The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Angel Alpuche-Solis CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022)

WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.

Article

BANANA TRANSCRIPTION FACTOR WRKY DEFENSE PHYTOHORMONES SALICYLIC ACID METHYL JASMONATE SAR ISR BROAD-SPECTRUM RESISTANCE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

The Banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 Genes Encode Functional Transcription Factors and Display Differential Expression in Response to Defense Phytohormones

SERGIO GARCIA LAYNES VIRGINIA AURORA HERRERA VALENCIA Lilia Guadalupe Tamayo Torres VERONICA LIMONES BRIONES FELIPE ALONSO BARREDO POOL FRAY MARTIN BAAS ESPINOLA Ángel Gabriel Alpuche Solís CARLOS ALBERTO PUCH HAU SANTY PERAZA ECHEVERRIA (2022)

"WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop."

Article

Banana Transcription factor WRKY Defense phytohormones Salicylic acid Methyl jasmonate SAR ISR Broad-spectrum resistance BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA

New genomic regions identified for resistance to spot blotch and terminal heat stress in an interspecific population of triticum aestivum and T. spelta

Sudhir Navathe Ramesh Chand Mir Asif Iquebal Govindan Velu arun joshi (2022)

Article

Resistance Terminal Heat CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA BIPOLARIS SOROKINIANA HEAT STRESS WHEAT RESISTANCE VARIETIES

First detection of virulence in Puccinia striiformis f. Sp. tritici to wheat resistance genes Yr10 and Yr24 (=Yr26) in Mexico

JULIO HUERTA_ESPINO Ravi Singh (2017)

Article

Near Isogenic Lines Virulence Spectrum Yellow Rust CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA VIRULENCE PUCCINIA STRIIFORMIS WHEAT RESISTANCE GENES

Genética de la resistencia al complejo mancha de asfalto en 18 genotipos tropicales de maíz

George Mahuku Ignacio Benítez-Riquelme Serafin Cruz-Izquierdo (2015)

Article

Horizontal Resistance Tar Spot Complex CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA FUNGI MONOGRAPHELLA PHYLLACHORALES ZEA MAYS DIALLEL ANALYSIS DISEASE RESISTANCE

Genome-wide association analysis of tan spot disease resistance in durum wheat accessions from Tunisia

marwa laribi Sarrah Ben M'barek Carolina Sansaloni Susanne Dreisigacker (2023)

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA DISEASE RESISTANCE HARD WHEAT GENETIC DIVERSITY GENOME-WIDE ASSOCIATION STUDIES LANDRACES POPULATION STRUCTURE

Resistencia a afidos en trigo

Leonardo Abdiel Crespo Herrera (2022)

Conference object

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA WHEAT PESTS CLIMATE CHANGE APHIDOIDEA DISEASE RESISTANCE

Phenotyping for Disease Resistance to increase genetic gains

Suresh L.M. (2022)

Conference object

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE PLANT DISEASES DISEASE RESISTANCE PHENOTYPES

Inheritance of resistance to Fusarium verticillioides ear rot in maize inbred lines of southern, West and Central Africa origin

Abebe Menkir Cosmos Magorokosho Maryke Labuschagne (2022)

Article

Ear-Rotting Fungi Artificial Inoculation CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE FUSARIUM PEST RESISTANCE BREEDING PROGRAMMES FUMONISINS

Gene editing to accelerate crop breeding

Kanwarpal Dhugga (2022)

Article

Accelerated Breeding Grain Biofortification Maize Lethal Necrosis Rust Resistance Site-Directed Nuclease Scenarios CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA BREEDING BACKCROSSING DISEASE RESISTANCE GENE EDITING GRAIN BIOFORTIFICATION RUSTS