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Spatio-temporal variation of terpenoids in wild plants of Pentalinon andrieuxii

MICKEL RANDOLPH HIEBERT GIESBRECHT FABIOLA ESCALANTE EROSA KARLINA GARCIA SOSA GABRIEL ROLANDO DZIB LUZ MARIA DEL CARMEN CALVO IRABIEN LUIS MANUEL PEÑA RODRIGUEZ (2016)

Pentalinon andrieuxii (Müll.Arg.) B.F.Hansen & Wunderlin (Apocynaceae) is a vine native to the Yucatan peninsula, where it is widely used in Mayan traditional medicine to treat, among other ailments, the wounds caused by cutaneous leishmaniasis. Among the secondary metabolites isolated from P. andrieuxii are the triterpene betulinic acid and the chemically unusual tri-norsesquiterpene urechitol A; however, to date, there is no existing knowledge about the accumulation dynamics of the ubiquitous betulinic acid or the novel urechitol A in the plant. In this article, we report on the accumulation of both secondary metabolites in wild individuals of P. andrieuxii; our results show that while the content of betulinic acid in plant leaves bears no apparent relation to plantontogeny, the content of urechitol A in root tissue is clearly related to plant development. 

Article

APOCYNACEAE BETULINIC ACID PENTALINON ANDRIEUXII SECONDARY METABOLITE ACCUMULATION SESQUITERPENE TRITERPENE BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA

A germin-like protein gene (CchGLP) of Capsicum chinense Jacq. is induced during incompatible interactions and displays Mn-superoxide dismutase activity

MA. FABIOLA LEON GALVAN AHUITZOLT DE JESUS JOAQUIN RAMOS IRINEO TORRES PACHECO ANA PAULINA BARBA DE LA ROSA LORENZO GUEVARA OLVERA MARIO MARTIN GONZALEZ CHAVIRA ROSALIA VIRGINIA OCAMPO VELAZQUEZ ENRIQUE RICO GARCIA RAMON GERARDO GUEVARA GONZALEZ (2011)

"A germin-like gene (CchGLP) cloned from geminivirus-resistant pepper (Capsicum chinense Jacq. Line BG-3821) was characterized and the enzymatic activity of the expressed protein analyzed. The predicted protein consists of 203 amino acids, similar to other germin-like proteins. A highly conserved cupin domain and typical germin boxes, one of them containing three histidines and one glutamate, are also present in CchGLP. A signal peptide was predicted in the first 18 N-terminal amino acids, as well as one putative N-glycosylation site from residues 44-47. CchGLP was expressed in E. coli and the recombinant protein displayed manganese superoxide dismutase (Mn-SOD) activity. Molecular analysis showed that CchGLP is present in one copy in the C. chinense Jacq. genome and was induced in plants by ethylene (Et) and salicylic acid (SA) but not jasmonic acid (JA) applications in the absence of pathogens. Meanwhile, incompatible interactions with either Pepper golden mosaic virus (PepGMV) or Pepper huasteco yellow vein virus (PHYVV) caused local and systemic CchGLP induction in these geminivirus-resistant plants, but not in a susceptible accession. Compatible interactions with PHYVV, PepGMV and oomycete Phytophthora capsici did not induce CchGLP expression. Thus, these results indicate that CchGLP encodes a Mn-SOD, which is induced in the C. chinense geminivirus-resistant line BG-3821, likely using SA and Et signaling pathways during incompatible interactions with geminiviruses PepGMV and PHYVV"

Article

GLP Geminiviruses Mn-SOD Ethylene Salicylic acid BIOLOGÍA Y QUÍMICA

Mechanical properties of PET composites using multiwalled carbon nanotubes functionalized by inorganic and itaconic acids

ALEJANDRO MAY PAT FRANCIS AVILES CETINA JUAN VALERIO CAUICH RODRIGUEZ (2012)

Multi-walled carbon nanotubes (MWCNTs) were oxidized by two different acid treatments and further functionalized with itaconic acid (IA). The functionalized MWCNTs were used to fabricate Poly(ethylene terephthalate) (PET) composites by melt mixing. The presence of functional groups on the surface of the treated MWCNTs was confirmed by infrared spectroscopy and thermogravimetric analysis. The MWCNTs oxidized with a concentrated mixture of HNO 3 and H 2SO 4 exhibited more oxygen containing functional groups (OH, COOH) but also suffer larger structural degradation than those oxidized by a mild treatment based on diluted HNO 3 followed by H 2O 2. PET composites were fabricated using the oxidized-only and oxidized followed by functionalization with IA MWCNTs. PET composites fabricated with MWCNT oxidized by mild conditions showed improved tensile strength and failure strain, while harsh MWCNT oxidation render them overly brittle.

Article

 ITACONIC ACID MECHANICAL PROPERTIES MWCNTS NANOCOMPOSITES POLYMER COMPOSITES INGENIERÍA Y TECNOLOGÍA

Retraso de la senescencia del fruto de tres variedades de limón mexicano con biorreguladores

LAURA OLIVIA ZEA HERNANDEZ (2015)

Tesis (Maestría en Ciencias, especialista en Fisiología Vegetal).- Colegio de Postgraduados, 2015.

Las pérdidas en postcosecha en frutos de limón mexicano son altas debido a su susceptibilidad a daños mecánicos, rompimiento de glándulas de aceite, pérdidas de peso y de color verde. Con la finalidad de alargar la vida de anaquel y preservar características de calidad, se evaluó el uso de bioreguladores en frutos de tres variedades de limón mexicano. Durante el crecimiento del fruto de las variedades ‘Colimex’, ‘Lise’ y ‘Colimon’, los frutos se asperjaron con ácido giberélico (AG3, 300 ppm), y en postcosecha, fueron recubiertos con cera comercial (14% de sólidos) o tratados con 1-metilciclopropeno (1-MCP, 500 ppm). Los frutos se almacenaron 10 días a temperatura ambiente (22±2°C). Se midieron variables de calidad externa e interna. Se encontró que las variedades ‘Colimex’ y ‘Lise’ presentaron mayor contenido de sólidos solubles totales, menor pérdida de peso, y mayor contenido de clorofila, lo que se reflejó en mayor coloración verde, comparadas con ‘Colimon’. Por otro lado, en general, la aplicación precosecha de AG3 aumentó el contenido de sólidos solubles totales, el contenido de ácido ascórbico, el contenido de clorofila, la intensidad del color verde, y redujo la pérdida de peso, pero ‘Colimón’ no respondió a la aplicación de AG3 para reducir la pérdida de peso. La menor pérdida de peso se atribuyó a mayor peso de cutícula, ceras intracuticulares, y cutina en los frutos tratados con AG3 comparados con los frutos no tratados, lo cual redujo la pérdida de vapor de agua a través de la cutícula. En las tres variedades el índice de color se mantuvo en los tratamientos con aplicaciones de AG3, mientras que en los tratamientos sin AG3, los frutos presentaron un índice de color más alto, que se traduce a una coloración menos verde. La aplicación de AG3 a los frutos en desarrollo de limón mexicano les permite mantener su calidad interna y externa al menos durante 10 días de almacenamiento a temperatura ambiente. _______________ DELAY OF THE SENESCENCE OF THE FRUIT OF THREE VARIETIES OF MEXICAN LIME WITH BIOREGULATORS. ABSTRACT: Postharvest losses in Mexican lime fruits are high due to mechanical damage susceptibility, rupture of oil glands, and loss of weight and color green. With the objective of extending shelf life preserving quality characteristics, the use of bioregulators in fruits of three varieties of Mexican lime was evaluated. During fruit growth, fruits of the varieties 'Colimex', 'Lise' and 'Colimon' were sprayed with gibberellic acid (GA3, 300 ppm). After harvest, fruits were either coated with commercial wax (14% solids) or treated with 1-methylcyclopropene (1-MCP, 500 ppm). Fruits were stored 10 days at room temperature (22± 2 °C). Parameters to determine external and internal quality were measured. 'Colimex' and 'Lise' varieties presented a greater total soluble solids content, lower fruit weight loss, and greater chlorophyll content, resulting in greener fruits, compared to 'Colimon'. On the other hand, in general, pre-harvest application of GA3 increased total soluble solids content, ascorbic acid content, chlorophyll content, and intensity of color green, and reduced fruit weight loss. However, 'Colimon' did not reduce fruit weight loss as a response to GA3 application. The smaller fruit weight loss in GA3-treated fruits was attributed to greater weight of cuticle, intracuticular waxes and cutin, compared to non-treated fruits, which reduced water vapor loss through the cuticle. In the three Mexican lime varieties, fruit color index was maintained in all treatments that included GA3 application, while in the treatments without GA3, fruits presented a higher color index, which indicates less green-colored fruits. Preharvest GA3 application to developing fruits of Mexican lime allows the fruit to maintain their internal and external quality at least during 10 days of storage at ambient temperature.

Master thesis

Ácido giberélico 1-MCP Calidad postcosecha Fisiología postcosecha Gibberellic acid Postharvest quality Postharvest physiology Fisiología Vegetal Maestría CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Fenoles de interés farmacológico en hojas de vides silvestres (Vitis spp.) de México

J. Refugio Tobar Reyes OMAR FRANCO MORA Edgar Jesús Morales Rosales JUAN GUILLERMO CRUZ CASTILLO (2011)

Sedentary styles of life, along with inadequate nutritional habits, increase the risk to develop degenerative chronic diseases. Searching for possible alternatives of prevention for those risks, it has been investigated about the quality and nutraceutic properties of several agricultural commodities. The phenols are substances with important functions in vegetal organisms; and moreover they have antioxidants properties in the cellular metabolism of humans and animals. In order to quantify the content of three phenolic acids and a stilbene, it was performed analyses, by high performance liquid chromatography (HPLC-UV/Vis), on metanolic leave extracts of 97 wild grapevines (Vitis spp.) of Puebla, Mexico. Those plants were found growing in their natural environments from 207 to 2175 m. The gallic acid was the most common of the metabolites since it appeared in 96 of the 97 samples, rutin was the second most common substance and it was identified in 80 samples, the caffeic acid appeared in 29 and finally resveratrol only in 14. The leaves of wild grapevines contain diverse antioxidants substances that might have several benefits in the human health; in addition the suitable conservation, maintenance and correct advantage of this plant genus provide environmental services and the balance of ecosystems.

Article

Agrociencias Caffeic acid gallic acid resveratrol rutin CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Timbe (Acaciella angustissima) pods extracts reduce the levels of glucose, insulin and improved physiological parameters, hypolipidemic effect, oxidative stress and renal damage in streptozotocin-induced diabetic rats

ADRIANA JHENY RODRIGUEZ MENDEZ WENDY MONSERRAT CARMEN SANDOVAL MARIA DEL CONSUELO LOMAS SORIA Ramón Gerardo Guevara González ROSALIA REYNOSO CAMACHO MA. ELENA VILLAGRAN HERRERA Luis Antonio Salazar Olivo Irineo Torres Pacheco ANA ANGELICA FEREGRINO PEREZ (2018)

"In Mexico one in 14 deaths are caused by diabetes mellitus (DM) or by the macro and microvascular disorders derived from it. A continuous hyperglycemic state is characteristic of DM, resulting from a sustained state of insulin resistance and/or a dysfunction of ?-pancreatic cells. Acaciella angustissima is a little studied species showing a significant antioxidant activity that can be used as treatment of this disease or preventive against the complications. The objective of this study was to explore the effect of oral administration of A. angustissima methanol extract on physiological parameters of streptozotocin-induced diabetic rats. The results indicated a significant reduction in blood glucose levels, an increase in serum insulin concentration, a decrease in lipid levels and an improvement in the parameters of kidney damage by applying a concentration of 100 mg/Kg B.W. However, glucose uptake activity was not observed in the adipocyte assay. Moreover, the extract of A. angustissima displayed potential for the complementary treatment of diabetes and its complications likely due to the presence of bioactive compounds such as protocatechuic acid. This study demonstrated that methanol extract of Acacciella angustissima has an antidiabetic effect by reducing the levels of glucose, insulin and improved physiological parameters, hypolipidemic effect, oxidative stress and renal damage in diabetic rats."

Article

Acacciella angustissima Streptozotocin-diabetic rats Antidiabetic effect Polyphenolic Protocatechuic acid BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA MOLECULAR BIOLOGÍA MOLECULAR

In vitro inhibition of Botrytis cinerea with extracts of wild grape (Vitis spp.) leaves

ISELA APOLONIO RODRIGUEZ OMAR FRANCO MORA MARTHA LIDYA SALGADO SICLAN JESUS GAUDENCIO AQUINO MARTINEZ (2017)

The extracts of wild grapevines

(Vitis) leaves (EHVS) from three accessions (P178,

E-200 and TN-4) at 6, 8 and 12 % v/v, were

tested in vitro to evaluate their inhibitory effect on

Botrytis cinerea development. Resveratrol (RVS)

(60, 90, 120 µg/mL), citrus extracts (EC) (3, 5 and

8 % v/v) and cyprodinil + fludioxonil (SW) (500,

800 and 1000 µg/mL) were compared against

EHVS. In average, SW presented inhibition of

mycelial growth (ICM), sporulation (IE) and spore

germination (IGC) (88.9; 85.5 and 93.7 %, average

respectively). RVS presented the second higher

inhibition rate. All EHVS presented antifungal

activity; specially, P-178 at 12 % resulted in 72 %

ICM, 75 % IE and 62 % IGC. This dose contained

phenolic compounds 19.9 mg/mL, RVS 1.7 mg/mL,

gallic acid 3.8 mg/mL and ferulic acid 2.5 mg/mL.

CONACYT

Article

Ferulic acid gallic acid antifungal activity phenolic compounds resveratrol CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA

Caracterización fisicoquímica y sensorial del café del estado de Chiapas.

MARIELA CORTÉS FLORES (2020)

Tesis (Maestría en Ciencias, especialista en Innovación Agroalimentaria Sustentable).- Colegio de Postgraduados, Campus Córdoba, 2020.

Chiapas es el principal estado productor de café en México, aportando alrededor del 40% de la producción nacional en 2018 (SIAP, 2019). El estado cuenta con 13 regiones productoras con diversas características ambientales, técnicas y socioculturales que influyen en la producción del grano, por ello, el objetivo de este trabajo fue estudiar las propiedades físico-químicas del grano y evaluar su efecto en los atributos sensoriales de la bebida. Se obtuvieron 139 muestras de 12 kilogramos de café cereza, procesadas por la vía húmeda, al café pergamino resultante se le realizó: análisis físico-químico y sensorial de acuerdo con los protocolos de la SCA para cada muestra. Los resultados mostraron que la forma del grano no afectó el Puntaje Total en taza, pero sí en atributos como el aroma y cuerpo. Mientras que el tamaño de los granos no tuvo un efecto significativo en casi todos los atributos sensoriales de la bebida; solo en aroma, los granos de café medianos y pequeños obtuvieron puntajes más altos que los granos más grandes. La concentración de cafeína y trigonelina en el grano tuvo una correlación negativa con todos los atributos de calidad de la taza, contrario a lo encontrado para el ácido clorogénico (5-CQA), que mejoró todos los atributos, especialmente la acidez. Además, se encontró que 11 de las 13 regiones presentan un puntaje total promedio en taza de 80 puntos o más, destacando Bochil, Pichucalco y Motozintla. En conclusión, las propiedades fisicoquímicas y del grano pueden modular los atributos de calidad de la taza de la bebida en muestras de café de Chiapas, México. _______________ CARACTERIZACIÓN FISICOQUÍMICA Y SENSORIAL DEL CAFÉ DEL ESTADO DE CHIAPAS. ABSTRACT: Chiapas is the main coffee producing state in Mexico, contributing around 40% of national production in 2018 (SIAP, 2019). The state has 13 producing regions with various environmental, technical and socio-cultural characteristics that influence the production of the grain, therefore, the objective of this work was to study the physical-chemical properties of the grain and evaluate its effect on the sensory attributes of the drink. 139 samples of 12 kilograms of cherry coffee, processed by the wet route, were obtained from the resulting parchment coffee: physical-chemical and sensory analysis was performed according to the SCA protocols for each sample. The results showed that the shape of the grain did not affect the Total Cup Score, but in attributes such as aroma and body. While the size of the beans did not have a significant effect on almost all the sensory attributes of the beverage, only in aroma, medium and small coffee beans scored higher than larger beans. The concentration of caffeine and trigonelline in the grain had a negative correlation with all cup quality attributes, contrary to that found for chlorogenic acid (5-CQA), which improved all attributes, especially acidity. In addition, 11 of the 13 regions were found to have an average total cup score of 80 points or more, highlighting Bochil, Pichucalco and Motozintla. In conclusion, the physicochemical and grain properties can modulate the quality attributes of the beverage cup in coffee samples from Chiapas, Mexico.

Master thesis

Cafeína Ácido Clorogénico Altura Café de especialidad Análisis sensorial Caffeine Chlorogenic Acid Height Specialty coffee Sensory analysis Innovación Agroalimentaria y Sustentable Maestría CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA CIENCIAS AGRARIAS AGRONOMÍA SEMILLAS

Arabinoxilanos y la Relación de la Fracción Proteica Remanente con la Capacidad Gelificante del Polisacárido

Arabinoxylans and the remaining protein fraction relationship with the gelling capability of the polysaccharide

MAYRA ALEJANDRA MENDEZ ENCINAS Elizabeth Carvajal Millan Agustín Rascón Chu Yolanda Leticia López Franco Jaime Lizardi Mendoza (2019)

Los arabinoxilanos (AX) son polisacáridos no amiláceos presentes en varios tejidos de cereales. Estos polisacáridos tienen la capacidad de formar geles covalentes mediante el acoplamiento oxidativo del ácido ferúlico por acción de agentes generadores de radicales libres. La capacidad gelificante de los AX depende de las características estructurales del polisacárido, por lo que el conocimiento de estas y su efecto en las propiedades del gel es esencial para crear una comprensión profunda sobre la naturaleza de estos geles. El contenido de proteína en AX es una de estas características estructurales, cuyo impacto en la gelificación del polisacárido y en las propiedades del gel no ha sido investigado a profundidad. Hasta el momento, no se conoce cómo es que la proteína se encuentra unida al AX. Esta revisión presenta investigaciones realizadas sobre la asociación arabinoxilano-proteína y la relación de la fracción proteica con la capacidad gelificante del polisacárido, así como de la posible unión covalente en dicha asociación.

Arabinoxylans (AX) are non-starch polysaccharides present in several tissues of cereal grains. These polysaccharides can form covalent gels through oxidative coupling of ferulic acid by the action of free radical-

generating agents. Since the gelling capability of AX depends on their structural characteristics, the knowledge of structural characteristics a

nd their effect on gel properties is essential for building a thorough understanding about the nature of these gels. Protein content of AX is one of such structural characteristics, whose impact on the gelling of the polysaccharide and gel properties has not been investigated in detail. Till date, it is not known how the protein is attached to the AX. This review presents research done on the arabinoxylan-protein association and the relationship of the protein fraction with the gelling capability of the polysaccharide, as well as the possible covalent binding on that association.

Article

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA Arabinoxilano Enlace covalente Ácido Ferúlico Gelificación Proteína Arabinoxylan Covalent linkage Ferulic acid Gelling Protein

Anti-inflammatory drugs and uterine cervical cancer cells: antineoplastic effect of meclofenamic acid

ALEJANDRO DAVID SORIANO HERNANDEZ DANIELA MADRIGAL PÉREZ HECTOR RAFAEL GALVAN SALAZAR MARGARITA DE LA LUZ MARTINEZ FIERRO LAURA LETICIA VALDEZ VELAZQUEZ FRANCISCO ESPINOZA GOMEZ OSCAR FERNANDO VAZQUEZ VUELVAS BERTHA ALICIA OLMEDO BUENROSTRO JOSE GUZMAN ESQUIVEL IRAM PABLO RODRIGUEZ SANCHEZ AGUSTIN LARA ESQUEDA DANIEL ALBERTO MONTES GALINDO IVAN DELGADO ENCISO (2015)

Uterine cervical cancer (UCC) is one of the main causes of cancer-associated mortality in women. Inflammation has been identified as an important component of this neoplasia; in this context, anti-inflammatory drugs represent possible prophylactic and/or therapeutic alternatives that require further investigation. Anti-inflammatory drugs are common and each one may exhibit a different antineoplastic effect. As a result, the present study investigated different anti-inflammatory models of UCC in vitro and in vivo. Celecoxib, sulindac, nimesulide, dexamethasone, meclofenamic acid, flufenamic acid and mefenamic acid were tested in UCC HeLa, VIPA, INBL and SiHa cell lines.

Article

MEDICINA Y CIENCIAS DE LA SALUD Non-steroidal anti-inflamatory drugs Meclofenamic Acid Antitumor activity Uterine cervical cancer Murine model