Título
Dual function of EDTA with silver nanoparticles for root canal treatment–A novel modification
Autor
JUAN MANUEL MARTINEZ ANDRADE
Colaborador
Miguel Avalos-Borja (Colaborador)
VILCHIS-NESTOR ALFREDO RAFAEL (Colaborador)
Luis Octavio Sanchez Vargas (Colaborador)
Ernestina Castro-Longoria (Colaborador)
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.1371/journal.pone.0190866
Materias
edetic acid, silver nanoparticle, edetic acid, metal nanoparticle, silver, antibacterial activity, antibiotic sensitivity, antifungal activity, antimicrobial activity, Article, atomic absorption spectrometry, atomic force microscopy, biofilm, bovine, - (SCOPUS) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) MICROBIOLOGÍA - (CTI) MICROBIOLOGÍA - (CTI)
Resumen o descripción
The chelating and antimicrobial capacity of a novel modification of 17% EDTA with silver nanoparticles (AgNPs) (EDTA-AgNPs) was evaluated in-vitro for root canal treatment (RCT). The EDTA-AgNPs solution was characterized by UV-Vis spectroscopy, ?-potential and high-resolution transmission electron microscopy (HRTEM). Antimicrobial capacity was evaluated against Candida albicans and Staphylococcus aureus in planktonic and biofilm cells by broth macrodilution (24 h) and XTT assays, (1, 10 and 30 min) respectively. The chelating capacity of EDTA-AgNPs was assessed indirectly (smear layer removal) and directly (demineralizing effect) in bovine dentin at two silver concentrations, 16 and 512 ?g/ ml at 1 and 10 minutes of exposure time. Smear layer removal was evaluated by atomic force microscopy (AFM) and scanning electron microscopy (SEM). The demineralizing effect was determined by atomic absorption spectroscopy (AAS), microhardness test (MH) and X-ray diffractometer (XRD). Synthesized AgNPs were quasi-spherical in shape with an average size of 13.09 ± 8.05 nm. 17% EDTA-AgNPs was effective to inhibit C. albicans and S. aureus in planktonic and biofilm cultures. The smear layer removal and demineralizing effect were similar between 17% EDTA-AgNPs and 17% EDTA treatments. The 17% EDTA-AgNPs solution proved to be an effective antimicrobial agent, and has a similar chelating capacity to 17% EDTA alone. These in-vitro studies strongly suggest that EDTA-AgNPs could be used for effective smear layer removal, having an antimicrobial effect at the same time during RCT. © 2018 Martinez-Andrade et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Editor
Amitava Mukherjee, VIT University, India
Fecha de publicación
2018
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
PLoS ONE, Vol.13, No.1, Pags. 1-19
Idioma
Inglés
Sugerencia de citación
Martinez-Andrade J.M., Avalos-Borja M., Vilchis-Nestor A.R., Sanchez-Vargas L.O., Castro-Longoria E.2018. Dual function of EDTA with silver nanoparticles for root canal treatment–A novel modification. PLoS ONE 13(1): e0190866. https://doi.org/10.1371/journal.pone.0190866
Repositorio Orígen
Repositorio Institucional CICESE
Descargas
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