Título

Effect to different treatments on the ability of a-lactalbumin to form nanoparticles

Autor

IZLIA JAZHEEL ARROYO MAYA

MARIBEL CORNEJO MAZON

GUSTAVO FIDEL GUTIERREZ LOPEZ

ANDRES HERNANDEZ ARANA

HUMBERTO HERNANDEZ SANCHEZ

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Referencia de publicación

ISSN/0022-0302

Referencia de datos

doi: https://www.journalofdairyscience.org/article/S0022-0302(12)00625-X/fulltext

Materias

BIOLOGÍA Y QUÍMICA - (CTI) Nanopartículas - Análisis Asociación molecular Lactalbúmina

Resumen o descripción

Nanoparticles of bovine α-lactalbumin (α-LA) prepared by desolvation and glutaraldehyde crosslinking are promising carriers for bioactive compounds in foods. The objective of this work was to study the effect of changes in hydrophobic interactions by using different desolvating agents (acetone, ethanol, or isopropanol) and the use of a heat or high-pressure treatment step before the desolvation process on the size, structure, and properties of α-LA nanoparticles. In all cases, a high average particle yield of 99.63% was obtained. Smaller sizes (152.3 nm) can be obtained with the use of acetone as the desolvating agent and without any pretreatment. This is the first time that α-LA nanoparticles in the size range of 100 to 200 nm have been obtained. These nanoparticles, with an isoelectric point of 3.61, are very stable at pH values >4.8, based on their ζ-potential, although their antioxidant activity is weak. The use of the desolvating agent with the smallest polarity index (isopropanol) produced the largest particles (293.4 to 324.9 nm) in all cases. These results support the idea that controlling hydrophobic interactions is a means to control the size of α-LA nanoparticles. No effect of pretreatment on nanoparticle size could be detected. All types of nanoparticles were easily degraded by the proteolytic enzymes assayed.

Journal of Dairy Science

Editor

Champaign, Ill. : American Dairy Science Association

Fecha de publicación

21 de abril de 2020 21 de abril de 2020 2012

Tipo de publicación

Artículo

Recurso de información

http://ilitia.cua.uam.mx:8080/jspui/handle/123456789/379

Journal of Dairy Science, vol. 95, núm. 11, november, 2012

Formato

application/pdf

Idioma

Inglés

Repositorio Orígen

Concentración de Recursos de Información Científica y Académica, UAM Cuajimalpa

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