Título
In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease
Autor
IVAN ISIDRO CORDOVA LARA
Phil Jones
Nigel A. Harrison
Carlos Mariano Oropeza Salín
Nivel de Acceso
Acceso Abierto
Referencia de datos
datasetDOI/https://doi.org/10.1046/j.1364-3703.2003.00152.x
Materias
Resumen o descripción
DNA of the lethal yellowing (LY) phytoplasma was detected in 13 of 72 embryos from fruits of four diseased Atlantic tall coconut palms by polymerase chain reaction (PCR) assays employing phytoplasma universal rRNA primer pair P1/P7, nested LY group‐specific rRNA primer pair 503f/LY16Sr or LY phytoplasma‐specific nonribosomal primer pair LYF1/R1. Phytoplasma distribution in sectioned tissues from six PCR positive embryos was determined by in situ PCR and digoxigenin‐11‐deoxy‐UTP (Dig) labelling of amplification products. Dig‐labeled DNA products detected by colourimetric assay were clearly evident on sections from the same three embryos investigated in detail by in situ PCRs employing primer pairs P1/P7 or LYF1/R1. Deposition of blue–green stain on sections as a result of each assay was restricted to areas of the embryos corresponding to the plumule and cells ensheathing it. By comparison, similarly treated embryo sections derived from fruits of a symptomless Atlantic tall coconut palm were consistently devoid of any stain. Presence of phytoplasma DNA in embryo tissues suggests the possible potential for seed transmission which remains to be demonstrated.
Fecha de publicación
2003
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Molecular Plant Pathology, 4(2), 99-108, 2003.
Idioma
Inglés
Relación
&
Oropeza, C. (2003). In situ PCR detection of phytoplasma DNA in embryos from coconut palms with lethal yellowing disease. Molecular Plant Pathology, 4(2), 99-108.
Sugerencia de citación
Cordova, I., Jones, P., Harrison, N. A.,
Repositorio Orígen
Repositorio Institucional CICY
Descargas
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