Título
Antimicrobial evaluation of extracts obtained from tropical micromycetes against phytopathogens
Autor
MARTIN PABLO ANTONIO MORENO PEREZ
MARIA MARCELA GAMBOA ANGULO
GABRIELA PATRICIA HEREDIA ABARCA
BLONDY BEATRIZ CANTO CANCHE
CECILIA MONICA RODRIGUEZ GARCIA
Irma Leticia Medina Baizabal
LETICIA PERAZA ECHEVERRIA
Nivel de Acceso
Acceso Abierto
Materias
Resumen o descripción
Nowadays, it is highly necessary to find more and safer agrochemicals. In this sense, micromycetes are an important source of natural products which could be used to control plant diseases. Therefore with the aims to contribute in this searching of natural products with antimicrobial applications, a total of 49 fungal strains were isolated from the Yucatan Peninsula. These fungi were cultured in fermented rice, their respective organic extracts macerated in ethyl acetate (EAE) and methanol (ME) were obtained and tested against five fungal pathogens of agricultural importance. These included Alternaria chrysanthemi, Colletotrichum gloeosporioides, Mycosphaerella fijiensis, and the bacteria Erwinia carotovora and Xanthomonas campestris using microdilution assays. The 69% of fungal extracts showed antifungal or antibacterial (2000 and 200 µg/mL, respectively) against at least one of the tested target. Strains Penicillium sp. OSE-61, Fusarium sp. OH2-30, Hypocrea lixii OSN-37 and Rhizoctonia solani OSE-73 showed activity against at least three of the five study pathogens. The most active EAE were partitioned, and its low (A), medium (B) polarity and precipitated (C) were obtained and assessed. The fraction A from Fusarium KS-15 displayed the lowest Minimum Inhibitory Concentration (MIC ≤25 µg/mL) and bactericide effect on X. campestris; fraction B of Penicillium sp. OSE-61 was relatively greater than the previous on A. chrysanthemi (MIC ≤500 µg/mL). Last fraction also exhibited good inhibitory effect on C. gloeosporioides. Finally, fraction B of H. lixii OSN-37 displayed promissory antifungal effect on C. gloeosporioides y M. fijiensis (MIC=1000 µg/mL). This research contributes to enrich the limited knowledge on the biological activity of native anamorphic fungi of the sinkholes of Yucatan and its potential use in biotechnological applications in agriculture.
Fecha de publicación
2016
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Fuente
PHYTON-International Journal of Experimental Botany. 85, 7-14, 2016
Idioma
Inglés
Relación
&
Peraza, E. (2016). Antimicrobial evaluation of extracts obtained from tropical micromycetes against phytopathogens. Phyton (Buenos Aires), 85, 7-14.
Sugerencia de citación
Moreno, P., Gamboa, A., Heredia, G., Canto, C., Rodríguez, G., Medina, B.,
Repositorio Orígen
Repositorio Institucional CICY
Descargas
1078