Dual function of EDTA with silver nanoparticles for root canal treatment–A novel modification

JUAN MANUEL MARTINEZ ANDRADE (2018, [Artículo])

The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ?-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 ?g/ ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT. © 2018 Martinez-Andrade et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

edetic acid, silver nanoparticle, edetic acid, metal nanoparticle, silver, antibacterial activity, antibiotic sensitivity, antifungal activity, antimicrobial activity, Article, atomic absorption spectrometry, atomic force microscopy, biofilm, bovine, BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA MICROBIOLOGÍA MICROBIOLOGÍA

Chronic toxicity of shrimp feed added with silver nanoparticles (Argovit-4®) in Litopenaeus vannamei and immune response to white spot syndrome virus infection

Carlos Rosendo Romo Quiñonez Píndaro Álvarez Ruiz CLAUDIO HUMBERTO MEJIA RUIZ NINA BOGDANCHIKOVA Alexey Pestryakov CARINA GAMEZ JIMENEZ WENCESLAO VALENZUELA QUIÑONEZ Magnolia Montoya Mejía EUSEBIO NAVA PEREZ (2022, [Artículo])

"In recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4 R ) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feed-AgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feed-AgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated."

Silver nanoparticles, Shrimp, Aquaculture, Chronic toxicity, WSSV, AgNP, Argovit, Litopenaeus vannamei , Silver fed, White spot syndrome virus BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA INMUNOLOGÍA INMUNIZACIÓN INMUNIZACIÓN

Enhancement of antibiotics antimicrobial activity due to the silver nanoparticles impact on the cell membrane

Roberto Vazquez-Munoz (2019, [Artículo])

The ability of microorganisms to generate resistance outcompetes with the generation of new and efficient antibiotics; therefore, it is critical to develop novel antibiotic agents and treatments to control bacterial infections. An alternative to this worldwide problem is the use of nanomaterials with antimicrobial properties. Silver nanoparticles (AgNPs) have been extensively studied due to their antimicrobial effect in different organisms. In this work, the synergistic antimicrobial effect of AgNPs and conventional antibiotics was assessed in Gram-positive and Gram-negative bacteria. AgNPs minimal inhibitory concentration was 10–12 μg mL-1 in all bacterial strains tested, regardless of their different susceptibility against antibiotics. Interestingly, a synergistic antimicrobial effect was observed when combining AgNPs and kanamycin according to the fractional inhibitory concentration index, FICI: <0.5), an additive effect by combining AgNPs and chloramphenicol (FICI: 0.5 to 1), whereas no effect was found with AgNPs and β-lactam antibiotics combinations. Flow cytometry and TEM analysis showed that sublethal concentrations of AgNPs (6–7 μg mL-1) altered the bacterial membrane potential and caused ultrastructural damage, increasing the cell membrane permeability. No chemical interactions between AgNPs and antibiotics were detected. We propose an experimental supported mechanism of action by which combinatorial effect of antimicrobials drives synergy depending on their specific target, facilitated by membrane alterations generated by AgNPs. Our results provide a deeper understanding about the synergistic mechanism of AgNPs and antibiotics, aiming to combat antimicrobial infections efficiently, especially those by multi-drug resistant microorganisms, in order to mitigate the current crisis due to antibiotic resistance. © 2019 Vazquez-Muñoz et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ampicillin, antibiotic agent, aztreonam, beta lactam antibiotic, biapenem, chloramphenicol, kanamycin, silver nanoparticle, silver nitrate, antiinfective agent, metal nanoparticle, silver, antibiotic sensitivity, antimicrobial activity, Article, bact BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOFÍSICA BIOFÍSICA

Socialización intergeneracional en un ejido de Campeche: un continuum sociohistórico entre la migración internacional y el campo

ZULEYKA MAHITZE OLVERA YABUR (2023, [Tesis de doctorado])

“Este trabajo presenta un estudio sobre la socialización intergeneracional como un proceso continuo que impacta en la manera en que los contenidos socioculturales se transmiten, discontinúan u olvidan en una sociedad. Las personas reaccionan ante los cambios socioculturales, económicos y políticos y toman decisiones que derivan en reconfiguraciones en su modo de vida. En este estudio longitudinal, la observación de tres cohortes dio paso a una interpretación sobre qué se modifica y por qué, dando un panorama de interrelación entre los grupos de edad que completó las percepciones y perspectivas de su realidad. Sus diversas interacciones se analizaron, principalmente, desde el proceso comunicativo, por lo que la lengua tuvo un rol central en el análisis. Específicamente, está investigación muestra un recorrido sociohistórico a través de tres generaciones en Moquel –un ejido ubicado en el estado de Campeche-. Da cuenta desde el asentamiento, en 1963, de personas guanajuatenses y, posteriormente, en 1972, de familias michoacanas, que recibieron tierras por parte del gobierno federal, hasta la pandemia de Covid.-19 que fue el contexto en el que se realizó el trabajo in situ. El recorrido sociohistórico permitió abordar el estudio con base en los contenidos socioculturales que se continúan, modifican u olvidan según las adecuaciones de la sociedad, en este caso moqueleña, que delinea la transmisión de conocimientos y prácticas socioculturales a través de los procesos de socialización. Así, en este trabajo los organicé en tres ejes temáticos. El primero es la migración interna e internacional que realizan los moloqueños a Estados Unidos y que se configura, a su vez, como una tradición migratoria. Segundo, la ruralidad que engloba los espacios de socialización como la milpa, la escuela, el hogar y la iglesia donde el registro de las interacciones de las personas define ideologías, valores, creencias y costumbres que, vinculados a sus prácticas socioculturales”.

Migración e inmigración - México - Campeche. Socialización - México - Campeche. Cambio social - México - Moquel (Campeche). Familias - Aspectos económicos - México - Moquel (Campeche). Relaciones entre generaciones - México - Moquel (Campeche). Tesis - Doctorado en Antropología, CDMX. CIENCIAS SOCIALES DEMOGRAFÍA DEMOGRAFÍA GEOGRÁFICA MOVILIDAD Y MIGRACIONES INTERIORES MOVILIDAD Y MIGRACIONES INTERIORES

La autenticidad etnográfica en el trabajo de Mariángela Rodríguez Nicholls

Andrés Antonio Fábregas Puig (2023, [Artículo])

"Al leer los textos de Mariángela Rodríguez Nicholls, uno tiene ante sí la descripción etnográfica auténtica del orbe cultural creado por los inmigrantes mexicanos en Estados Unidos. Su trabajo es sólido, estructurado, sistemático y de un interés arraigado en el tema, anclado en las veredas profundas por las que transita la variedad cultural mexicana".

CIENCIAS SOCIALES DEMOGRAFÍA DEMOGRAFÍA GEOGRÁFICA MOVILIDAD Y MIGRACIONES INTERNACIONALES MOVILIDAD Y MIGRACIONES INTERNACIONALES

Advancing the science and practice of ecological nutrient management for smallholder farmers

Sieglinde Snapp (2022, [Artículo])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGROECOLOGY DEGRADATION NUTRIENT CYCLES NUTRIENT USE EFFICIENCY SOIL ORGANIC MATTER SMALLHOLDERS

Gestión electoral comparada y confianza en las elecciones en América Latina, de Fernando Barrientos del Monte

LUIS EDUARDO LEON GANATIOS (2012, [Artículo])

La gestión electoral resulta un proceso fundamental dentro de la consolidación democrática, una gestión electoral ineficiente se vería reflejada en los votantes y por ende debilitaría

la democracia.

El autor plantea una hipótesis central sobre la que gira su investigación “dependiendo el

modelo, características y desempeño del organismo electoral existente en una democracia,

aumenta o disminuye la eficiencia y eficacia en el control de la gestión electoral y sobre todo

la confianza y credibilidad política y social hacia los procesos electorales” creando así una

relación hipotética directamente proporcional a ser comprobada revisando la historia y el

desempeño institucional electoral de los países latinoamericanos,

El marco teórico de la investigación parte del estudio de cultura política de Almond y

Verba (1963), reforzado por la perspectiva analítica de Easton (1965) y ampliado y actualizado por Norris (2010) siendo un modelo que permite entrelazar las dos dimensiones estudiadas; confianza (grado de apoyo político) y desempeño institucional (grado de eficacia y

eficiencia).

CIENCIAS SOCIALES Gestión electoral Democracia

Variation in anther extrusion and its impact on fusarium head blight and deoxynivalenol content in oat (Avena sativa L.)

Xinyao He (2020, [Artículo])

Anther Extrusion Anther Retention Deoxynivalenol Fusarium Head Blight Spray Inoculation Spawn Inoculation CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA VOMITOXIN INOCULATION ANTHERS FUSARIUM

Natural disasters and economic growth: a synthesis of empirical evidence

Fernando Antonio Ignacio González (2023, [Artículo, Artículo])

Natural disasters pose a serious threat globally and, in the future, their frequency and severity are expected to increase due to climate change. Empirical evidence has reported conflicting results in terms of the impact of disasters on economic growth. In this context, the present work seeks to synthesize the recent empirical evidence related to this topic. More than 650 estimates, from studies published in the last five years (2015-2020), are used. Meta-analysis and meta-regression techniques are employed. The review includes three sources (Scopus, Science Direct, and Google Scholar). The results identified the existence of a negative and significant combined effect (-0.015). Developing countries are especially vulnerable to disasters. The negative impact is greater for disasters that occurred in the last decade -in relation to previous disasters-. These findings constitute a call for attention in favor of mitigation and adaptation policies.

Disasters GDP meta-analysis meta-regression desastres crecimiento PIB meta-análisis meta-regresión CIENCIAS SOCIALES CIENCIAS SOCIALES

Field deployable Reverse Transcriptase–Recombinase Polymerase Amplification (RT-RPA) for detection of Maize chlorotic mottle virus (MCMV)

Margaret Redinbaugh Suresh L.M. (2022, [Artículo])

Maize Lethal Necrosis Diagnostics Recombinase Polymerase Amplification CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA MAIZE MAIZE CHLOROTIC MOTTLE VIRUS DISEASES DETECTION