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3 resultados, página 1 de 1

Transcriptome mining provides insights into cell wall metabolism and fiber lignification in Agave tequilana Weber

Luis Fernando Maceda Lopez ELSA BEATRIZ GONGORA CASTILLO Enrique Ibarra-Laclette DALIA C. MORAN VELAZQUEZ AMARANTA GIRON RAMIREZ Matthieu Bourdon José Luis Villalpando Aguilar Gabriela Chavez-Calvillo Toomer John Tang Parastoo Azadi Jorge Manuel Santamaría Fernández Itzel López-Rosas Mercedes G Lopez June Simpson FULGENCIO ALATORRE COBOS (2022, [Artículo])

Resilience of growing in arid and semiarid regions and a high capacity of accumulating sugar-rich biomass with low lignin percentages have placed Agave species as an emerging bioen-ergy crop. Although transcriptome sequencing of fiber-producing agave species has been explored, molecular bases that control wall cell biogenesis and metabolism in agave species are still poorly understood. Here, through RNAseq data mining, we reconstructed the cellulose biosynthesis pathway and the phenylpropanoid route producing lignin monomers in A. tequilana, and evaluated their expression patterns in silico and experimentally. Most of the orthologs retrieved showed differential expression levels when they were analyzed in different tissues with contrasting cellulose and lignin accumulation. Phylogenetic and structural motif analyses of putative CESA and CAD proteins allowed to identify those potentially involved with secondary cell wall formation. RT-qPCR assays revealed enhanced expression levels of AtqCAD5 and AtqCESA7 in parenchyma cells associated with extraxylary fibers, suggesting a mechanism of formation of sclerenchyma fibers in Agave similar to that reported for xylem cells in model eudicots. Overall, our results provide a framework for un-derstanding molecular bases underlying cell wall biogenesis in Agave species studying mechanisms involving in leaf fiber development in monocots. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.

AGAVE CELL WALLS LIGNOCELLULOSE CAD PROTEIN CESA PROTEIN SCLERENCHYMA BIOLOGÍA Y QUÍMICA CIENCIAS DE LA VIDA GENÉTICA GENÉTICA MOLECULAR DE PLANTAS GENÉTICA MOLECULAR DE PLANTAS

Optimization of the alkali-silane treatment of agave lechuguilla fibers (ixtle) for potential reinforcement in polymeric composites

NOEMI JARDON MAXIMINO MARIAMNE DEHONOR GOMEZ Rolando Villa Moreno MARIA DOLORES BAEZA ALVARADO Luis Edmundo Lugo Uribe (2023, [Artículo])

Reinforced polymeric composites with natural fibers have garnered significant interest in recent years due to the need for biomass utilization and the requirements of various industries, such as automotive and construction. Among these natural fibers, Agave lechuguilla fiber, commonly known as ixtle (FIx) or Tampico fiber, exhibits important characteristics such as length, high strength, and durability. However, there is limited literature on its conditioning, functionalization, and utilization as a reinforcing material in polymeric composites (CP). This study presents the optimization of the alkali-silane treatment of FIx, identifying the most suitable reaction conditions to enhance their thermal stability, tensile strength, and silane coupling agent (ACSi) grafting on the fiber surface. The chemical treatment with ACSi proved highly effective, resulting in a significant grafting content, which was confirmed through FTIR and SEM–EDS analyses. The high level of functionalization did not compromise the mechanical performance of the fibers, suggesting that functionalized FIx holds great potential as a reinforcing material in CP. These findings open new paths for the sustainable use of Agave lechuguilla fibers, contributing to the development of environmentally friendly and high-performance polymeric composites in various industrial applications.

This article belongs to the Special Issue Natural Fibers for Advanced Materials: Addressing Challenges).

Supplementary materials: The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/fib11100086/s1, Figure S1: Optical microscope image of ixtle fibers at a magnification of 50 (a) untreated and (b) alkaline treated (FIx-5); Table S1: FTIR signal assignment for alkaline treated FIx; Table S2: FTIR Signal assignment for silane treated FIx.

Author contributions: Conceptualization, N.J.-M. and L.E.L.U.; methodology, N.J.-M., M.D.G., R.V.M., M.D.B.-A. and L.E.L.U.; validation, N.J.-M. and L.E.L.U.; formal analysis, N.J.-M., M.D.G. and L.E.L.U.; investigation, N.J.-M. and L.E.L.U.; data curation, N.J.-M., M.D.G., R.V.M. and M.D.B.-A.; writing—original draft preparation, N.J.-M. and L.E.L.U.; writing—review and editing, N.J.-M., M.D.G., R.V.M., M.D.B.-A. and L.E.L.U.; visualization, N.J.-M., M.D.G. and L.E.L.U.; supervision, L.E.L.U.; project administration, N.J.-M. and L.E.L.U.; funding acquisition, N.J.-M. and L.E.L.U. All authors have read and agreed to the published version of the manuscript.

Funding: This research was funded by CONAHCYT, “Estancias postdoctorales por México”, grant umber CVU425480.

Data availability statement: Data supporting the findings of this study are available within the article and upon request from the corresponding author.

Acknowledgments: The authors express their gratitude towards Rene Diaz Rebollar and Jazmin Gomez Sara for their technical assistance in conducting the chemical reactions presented in this current study. Additionally, the assistance provided by Luis Alberto Caceres Diaz in the execution of XRD analyses is also acknowledged. Furthermore, N.J.M. extends sincere recognition to CIATEQ A.C. for their provision of essential resources and infrastructure crucial to the advancement of this research. The support rendered by CONAHCYT through the “Estancias postdoctorales por México” program is also gratefully acknowledged, as it has contributed financial backing to the project. Conflicts of Interest: The authors declare no conflict of interest.

Agave lechuguilla Natural fiber Silane coupling agent INGENIERÍA Y TECNOLOGÍA CIENCIAS TECNOLÓGICAS OTRAS ESPECIALIDADES TECNOLÓGICAS OTRAS OTRAS

Análisis del pretratamiento de residuos lignocelulósicos para la producción de biocombustibles y bioproductos de alto valor agregado.

Perla Araceli Meléndez Hernández JAVIER ULISES HERNANDEZ BELTRAN HECTOR HERNANDEZ ESCOTO RICARDO MORALES RODRIGUEZ (2015, [Artículo])

La búsqueda de nuevas formas de energía renovables ha impulsado el estudio de los biocombustibles a partir de la biomasa lignocelulósica como lo son la paja de trigo, de sorgo o bagazo de caña de azúcar, de la cual se obtienen los azúcares reductores fermentables mediante una hidrólisis enzimática. Para poder utilizar los azúcares reductores que posee esta biomasa, es necesario dar un pretratamiento previo. Por tanto, en este trabajo se hace un análisis de dos tipos de pretratamiento para dos tipos de bagazo de caña (bagazo natural y bagazo hidrolizado): el primer pretratamiento consiste en una autohidrólisis a 121ºC y el segundo en un pretratamiento alcalino-oxidativo. Los productos de los dos tipos de pretratamiento se utilizaron para realizar una hidrólisis enzimática utilizando el complejo comercial Accellerase 1500, donde se encontró que el mejor pretratamiento fue el alcalino-oxidativo donde se obtuvieron hasta 12 g/L de glucosa para el bagazo hidrolizado (78.68% g/g) y 9.9 g/L para el bagazo natural (60.20% g/g), mientras que en la hidrólisis enzimática del bagazo pretratado mediante la autohidrólisis se obtuvieron concentraciones de 0.704 g/L para el bagazo natural (6.67% g/g) y de 1.899g/L para el bagazo hidrolizado (19.65% g/g).

The search for new forms of renewable energy has promoted the study of biofuels from lignocellulosic biomass such as wheat straw, sorghum straw and sugarcane bagasse, which are the source to obtain reducing fermentable sugar through an enzymatic hydrolysis. To make use of these reducing sugars is necessary to perform a pretreatment. This research analyses two kinds of pretreatment for two types of bagasse (natural bagasse and hydrolysed bagasse): the first pretreatment consists of an autohydrolysis at 121ºC and the second pretreatment is an alkaline-oxidative pretreatment. The products of these two types of pretreatments were used for enzymatic hydrolysis using the commercial complex Accellerase 1500, where it was found that the best pretreatment was the alkaline-oxidative which was obtained 12g/L of glucose to the hydrolyzed bagasse (78.68% g/g) and 9.9 g/L for natural bagasse (60.20% g/g) while in the enzymatic autohydrolysis concentration of 0.704 g/L for natural bagasse (6.67% g/g) were obtained and 1,899 g/L for the hydrolyzed bagasse (19.65% g/g).

CIENCIAS AGROPECUARIAS Y BIOTECNOLOGÍA Bagazo de caña de azúcar Autohidrólisis Hidrolisis enzimática Pretratamiento alcalino