Title
The search for cryptic L-Rhamnosyltransferases on the Sporothrix schenckii genome
Author
Hector M. Mora-Montes
Karina García-Gutiérrez
Laura Cristina García Carnero
Nancy Lozoya-Perez
Jorge Humberto Ramírez Prado
Access level
Open Access
Dataset reference
datasetDOI/https://doi.org/10.3390/jof8050529
Subjects
Summary or description
The fungal cell wall is an attractive structure to look for new antifungal drug targets and for understanding the host-fungus interaction. Sporothrix schenckii is one of the main causative agents of both human and animal sporotrichosis and currently is the species most studied of the Sporothrix genus. The cell wall of this organism has been previously analyzed, and rhamnoconjugates are signature molecules found on the surface of both mycelia and yeast-like cells. Similar to other reactions where sugars are covalently linked to other sugars, lipids, or proteins, the rhamnosylation process in this organism is expected to involve glycosyltransferases with the ability to transfer rhamnose from a sugar donor to the acceptor molecule, i.e., rhamnosyltransferases. However, no obvious rhamnosyltransferase has thus far been identified within the S. schenckii proteome or genome. Here, using a Hidden Markov Model profile strategy, we found within the S. schenckii genome five putative genes encoding for rhamnosyltransferases. Expression analyses indicated that only two of them, named RHT1 and RHT2, were significantly expressed in yeast-like cells and during interaction with the host. These two genes were heterologously expressed in Escherichia coli, and the purified recombinant proteins showed rhamnosyltransferase activity, dependent on the presence of UDP-rhamnose as a sugar donor. To the best of our knowledge, this is the first report about rhamnosyltransferases in S. schenckii. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Publish date
2022
Publication type
Article
Publication version
Published Version
Information Resource
Format
application/pdf
Source
Journal of Fungi, 8(5), 529, 2022.
Language
English
Citation suggestion
Mora-Montes, H.M.; García-Gutiérrez, K.; García-Carnero, L.C.; Lozoya-Pérez, N.E.; Ramirez-Prado, J.H. The search for cryptic L-Rhamnosyltransferases on the Sporothrix schenckii genome. J. Fungi 2022, 8, 529. https://doi.org/10.3390/jof8050529
Source repository
Repositorio Institucional CICY
Downloads
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