Gene expression in primary hemocyte culture of the Pacific white shrimp Penaeus vannamei infected with different white spot syndrome virus (WSSV) strains

Delia Patricia Parrilla Taylor REGINA ELIZONDO GONZALEZ Jesús Neftalí Gutiérrez Rivera SILVIA ALEJANDRA GARCIA GASCA NORBERTO VIBANCO PEREZ MA. DE JESUS DURAN AVELAR RICARDO VAZQUEZ JUAREZ (2022, [Artículo])

"Five previously analyzed white spot syndrome virus (WSSV) strains from northwest Mexico,differing in their genome architecture as well as in virulence, were selected (high virulence JP and LG strains;moderate virulence GVE and DIV strains; and low virulence LC10 strain) to evaluate pathogenesis response in vitro. Expression of phagocytosis-activating protein PAP, manganese superoxide dismutase MnSOD and peroxiredoxin PRX, and two genes of immediate-early expression (IE1 and WSSV304) were measured by qPCR in a primary hemocyte cell culture from Penaeus vannamei at 1, 3, 6, 12, and 24 h post-infection (hpi). PAP expression was significantly higher at 1 and 3 hpi, and JP and LC10 strains induced the highest expression. The response of MnSOD was high at 1 hpi, and a significant increase in PRX expression was detected at 3 hpi, probably due to the occurrence of an oxidative burst; expression levels of MnSOD and PRX were significantly higher at 1 and 3 hpi, respectively, induced by the LG strain (high virulence), suggesting an acute response. In general, expression of most immune-related - genes decreased after the initial hours of infection. Expression levels of IE1 and WSSV304 were exceptionally high at 1 hpi in almost all five WSSV analyzed strains,confirming their efficient mechanism for replication and viral fitness. The results of this study do not show an accurate link between the genome size and WSSV virulence of the strains, albeit the strain with the smallest genome showed the highest virulence. All strains induced an early immune response in heterogeneous ways."

Penaeus vannamei, virulence, gene expression, viral fitness, viral pathogenesis, immune response BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA BIOLOGÍA ANIMAL (ZOOLOGÍA) PATOLOGÍA ANIMAL PATOLOGÍA ANIMAL

Novel Low-cost Energy Harvester Based on anArrangement of Piezoelectric Actuators

CARLOS ANDRES FERRARA BELLO Margarita Tecpoyotl Torres (2024, [Artículo])

This article provides the design, modeling, fabrication, and testing of an energy

harvester from mechanical vibrations, based on a piezoelectric material. This device

works on the principle of piezoelectric transduction, i.e., when mechanically

deformed, it generates electrical energy due to vibrations. The piezoelectric material

used in the fabrication was Lead Zirconate Titanate (PZT), and brass as structural

base. In addition, finite element models were performed to predict the frequency of

the first vibration mode of the device, and experimental setups for validation. The

resonance frequency of the numerical model and the one obtained experimentally (19

Hz) show a deviation of 5.03% respectively. The generated power is 0.202 mW enough

to power low power devices such as basic calculators, wristwatches, and transistors, among others.

En este artículo se presenta el diseño, modelado, fabricación y pruebas de un cosechador de energía proveniente de vibraciones mecánicas, basado en un material piezoeléctrico. Este dispositivo trabaja bajo el principio de transducción piezoeléctrica, es decir que, al deformarse mecánicamente, debido a las vibraciones genera energía eléctrica. El material piezoeléctrico usado en la fabricación fue Zirconato Titanato de Plomo (PZT), y latón como base estructural. Además, se realizaron modelos de elemento finito para predecir la frecuencia del primer modo de vibración del dispositivo, y arreglos experimentales para su validación. La frecuencia de resonancia del modelo numérico y la obtenida experimentalmente (19 Hz) muestran una desviación de 5.03% respectivamente. La potencia generada es de0.202 mW suficiente para alimentar dispositivos de bajo consumo, tales como calculadoras básicas, relojes de pulsera y transistores, entre otros.

INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS Finite Element Method, low-energy devices, vibration, piezoelectric transducer