Título

Molecular identification of selected streptomyces strains isolated from Mexican tropical soils and their anti-Candida activity

Autor

DIANA YAMILY ESCALANTE RENDIZ

SUSANA DEL CARMEN DE LA ROSA GARCIA

RAUL TAPIA TUSSELL

Jesús Martin

Fernando Reyes

Francisca Vicente Perez

MARIA MARCELA GAMBOA ANGULO

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de datos

datasetDOI/https://doi.org/10.3390/ijerph16111913

Materias

STREPTOMYCES PARVISPOROGENES - (AUTORES) ANTIFUNGAL - (AUTORES) CANDIDA ALBICANS - (AUTORES) CARBAZOMYCIN G - (AUTORES) PKS AND NRPS GENES - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA CELULAR - (CTI) CITOGENÉTICA - (CTI) CITOGENÉTICA - (CTI)

Resumen o descripción

The increasing incidence of Candida albicans infections and resistance to current antifungal therapies has led to the search for new and more effective antifungal compounds. Actinobacterial species from the Streptomyces genus are recognized as some of the major producers of antimicrobial compounds. Therefore, the aims of this study were: (1) the identification of Streptomyces strains isolated from Mexican tropical acidic soils, (2) the evaluation of their antifungal activity on C. albicans, and (3) the exploration of the presence of polyketide synthase genes in their genome and antifungal secondary metabolites in their extracts. Four actinobacterial strains, isolated from previously unexplored soils with antibacterial antecedents, were selected. These strains were identified as Streptomyces angustmyceticus S6A-03, Streptomyces manipurensis S3A-05 and S3A-09, and Streptomyces parvisporogenes S2A-04, according to their molecular analyses. The ethanol extract of the lyophilized supernatant of S. parvisporogenes displayed the most interesting antifungal activity against C. albicans, with a minimum inhibitory concentration (MIC) of 0.5 mg/mL. Type I polyketide synthase (PKS-I) and non-ribosomal peptide synthase (NRPS) genes were detected in all strains. In addition, type II PKS genes (PKS-II) were also found in S. manipurensis S3A-05 and S. parvisporogenes. LC-UV-HRMS analysis of the active organic extract of S. parvisporogenes indicated the presence of the known antifungal compound carbazomycin G as the major component.

Fecha de publicación

2019

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1748

Formato

application/pdf

Fuente

International journal of environmental research and public health, 16(11), 1913, 2019

Idioma

Inglés

Relación

&

Gamboa-Angulo, M. (2019). Molecular Identification of Selected Streptomyces Strains Isolated from Mexican Tropical Soils and their Anti-Candida Activity. International journal of environmental research and public health, 16(11), 1913. Escalante-Réndiz, D., de-la-Rosa-García, S., Tapia-Tussell, R., Martín, J., Reyes, F., Vicente, F.,

&

Gamboa-Angulo, M. (2019). Molecular Identification of Selected Streptomyces Strains Isolated from Mexican Tropical Soils and their Anti-Candida Activity. Interna

Sugerencia de citación

Escalante-Réndiz, D., de-la-Rosa-García, S., Tapia-Tussell, R., Martín, J., Reyes, F., Vicente, F.,

Repositorio Orígen

Repositorio Institucional CICY

Descargas

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