Un protocolo de embriogénesis somática para la regeneración y caracterización in vitro de Laelia anceps ssp. dawsonii
A protocol of somatic embryogenesis for the in vitroregeneration and characterization of Laelia anceps ssp. dawsonii
Hilda Eulalia Lee Espinosa
ANTONIO LAGUNA CERDA
Joaquín Murguía González
Lourdes Georgina Iglesias Andreu
BENJAMIN GARCIA ROSAS
DIANA ESCOBEDO LOPEZ
YOLANDA MARIA MARTINEZ OCAMPO
FELIPE ALONSO BARREDO POOL
NANCY SANTANA BUZZY
Summary or description
Laelia anceps ssp. dawsonii is one of the most appreciated Mexican wild orchids, with atractive characteristics for ornamental potential according to international standards. It has become an important economic species, subjected to excessive collection and risk of extinction. To ensure its conservation, it is necessary to develop efficient propagation protocols which allow its sustainable utilization and reduce collection. Somatic embryogenesis is an efficient technology for in vitro plant multiplication. In this study we evaluated in vitro conditions for developing a somatic embryogenesis protocol for Laelia anceps ssp. dawsonii. Embryogenic calli was induced from mature seeds, in a Murashige and Skoog medium supplemented with multiple combinations of naphthalene acetic acid (NAA), 6-benzylaminopurine (BAP), kinetin (Kin) and indole 3 acetic acid (IAA). The combination NAA+BAP+IAA (2.0 mg L-1 each) was optimal for callus induction, producing 611 embryoids in a 16 h photoperiod (33.8 µmol m-2 s-1) when subcultured every 45 d on the same medium. This conditions allowed for complete plantlet development. In approximately three months, plantlets achieved 4-5 cm in a V&W medium supplemented with BAP (2 mg L-1), AIA (1 mg L-1) and 0.2 % activated charcoal; after 30 d plantlets were acclimatized in a greenhouse with 95 % survival rate.
Revista Fitotecnia Mexicana, 33(4), 323-332, 2010
Santana Buzzy, N. (2010). A protocol of somatic embryogenesis for the in vitro regeneration and characterization of Laelia anceps ssp. Dawsonii. Revista Fitotecnia Mexicana, 33(4), 323-332.
Lee Espinosa, H. E., Laguna Cerda, A., Murguia Gonzalez, J., Iglesias-Andreu, L., Garcia Rosas, B., Escobedo Lopez, D., ...
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