Título
In vitro Digestibility of Yarrowia lipolytica Yeast and Growth Performance in Whiteleg Shrimp Litopenaeus vannamei
Autor
ANA RUTH ALVAREZ SANCHEZ
CLAUDIO HUMBERTO MEJIA RUIZ
Héctor Gerardo Nolasco Soria
Alberto Peña Rodríguez
Nivel de Acceso
Acceso Abierto
Referencia de publicación
doi: DOI: 10.4194/1303-2712-v18_3_05
URL/URL: http://www.trjfas.org
ISSN/ISSN: 1303-2712
Materias
Resumen o descripción
"Marine yeasts used in aquaculture disease control can also be an important protein source for improving feeding and nutrition of crustaceans. Yarrowia lipolyticca has been studied for its capacity to secrete heterologous proteins and high content of unsaturated fatty acids, beta-glucan, and mannane polymers in the cell wall. We measured in vitro digestibility of Y. lipolyticca by whiteleg shrimp Litopenaeus vannamei digestive enzymes, and an in vivo assay of Y. lipolytica in feed onwhiteleg shrimp growth. We found that digestive gland enzymes of shrimp digest Y. lipolytica, based on reduced optical density of a yeast suspension. Digestion was –0.00236 ± 0.00010 OD U min–1 for intact cells and –0.00325 ± 0.00010 OD U min–1 for lysed cells. Release of reducing sugars in intact cells (5.3940 ± 0.1713 μmol h–1), and lysed cells (0.8396 ± 0.2251 μmol h–1) was measured. Digestive gland treatment significantly reduced cell viability (near 100%), relative to the control. Electron microscopy shows that the cell wall of Y. lipolytica exposed to the digestive gland enzymes was severely damaged. Shrimp diet containing Y. lipolytica resulted in significantly higher weight gain and specific growth rate of whiteleg shrimp."
Editor
Central Fisheries Research Institute, Trabzon, Turkey
Fecha de publicación
2018
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Turkish Journal of Fisheries and Aquatic Sciences
Idioma
Inglés
Repositorio Orígen
Repositorio Institucional CIBNOR
Descargas
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