Título

Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins

Autor

MARIA GLORIA SOLIS GUZMAN

Gerardo Rafael Argüello Astorga

JOSE LOPEZ BUCIO

LEON FRANCISCO RUIZ HERRERA

Joel Edmundo López Meza

LENIN SANCHEZ CALDERON

YAZMIN CARREON ABUD

MIGUEL MARTINEZ TRUJILLO

Nivel de Acceso

Acceso Abierto

Licencia

http://creativecommons.org/licenses/by-nc-nd/4.0

Identificador alterno

doi: http://dx.doi.org/10.1590/1678-4685-gmb-2016-0223

Materias

Spen - (AUTOR) Arabidopsis - (AUTOR) Vascular bundle - (AUTOR) Expression - (AUTOR) BIOLOGÍA Y QUÍMICA - (CTI) CIENCIAS DE LA VIDA - (CTI) BIOLOGÍA MOLECULAR - (CTI) BIOLOGÍA MOLECULAR - (CTI)

Resumen o descripción

"Proteins of the Split ends (Spen) family are characterized by an N-terminal domain, with one or more RNA recognition motifs and a SPOC domain. In Arabidopsis thaliana, the Spen protein FPA is involved in the control of flowering time as a component of an autonomous pathway independent of photoperiod. The A. thaliana genome encodes another gene for a putative Spen protein at the locus At4g12640, herein named AtSpen2. Bioinformatics analysis of the AtSPEN2 SPOC domain revealed low sequence similarity with the FPA SPOC domain, which was markedly lower than that found in other Spen proteins from unrelated plant species. To provide experimental information about the function of AtSpen2, A. thaliana plants were transformed with gene constructs of its promoter region with uidA::gfp reporter genes; the expression was observed in vascular tissues of leaves and roots, as well as in ovules and developing embryos. There was absence of a notable phenotype in knockout and overexpressing lines, suggesting that its function in plants might be specific to certain endogenous or environmental conditions. Our results suggest that the function of Atspen2 diverged from that of fpa due in part to their different transcription expression pattern and divergence of the regulatory SPOC domain."

Editor

Sociedade Brasileira de Genética

Fecha de publicación

2017

Tipo de publicación

Artículo

Versión de la publicación

Versión publicada

Recurso de información

http://ipicyt.repositorioinstitucional.mx/jspui/handle/1010/1844

Formato

application/pdf

Relación

&

Martínez-Trujillo, Miguel. (2017). Expression analysis of the Arabidopsis thaliana AtSpen2 gene, and its relationship with other plant genes encoding Spen proteins. Genetics and Molecular Biology, 40(3), 643-655. Epub August 28, 2017

Sugerencia de citación

Solís-Guzmán, María Gloria, Argüello-Astorga, Gerardo, López-Bucio, José, Ruiz-Herrera, León Francisco, López-Meza, Joel, Sánchez-Calderón, Lenin, Carreón-Abud, Yazmín,

Repositorio Orígen

Repositorio IPICYT

Descargas

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