Title

Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains

Author

CARLOS ABRAHAM GUERRERO RUIZ

Contributor

Alexei Licea Navarro (Collaborator)

Ricardo Alberto González Sánchez (Collaborator)

Marcial Leonardo Lizárraga Partida (Collaborator)

Access level

Open Access

Alternative identifier

doi: https://doi.org/10.1371/journal.pone.0220385

Summary or description

Whole-genome sequences of Vibrio vulnificus clinical genotype (C-genotype) from the CICESE Culture Collection, isolated from oysters, were compared with reference sequences of CMCP6 and YJ016 V. vulnificus C-genotype strains of clinical origin. The RAST web server estimated the whole genome to be ~4.8 Mb in CICESE strain 316 and ~4.7 Mb in CICESE strain 325. No plasmids were detected in the CICESE strains. Based on a phylogenetic tree that was constructed with the whole-genome results, we observed high similarity between the reference sequences and oyster C-genotype isolates and a sharp contrast with environmental genotype (E-genotype) reference sequences, indicating that the differences between the C- and E-genotypes do not necessarily correspond to their isolation origin. The CICESE strains share 3488 genes (63.2%) with the YJ016 strain and 3500 genes (63.9%) with the CMCP6 strain. A total of 237 pathogenicity associated genes were selected from reference clinical strains, where—92 genes were from CMCP6, 126 genes from YJ016, and 19 from MO6-24/ O; the presence or absence of these genes was recorded for the CICESE strains. Of the 92 genes that were selected for CMCP6, 67 were present in both CICESE strains, as were as 86 of the 126 YJ016 genes and 13 of the 19 MO6-24/O genes. The detection of elements that are related to virulence in CICESE strains—such as the RTX gene cluster, vvhA and vvpE, the type IV pili cluster, the XII genomic island, and the viuB genes, suggests that environmental isolates with the C-genotype, have significant potential for infection. © 2019 Guerrero et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Publisher

Gabriel Moreno-Hagelsieb, Wilfrid Laurier University, Canada

Publish date

2019

Publication type

Article

Publication version

Published Version

Format

application/pdf

Source

PLoS ONE, Vol.14, No.7, Pags. 1-13

Language

English

Citation suggestion

Guerrero A, Licea-Navarro AF, González-Sánchez R, Lizárraga-Partida ML (2019) Whole-genome comparison between reference sequences and oyster Vibrio vulnificus C-genotype strains. PLoS ONE 14(7): e0220385. https://doi.org/10.1371/journal.pone.0220385

Source repository

Repositorio Institucional CICESE

Downloads

20

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