Título

Molecular cloning and functional analysis of a Na + -insensitive K + transporter of Capsicum chinense Jacq

Autor

NANCY RUIZ LAU

Emanuel Bojorquez Quintal

BEGOÑA BENITO CASADO

Ileana de la Caridad Echevarría Machado

Lucila Aurelia Sánchez Cach

María de Fátima Medina Lara

Manuel Martínez Estévez

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

datasetDOI/10.3389/fpls.2016.01980

Materias

CAPSICUM CHINENSE - (AUTORES) HAK-TYPE TRANSPORTER - (AUTORES) K+-STARVATION - (AUTORES) POTASSIUM - (AUTORES) ROOTS - (AUTORES) SODIUM - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) BIOLOGÍA Y QUÍMICA - (CTI)

Resumen o descripción

High-affinity K+ (HAK) transporters are encoded by a large family of genes and are ubiquitous in the plant kingdom. These HAK-type transporters participate in low- and high-affinity potassium (K+) uptake and are crucial for the maintenance of K+ homeostasis under hostile conditions. In this study, the full-length cDNA of CcHAK1 gene was isolated from roots of the habanero pepper (Capsicum chinense). CcHAK1 expression was positively regulated by K+ starvation in roots and was not inhibited in the presence of NaCl. Phylogenetic analysis placed the CcHAK1 transporter in group I of the HAK K+ transporters, showing that it is closely related to Capsicum annuum CaHAK1 and Solanum lycopersicum LeHAK5. Characterization of the protein in a yeast mutant deficient in high-affinity K+ uptake (WΔ3) suggested that CcHAK1 function is associated with high-affinity K+ uptake, with Km and Vmax for Rb of 50 μM and 0.52 nmol mg−1 min−1, respectively. K+ uptake in yeast expressing the CcHAK1 transporter was inhibited by millimolar concentrations of the cations ammonium (NH+4) and cesium (Cs+) but not by sodium (Na+). The results presented in this study suggest that the CcHAK1 transporter may contribute to the maintenance of K+ homeostasis in root cells in C. chinense plants undergoing K+-deficiency and salt stress.

Fecha de publicación

2019

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1540

Formato

application/pdf

Fuente

Frontiers in plant science, 7, 1980.

Idioma

Inglés

Relación

&

Martínez-Estévez, M. (2016). Molecular cloning and functional analysis of a Na+-insensitive K+ transporter of Capsicum chinense Jacq. Frontiers in plant science, 7, 1980.

Sugerencia de citación

Ruiz-Lau, N., Bojórquez-Quintal, E., Benito, B., Echevarría-Machado, I., Sánchez-Cach, L. A., Medina-Lara, M. D. F.,

Repositorio Orígen

Repositorio Institucional CICY

Descargas

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