Título
Effect to different treatments on the ability of a-lactalbumin to form nanoparticles
Autor
IZLIA JAZHEEL ARROYO MAYA
MARIBEL CORNEJO MAZON
GUSTAVO FIDEL GUTIERREZ LOPEZ
ANDRES HERNANDEZ ARANA
HUMBERTO HERNANDEZ SANCHEZ
Nivel de Acceso
Acceso Abierto
Referencia de publicación
ISSN/0022-0302
Referencia de datos
doi: https://www.journalofdairyscience.org/article/S0022-0302(12)00625-X/fulltext
Resumen o descripción
Nanoparticles of bovine α-lactalbumin (α-LA) prepared by desolvation and glutaraldehyde crosslinking are promising carriers for bioactive compounds in foods. The objective of this work was to study the effect of changes in hydrophobic interactions by using different desolvating agents (acetone, ethanol, or isopropanol) and the use of a heat or high-pressure treatment step before the desolvation process on the size, structure, and properties of α-LA nanoparticles. In all cases, a high average particle yield of 99.63% was obtained. Smaller sizes (152.3 nm) can be obtained with the use of acetone as the desolvating agent and without any pretreatment. This is the first time that α-LA nanoparticles in the size range of 100 to 200 nm have been obtained. These nanoparticles, with an isoelectric point of 3.61, are very stable at pH values >4.8, based on their ζ-potential, although their antioxidant activity is weak. The use of the desolvating agent with the smallest polarity index (isopropanol) produced the largest particles (293.4 to 324.9 nm) in all cases. These results support the idea that controlling hydrophobic interactions is a means to control the size of α-LA nanoparticles. No effect of pretreatment on nanoparticle size could be detected. All types of nanoparticles were easily degraded by the proteolytic enzymes assayed.
Journal of Dairy Science
Editor
Champaign, Ill. : American Dairy Science Association
Fecha de publicación
21 de abril de 2020 21 de abril de 2020 2012
Tipo de publicación
Artículo
Recurso de información
http://ilitia.cua.uam.mx:8080/jspui/handle/123456789/379
Journal of Dairy Science, vol. 95, núm. 11, november, 2012
Formato
application/pdf
Idioma
Inglés
Repositorio Orígen
Concentración de Recursos de Información Científica y Académica, UAM Cuajimalpa
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