Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

CARLOS ABRAHAM GUERRERO RUIZ (2019, [Artículo])

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

Recomendaciones en la creación de un portal en línea para comercializar monederos electrónicos de vales de despensa a prospectos privados

ERIK VAZQUEZ PEDROZA (2023, [Otro, Trabajo de grado, maestría])

Muchas empresas otorgan como prestación social a sus empleados monederos electrónicos de vales de despensa. La

contratación de este servicio en la mayoría de los casos se hace por medios físicos, pero la realidad es que dicha

contratación puede generarse de forma electrónica. Las empresas están cambiando su forma de contratación al medio digital, e incluso los clientes en algunos casos buscan empresas que les ahorren tiempo en procesos. Da un valor el facilitarle procesos a clientes, empleados, disminuyendo tiempos de contratación e incluso gastos en mensajería,

gasolina de ejecutivos y tiempo e incluso mitigar riesgos respecto a pérdida de contratos originales o daño en ellos. la realidad es que las empresas poco a poco migrarán sus procesos a nuevas tecnologías o a tecnologías disruptivas y en

muchos casos por solicitud de sus clientes quienes buscan disminuir procesos. Por medio del presente trabajo se darán

recomendaciones para poder implementar la contratación de forma electrónica analizando temas de datos personales, electrónica, derecho de los consumidores, marcas, nombres de dominio, entre otros.

Contratos Protección de datos Firma electrónica Marcas INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS TECNOLOGÍA DE LAS TELECOMUNICACIONES TECNOLOGÍA DE LAS TELECOMUNICACIONES

El lobo marino de California (Zalophus californianues) y su relación con la pesca en la región de las Grandes Islas, Golfo de California, México

Alfredo Zavala González (1999, [Tesis de doctorado])

El lobo marino de California (Z. californianus) se ha usado desde épocas prehispanicas en aguas mexicanas. En el México Prehispanico los nativos lo utilizaban en bajo nivel. Durante el México Colonial e Independiente, el mayor uso de lobos marinos fue por

The California sea lion (Zalophus californianus) has been utilized in Mexican waters for many years. During the Prehispanic Period, native people used sea lions to a very small extent. The Colonial and Independent Periods showed a higher use of sea lions

Tiburón, Golfo de California, Lobo marino de California, Ciencias del mar CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE PECES Y FAUNA SILVESTRE

Bacterial communities in the rhizosphere at different growth stages of maize cultivated in soil under conventional and conservation agricultural practices

Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Farmers in Mexico till soil intensively, remove crop residues for fodder and grow maize often in monoculture. Conservation agriculture (CA), including minimal tillage, crop residue retention and crop diversification, is proposed as a more sustainable alternative. In this study, we determined the effect of agricultural practices and the developing maize rhizosphere on soil bacterial communities. Bulk and maize (Zea mays L.) rhizosphere soil under conventional practices (CP) and CA were sampled during the vegetative, flowering and grain filling stage, and 16S rRNA metabarcoding was used to assess bacterial diversity and community structure. The functional diversity was inferred from the bacterial taxa using PICRUSt. Conservation agriculture positively affected taxonomic and functional diversity compared to CP. The agricultural practice was the most important factor in defining the structure of bacterial communities, even more so than rhizosphere and plant growth stage. The rhizosphere enriched fast growing copiotrophic bacteria, such as Rhizobiales, Sphingomonadales, Xanthomonadales, and Burkholderiales, while in the bulk soil of CP other copiotrophs were enriched, e.g., Halomonas and Bacillus. The bacterial community in the maize bulk soil resembled each other more than in the rhizosphere of CA and CP. The bacterial community structure, and taxonomic and functional diversity in the maize rhizosphere changed with maize development and the differences between the bulk soil and the rhizosphere were more accentuated when the plant aged. Although agricultural practices did not alter the effect of the rhizosphere on the soil bacterial communities in the flowering and grain filling stage, they did in the vegetative stage.

Community Assembly Functional Diversity Intensive Agricultural Practices Plant Microbiome CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SUSTAINABLE AGRICULTURE TILLAGE SOIL BACTERIA MAIZE

Influence of conservation agriculture-based production systems on bacterial diversity and soil quality in rice-wheat-greengram cropping system in eastern Indo-Gangetic Plains of India

Anup Das virender kumar Peter Craufurd Andrew Mcdonald Sonam Sherpa (2023, [Artículo])

Introduction: Conservation agriculture (CA) is gaining attention in the South Asia as an environmentally benign and sustainable food production system. The knowledge of the soil bacterial community composition along with other soil properties is essential for evaluating the CA-based management practices for achieving the soil environment sustainability and climate resilience in the rice-wheat-greengram system. The long-term effects of CA-based tillage-cum-crop establishment (TCE) methods on earthworm population, soil parameters as well as microbial diversity have not been well studied. Methods: Seven treatments (or scenarios) were laid down with the various tillage (wet, dry, or zero-tillage), establishment method (direct-or drill-seeding or transplantation) and residue management practices (mixed with the soil or kept on the soil surface). The soil samples were collected after 7 years of experimentation and analyzed for the soil quality and bacterial diversity to examine the effect of tillage-cum-crop establishment methods. Results and Discussion: Earthworm population (3.6 times), soil organic carbon (11.94%), macro (NPK) (14.50–23.57%) and micronutrients (Mn, and Cu) (13.25 and 29.57%) contents were appreciably higher under CA-based TCE methods than tillage-intensive farming practices. Significantly higher number of OTUs (1,192 ± 50) and Chao1 (1415.65 ± 14.34) values were observed in partial CA-based production system (p ≤ 0.05). Forty-two (42) bacterial phyla were identified across the scenarios, and Proteobacteria, Actinobacteria, and Firmicutes were the most dominant in all the scenarios. The CA-based scenarios harbor a high abundance of Proteobacteria (2–13%), whereas the conventional tillage-based scenarios were dominated by the bacterial phyla Acidobacteria and Chloroflexi and found statistically differed among the scenarios (p ≤ 0.05). Composition of the major phyla, i.e., Proteobacteria, Actinobacteria, and Firmicutes were associated differently with either CA or farmers-based tillage management practices. Overall, the present study indicates the importance of CA-based tillage-cum-crop establishment methods in shaping the bacterial diversity, earthworms population, soil organic carbon, and plant nutrient availability, which are crucial for sustainable agricultural production and resilience in agro-ecosystem.

Metagenomics Bacterial Diversity Rice-Wheat-Greengram CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CONSERVATION AGRICULTURE DNA SEQUENCES EARTHWORMS METAGENOMICS SOIL QUALITY AGROECOSYSTEMS

Tasas de pastoreo diarias del zooplacton en una localidad costera de Baja California, México

Ma. Eugenia del Carmen Matus Hernández (1991, [Tesis de maestría])

En una localidad costera de la Bahia de Todos Santos se efectuaron muestreosdiarios durante 15 dias enjulio de 1990. El objetivo fue evaluar las tasas de pastoreo de los herbivoros dominantes (micro y macrozooplancton) sobre la biomasafitoplanct6nica. Las

Productividad primaria,Poblaciones de fitoplancton,Baja California,Ciencias del mar CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE PECES Y FAUNA SILVESTRE

El lobo marino de California (Zalophus californianues) y su relación con la pesca en la región de las Grandes Islas, Golfo de California, México

Alfredo Zavala González (1999, [Tesis de doctorado])

El lobo marino de California (Z. californianus) se ha usado desde épocas prehispanicas en aguas mexicanas. En el México Prehispanico los nativos lo utilizaban en bajo nivel. Durante el México Colonial e Independiente, el mayor uso de lobos marinos fue por los extranjeros y era secundario a la obtencién de otros mamiferos marinos. En el México Postrevolucionario, los lobos marinos eran el blanco de diferentes usos industriales. El uso de lobo marino se termind oficialmente en 1982, pero todavia se le caza a una baja escala, de manera irregular. Entre 1992 y 1997 la poblacién del lobo marino en la Region de las GrandesIslas del Golfo de California se mantuvo estable, alrededor de los 9,200 animales. No se evidencia un cambio importante en el tamafio de la poblacion durante los ultimos 55 afos; sin embargo, se observa un fuerte decremento en la poblacién entre 1990-93. Esto fue coincidente con una disminucién en los pelagicos menores y puede reflejar una relacion entre escalas decadales y cambios climaticos mayoresy la estabilidad de la poblacion del lobo marino en el Golfo de California. La pesca tiene lugar todo el afio en todas las aguas adyacentes al archipiélago Angel de la Guarda, pero hay sitios con mayor actividad. En Bahia de Los Angeles se presenta una afluencia de pescadores foraneos que triplica la poblacién pesquera local durante la temporada de pesca de tibur6n. El numero de equipos de pesca y la duracién de los campamentos responde a la abundancia y demanda de los recursos. Se observa una alta eficiencia en la pesca riberefa para las capturas multiespecificas, con gran variedad de especies neriticas, pelagicas y demersales, y con poco desperdicio de fauna de acompafhamiento. El area de pesca se puede incrementar ante condiciones anomalas, como durante eventos del ENSO, pero también se observa un incremento en el uso de nuevas zonas de pesca en los ultimos afios. Los pescadoreslocales accidentalmente capturan lobos marinos en sus redes. Matan a estos lobos enmallados para poder desatorarlos y recuperar sus pafios, por el peligro que existe al tratar de liberarlos vivos. La tasa de enmallamiento registrada en el presente trabajo (0.49%) no presenta una amenazapara la conservacion de la especie en el la region centro-norte del Golfo de California. La pesca de tibur6n a gran escala desarrollada por pescadores foraneos, a diferencia de la pesca local (de menores dimensiones en términos de volumen, esfuerzo e inversion), causa mayores problemasa los recursos marinos(incluyendo tortugas marinas, delfines, pélagicos mayores y lobos marinos, entre otras). Esta hace uso ademas de una matanza deliberada de lobos inmaduros como carnada. Para este tipo de pescadores, mientras siga “disponible” el acceso a los lobos marinos, seran inevitables sus capturas para carnada. Se concluye que: 1) la poblacién de la especie en la Region de las Grandes Islas no se ncuentra reducida ni amenazada; 2) la interaccién del lobo con la pesca riberefia de escama, actualmente se encuentra aniveles no preocupantes, pero habra que observarel incremento en el esfuerzo pesquero; 3) la interaccién con la pesca especializadade tiburon, a gran escala, puede al corto plazo representar problemas para algunas colonias de lobos marinos; 4) se plantea que la especie puede ser considerada comorecurso y se proyecte su utilizacién controlada y normada para beneficio de la poblaciénlocal.

The California sea lion (Zalophus californianus) has been utilized in Mexican waters for many years. During the Prehispanic Period, native people used sea lions to a very small extent. The Colonial and Independent Periods showed a higher use of sea lions by foreigners and it was secondary to the extraction of other marine mammals. During the Postrevolutionary era, sea lions becamethetarget for industrial use. Sea lion use officially ended in 1982, but its irregular huntisstill seen today at a low level. From 1992 to 1997 the sea lion population in the Gulf of California Great Islands Region was stable, at around 9,200 animals. There is no evidence of an important changein the size of the population over the last 55 year; however, a strong decline in:the population between 1990 and 1993 is apparent. This coincides with a reduction in minor pelagics and could reflect a relationship between decadal scales and majorclimatic changes and the stability of the sea lion population in the Gulf of California. Fishing occurs all year round in the waters Surrounding the Angel de la Guarda archipielago, but there are several sites where there is more activity. The community of Bahia de los Angeles shows the presence of non-local fishermen, tripling the local fishermen population. The number of fishing teams and the duration of the camps depends on resource abundance and demand. High efficiency is shownin artisanalfishery for multispecies catches, with a great variety of neritic, pelagic and demersal species and very low waste of accompanying fauna. The area fished can increase with unusual conditions, such as during ENSO events, but recent years have also exhibited a rise in the use of new fishing zones. Local fishermen accidentally capture sea lions in their nets. They kill the entangled animalsin order to release them from their fishing gear, so they can repair their nets and because of the dangeroftrying to free them alive. The entanglement ratio (0.49%) does not represent a threat to the species conservation in the north central region of the Gulf of California. The shark fishing developed to a great extent by outside fishermen, as opposedto local fishing (of smaller dimensions in terms of volume and investment), causes major problems to natural resources such as marine turtles, dolphins, pelagic species and sealions.Immature sealions are also killed deliberately for use as bait. For these fishermen, as long as accessto sealions is available, their capture for bait is inevitable. It is concluded that: 1) the population of the species in the Great Islands Regionis not found to be reduced or threatened; 2) sea lion interaction with artisanal fishing is not currently found to be at a level to cause concern, but the increasein the fishing effort needs to be observed; 3) the interaction with specialised shark fishing could represent problems for some sea lion colonies in the short term; 4) it is stated that the species could be considered as a resource and its controlled and regulated use is suggested for the benefit of the local community.

Tiburón,Golfo de California,Lobo marino de California,Ciencias del mar CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS PECES Y FAUNA SILVESTRE PECES Y FAUNA SILVESTRE

Datación de sedimentos recientes utilizando isótopos radioactivos en el río Verde, en el estado de Oaxaca, México

Dating of recent sediments using radioactive isotopes in the Rio Verde, Oaxaca state, Mexico

José Alfredo González Verdugo EDITH ROSALBA SALCEDO SANCHEZ MARIA JOSELINA CLEMENCIA ESPINOZA AYALA MANUEL MARTINEZ MORALES (2013, [Artículo])

Con objeto de conocer la evolución de los procesos sedimentarios en la parte baja del río Verde, Oaxaca, se realizaron mediciones en dos núcleos de sedimentos. El fechado y determinación de la tasa de sedimentación, se realizó utilizando los isótopos radioactivos Plomo-210 y Cesio-137, que sirven como indicadores de períodos de deposición de los últimos 100 años. La velocidad de sedimentación en el Río Verde obtenida por medio del isótopo Pb -210 es 0.69 a 0.89 cm/año. Por otro lado, la velocidad de sedimentación en la zona de estudio mediante el isótopo Cs-137 es del orden de 0.61 cm/año y 0.87 cm/año. El método de Cesio-137 proporciona marcadores distintivos de eventos, mientras que el método de Plomo-210 proporciona pendientes de concentración, que al aplicarse de manera conjunta proporcionan una validación de ambos métodos. En este estudio, los dos métodos coinciden en los valores de sedimentación para la zona del Río Verde. Los resultados de este trabajo permiten conocer la dinámica de los procesos de transporte de sedimentos en la zona, información que resulta útil para la planeación de obras hidráulicas y estimación de los impactos ambientales, así como la implementación de las medidas de mitigación correspondientes.

Sedimentación Datación Isótopos radiactivos Marcadores Morfología de ríos CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA

Nitrogen fertilizer application alters the root endophyte bacterial microbiome in maize plants, but not in the stem or rhizosphere soil

Alejandra Miranda Carrazco Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Plant-associated microorganisms that affect plant development, their composition, and their functionality are determined by the host, soil conditions, and agricultural practices. How agricultural practices affect the rhizosphere microbiome has been well studied, but less is known about how they might affect plant endophytes. In this study, the metagenomic DNA from the rhizosphere and endophyte communities of root and stem of maize plants was extracted and sequenced with the “diversity arrays technology sequencing,” while the bacterial community and functionality (organized by subsystems from general to specific functions) were investigated in crops cultivated with or without tillage and with or without N fertilizer application. Tillage had a small significant effect on the bacterial community in the rhizosphere, but N fertilizer had a highly significant effect on the roots, but not on the rhizosphere or stem. The relative abundance of many bacterial species was significantly different in the roots and stem of fertilized maize plants, but not in the unfertilized ones. The abundance of N cycle genes was affected by N fertilization application, most accentuated in the roots. How these changes in bacterial composition and N genes composition might affect plant development or crop yields has still to be unraveled.

Bacterial Community Structure DArT-Seq Bacterial Community Functionality Genes Involved in N Cycling CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURAL PRACTICES MAIZE RHIZOSPHERE STEMS NITROGEN FERTILIZERS

Exploring the genetic diversity and population structure of wheat landrace population conserved at ICARDA genebank

Muhammad Massub Tehseen Fatma Aykut Tonk Ahmed Amri Carolina Sansaloni Ezgi Kurtulus Muhammad Salman Mubarik Kumarse Nazari (2022, [Artículo])

Wheat Landraces Genetic Diversity SNP Markers Analysis of Molecular Variance AMOVA CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA BREEDING DISCRIMINANT ANALYSIS GENETIC VARIATION GENETIC DISTANCE GENETIC IMPROVEMENT GENETIC MARKERS HEXAPLOIDY LANDRACES POPULATION STRUCTURE SINGLE NUCLEOTIDE POLYMORPHISM TRITICUM AESTIVUM WHEAT