Title
The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
Author
SERGIO GARCIA LAYNES
VIRGINIA AURORA HERRERA VALENCIA
Lilia Guadalupe Tamayo Torres
VERONICA LIMONES BRIONES
FELIPE ALONSO BARREDO POOL
FRAY MARTIN BAAS ESPINOLA
Angel Alpuche-Solis
CARLOS ALBERTO PUCH HAU
SANTY PERAZA ECHEVERRIA
Access level
Open Access
Dataset reference
datasetDOI/https://doi.org/10.3390/genes13101891
Subjects
BANANA - (AUTORES) TRANSCRIPTION FACTOR - (AUTORES) WRKY - (AUTORES) DEFENSE PHYTOHORMONES - (AUTORES) SALICYLIC ACID - (AUTORES) METHYL JASMONATE - (AUTORES) SAR - (AUTORES) ISR - (AUTORES) BROAD-SPECTRUM RESISTANCE - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) GENÉTICA - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI)
Summary or description
WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.
Publish date
2022
Publication type
Article
Publication version
Published Version
Information Resource
Format
application/pdf
Source
Genes, 13(10), 1891, 2022.
Language
English
Citation suggestion
García-Laynes, S.; Herrera-Valencia, V.A.; Tamayo-Torres, L.G.; Limones-Briones, V.; Barredo-Pool, F.A.; Baas-Espinola, F.M.; Alpuche-Solís, A.G.; Puch-Hau, C.; Peraza-Echeverria, S. The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones. Genes 2022, 13, 1891. https://doi.org/10.3390/genes13101891
Source repository
Repositorio Institucional CICY
Downloads
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