Bacterial communities in the rhizosphere at different growth stages of maize cultivated in soil under conventional and conservation agricultural practices

Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Farmers in Mexico till soil intensively, remove crop residues for fodder and grow maize often in monoculture. Conservation agriculture (CA), including minimal tillage, crop residue retention and crop diversification, is proposed as a more sustainable alternative. In this study, we determined the effect of agricultural practices and the developing maize rhizosphere on soil bacterial communities. Bulk and maize (Zea mays L.) rhizosphere soil under conventional practices (CP) and CA were sampled during the vegetative, flowering and grain filling stage, and 16S rRNA metabarcoding was used to assess bacterial diversity and community structure. The functional diversity was inferred from the bacterial taxa using PICRUSt. Conservation agriculture positively affected taxonomic and functional diversity compared to CP. The agricultural practice was the most important factor in defining the structure of bacterial communities, even more so than rhizosphere and plant growth stage. The rhizosphere enriched fast growing copiotrophic bacteria, such as Rhizobiales, Sphingomonadales, Xanthomonadales, and Burkholderiales, while in the bulk soil of CP other copiotrophs were enriched, e.g., Halomonas and Bacillus. The bacterial community in the maize bulk soil resembled each other more than in the rhizosphere of CA and CP. The bacterial community structure, and taxonomic and functional diversity in the maize rhizosphere changed with maize development and the differences between the bulk soil and the rhizosphere were more accentuated when the plant aged. Although agricultural practices did not alter the effect of the rhizosphere on the soil bacterial communities in the flowering and grain filling stage, they did in the vegetative stage.

Community Assembly Functional Diversity Intensive Agricultural Practices Plant Microbiome CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA SUSTAINABLE AGRICULTURE TILLAGE SOIL BACTERIA MAIZE

Autotrophic denitrification of nitrate rich wastewater in fluidized bed reactors using pyrite and elemental sulfur as electron donors

Maria Federica Carboni Simon Mills SONIA LORENA ARRIAGA GARCIA Gavin Collins Umer Zeeshan Ijaz Piet Nicolaas Luc Lens (2022, [Artículo])

"This study compared denitrification performances and microbial communities in fluidized bed reactors (FBRs) carrying out autotrophic denitrification using elemental sulfur (S0) and pyrite (FeS2) as electron donors. The reactors were operated for 220 days with nitrate loading rates varying between 23 and 200 mg N-NO-3 /Lmiddotd and HRT between 48 and 4 h. The highest denitrification rates achieved were 142.2 and 184.4 mg NNO-3 /Lmiddotd in pyrite and sulfur FBRs, respectively. Pyrite-driven denitrification produced less SO2- 4 and no buffer addition was needed to regulate the pH. The sulfur FBR needed instead CaCO3 to maintain the pH neutral and consequentially more sludge was produced (CaSO4 precipitation). The active community of pyrite-based systems was investigated and Azospira sp., Ferruginibacter sp., Rhodococcus sp. and Pseudomonas sp. were the predominant genera, while Thiobacillus sp. and Sulfurovum sp. dominated the active community in the sulfur FBR. However, Thiobacillus sp. became more dominant when operating at elevated nitrogen loading rate. Patterns of diversity and microbial community assembly were assessed and revealed three distinct stages of microbial community succession which corresponded with the operation of a period of high influent nitrate concentration (135 mg N-NO-3 /L). It is proposed that a high degree of functional redundancy in the initial microbial communities may have helped both reactors to respond better to such high influent nitrate concentration."

Pyrite Elemental sulfur Fluidized bed rector Nitrogen removal 16S rRNA Community assembly CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Climate-smart agricultural practices influence the fungal communities and soil properties under major agri-food systems

madhu choudhary ML JAT Parbodh Chander Sharma (2022, [Artículo])

Fungal communities in agricultural soils are assumed to be affected by climate, weather, and anthropogenic activities, and magnitude of their effect depends on the agricultural activities. Therefore, a study was conducted to investigate the impact of the portfolio of management practices on fungal communities and soil physical–chemical properties. The study comprised different climate-smart agriculture (CSA)-based management scenarios (Sc) established on the principles of conservation agriculture (CA), namely, ScI is conventional tillage-based rice–wheat rotation, ScII is partial CA-based rice–wheat–mungbean, ScIII is partial CSA-based rice–wheat–mungbean, ScIV is partial CSA-based maize–wheat–mungbean, and ScV and ScVI are CSA-based scenarios and similar to ScIII and ScIV, respectively, except for fertigation method. All the scenarios were flood irrigated except the ScV and ScVI where water and nitrogen were given through subsurface drip irrigation. Soils of these scenarios were collected from 0 to 15 cm depth and analyzed by Illumina paired-end sequencing of Internal Transcribed Spacer regions (ITS1 and ITS2) for the study of fungal community composition. Analysis of 5 million processed sequences showed a higher Shannon diversity index of 1.47 times and a Simpson index of 1.12 times in maize-based CSA scenarios (ScIV and ScVI) compared with rice-based CSA scenarios (ScIII and ScV). Seven phyla were present in all the scenarios, where Ascomycota was the most abundant phyla and it was followed by Basidiomycota and Zygomycota. Ascomycota was found more abundant in rice-based CSA scenarios as compared to maize-based CSA scenarios. Soil organic carbon and nitrogen were found to be 1.62 and 1.25 times higher in CSA scenarios compared with other scenarios. Bulk density was found highest in farmers' practice (Sc1); however, mean weight diameter and water-stable aggregates were found lowest in ScI. Soil physical, chemical, and biological properties were found better under CSA-based practices, which also increased the wheat grain yield by 12.5% and system yield by 18.8%. These results indicate that bundling/layering of smart agricultural practices over farmers' practices has tremendous effects on soil properties, and hence play an important role in sustaining soil quality/health.

Agriculture Management Fungal Community Diversity Indices Climate-Smart Agricultural Practices CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURE TILLAGE CLIMATE-SMART AGRICULTURE SOIL ORGANIC CARBON

Análisis cuantitativo con diagrama de precedencias de condiciones productivas actuales de 5 líneas de ensamble de amortiguadores mediante el método de balanceo peso posicional

José Humberto Vergara García (2023, [Tesis de maestría])

En este trabajo de investigación se empleó el método de Helgeson & Birnie para realizar el balanceo por peso posicional de cinco líneas de ensamble modular de amortiguadores de la empresa ZF Suspension Technology Guadalajara S.A. de C.V. La metodología empleada permitió establecer las condiciones actuales de operación de las cinco líneas. Mediante el uso del método mencionado se encontró que en la mayoría de las líneas de ensamble analizadas sus tareas se encuentran correctamente balanceadas y ordenadas, corroborando así el buen trabajo realizado al momento de su instalación y puesta en marcha. Si bien en cualquier proceso de ensamble siempre hay oportunidades de mejora, contar con líneas de ensamble bien balanceadas permite a la empresa tener una base sólida para la producción de este tipo de componentes automotrices.

The Helgeson & Birnie method was employed in this research for balancing, by positional weight, five modular strut assembly production lines at ZF Suspension Technology Guadalajara S.A. De C.V. The proposed methodology allowed to know the current operating conditions of the production lines. It was found that most of the analyzed production lines are correctly balanced suggesting a correct commissioning and start up procedure performed when the lines were initially installed. Although every assembling line always can be improved, having well-balanced assembly lines provides a solid base line for any automotive manufacturing company.

Líneas de ensamble modular Método de Helgeson & Birnie Peso posicional Final assembly lines Helgeson & Birnie method Positional weight INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

Géneros y afectos en la espacialidad universitaria: perspectivas para el diseño de campus futuros

Griselda Flesler Carolina Spataro RAFAEL BLANCO (2023, [Capítulo de libro])

Este trabajo analiza el impacto en el espacio universitario de las políticas de género implementadas en el ámbito de la Universidad de Buenos Aires (UBA, Argentina) entre los años 2015 y 2020. Se trata de un estudio de caso realizado en el ámbito de la Ciudad Universitaria, campus en el que se emplazan las facultades de Ciencias Exactas y Naturales (FCEyN) y de Arquitectura, Diseño y Urbanismo (FADU). El propósito de este artículo es problematizar la relación entre afecto y espacialidad a partir de los resultados de una encuesta que se propuso identificar cuáles son las emociones que suscitan algunos espacios cotidianos para quienes trabajan y estudian en el campus de la UBA, en particular, aquellos intervenidos con políticas de género durante los últimos años.

This paper analyzes the impact on spaciality of gender policies implemented at the University of Buenos Aires (UBA, Argentina) between 2015 and 2020. It is a case study carried out in the campus where the Faculties of Exact and Natural Sciences (FCEyN) and Architecture, Design and Urbanism (FADU) are located. The purpose of this article is to problematize the relationship between affective perception and spatiality based on the results of a survey that aimed to identify the emotions that some daily spaces arouse for those who work and study on the UBA Campus, in particular, those intervened with gender policies in recent years.

Universidad, espacio, género, giro afectivo. University, space, gender, affective turn. Universidad de Buenos Aires. Gender mainstreaming. Community and college. Incorporación de la perspectiva de género. Universidades. Comunidad y universidad. LC191.98.A74 HUMANIDADES Y CIENCIAS DE LA CONDUCTA PEDAGOGÍA ORGANIZACIÓN Y PLANIFICACIÓN DE LA EDUCACIÓN

Nitrogen fertilizer application alters the root endophyte bacterial microbiome in maize plants, but not in the stem or rhizosphere soil

Alejandra Miranda Carrazco Yendi Navarro-Noya Bram Govaerts Nele Verhulst Luc Dendooven (2022, [Artículo])

Plant-associated microorganisms that affect plant development, their composition, and their functionality are determined by the host, soil conditions, and agricultural practices. How agricultural practices affect the rhizosphere microbiome has been well studied, but less is known about how they might affect plant endophytes. In this study, the metagenomic DNA from the rhizosphere and endophyte communities of root and stem of maize plants was extracted and sequenced with the “diversity arrays technology sequencing,” while the bacterial community and functionality (organized by subsystems from general to specific functions) were investigated in crops cultivated with or without tillage and with or without N fertilizer application. Tillage had a small significant effect on the bacterial community in the rhizosphere, but N fertilizer had a highly significant effect on the roots, but not on the rhizosphere or stem. The relative abundance of many bacterial species was significantly different in the roots and stem of fertilized maize plants, but not in the unfertilized ones. The abundance of N cycle genes was affected by N fertilization application, most accentuated in the roots. How these changes in bacterial composition and N genes composition might affect plant development or crop yields has still to be unraveled.

Bacterial Community Structure DArT-Seq Bacterial Community Functionality Genes Involved in N Cycling CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA AGRICULTURAL PRACTICES MAIZE RHIZOSPHERE STEMS NITROGEN FERTILIZERS

Assessing the Response of Nematode Communities to Climate Change-Driven Warming: A Microcosm Experiment

RUTH GINGOLD WERMUTH (2013, [Artículo])

Biodiversity has diminished over the past decades with climate change being among the main responsible factors. One consequence of climate change is the increase in sea surface temperature, which, together with long exposure periods in intertidal areas, may exceed the tolerance level of benthic organisms. Benthic communities may suffer structural changes due to the loss of species or functional groups, putting ecological services at risk. In sandy beaches, free-living marine nematodes usually are the most abundant and diverse group of intertidal meiofauna, playing an important role in the benthic food web. While apparently many functionally similar nematode species co-exist temporally and spatially, experimental results on selected bacterivore species suggest no functional overlap, but rather an idiosyncratic contribution to ecosystem functioning. However, we hypothesize that functional redundancy is more likely to observe when taking into account the entire diversity of natural assemblages. We conducted a microcosm experiment with two natural communities to assess their stress response to elevated temperature. The two communities differed in diversity (high [HD] vs. low [LD]) and environmental origin (harsh vs. moderate conditions). We assessed their stress resistance to the experimental treatment in terms of species and diversity changes, and their function in terms of abundance, biomass, and trophic diversity. According to the Insurance Hypothesis, we hypothesized that the HD community would cope better with the stressful treatment due to species functional overlap, whereas the LD community functioning would benefit from species better adapted to harsh conditions. Our results indicate no evidence of functional redundancy in the studied nematofaunal communities. The species loss was more prominent and size specific in the HD; large predators and omnivores were lost, which may have important consequences for the benthic food web. Yet, we found evidence for alternative diversity-ecosystem functioning relationships, such as the Rivets and the Idiosyncrasy Model. © 2013 Gingold et al.

aquaculture, article, bacterivore, benthos, biodiversity, biomass, climate, community dynamics, controlled study, ecosystem, environmental temperature, microcosm, nematode, nonhuman, population abundance, species diversity, species richness, taxonomy CIENCIAS FÍSICO MATEMÁTICAS Y CIENCIAS DE LA TIERRA CIENCIAS DE LA TIERRA Y DEL ESPACIO OCEANOGRAFÍA OCEANOGRAFÍA