Título
Enterobacter cloacae, an endophyte that establishes a nutrient-transfer symbiosis with banana plants and protects against the black Sigatoka pathogen
Autor
GLORIA MARGARITA MACEDO RAYGOZA
BENJAMIN VALDEZ SALAS
Fernanda Manso Prado
Katia R. Prieto
Lydia F. Yamaguchi
Massuo Jorge Kato
Blondy Beatriz Canto Canché
MONICA CARRILLO BELTRAN
Paolo Di Mascio
James F. White
MIGUEL JUAN BELTRAN GARCIA
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://oi.org/10.3389/fmicb.2019.00804
Referencia de datos
datasetDOI/ https://oi.org/10.3389/fmicb.2019.00804
Materias
ENDOPHYTES - (AUTORES) ENTEROBACTER CLOACAE - (AUTORES) KLEBSIELLA PNEUMONIAE - (AUTORES) NUTRIENT-TRANSFE - (AUTORES) 15N-LABELED PHEOPHYTIN - (AUTORES) SYMBIOSIS, PSEUDOCERCOSPORA FIJIENSIS - (AUTORES) BANANA - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA MOLECULAR - (CTI) BIOLOGÍA MOLECULAR DE PLANTAS - (CTI) BIOLOGÍA MOLECULAR DE PLANTAS - (CTI)
Resumen o descripción
Banana (Musa spp.) is an important crop worldwide, but black Sigatoka disease caused by the fungus Pseudocercospora fijiensis threatens fruit production. In this work, we examined the potential of the endophytes of banana plants Enterobacter cloacae and Klebsiella pneumoniae, as antagonists of P. fijiensis and support plant growth in nutrient limited soils by N-transfer. The two bacterial isolates were identified by MALDI-TOF mass spectrometry and corroborated by 16S rRNA sequence analysis. Both bacteria were positive for beneficial traits such as N-fixation, indole acetic acid production, phosphate solubilization, negative for 1-aminocyclopropane 1-carboxylic acid deaminase and were antagonistic to P. fijiensis. To measure the effects on plant growth, the two plant bacteria and an E. coli strain (as non-endophyte), were inoculated weekly for 60 days as active cells (AC) and heat-killed cells (HKC) into plant microcosms without nutrients and compared to a water only treatment, and a mineral nutrients solution (MMN) treatment. Bacterial treatments increased growth parameters and prevented accelerated senescence, which was observed for water and mineral nutrients solution (MMN) treatments used as controls. Plants died after the first 20 days of being irrigated with water; irrigation with MMN enabled plants to develop some new leaves, but plants lost weight (−30%) during the same period. Plants treated with bacteria showed good growth, but E. cloacae AC treated plants had significantly greater biomass than the E. cloacae HKC. After 60 days, plants inoculated with E. cloacae AC showed intracellular bacteria within root cells, suggesting that a stable symbiosis was established. To evaluate the transference of organic N from bacteria into the plants, the 3 bacteria were grown with 15NH4Cl or Na15NO3 as the nitrogen source. The 15N transferred from bacteria to plant tissues was measured by pheophytin isotopomer abundance. The relative abundance of the isotopomers m/z 872.57, 873.57, 874.57, 875.57, 876.57 unequivocally demonstrated that plants acquired 15N atoms directly from bacterial cells, using them as a source of N, to support plant growth in restricted nutrient soils. E. cloacae might be a new alternative to promote growth and health of banana crops.
Fecha de publicación
2019
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Frontiers in microbiology, 10, 804
Idioma
Inglés
Relación
&
Beltrán-García, M. J. (2019). Enterobacter cloacae, an endophyte that establishes a nutrient-transfer symbiosis with banana plants and protects against the black Sigatoka pathogen. Frontiers in microbiology, 10, 804
Sugerencia de citación
Macedo-Raygoza, G. M., Valdez-Salas, B., Prado, F. M., Prieto, K. R., Yamaguchi, L. F., Kato, M. J., ...
Repositorio Orígen
Repositorio Institucional CICY
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