Efecto del polvo de romero (Rosmarinus officinalis) en el crecimiento, actividad enzimática y composición proximal de juveniles de lobina rayada (Morone saxatilis)

Effect of rosemary powder (Rosmarinus officinalis) on growth, enzyme activity, and proximal composition of juvenile stripped bass (Morone saxatilis)

Erick Adolfo Barrios García (2023, [Tesis de maestría])

La acuicultura intensiva ha experimentado un rápido crecimiento en las últimas décadas. Sin embargo, la intensificación de los cultivos acuícolas ha ocasionado problemas como el estrés en los organismos, desarrollo de enfermedades y un menor crecimiento. Se ha explorado el uso de plantas aromáticas como el romero (Rosmarinus officinalis) por su efecto como antibiótico, antiestresante y estimulante de la secreción de enzimas digestivas y el crecimiento. El objetivo de este estudio fue evaluar la adición de polvo de romero en la dieta de la lobina rayada (Morone saxatilis) y determinar su efecto en la supervivencia, crecimiento, actividad enzimática (tripsina, quimiotripsina, lipasa, amilasa y proteasas alcalinas totales -PAT-) y composición proximal. Se realizó un bioensayo durante 70 días en un sistema de recirculación de agua de mar donde se probaron cuatro dietas experimentales con diferentes cantidades de polvo de romero: control (TC), 2.5 (T2.5), 5 (T5) y 10 (T10) g kg-1 de dieta. Cada tratamiento se realizó por triplicado con 20 peces por réplica con un peso inicial de 19.59 ± 2.43 g. Las lobinas se alimentaron tres veces al día al 4% de su biomasa. Al final del bioensayo, el peso final de los peces en T5 y T10 fue significativamente menor en comparación con el TC. No se observaron diferencias significativas en parámetros de crecimiento y eficiencia alimenticia. El índice hepatosomático en el T10 fue significativamente mayor en relación con TC. En el intestino, la actividad de la tripsina fue significativamente menor en el T10 y las PAT en T2.5 y T10. En ciegos pilóricos, la actividad de la tripsina fue significativamente menor en el T10, la lipasa en T2.5, T5 y T10 y las PAT en T2.5 y T10. La inclusión de polvo de romero en la dieta de lobina en concentraciones de 5 y 10 g kg-1 disminuye el crecimiento y la actividad de tripsina, PAT y lipasa en intestino y ciegos pilóricos. Se requiere investigar la composición química del romero y su efecto como inhibidor de enzimas digestivas y modulador de la microbiota intestinal, además de incluir otros indicadores para conocer de forma integrada el desempeño fisiológico de la especie.

Intensive aquaculture has experienced a very rapid growth in recent years. However, the intensification of aquaculture has led to issues such as stress in organisms, the development of diseases, and reduced growth. The use of aromatic plants, such as rosemary (Rosmarinus officinalis), has been explored due to its antibiotic, anti-stress, and digestive enzyme secretion-stimulating effects, as well as its growth-promoting properties. The objective of this study was to evaluate the addition of rosemary powder to the diet of striped bass (Morone saxatilis) and determine its effect on survival, growth, enzyme activity (trypsin, chymotrypsin, lipase, amylase, and total alkaline proteases -PAT-), and proximate composition. A 70-day bioassay was conducted in a recirculating seawater system, testing four experimental diets with varying amounts of rosemary powder: control (TC), 2.5 (T2.5), 5 (T5), and 10 (T10) g kg-1 of diet. Each treatment was performed in triplicate with 20 fish per replicate, with an initial weight of 19.59 ± 2.43 g. The striped bass were fed three times a day at 4% of their biomass. At the end of the bioassay, the final weight of fish in T5 and T10 was significantly lower compared to TC. There were no significant differences in growth parameters and feed efficiency. The hepatosomatic index in T10 was significantly higher compared to TC. In the intestine, trypsin activity was significantly lower in T10, and PAT in T2.5 and T10. In the pyloric caeca, trypsin activity was significantly lower in T10, lipase in T2.5, T5, and T10, and PAT in T2.5 and T10. The inclusion of rosemary powder in striped bass diets at concentrations of 5 and 10 g kg-1 reduces growth and the activity of trypsin, PAT, and lipase in the intestine and pyloric caeca. Further research is imperative to delve into the intricate chemical composition of rosemary, explore its role as a potent digestive enzyme inhibitor, and its potential as a modulator of the intestinal microbiota. This endeavor should also encompass the incorporation of various additional indicators, aiming for a holistic grasp of the species' physiological performance.

lobina rayada, romero, crecimiento, enzimas digestivas, composición proximal striped bass, rosemary, growth, digestive enzymes, proximal composition CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA ACUICULTURA MARINA OCEANOGRAFÍA ACUICULTURA MARINA

Funcionamiento del proceso de digestión en rumiantes

Abel Jaime Leal González (2021, [Libro])

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA RUMIANTE DIGESTIÓN RUMINAL NUTRIENTES CARBOHIDRATOS ABOMASO ABSORCIÓN DIGESTIVA GANADO BOVINO PASTOREO RUMINANTS RUMEN DIGESTION NUTRIENTS CARBOHYDRATES ABOMASUM DIGESTIVE ABSORPTION CATTLE GRAZING

Influence of conservation agriculture-based production systems on bacterial diversity and soil quality in rice-wheat-greengram cropping system in eastern Indo-Gangetic Plains of India

Anup Das virender kumar Peter Craufurd Andrew Mcdonald Sonam Sherpa (2023, [Artículo])

Introduction: Conservation agriculture (CA) is gaining attention in the South Asia as an environmentally benign and sustainable food production system. The knowledge of the soil bacterial community composition along with other soil properties is essential for evaluating the CA-based management practices for achieving the soil environment sustainability and climate resilience in the rice-wheat-greengram system. The long-term effects of CA-based tillage-cum-crop establishment (TCE) methods on earthworm population, soil parameters as well as microbial diversity have not been well studied. Methods: Seven treatments (or scenarios) were laid down with the various tillage (wet, dry, or zero-tillage), establishment method (direct-or drill-seeding or transplantation) and residue management practices (mixed with the soil or kept on the soil surface). The soil samples were collected after 7 years of experimentation and analyzed for the soil quality and bacterial diversity to examine the effect of tillage-cum-crop establishment methods. Results and Discussion: Earthworm population (3.6 times), soil organic carbon (11.94%), macro (NPK) (14.50–23.57%) and micronutrients (Mn, and Cu) (13.25 and 29.57%) contents were appreciably higher under CA-based TCE methods than tillage-intensive farming practices. Significantly higher number of OTUs (1,192 ± 50) and Chao1 (1415.65 ± 14.34) values were observed in partial CA-based production system (p ≤ 0.05). Forty-two (42) bacterial phyla were identified across the scenarios, and Proteobacteria, Actinobacteria, and Firmicutes were the most dominant in all the scenarios. The CA-based scenarios harbor a high abundance of Proteobacteria (2–13%), whereas the conventional tillage-based scenarios were dominated by the bacterial phyla Acidobacteria and Chloroflexi and found statistically differed among the scenarios (p ≤ 0.05). Composition of the major phyla, i.e., Proteobacteria, Actinobacteria, and Firmicutes were associated differently with either CA or farmers-based tillage management practices. Overall, the present study indicates the importance of CA-based tillage-cum-crop establishment methods in shaping the bacterial diversity, earthworms population, soil organic carbon, and plant nutrient availability, which are crucial for sustainable agricultural production and resilience in agro-ecosystem.

Metagenomics Bacterial Diversity Rice-Wheat-Greengram CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CONSERVATION AGRICULTURE DNA SEQUENCES EARTHWORMS METAGENOMICS SOIL QUALITY AGROECOSYSTEMS

Training Manual for Two-wheel tractor and ancillary equipment for operators, service providers, extension experts and workshop owners

Rabe Yahaya (2022, [Libro])

Mechanization is a term used to describe tools, implements and machinery applied to improve the productivity of farm land and labour force, including crop processing after harvest. Mechanization covers broadly the entire process of on and off farm operations and mechanization may use either human, animal or motorized power, or a combination of these. In practice, therefore, it involves the provision and use of all forms of power sources and mechanical assistance to agriculture, from simple hand tools to draught animal power and to motorized power technologies. In Ethiopia, smallholder farming experiences high drudgery at all stages of crop husbandry and post-harvest processing. Field operations are performed using human and animal power (Mrema et al., 2008). Smallholder farmers without animal power use a lot of human power on crop husbandry and harvesting operations (Daum et al., 2020). Postharvest threshing and shelling are performed using human power but, in some countries like Ethiopia, farmers use livestock (cattle, donkeys and horses) for wheat, barley and teff threshing (Mohammed and Tadesse, 2018). The use of tractor power is low in Ethiopia and the government aims to increase farm power available to Ethiopian farmers 10 folds (from the current 0.1 Kw ha-1 to 1 kW ha-1 ) by 2025, with at least half of this power is derived from fossil fuel and electric engines. The government of Ethiopia aims to increase farm power using two-wheel tractors on smallholder farms in addition to four-wheel tractors that are already in use. Two-wheel tractors are sources of power designed to perform most field operations. Due to the size of two-wheel tractors, they have become an economic alternative for smallholder farming. In addition, twowheel tractors are also more productive than animal traction and they require less time for attendance and preparation, giving the individual farmer more independence and contact with modern technology. Also, due to their simple design, local manufacturing of two-wheel tractors has been implemented in several countries successfully, increasing employment opportunities in the process.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA FARM EQUIPMENT TRACTORS TRAINING SMALLHOLDERS

Review of Nationally Determined Contributions (NCD) of China from the perspective of food systems

Tek Sapkota (2023, [Documento de trabajo])

China is the largest emitter of greenhouse gases (GHG) and one of the countries most affected by climate change. China's food systems are a major contributor to climate change: in 2018, China's food systems emitted 1.09 billion tons of carbondioxide equivalent (CO2eq) GHGs, accounting for 8.2% of total national GHG emissions and 2% of global emissions. According to the Third National Communication (TNC) Report, in 2010, GHG emissions from energy, industrial processes, agriculture, and waste accounted for 78.6%, 12.3%, 7.9%, and 1.2% of total emissions, respectively, (excluding emissions from land use, land-use change and forestry (LULUCF). Total GHG emissions from the waste sector in 2010 were 132 Mt CO2 eq, with municipal solid waste landfills accounting for 56 Mt. The average temperature in China has risen by 1.1°C over the last century (1908–2007), while nationally averaged precipitation amounts have increased significantly over the last 50 years. The sea level and sea surface temperature have risen by 90 mm and 0.9°C respectively in the last 30 years. A regional climate model predicted an annual mean temperature increase of 1.3–2.1°C by 2020 (2.3–3.3°C by 2050), while another model predicted a 1–1.6°C temperature increase and a 3.3–3.7 percent increase in precipitation between 2011 and 2020, depending on the emissions scenario. By 2030, sea level rise along coastal areas could be 0.01–0.16 meters, increasing the likelihood of flooding and intensified storm surges and causing the degradation of wetlands, mangroves, and coral reefs. Addressing climate change is a common human cause, and China places a high value on combating climate change. Climate change has been incorporated into national economic and social development plans, with equal emphasis on mitigation and adaptation to climate change, including an updated Nationally Determined Contribution (NDC) in 2021. The following overarching targets are included in China's updated NDC: • Peaking carbon dioxide emissions “before 2030” and achieving carbon neutrality before 2060. • Lowering carbon intensity by “over 65%” by 2030 from the 2005 level. • Increasing forest stock volume by around 6 billion cubic meters in 2030 from the 2005 level. The targets have come from several commitments made at various events, while China has explained very well the process adopted to produce its third national communication report. An examination of China's NDC reveals that it has failed to establish quantifiable and measurable targets in the agricultural sectors. According to the analysis of the breakdown of food systems and their inclusion in the NDC, the majority of food system activities are poorly mentioned. China's interventions or ambitions in this sector have received very little attention. The adaptation component is mentioned in the NDC, but is not found to be sector-specific or comprehensive. A few studies have rated the Chinese NDC as insufficient, one of the reasons being its failure to list the breakdown of each sector's clear pathway to achieving its goals. China's NDC lacks quantified data on food system sub-sectors. Climate Action Trackers' "Insufficient" rating indicates that China's domestic target for 2030 requires significant improvements to be consistent with the Paris Agreement's target of 1.5°C temperature limit. Some efforts are being made: for example, scientists from the Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences (IEDA-CAAS) have developed methods for calculating GHG emissions from livestock and poultry farmers that have been published as an industrial standard by the Ministry of Agriculture and Rural Affairs, PRC (Prof Hongmin Dong, personal communication) but this still needs to be consolidated and linked to China’s NDC. The updated Nationally Determined Contributions fall short of quantifiable targets in agriculture and food systems as a whole, necessitating clear pathways. China's NDC is found to be heavily focused on a few sectors, including energy, transportation, and urban-rural development. The agricultural sectors' and food systems' targets are vague, and China's agrifood system has a large carbon footprint. As a result, China should focus on managing the food system (production, processing, transportation, and food waste management) to reduce carbon emissions. Furthermore, China should take additional measures to make its climate actions more comprehensive, quantifiable, and measurable, such as setting ambitious and clear targets for the agriculture sector, including activity-specific GHG-reduction pathways; prioritizing food waste and loss reduction and management; promoting sustainable livestock production and low carbon diets; reducing chemical pollution; minimizing the use of fossil fuel in the agri-system and focusing on developing green jobs, technological advancement and promoting climate-smart agriculture; promoting indigenous practices and locally led adaptation; restoring degraded agricultural soils and enhancing cooperation and private partnership. China should also prepare detailed NDC implementation plans including actions and the GHG reduction from conditional targets.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA GREENHOUSE GAS EMISSIONS CLIMATE CHANGE FOOD SYSTEMS LAND USE CHANGE AGRICULTURE POLICIES DATA ANALYSIS FOOD WASTES

Genotyping by sequencing advancements in barley

Naeela Qureshi Mohammad Pourkheirandish (2022, [Artículo])

Genotyping by Sequencing Target Enrichment CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA BARLEY GENOTYPING RESTRICTION ENZYMES SINGLE NUCLEOTIDE POLYMORPHISM

Review of Nationally Determined Contributions (NCD) of Colombia from the perspective of food systems

Tek Sapkota (2023, [Documento de trabajo])

Food is a vital component of Colombia's economy. The impact of climate change on agriculture and food security in the country is severe. The effects have resulted in decreased production and in the productivity of agricultural soil. Desertification processes are accelerating and intensifying. Colombia's government formally submitted its Nationally Determined Contribution (NDC) on December 29, 2020. This paper examines Colombia's NDC from the standpoint of the food system.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CLIMATE CHANGE GREENHOUSE GAS EMISSIONS FOOD SYSTEMS LAND USE CHANGE AGRICULTURE POLICIES DATA ANALYSIS FOOD WASTES

New flavors from old wheats: exploring the aroma profiles and sensory attributes of local Mediterranean wheat landraces

Alon Cna'ani Vered Tzin Maria Itria Ibba Hector Gonzalez-Santoyo (2023, [Artículo])

Wheat Landraces Wholemeal Flour Aroma Compounds Sourdough Bread CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AMINO ACIDS FLAVOUR COMPOUNDS HARD WHEAT ORGANIC ACIDS WHOLE GRAIN FLOUR

Impact of manures and fertilizers on yield and soil properties in a rice-wheat cropping system

Alison Laing Akbar Hossain (2023, [Artículo])

The use of chemical fertilizers under a rice-wheat cropping system (RWCS) has led to the emergence of micronutrient deficiency and decreased crop productivity. Thus, the experiment was conducted with the aim that the use of organic amendments would sustain productivity and improve the soil nutrient status under RWCS. A three-year experiment was conducted with different organic manures i.e. no manure (M0), farmyard manure@15 t ha-1 (M1), poultry manure@6 t ha-1(M2), press mud@15 t ha-1(M3), rice straw compost@6 t ha-1(M4) along with different levels of the recommended dose of fertilizer (RDF) i.e. 0% (F1), 75% (F2 and 100% (F3 in a split-plot design with three replications and plot size of 6 m x 1.2 m. Laboratory-based analysis of different soil as well as plant parameters was done using standard methodologies. The use of manures considerably improved the crop yield, macronutrients viz. nitrogen, phosphorus, potassium and micronutrients such as zinc, iron, manganese and copper, uptake in both the crops because of nutrient release from decomposed organic matter. Additionally, the increase in fertilizer dose increased these parameters. The system productivity was maximum recorded under F3M1 (13,052 kg ha-1) and results were statistically identical with F3M2 and F3M3. The significant upsurge of macro and micro-nutrients in soil and its correlation with yield outcomes was also observed through the combined use of manures as well as fertilizers. This study concluded that the use of 100% RDF integrated with organic manures, particularly farmyard manure would be a beneficial resource for increased crop yield, soil nutrient status and system productivity in RWCS in different regions of India.

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA ORGANIC FERTILIZERS YIELDS SOIL PROPERTIES RICE WHEAT CROPPING SYSTEMS

Evaluación de un reactor biológico empacado con cubos de poliuretano para la remoción de materia orgánica y nutrientes

TANIA GUTIERREZ MACIAS Petia Mijaylova Nacheva (2017, [Conferencia])

El objetivo de este trabajo fue evaluar el uso de cubos de poliuretano como medio de soporte de la biopelícula en un reactor aerobio el cual operó de manera continua y flujo descendente durante un periodo de 90 días. Se aplicó una carga orgánica de 1.41 gDQO·m-3·d-1 con un tiempo de residencia hidráulica (TRH) de 4 h y se dio seguimiento al proceso determinando los siguientes parámetros: Demanda Química de Oxigeno (DQO), solidos suspendidos totales (SST) y solidos suspendidos volátiles (SSV), nitrógeno total (NT), nitrógeno amoniacal (N-NH4) y fósforo total (PT). Como resultado se observó que los microorganismos presentes en el agua residual, colonizaron el medio de soporte (cubos de poliuretano) en los primeros 30 días de operación del sistema, alcanzando una remoción de DQO de 50 %. En el transcurso del tiempo la remoción de DQO aumentó hasta lograr remociones máximas de 84 %. Las remociones obtenidas de SST y SSV fueron mayores de 83 %. La remoción de nutrientes registró valores de 50% NT, 95 % de N- NH4 y 29 % PT.

Reactores biológicos Materia orgánica INGENIERÍA Y TECNOLOGÍA