Título
The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones
Autor
SERGIO GARCIA LAYNES
VIRGINIA AURORA HERRERA VALENCIA
Lilia Guadalupe Tamayo Torres
VERONICA LIMONES BRIONES
FELIPE ALONSO BARREDO POOL
FRAY MARTIN BAAS ESPINOLA
Angel Alpuche-Solis
CARLOS ALBERTO PUCH HAU
SANTY PERAZA ECHEVERRIA
Nivel de Acceso
Acceso Abierto
Referencia de datos
datasetDOI/https://doi.org/10.3390/genes13101891
Materias
BANANA - (AUTORES) TRANSCRIPTION FACTOR - (AUTORES) WRKY - (AUTORES) DEFENSE PHYTOHORMONES - (AUTORES) SALICYLIC ACID - (AUTORES) METHYL JASMONATE - (AUTORES) SAR - (AUTORES) ISR - (AUTORES) BROAD-SPECTRUM RESISTANCE - (AUTORES) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) GENÉTICA - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI) GENÉTICA MOLECULAR DE PLANTAS - (CTI)
Resumen o descripción
WRKY transcription factors (TFs) play key roles in plant defense responses through phytohormone signaling pathways. However, their functions in tropical fruit crops, especially in banana, remain largely unknown. Several WRKY genes from the model plants rice (OsWRKY45) and Arabidopsis (AtWRKY18, AtWRKY60, AtWRKY70) have shown to be attractive TFs for engineering disease resistance. In this study, we isolated four banana cDNAs (MaWRKY18, MaWRKY45, MaWRKY60, and MaWRKY70) with homology to these rice and Arabidopsis WRKY genes. The MaWRKY cDNAs were isolated from the wild banana Musa acuminata ssp. malaccensis, which is resistant to several diseases of this crop and is a progenitor of most banana cultivars. The deduced amino acid sequences of the four MaWRKY cDNAs revealed the presence of the conserved WRKY domain of ~60 amino acids and a zinc-finger motif at the N-terminus. Based on the number of WRKY repeats and the structure of the zinc-finger motif, MaWRKY18 and MaWRKY60 belong to group II of WRKY TFs, while MaWRKY45 and MaWRKY70 are members of group III. Their corresponding proteins were located in the nuclei of onion epidermal cells and were shown to be functional TFs in yeast cells. Moreover, expression analyses revealed that the majority of these MaWRKY genes were upregulated by salicylic acid (SA) or methyl jasmonate (MeJA) phytohormones, although the expression levels were relatively higher with MeJA treatment. The fact that most of these banana WRKY genes were upregulated by SA or MeJA, which are involved in systemic acquired resistance (SAR) or induced systemic resistance (ISR), respectively, make them interesting candidates for bioengineering broad-spectrum resistance in this crop. © 2022 by the authors.
Fecha de publicación
2022
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
Genes, 13(10), 1891, 2022.
Idioma
Inglés
Sugerencia de citación
García-Laynes, S.; Herrera-Valencia, V.A.; Tamayo-Torres, L.G.; Limones-Briones, V.; Barredo-Pool, F.A.; Baas-Espinola, F.M.; Alpuche-Solís, A.G.; Puch-Hau, C.; Peraza-Echeverria, S. The banana MaWRKY18, MaWRKY45, MaWRKY60 and MaWRKY70 genes encode functional transcription factors and display differential expression in response to defense phytohormones. Genes 2022, 13, 1891. https://doi.org/10.3390/genes13101891
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