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ANA RUTH ALVAREZ SANCHEZ CLAUDIO HUMBERTO MEJIA RUIZ Héctor Gerardo Nolasco Soria Alberto Peña Rodríguez (2018)
"Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding and nutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and high content of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y. lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed onwhiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced optical density of a yeast suspension. Digestion was –0.00236 ± 0.00010 OD U min–1 for intact cells and –0.00325 ± 0.00010 OD U min–1 for lysed cells. Release of reducing sugars in intact cells (5.3940 ± 0.1713 μmol h–1), and lysed cells (0.8396 ± 0.2251 μmol h–1) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control. Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged. Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp."
Article
Marine yeast, cell digestibility, cell viability, turbidimetry, reduced sugars BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOQUÍMICA BIOQUÍMICA DE ALIMENTOS BIOQUÍMICA DE ALIMENTOS
Francois Tardieu (2007)
Article
Environmental Stimuli Expansins CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CELLS CROPS GENETIC ENGINEERING PROTEINS TISSUE EPIDERMIS ZEA MAYS
URIEL ALEJANDRO LOPEZ LEMUS RAQUEL GARZA GUAJARDO ORALIA BARBOZA QUINTANA ALEJANDRINA RODRIGUEZ HERNANDEZ José Guzmán Esquivel ALEJANDRO GARCÍA RIVERA VIOLETA M. MADRIGAL PÉREZ LAURA ELVIRA GARCÍA LABASTIDA ALEJANDRO DAVID SORIANO HERNANDEZ MARGARITA DE LA LUZ MARTINEZ FIERRO IRAM PABLO RODRIGUEZ SANCHEZ ELIZABETH SANCHEZ DUARTE ARIANA CABRERA LICONA GABRIEL CEJA ESPIRITU IVAN DELGADO ENCISO (2018)
Metabolic syndrome (MS) has been associated with testicular damage. Nonalcoholic fatty liver disease (NAFLD) is a multisystemic disease that affects different organs, but its effect on the testes is unknown. A study analyzing germ cell involvement on BALB/c mice was carried out. A parallel comparative study was conducted that investigated alterations in the germinal epithelium of male humans that died from an unrelated acute event. The complete medical histories and histologic samples of the thoracic aorta, liver tissue, and testicular tissue from the deceased subjects were collected. The degree of germinal epithelial loss (DGEL) was evaluated and the clinical and histologic data were compared between individuals with and without NAFLD. The only metabolic or morphologic variable that caused a significant difference in the DGEL, in both the animal model and humans, was the presence of liver steatosis. The percentage of steatosis was also correlated with the percentage of the DGEL. In humans, steatosis (greater than 20%) increased the risk 12-fold for presenting with a severe DGEL (OR: 12.5; 95% CI [1.2, 128.9]; p = .03). There was no association with age above 50 years or MS components. Steatosis grade was also correlated with atherosclerosis grade. NAFLD was a strongly associated factor implicated in severe DGEL, as well as the testis was identified as a probable target organ for damage caused by the disease. his finding could result in the search for new approach strategies in the management of men with fertility problems. Further studies are required to confirm these results.
Article
MEDICINA Y CIENCIAS DE LA SALUD Germ cells Atherosclerosis Testis Metabolic syndrome
Thermal and mechanical properties of PLA-based multiscale cellulosic biocomposites
MIGUEL ANGEL RUZ CRUZ Pedro Jesús Herrera Franco Emmanuel Alejandro Flores Johnson MARIA VERONICA MORENO CHULIM LUCIANO MIGUEL GALERA MANZANO Alex Valadez González (2022)
In this work polylactic acid (PLA) based multiscale cellulosic biocomposites were prepared with the aim to evaluate the effect of the incorporation of cellulose nanocrystals (CNCs) on the PLA biocomposites reinforced with cellulose microfibers (MFCs). For this, PLA composite materials reinforced with both MFCs and with a combination of MFCs and CNCs were prepared, while keeping the content of cellulosic reinforcements constant. The thermal and mechanical properties of these multiscale PLA biocomposites were characterized by thermogravimetry (TGA), differential scanning calorimetry (DSC), flexural mechanical and, dynamic mechanical (DMA) tests. Likewise, they were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The results show that the replacement of MFCs by CNCs in the 1–5% range appreciably modifies the thermal and mechanical properties of multiscale compounds. For example, they increase the thermal stability of the materials, modify the PLA crystallization process and play the role of adhesion promoters since the mechanical properties in flexure increase in the order of 40% and the storage modulus increases in the order of 35% at room temperature. Also, the addition of CNCs increases the relaxation temperature of the material from 50 to 60 °C, thereby expanding the temperature range for its use. © 2022 The Author(s)
Article
MULTISCALE BIOCOMPOSITES CELLULOSE MICROFIBER CELLULOSE NANOCRYSTALS HIERARCHICAL STRUCTURE PROPERTIES INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE MATERIALES PROPIEDADES DE LOS MATERIALES PROPIEDADES DE LOS MATERIALES
Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022)
Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Article
AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS
The search for cryptic L-Rhamnosyltransferases on the Sporothrix schenckii genome
Hector M. Mora-Montes Karina García-Gutiérrez Laura Cristina García Carnero Nancy Lozoya-Perez Jorge Humberto Ramírez Prado (2022)
The fungal cell wall is an attractive structure to look for new antifungal drug targets and for understanding the host-fungus interaction. Sporothrix schenckii is one of the main causative agents of both human and animal sporotrichosis and currently is the species most studied of the Sporothrix genus. The cell wall of this organism has been previously analyzed, and rhamnoconjugates are signature molecules found on the surface of both mycelia and yeast-like cells. Similar to other reactions where sugars are covalently linked to other sugars, lipids, or proteins, the rhamnosylation process in this organism is expected to involve glycosyltransferases with the ability to transfer rhamnose from a sugar donor to the acceptor molecule, i.e., rhamnosyltransferases. However, no obvious rhamnosyltransferase has thus far been identified within the S. schenckii proteome or genome. Here, using a Hidden Markov Model profile strategy, we found within the S. schenckii genome five putative genes encoding for rhamnosyltransferases. Expression analyses indicated that only two of them, named RHT1 and RHT2, were significantly expressed in yeast-like cells and during interaction with the host. These two genes were heterologously expressed in Escherichia coli, and the purified recombinant proteins showed rhamnosyltransferase activity, dependent on the presence of UDP-rhamnose as a sugar donor. To the best of our knowledge, this is the first report about rhamnosyltransferases in S. schenckii. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Article
FUNGAL CELL-WALL GLYCANS RHAMNOCONJUGATES RHAMNOSYLTRANSFERASE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA VEGETAL (BOTÁNICA) ECOLOGÍA VEGETAL ECOLOGÍA VEGETAL
Hacia el desarrollo de un nuevo antiparasitario basado en células en suspensión de Carica papaya
CYNTHIA ALEJANDRA GUZMÁN MEDINA (2023)
Las enfermedades parasitarias son un problema de salud pública que afectan a más de la
quinta parte de la población a nivel mundial. Además de la morbilidad que generan, sino
son tratadas y controladas de manera adecuada pueden llegar a causar la muerte.
En el mercado existen diferentes antiparasitarios comerciales, que si bien son efectivos,
presentan diferentes efectos adversos tanto para el individuo parasitado (i.e. destrucción
de la microbiota intestinal) como para el medio ambiente. Este panorama señala la
relevancia de desarrollar tratamientos antiparasitarios más amigables con el ambiente y con
menores efectos adversos que estén disponibles actualmente en el mercado. Productos
obtenidos a partir de plantas con propiedades anti-parasitarias, cultivadas in vitro en
condiciones controladas, podrían representar una alternativa realista. Entre ellas figura la
planta Carica papaya, especie de zonas tropicales y subtropicales del planeta a cuyos frutos
se les adjudica actividades antiparasitarias. Estas propiedades se atribuyen a algunos de
sus compuestos concentrados en el latex y en las semillas como la papaina, la
quimiopapaína, la lisozima, la glicil-endopeptidasa, la proteasa de cisteína y el benzil-
isotiocinato.
El objetivo del presente trabajo fue evaluar la actividad antiparasitaria in vitro e in vivo de
un conjunto de callos y líneas de células de papaya de Carica papaya contra cisticercos de
Taenia crassiceps (cestodo) y contra trofozoitos de Entamoeba histolytica (protozoario). Los
extractos acuosos obtenidos a partir de callos y células cultivados en placa o en suspensión,
respectivamente, de las diferentes líneas de papaya revelaron alta capacidad cisticida
(>98% de mortalidad) in vitro contra cisticercos de Taenia crassiceps. En la evaluación in
vivo la línea no transformada obtuvo mayor efecto cisticida a 10mg/mL reduciendo el
número de cisticercos gemantes y aumentando los cisticercos calcificados en niveles
similares a los obtenidos utilizando antiparasitarios comerciales (albendazol y niclosamida).
Las líneas transformadas y sin transformar de papaya evaluadas indujeron una alta
capacidad amebicida (97% de mortalidad) in vitro contra trofozoitos de Entamoeba
histolytica. Las diferentes líneas transformadas y sin transformar de papaya redujeron
significativamente el número de abscesos amebianos y previnieron la hepatomegalia de
manera no significativamente diferente que el anti-parasitario comercial (metronidazol).
Los resultados generados en el presente estudio señalan al extracto acuoso obtenido a
partir de cultivos en suspensión de células de papaya transformada y no transformada
indujeron alto nivel de protección in vitro e in vivo por lo tanto; este es un producto efectivo
para el tratamiento de la cisticercosis y la amebiasis.
Parasitic diseases are a public health problem that affect more than a fifth of the population
worldwide. Additionally, and along with the morbidity the produce, they can cause death if
they are not treated and controlled adequately.
There are different commercial antiparasitic on the market which, although effective,
present different adverse effects both for the individual (i.e., destruction of the intestinal
microbiota) and for the environment alike. This panorama points out the relevance of
developing more environmentally friendly antiparasitic treatments with fewer adverse effects
than the ones currently available on the market. Products obtained from plants with anti-
parasitic properties, grown in vitro under controlled conditions, could represent a realistic
alternative. Among them is the Carica papaya, a species from tropical and subtropical areas
of the planet whose fruits are believed to have antiparasitic activities. These properties are attributed to some of its compounds concentrated in the latex and seeds such as papain,
chymopapain, lysozyme, glycyl-endopeptidase, cysteine-proteinase and benzyl-
isothiocyanate.
The objective of the present work is to evaluate the in vitro and in vivo antiparasitic activity
of a set of papaya callus and cell lines from Carica papaya against cysticerci of Taenia
crassiceps (cestode) and against trophozoites of Entamoeba histolytica (protozoan). The
aqueous extracts obtained from callus and cells cultured in plates or in suspension,
respectively, of the different papaya lines revealed high cysticidal capacity (>98% mortality)
in vitro against Taenia crassiceps cysticerci. Meanwhile, in the in vivo evaluation, the non-
transformed line obtained a greater cysticidal effect at 10 mg/mL, reducing the number of
budding cysticerci and increasing calcified cysticerci at levels like those obtained using
commercial antiparasitic (albendazole and niclosamide). The papaya lines evaluated
induced a high amoebicidal capacity (97% mortality) in vitro against Entamoeba histolytica
trophozoites. The different papaya lines significantly reduced the number of amoebic
abscesses and prevented hepatomegaly in a non-significantly different manner than the
commercial anti-parasitic (metronidazole).
The results produced in this thesis indicate that the aqueous extract obtained from
suspension cultures of transformed and non-transformed papaya cells induced a high level
of protection in vitro and in vivo. Therefore, this may represent an effective product for the
treatment of cysticercosis and amoebiasis.
Doctoral thesis
CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS Carica papaya, antiparasitario, células en suspensión, T. crassiceps, E. histolytica. Carica papaya, antiparasitic, in-suspension cells, T. crassiceps, E. histolytica.
Sonia Quijano (2020)
Pseudo-nitzschia is a cosmopolitan genus, some species of which can produce domoic acid (DA), a neurotoxin responsible for the Amnesic Shellfish Poisoning (ASP). In this study, we identified P. subpacifica for the first time in Todos Santos Bay and Manzanillo Bay, in the Mexican Pacific using SEM and molecular methods. Isolates from Todos Santos Bay were cultivated under conditions of phosphate sufficiency and deficiency at 16°C and 22°C to evaluate the production of DA. This toxin was detected in the particulate (DAp) and dissolved (DAd) fractions of the cultures during the exponential and stationary phases of growth of the cultures. The highest DA concentration was detected during the exponential phase grown in cells maintained in P-deficient medium at 16°C (1.14 ± 0.08 ng mL-1 DAd and 4.71 ± 1.11 × 10−5 ng cell-1 of DAp). In P-sufficient cultures DA was higher in cells maintained at 16°C (0.25 ± 0.05 ng mL-1 DAd and 9.41 ± 1.23 × 10−7 ng cell-1 of DAp) than in cells cultured at 22°C. Therefore, we confirm that P. subpacifica can produce DA, especially under P-limited conditions that could be associated with extraordinary oceanographic events such as the 2013–2016 "Blob" in the northeastern Pacific Ocean. This event altered local oceanographic conditions and possibly generated the presence of potential harmful species in areas with economic importance on the Mexican Pacific coast. © 2020 Quijano-Scheggia et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Article
domoic acid, domoic acid, kainic acid, Article, cell growth, controlled study, diatom, Mexico, morphology, nonhuman, Pacific Ocean, phylogeny, plant cell, plant growth, Pseudo nitzschia, toxin analysis, cell culture technique, classification, diatom, CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA
Maraeva Gianella Daniele Dondi Andreas Börner Anca Macovei Andrea Pagano Filippo Guzzon Alma Balestrazzi (2022)
Article
Thermogravimetry CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CARBOHYDRATES PROLINE TOCOPHEROLS GENETICS PHYSIOLOGY PEAS SEEDS SUGARS DIFFERENTIAL SCANNING CALORIMETRY SEED LONGEVITY THERMOGRAVIMETRIC ANALYSIS
ELIANA VALENCIA LOZANO LISSET HERRERA ISIDRON Osiel Salvador Recoder-Meléndez Aarón Barraza Celis JOSE LUIS CABRERA PONCE (2022)
"Potato microtuber (MT) development through in vitro techniques are ideal propagules for producing high quality potato plants. MT formation is influenced by several factors, i.e., photoperiod, sucrose, hormones, and osmotic stress. We have previously developed a protocol of MT induction in medium with sucrose (8% w/v), gelrite (6g/L), and 2iP as cytokinin under darkness. To understand the molecular mechanisms involved, we performed a transcriptome-wide analysis. Here we show that 1715 up- and 1624 down-regulated genes were involved in this biological process. Through the protein–protein interaction (PPI) network analyses performed in the STRING database (v11.5), we found 299 genes tightly associated in 14 clusters. Two major clusters of up-regulated proteins fundamental for life growth and development were found: 29 ribosomal proteins (RPs) interacting with 6 PEBP family members and 117 cell cycle (CC) proteins. The PPI network of up-regulated transcription factors (TFs) revealed that at least six TFs–MYB43, TSF, bZIP27, bZIP43, HAT4 and WOX9–may be involved during MTs development. The PPI network of down-regulated genes revealed a cluster of 83 proteins involved in light and photosynthesis, 110 in response to hormone, 74 in hormone mediate signaling pathway and 22 related to aging."
Article
transcriptome-wide analysis, microtubers, potato, Solanum tuberosum, darkness, cell cycle, ribosomal proteins, PEBP family genes, cytokinin BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS