Título
Modulation of caecal microbiome in obese mice associated with administration of amaranth or soybean protein isolates
Autor
DIANA OLGUIN CALDERON
JORGE LUIS GONZALEZ ESCOBAR
ROSALVA RIOS VILLA
ELENA DIBILDOX ALVARADO
Antonio de León Rodríguez
Ana Paulina Barba de la Rosa
Nivel de Acceso
Acceso Abierto
Identificador alterno
doi: https://doi.org/10.31883/pjfns-2019-0002
Materias
Resumen o descripción
"Obesity is defined as abnormal or excessive body fat accumulation that may have negative effects on health. Healthy diet induces a balance of gut microbiota, helping in turn to combat this metabolic disorder. Amaranth is well known because of its beneficial properties on health. but its effects on microbiota profile are still unknown. The aim of this study was to analyse the changes of gut microbiota in diet-induced obese mice due to amaranth protein consumption and to compare them with the changes due to soybean protein intake. Male C57BL/6 mice were fed for 8 weeks with regular (RD) or high-fat (HF) diet, without or with complementation with amaranth or soybean protein isolates. Morphological changes in caecum ultrathin sections were measured after hematoxylin/eosin staining. Microbiota was isolated from the caecum and 16S rRNA gene was sequenced. Caecal Short Chain Fatty Acids (SCEs) were quantified by gas chromatography. The consumption of soybean protein induced the ectopic deposition of fat in the whole intestine while amaranth proteins increased caecal crypt depth and calceiform cells number sustaining its beneficial effect on health. The count of Ruminococcacea family bacteria was increased in mice fed with HF diet, but amaranth proteins intake reduced its abundance. In turn, Lachnospiraceae bacteria abundance decreased in mice fed the Control-HF and amaranth HF diets. but increased in mice fed the soybean diets. In mice fed the soybean diets. amaranth induced the abundance of Prevotellaceae, an acetate-producing bacteria. Study results indicate that the modulation of caecal microbiota could be one of the mechanisms by which amaranth exerts its beneficial effects on health."
Editor
Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences
Fecha de publicación
2019
Tipo de publicación
Artículo
Versión de la publicación
Versión publicada
Recurso de información
Formato
application/pdf
Sugerencia de citación
Olguín-Calderón, D., González-Escobar, J. L., Ríos-Villa, R., Dibildox-Alvarado, E., De León-Rodríguez, A., Barba de la Rosa, A. P. (2019). Modulation of Caecal Microbiome in Obese Mice Associated with Administration of Amaranth or Soybean Protein Isolates. Polish Journal of Food and Nutrition Sciences, 69(1), 35-44. https://doi.org/10.31883/pjfns-2019-0002
Repositorio Orígen
Repositorio IPICYT
Descargas
142