U.S.-Mexico border social classes Ciudad Juarez nuevo proletariado del norte frontera norte clases sociales Ciudad Juárez CIENCIAS SOCIALES CIENCIAS SOCIALES

Habitability conditions for floods: the case of Tuxtla Gutiérrez, Chiapas, Mexico

MARCELINO GARCIA BENITEZ Saul Nucamendi Hernandez Omar Ávila Flores (2022, [Artículo, Artículo])

At present, there are changes in the environment that favor the presence of extreme climatic phenomena such as floods, this exposure implies that the populations of urban areas suffer effects on their material assets, which limits their capacity for individual and / or family development. Given the lack of local territorial planning, the establishment of colonies was allowed on the banks of the Sabinal river and its tributaries that present episodes of flooding during the rainy season (Sistema Municipal de Protección Civil, 2015).

 

The study evaluates the habitability conditions of the urban population exposed to flooding, the city of Tuxtla Gutiérrez, Chiapas is taken as a reference, since historically it is a recurring problem during the summer. The methodology interrelates geostatistical, spatial and cartographic databases with hemerographic information sources based on the review of the literature for the construction of the general index, determined in dimensions that group social, economic, educational aspects, availability of basic services, goods with those that count the dwellings and their accessibility, etc., on a geographical scale of Urban Geostatistical Areas (AGEB).

 

The results obtained generated an index on the distribution of habitability conditions by urban Ageb, they are interrelated with spatial data on floods and their relationship with Sustainable Development Goal 11 (ODS), which proposes to improve infrastructure conditions in cities and towns. sustainable communities so that the inequality originated among the population exposed to future extreme flood events that occur in the different areas that make up the city is reduced.

Habitability conditions floods Sustainable Development Goals urban Ageb city of Tuxtla Gutiérrez, Chiapas AGEB urbana ciudad de Tuxtla Gutiérrez, Chiapas condiciones de habitabilidad inundaciones CIENCIAS SOCIALES CIENCIAS SOCIALES

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Design and finite element analysis of a 3D-printed packaging insert

Diseño y análisis de elemento finito de un inserto paraempaque manufacturado por impresión 3D

Ismael Alejandro Muñoz Salazar ISAIAS EMMANUEL GARDUÑO OLVERA MAYRA DEL ANGEL MONROY (2023, [Artículo])

Packaging inserts play a crucial role in protecting products during transportation. However, their design and production processes often rely on conventional methods limiting equipment capabilities. Moreover, the empirical nature of their design can result in a lack of reliability in the final product. To address these challenges, this study aimed to validate the design of a packaging insert using the finite element method and subsequently create it using 3D printing. The chosen material is a thermoplastic polyurethane (TPU) filament commonly used in fused deposition filament printers for 3D printing. This process demonstrates the feasibility of using 3D printing to create cushioning inserts for packaging and employing finite element analysis to simulate the insert behavior. The main findings of this research highlight the potential benefits of numerical simulation, revealing the areas where the insert is primarily impacted by weight. Furthermore, the forces load and displacement simulation results confirm that the TPU elastic limit (3.9x106 MPa) is sufficient to handle the weight this insert intends to hold. These tools determine the viability of the proposed design for its intended application. Therefore, this study verifies that 3D printing is a reliable option for producing packaging inserts, offering significant advantages over traditional methods. These advantages include increased design flexibility and the ability to create custom inserts on demand.

Los insertos de empaque juegan un papel crucial en la protección de los productos durante el transporte. Sin embargo, sus procesos de diseño y producción a menudo se basan en métodos convencionales que limitan las capacidades del equipo. Además, la naturaleza empírica de su diseño puede resultar en una falta de confiabilidad en el producto final. Para abordar estos desafíos, este estudio tuvo como objetivo validar el diseño de un inserto de empaque utilizando el método de elementos finitos y posteriormente crearlo mediante impresión 3D. El material elegido es un filamento de poliuretano termoplástico (TPU) comúnmente utilizado en impresoras de filamento de deposición fundida para impresión 3D. Este proceso demuestra la viabilidad de utilizar la impresión 3D para crear insertos acolchados para empaques y emplear el análisis de elementos finitos para simular el comportamiento del inserto. Los principales hallazgos de esta investigación destacan los beneficios potenciales de la simulación numérica, revelando las áreas donde el inserto se ve afectado principalmente por el peso. Además, los resultados de la simulación de carga y desplazamiento de fuerzas confirman que el límite elástico de TPU (3.9x106 MPa) es suficiente para manejar el peso que este inserto pretende soportar. Estas herramientas determinan la viabilidad del diseño propuesto para su aplicación prevista. Por lo tanto, este estudio verifica que la impresión 3D es una opción confiable para producir insertos de empaque, que ofrece ventajas significativas sobre los métodos tradicionales. Estas ventajas incluyen una mayor flexibilidad de diseño y la capacidad de crear insertos personalizados bajo demanda.

Tipo de documento: cases studies.

I. Muñoz gratefully acknowledges support from Posgrado de CIATEQ. I. E. Garduño acknowledges support from the Investigadores por México - CONAHCYT program through project No. 674.

Ismael Muñoz: Conceptualization; methodology; validation; writing; revision; project administration; software; investigation; display; draft writing; reviewing and editing. Isaías E. Garduño: Conceptualization; methodology; validation; writing; revision; software; reviewing and editing. Mayra del Ángel Monroy: Conceptualization; supervision; methodology; validation; writing; revision; project management; formal analysis, draft writing: writing reviewing and editing.

Packaging insert Finite element analysis 3D printing Inserto para empaque Análisis por elemento finito Impresión 3D INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

Una articulación fundamental: la emancipación

Nicolás Amoroso Boelcke (2023, [Capítulo de libro])

El concepto, la circunstancia de la enseñanza que aquí abordaremos, tiene el propósito de restituir ciertas situaciones que corresponden al propio ejercicio de la instrucción y a aquellas otras que hablan del acontecer, por ello el trabajo. Tiene las variables tanto de la experiencia real como de las otras sobre las que se han escrito, una de ellas, al final, plantea una situación paradójica que se refiere a la del maestro ignorante que, básicamente, tiene como propósito mostrar que el conocimiento es algo que está en el alumno y que el docente debe limitarse a tratar de que aflore y se exprese de la mejor manera posible. Nos habla de la independencia y de las características que puede tener una educación rigurosa y rígida, en contraposición con una más flexible. Lo mismo sucede con el pensamiento desde nuestra América, de la educación dependiente a una educación libertaria.

The concept, the circumstance of the teaching that we will address here has the purpose of restoring certain situations that correspond to the exercise itself as those others that speak of the happening for it the work. It has the variables of real experience as of the others about which they have been written, one of them, in the end, poses a paradoxical situation that refers to that of the ignorant teacher that, basically, has the purpose of showing that knowledge is something that is in the student and that the teacher must limit himself to trying to bring it out and express itself in the best possible way. It tells us about independence and the characteristics that a rigorous and rigid education can have with a more flexible one and the same happens with the thought from our America, from dependent education to a libertarian education.

Emancipación, docencia, alumnos, investigación, juego. Emancipation, teaching, students, research, play. Interaction analysis in education. Microteaching. Teacher-student relationships. Transformative learning. Educational innovations. Análisis de interacción en la educación. Relaciones maestro-estudiante. Aprendizaje transformador. Cambio educativo. LB1034 HUMANIDADES Y CIENCIAS DE LA CONDUCTA PEDAGOGÍA TEORÍA Y MÉTODOS EDUCATIVOS

Development of a next generation SNP genotyping array for wheat

Simon Griffiths Susanne Dreisigacker Alison Bentley Gina Brown-Guedira (2023, [Artículo])

Axiom Array High Throughput Genotyping Hexaploid Wheat Copy Number Variation Analysis CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SINGLE NUCLEOTIDE POLYMORPHISMS WHEAT GENOTYPING GENETIC DIVERSITY (AS RESOURCE)

Low nitrogen narrows down phenotypic diversity in durum wheat

Tesfaye Geleta Aga Bekele Abeyo (2023, [Artículo])

Clusters Durum Wheat Nitrogen Efficiency CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA HARD WHEAT GENETIC DIVERGENCE NORMALIZED DIFFERENCE VEGETATION INDEX NITROGEN PRINCIPAL COMPONENT ANALYSIS