Título
EGF-Coupled gold nanoparticles increase the expression of CNPase and the Myelin-Associated Proteins MAG, MOG, and MBP in the septal nucleus demyelinated by cuprizone
Autor
EDUARDO LIRA DIAZ
MARIA GUADALUPE GONZALEZ PEDROZA
LUIS ALBERTO CASTRO SANCHEZ
OSCAR PEREZ GONZALEZ
Nivel de Acceso
Acceso Abierto
Identificador alterno
isni/0000 0004 0428 7635
Materias
Resumen o descripción
Current pharmacological therapies against demyelinating diseases are not quite satis-factory to promote remyelination. Epidermal growth factor (EGF) can expand the population of oligodendrocyte precursor cells (OPCs) that may help with the remyelination process, but its delivery into the injured tissue is still a biomedical challenge. Gold nanoparticles (GNPs) may be a useful tool for drug delivery into the brain. To evaluate remyelination in the septal nucleus, we administered intracerebral GNPs coupled with EGF(EGF–GNPs). C57BL6/J mice were demyelinated with 0.4% cuprizone (CPZ) and divided into severalgroups: Sham, Ctrl, GNPs, EGF, and EGF–GNPs. We evaluated the remyelination process at twotime-points: 2 weeks and 3 weeks post-injection (WPI) of each treatment. We used the rotarod forevaluating motor coordination. Then, we did a Western blot analysis myelin-associated proteins:CNPase, MAG, MOG, and MBP. EGF–GNPs increase the expression of CNPase, MAG, and MOGat 2 WPI. At 3 WPI, we found that the EGF–GNPs treatment improves motor coordination andincreases MAG, MOG, and MBP. EGF–GNPs enhance the expression of myelin-associated proteins andimprove the motor coordination in mice. Thus, EGF-associated GNPs may be a promisingpharmacological vehicle for delivering long-lasting drugs into the brain. © 2022 by the authors. LicenseeMDPI, Basel, Switzerland.
Editor
MDPI
Fecha de publicación
23 de febrero de 2022
Tipo de publicación
Artículo
Recurso de información
Idioma
Inglés
Repositorio Orígen
Repositorio institucional de la Universidad de Colima
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