Título
Silencing of OCH1 unveils the role of Sporothrix schenckii N-linked glycans during the host-fungus interaction
Autor
NANCY EDITH LOZOYA PEREZ
J Sergio Casas Flores
José Roberto Fogaça de Almeida
JOSE ASCENCION MARTINEZ ALVAREZ
Luz Adriana López Ramírez
Grasielle Jannuzzi
JOSE ELIAS TRUJILLO ESQUIVEL
EINE ESTRADA MATA
sandro almeida
Bernardo Franco
Leila Maria Lópes Bezerra
HECTOR MANUEL MORA MONTES
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.2147/IDR.S185037
Materias
Cell wall - (AUTOR) Protein glycosylation - (AUTOR) Sporotrichosis - (AUTOR) Gene silencing - (AUTOR) Host-fungus interplay - (AUTOR) Mannosyltransferase - (AUTOR) MEDICINA Y CIENCIAS DE LA SALUD - (CTI) CIENCIAS MÉDICAS - (CTI) MEDICINA INTERNA - (CTI) ENFERMEDADES INFECCIOSAS - (CTI) ENFERMEDADES INFECCIOSAS - (CTI)
Resumen o descripción
"Background: Sporothrix schenckii is a neglected fungal pathogen for the human being and other mammals. In several fungal systems, Och1 is a Golgi alpha 1,6-mannosyltransferase with a key function in the synthesis of N-linked glycans; which are important elements during the host-fungus interplay. The role of OCH1 in fungal virulence seems to be species-specific, being an essential component for Candida albicans virulence and dispensable during the interaction of Aspergillus fumigatus with the host. Methods: Here, we silenced S. schenckii OCH1 and characterized the phenotype of the mutant strains. Results: The mutant strains did not show defects in the cell or colony morphology, the growth rate or the ability to undergo dimorphism; but the cell wall changed in both composition and exposure of inner components at the surface. When interacting with human monocytes, the silenced strains had a reduced ability to stimulate TNF alpha and IL-6 but stimulated higher levels of IL-10. The interaction with hitman macrophages was also altered, with reduced numbers of silenced cells phagocytosed. These strains showed virulence attenuation in both Galleria mellonella and in the mouse model of sporotrichosis. Nonetheless, the cytokine levels in infected organs did not vary significantly when compared with the wild-type strain. Conclusion: Our data demonstrate that OCH1 silencing affects different aspects of the S. schenckii-host interaction."
Editor
Dove Press Ltd
Fecha de publicación
2019
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Lozoya-Pérez NE, Casas-Flores S, de Almeida JR, Martínez-Álvarez JA, López-Ramírez LA, Jannuzzi GP, Trujillo-Esquivel E, Estrada-Mata E, Almeida SR, Franco B, Lopes-Bezerra LM, Mora-Montes HM. Silencing of OCH1 unveils the role of Sporothrix schenckii N-linked glycans during the host fungus interaction. Infect Drug Resist. 2019;12:67-85 https://doi.org/10.2147/IDR.S185037
Repositorio Orígen
Repositorio IPICYT
Descargas
286