Title
Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains
Author
CARLOS ABRAHAM GUERRERO RUIZ
Contributor
Alexei Licea Navarro (Collaborator)
Ricardo Alberto González Sánchez (Collaborator)
Marcial Leonardo Lizárraga Partida (Collaborator)
Access level
Open Access
Alternative identifier
doi: https://doi.org/10.1371/journal.pone.0220385
Subjects
Article, bacterial gene, bacterial strain, bacterial virulence, comparative study, controlled study, gene cluster, gene identification, genomic island, genotype, nonhuman, phylogenetic tree, sequence analysis, strain identification, Vibrio vulnificus - (SCOPUS) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) GENÉTICA - (CTI) GENÉTICA - (CTI)
Summary or description
Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Publisher
Gabriel Moreno-Hagelsieb, Wilfrid Laurier University, Canada
Publish date
2019
Publication type
Article
Publication version
Published Version
Information Resource
Format
application/pdf
Source
PLoS ONE, Vol.14, No.7, Pags. 1-13
Language
English
Citation suggestion
Guerrero A, Licea-Navarro AF, González-Sánchez R, Lizárraga-Partida ML (2019) Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains. PLoS ONE 14(7): e0220385. https://doi.org/10.1371/journal.pone.0220385
Source repository
Repositorio Institucional CICESE
Downloads
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