Título

miRNAs contained in extracellular vesicles cargo contribute to the progression of idiopathic pulmonary fibrosis: an in vitro aproach

Autor

JOVITO CESAR SANTOS ALVAREZ

JUAN MANUEL VELAZQUEZ ENRIQUEZ

ROSENDO GARCÍA CARRILLO

CESAR RODRIGUEZ BEAS

ALMA AURORA RAMIREZ HERNANDEZ

EDILBURGA REYES JIMENEZ

KARINA GONZÁLEZ GARCÍA

ARMANDO LOPEZ MARTINEZ

LAURA PEREZ CAMPOS MAYORAL

SERGIO ROBERTO AGUILAR RUIZ

MARIA DE LOS ANGELES ROMERO TLALOLINI

HONORIO TORRES AGUILAR

LUIS ALBERTO CASTRO SANCHEZ

JAIME ARELLANES ROBLEDO

VERONICA ROCIO VASQUEZ GARZON

RAFAEL BALTIERREZ HOYOS

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by/4.0

Identificador alterno

isni/0000 0004 0428 7635

isni/0000 0001 1305 1454

Materias

MEDICINA Y CIENCIAS DE LA SALUD - (CTI) Idiopathic pulmonary fibrosis - (SIN FUENTE) Extracellular vesicles - (SIN FUENTE) Fibroblasts - (SIN FUENTE) Small-RNA seq - (SIN FUENTE)

Resumen o descripción

Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive lung disease. Lesions in the lung epithelium cause alterations in the microenvironment that promote fibroblast accumulation. Extracellular vesicles (EVs) transport proteins, lipids, and nucleic acids, such as microRNAs (miRNAs). The aim of this study was to characterize the differentially expressed miRNAs in the cargo of EVs obtained from the LL97 and LL29 fibroblast cell lines isolated from IPF lungs versus those derived from the CCD19 fibroblast cell line isolated from a healthy donors. We characterized EVs by ultracentrifugation, Western blotting, and dynamic light scattering. We identified miRNAs by small RNA-seq, a total of 1144 miRNAs, of which

1027 were known miRNAs; interestingly, 117 miRNAs were novel. Differential expression analysis showed that 77 miRNAs were upregulated and 68 were downregulated. In addition, pathway enrichment analyses from the Gene Ontology and Kyoto Encyclopedia of Genomes identified several miRNA target genes in the categories, cell proliferation, regulation of apoptosis, pathways in cancer, and proteoglycans in cancer. Our data reveal that miRNAs contained in EVs cargo could be helpful as biomarkers for fibrogenesis, diagnosis, and therapeutic intervention of IPF.

Editor

MDPI

Fecha de publicación

25 de marzo de 2022

Tipo de publicación

Artículo

Recurso de información

http://biblos.ucol.mx//handle/123456789/117

Idioma

Inglés

Repositorio Orígen

Repositorio institucional de la Universidad de Colima

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