Title
Chronic toxicity of shrimp feed added with silver nanoparticles (Argovit-4®) in Litopenaeus vannamei and immune response to white spot syndrome virus infection
Author
Carlos Rosendo Romo Quiñonez
Píndaro Álvarez Ruiz
CLAUDIO HUMBERTO MEJIA RUIZ
NINA BOGDANCHIKOVA
Alexey Pestryakov
CARINA GAMEZ JIMENEZ
WENCESLAO VALENZUELA QUIÑONEZ
Magnolia Montoya Mejía
EUSEBIO NAVA PEREZ
Access level
Open Access
Publication reference
doi: DOI: 10.7717/peerj.14231
ISSN/ISSN: 21678359
URL/URL: https://peerj.com/articles/14231/
Subjects
Summary or description
"In recent years, the application of silver nanoparticles (AgNPs) as antibacterial compounds has been widely used in human and veterinary medicine. In this work, we investigated the effects of AgNPs (Argovit-4 R ) as feed additives (feed-AgNPs) on shrimp (Litopenaeus vannamei) using three different methods: 1) chronic toxicity after 28 days of feeding, 2) Effects against white spot syndrome virus (WSSV) challenged by oral route, and 3) transcriptional responses of immune-related genes (PAP, ProPO, CTL-3, Crustin, PEN3, and PEN4) following WSSV infection. The results showed that the feed-AgNPs did not interfere with the growth and survival of shrimp. Also, mild lesions in the hepatopancreas were recorded, proportional to the frequency of the feed-AgNP supply. Challenge test versus WSSV showed that feeding every 7 days with feed-AgNPs reduced mortality, reaching a survival rate of 53%, compared to the survival rates observed in groups fed every 4 days, daily and control groups of feed-AgNPs for the 30%, 10%, and 7% groups, respectively. Feed-AgNPs negatively regulated the expression of PAP, ProPO, and Crustin genes after 28 days of treatment and altered the transcriptional responses of PAP, ProPO, CTL-3, and Crustin after WSSV exposure. The results showed that weekly feeding-AgNPs could partially prevent WSSV infection in shrimp culture. However, whether or not transcriptional responses against pathogens are advantageous remains to be elucidated."
Publisher
PeerJ Inc.
Publish date
2022
Publication type
Article
Publication version
Published Version
Information Resource
Format
application/pdf
Source
PeerJ
Language
English
Citation suggestion
Romo Quiñonez CR, Alvarez-Ruiz P, Mejía-Ruiz CH, Bogdanchikova N, Pestryakov A, Gamez-Jimenez C, Valenzuela-Quiñonez W, Montoya-Mejía M, Nava Pérez E. 2022. Chronic toxicity of shrimp feed added with silver nanoparticles (Argovit-4®) in Litopenaeus vannamei and immune response to white spot syndrome virus infection. PeerJ 10:e14231 https://doi.org/10.7717/peerj.14231
Source repository
Repositorio Institucional CIBNOR
Downloads
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